Research Article
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Year 2024, Volume: 7 Issue: 3, 144 - 154, 03.07.2024
https://doi.org/10.3153/AR24013

Abstract

References

  • Abeliovich, A. Weisman, D. (1978). Role of heterotrophic nutrition in growth of the alga Scenedesmus obliquus in high-rate oxidation ponds, Applied and Environmental Microbiology, 35(1), 32–37. https://doi.org/10.1128/aem.35.1.32-37.1978
  • Abad, M.J., Bedoya, L.M., Bermejo, P. (2011). Marine compounds and their antimicrobial activities. Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, 51(1), 1293–1306.
  • Ben, R.D. (2012). Photosynthetic behavior of microalgae in response to environmental factors. Applied Photosynthesis, 1(1), 23-45.
  • Bhuyar, P., Rahim, M.H.A., Yusoff, M.M., Maniam, G.P., Govindan, N. (1990). A selective microalgae strain for biodiesel production in relation to higher lipid profile. Maejo International Journal of Energy and Environmental Communication, 1(2), 8–14. https://doi.org/10.54279/mijeec.v1i1.244895
  • Bhuyar, P., Yusoff, M.M., Rahim, M.H.A., Sundararaju, S., Maniam, G.P., Govindan, N. (2020). Effect of plant hormones on the production of biomass and lipid extraction for biodiesel production from microalgae Chlorella sp. Journal of Microbiology, Biotechnology and Food Sciences, 9(4), 671-674. https://doi.org/10.15414/jmbfs.2020.9.4.671-674
  • Bhuyar, P., Sundararaju, S., Rahim, M.H.A., Ramaraj, R., Maniam, G.P., Govindan, N. (2019). Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity. Biomass Convers Biorefinery, 1–9. https://doi.org/10.1007/s13399-019-00548-5
  • Caporgno, M.P., Haberkorn, I., Böcker, L., Mathys, A. (2019). Cultivation of Chlorella protothecoides under different growth modes and its utilisation in oil/water emulsions. Bioresource Technology, 288, 121476. https://doi.org/10.1016/j.biortech.2019.121476
  • Cheirsilp, B., Torpee, S. (2012). Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. Bioresource Technology, 110(1), 510-516. https://doi.org/10.1016/j.biortech.2012.01.125
  • Choi, H.J., Lee, S.M. (2015). Biomass and oil content of microalgae under mixotrophic conditions. Environmental Engineering Research, 20(1), 25-32. https://doi.org/10.4491/eer.2014.043
  • Choi, W.J., Chae, A.N., Song, K.G., Park, J., Lee, B.C. (2019). Effect of trophic conditions on microalga growth, nutrient removal, algal organic matter, and energy storage products in Scenedesmus (Acutodesmus) obliquus KGE 17 cultivation. Bioprocess and Biosystems Engineering, 42, 1225–1234. https://doi.org/10.1007/s00449-019-02120-x
  • Chojnacka, K., Marquez-Rocha, F.J. (2004). Kinetic and stoichiometric relationships of energy and carbon metabolism in the culture on microalgae. Biotechnology, 3(2), 21–34. https://doi.org/10.3923/biotech.2004.21.34
  • Desikachary, T.V. (1959). Cyanophyta. Indian Council of Agricultural Research, New Delhi, 686 pp.
  • DuBois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(1), 350–356. https://doi.org/10.1021/ac60111a017
  • Guiry, M.D., Guiry, G.M. (2015). AlgaeBase. World-wide electronic publication. National University of Ireland, Galway [cited 2015 May 21]. Available from http://www.algaebase.org
  • Han, F., Huang, J., Li, Y., Wang, W., Wang, J., Fan, J., Shen, G. (2012). Enhancement of microalgal biomass and lipid productivities by a model of photoautotrophic culture with heterotrophic cells as seed. Bioresource Technology, 118(1), 431-437. https://doi.org/10.1016/j.biortech.2012.05.066
  • Heredia-Arroyo, T., Wei, W., Hu, B. (2010). Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides. Applied Biochemistry and Biotechnology, 162(2), 1978–1995. https://doi.org/10.1007/s12010-010-8974-4
  • Jaemin, J., Ranjna, S., Sang, J.S. (2022). The effects of acetate and glucose on carbon fixation and carbon utilization in mixotrophy of Haematococcus pluvialis. Bioresource Technology, 367, 128218. https://doi.org/10.1016/j.biortech.2022.128218
  • Katarzyna, W.C., Andrzej, N. (2004). Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures. Enzyme and Microbial Technology, 34(5), 461-465. https://doi.org/10.1016/j.enzmictec.2003.12.002
  • Kobayashi, M., Kakizono, T., Yamaguchi, K., Nishio, N., Nagai, S. (1992). Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions. Journal of Fermentation and Bioengineering, 74(1), 17–20. https://doi.org/10.1016/0922-338X(92)90261-R
  • Komárek, J. Anagnostidis, K. (2005). Süßwasserflora von Mitteleuropa, Cyanoprokaryota, 2nd Part: Oscillatoriales, 19(2), 759.
  • Lau, N.S., Matsui, M., Abdullah, A.A. (2015). Cyanobacteria: photoautotrophic microbial factories for the sustainable synthesis of industrial products. Biomedical Research. 9(1), 754934. https://doi.org/10.1155/2015/754934
  • Li, Y., Chen, Y.F., Chen, P., Min, M., Zhou, W., Martinez, B., et al. (2011). Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresource Technology, 102(2), 5138–5144. https://doi.org/10.1016/j.biortech.2011.01.091
  • Liu, X., Duan, S., Li, A., Xu, N., Cai, Z., Hu, Z. (2009). Effects of organic carbon sources on growth, photosynthesis, and respiration of Phaeodactylum tricornutum. Journal of Applied Phycology, 21(2), 246. https://doi.org/10.1007/s10811-008-9355-z
  • Marquez, F.J., Sasaki, K., Kakizono, T., Nishio, N., Nagai, S. (1993). Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions. Journal of Fermentation and Bioengineering, 76(2), 408–410. https://doi.org/10.1016/0922-338X(93)90034-6
  • Mitra, D., Leeuwen, J., Lamsal, B. (2012). Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris on industrial co-products. Algal Research, 1(1), 40–48. https://doi.org/10.1016/j.algal.2012.03.002
  • Parsons, T.T. Strickland, J.D.H. (1963). Discussion of spectrophotometric determination of marine-plant pigments, with revised equations for ascertaining chlorophylls and carotenoids. Journal of Marine Research, 21(1), 155-163.
  • Pe´rez-Pazos, J.V., Ferna´ndez-Izquierdo, P. (2011). Synthesis of neutral lipids in Chlorella sp. under different light and carbonate conditions. Science Technological Future, 4(4), 47–57. https://doi.org/10.29047/01225383.228
  • Seely, R., Duncan, J. Vidaver, E. (1972). Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide. Marine Biology, 12184-188. https://doi.org/10.1007/BF00350754
  • Singh, K.B., Kaushalendra, Rajan, J.P. (2022). Therapeu-tical and nutraceutical roles of cyanobacterial tetrapyrrole chromophore: Recent advances and future implications. front. Microbiotechnology, 13(1), 20-32. https://doi.org/10.3389/fmicb.2022.932459 Sultan, Y., Ali, M., Darwesh, O., Embaby, M., Marrez, D. (2016). Influence of nitrogen source in culture media on antimicrobial activity of Microcoleus lacustris and Oscillatoria rubescens. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(2), 1444-1452.
  • Wang, H., Xiong, H., Hui, Z., Zeng, X. (2012). Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresource Technology, 104(1), 215–220. https://doi.org/10.1016/j.biortech.2011.11.020
  • Vijayabaskar, P., Shiyamala, V. (2011). Antibacterial activities of brown marine algae (Sargassum wightii and Turbinaria ornata) from the Gulf of Mannar Biosphere Reserve. Advanced Biological Research, 5(2), 99–102.
  • Zhang, H., Wang, W., Li, Y., Yang, W., Shen, G. (2011). Mixotrophic cultivation of Botryococcus braunii. Biomass and Bioenergy, 35(2), 1710–1715. https://doi.org/10.1016/j.biombioe.2011.01.002

Effect of different trophic cultures on the amount of total carbohydrate and chlorophyll of Oscillatoria sp.

Year 2024, Volume: 7 Issue: 3, 144 - 154, 03.07.2024
https://doi.org/10.3153/AR24013

Abstract

Cyanobacteria (blue-green microalgae) is a gram-negative prokaryotic autotroph found in natural waters that plays a pivotal role in biochemical cycles. The present investigation proposed to study the potential of using different concentrations of glucose as the carbon substrate to produce microalgal biomass and biochemical components, such as photosynthetic pigments and total carbohydrates (CH) by Oscillatoria sp. The cyanobacteria were collected, and the isolated colony was found to be Oscillatoria sp., and it was grown in BG-11 medium for mass cultivation. Then, the centrifuged biomass was weighed and used to extract bioactive compounds. Oscillatoria sp. cells were cultured in three different tropic cultures (phototrophic, heterotrophic and mixotrophic) under controlled laboratory conditions with continuous light illumination or unillumination and aeration. Chl–a and total C.H. contents were also evaluated after 120 hrs. The recorded optical density of Oscillatoria was increased from 0.6798 ±0.01 at 660 nm and 0.5847 ±0.01 at 750 nm after 24 hrs to 1.2174±0.002 at 680nm and 1.0243±0.01 at 730nm at the end of 120hrs of the experiment. According to analysis results, the mean amount of Chl-a and Total C.H. of Oscillatoria sp. biomass was determined as 0.5132 µg L-1 and 3.5715 mg mL-1 under the phototrophic culture (absence of glucose), respectively. Under the mixotrophic culture (presence of light), the experimental results showed that the chl-a content was calculated as 0.1770, 0.3380 and 0.7098 µg L-1. In contrast, the total C.H. was calculated as 3.6150, 7.9129 and 11.3191 mg mL-1 in the presence of 2.5, 5 and 10 g L-1 glucose, respectively. Under the heterotrophic culture (absence of light), the results showed that the chl-a content was 0.2366, 0.2456 and 0.2346 µg L-1 while the total C.H. was 4.2969, 8.0990 and 11.5861 mg m L-1 in the presence of 2.5, 5 and 10 g L-1 glucose, respectively. The experimental results showed that the total C.H. content was increased from 3.5715 to 11.58 61 mg mL-1 in the heterotrophic (the absence of light and the presence of 10 g L-1 glucose) BG-11 culture conditions. The chlorophyll-a content was increased from 0.1770 µg L-1 to 0.7098 µg L-1 in the mixotrophic (the presence of glucose and light) BG-11 culture conditions. As a result of the experiment, it was determined that the most suitable culture in terms of total carbohydrate and growth rate was mixotrophic and heterotrophic BG-11 (10 g L-1 glucose) culture condition, and in terms of chl-a was mixotrophic culture (10 g L-1 glucose).

Ethical Statement

This study does not require ethics committee permission or any special permission.

References

  • Abeliovich, A. Weisman, D. (1978). Role of heterotrophic nutrition in growth of the alga Scenedesmus obliquus in high-rate oxidation ponds, Applied and Environmental Microbiology, 35(1), 32–37. https://doi.org/10.1128/aem.35.1.32-37.1978
  • Abad, M.J., Bedoya, L.M., Bermejo, P. (2011). Marine compounds and their antimicrobial activities. Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, 51(1), 1293–1306.
  • Ben, R.D. (2012). Photosynthetic behavior of microalgae in response to environmental factors. Applied Photosynthesis, 1(1), 23-45.
  • Bhuyar, P., Rahim, M.H.A., Yusoff, M.M., Maniam, G.P., Govindan, N. (1990). A selective microalgae strain for biodiesel production in relation to higher lipid profile. Maejo International Journal of Energy and Environmental Communication, 1(2), 8–14. https://doi.org/10.54279/mijeec.v1i1.244895
  • Bhuyar, P., Yusoff, M.M., Rahim, M.H.A., Sundararaju, S., Maniam, G.P., Govindan, N. (2020). Effect of plant hormones on the production of biomass and lipid extraction for biodiesel production from microalgae Chlorella sp. Journal of Microbiology, Biotechnology and Food Sciences, 9(4), 671-674. https://doi.org/10.15414/jmbfs.2020.9.4.671-674
  • Bhuyar, P., Sundararaju, S., Rahim, M.H.A., Ramaraj, R., Maniam, G.P., Govindan, N. (2019). Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity. Biomass Convers Biorefinery, 1–9. https://doi.org/10.1007/s13399-019-00548-5
  • Caporgno, M.P., Haberkorn, I., Böcker, L., Mathys, A. (2019). Cultivation of Chlorella protothecoides under different growth modes and its utilisation in oil/water emulsions. Bioresource Technology, 288, 121476. https://doi.org/10.1016/j.biortech.2019.121476
  • Cheirsilp, B., Torpee, S. (2012). Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. Bioresource Technology, 110(1), 510-516. https://doi.org/10.1016/j.biortech.2012.01.125
  • Choi, H.J., Lee, S.M. (2015). Biomass and oil content of microalgae under mixotrophic conditions. Environmental Engineering Research, 20(1), 25-32. https://doi.org/10.4491/eer.2014.043
  • Choi, W.J., Chae, A.N., Song, K.G., Park, J., Lee, B.C. (2019). Effect of trophic conditions on microalga growth, nutrient removal, algal organic matter, and energy storage products in Scenedesmus (Acutodesmus) obliquus KGE 17 cultivation. Bioprocess and Biosystems Engineering, 42, 1225–1234. https://doi.org/10.1007/s00449-019-02120-x
  • Chojnacka, K., Marquez-Rocha, F.J. (2004). Kinetic and stoichiometric relationships of energy and carbon metabolism in the culture on microalgae. Biotechnology, 3(2), 21–34. https://doi.org/10.3923/biotech.2004.21.34
  • Desikachary, T.V. (1959). Cyanophyta. Indian Council of Agricultural Research, New Delhi, 686 pp.
  • DuBois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(1), 350–356. https://doi.org/10.1021/ac60111a017
  • Guiry, M.D., Guiry, G.M. (2015). AlgaeBase. World-wide electronic publication. National University of Ireland, Galway [cited 2015 May 21]. Available from http://www.algaebase.org
  • Han, F., Huang, J., Li, Y., Wang, W., Wang, J., Fan, J., Shen, G. (2012). Enhancement of microalgal biomass and lipid productivities by a model of photoautotrophic culture with heterotrophic cells as seed. Bioresource Technology, 118(1), 431-437. https://doi.org/10.1016/j.biortech.2012.05.066
  • Heredia-Arroyo, T., Wei, W., Hu, B. (2010). Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides. Applied Biochemistry and Biotechnology, 162(2), 1978–1995. https://doi.org/10.1007/s12010-010-8974-4
  • Jaemin, J., Ranjna, S., Sang, J.S. (2022). The effects of acetate and glucose on carbon fixation and carbon utilization in mixotrophy of Haematococcus pluvialis. Bioresource Technology, 367, 128218. https://doi.org/10.1016/j.biortech.2022.128218
  • Katarzyna, W.C., Andrzej, N. (2004). Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures. Enzyme and Microbial Technology, 34(5), 461-465. https://doi.org/10.1016/j.enzmictec.2003.12.002
  • Kobayashi, M., Kakizono, T., Yamaguchi, K., Nishio, N., Nagai, S. (1992). Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions. Journal of Fermentation and Bioengineering, 74(1), 17–20. https://doi.org/10.1016/0922-338X(92)90261-R
  • Komárek, J. Anagnostidis, K. (2005). Süßwasserflora von Mitteleuropa, Cyanoprokaryota, 2nd Part: Oscillatoriales, 19(2), 759.
  • Lau, N.S., Matsui, M., Abdullah, A.A. (2015). Cyanobacteria: photoautotrophic microbial factories for the sustainable synthesis of industrial products. Biomedical Research. 9(1), 754934. https://doi.org/10.1155/2015/754934
  • Li, Y., Chen, Y.F., Chen, P., Min, M., Zhou, W., Martinez, B., et al. (2011). Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresource Technology, 102(2), 5138–5144. https://doi.org/10.1016/j.biortech.2011.01.091
  • Liu, X., Duan, S., Li, A., Xu, N., Cai, Z., Hu, Z. (2009). Effects of organic carbon sources on growth, photosynthesis, and respiration of Phaeodactylum tricornutum. Journal of Applied Phycology, 21(2), 246. https://doi.org/10.1007/s10811-008-9355-z
  • Marquez, F.J., Sasaki, K., Kakizono, T., Nishio, N., Nagai, S. (1993). Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions. Journal of Fermentation and Bioengineering, 76(2), 408–410. https://doi.org/10.1016/0922-338X(93)90034-6
  • Mitra, D., Leeuwen, J., Lamsal, B. (2012). Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris on industrial co-products. Algal Research, 1(1), 40–48. https://doi.org/10.1016/j.algal.2012.03.002
  • Parsons, T.T. Strickland, J.D.H. (1963). Discussion of spectrophotometric determination of marine-plant pigments, with revised equations for ascertaining chlorophylls and carotenoids. Journal of Marine Research, 21(1), 155-163.
  • Pe´rez-Pazos, J.V., Ferna´ndez-Izquierdo, P. (2011). Synthesis of neutral lipids in Chlorella sp. under different light and carbonate conditions. Science Technological Future, 4(4), 47–57. https://doi.org/10.29047/01225383.228
  • Seely, R., Duncan, J. Vidaver, E. (1972). Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide. Marine Biology, 12184-188. https://doi.org/10.1007/BF00350754
  • Singh, K.B., Kaushalendra, Rajan, J.P. (2022). Therapeu-tical and nutraceutical roles of cyanobacterial tetrapyrrole chromophore: Recent advances and future implications. front. Microbiotechnology, 13(1), 20-32. https://doi.org/10.3389/fmicb.2022.932459 Sultan, Y., Ali, M., Darwesh, O., Embaby, M., Marrez, D. (2016). Influence of nitrogen source in culture media on antimicrobial activity of Microcoleus lacustris and Oscillatoria rubescens. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(2), 1444-1452.
  • Wang, H., Xiong, H., Hui, Z., Zeng, X. (2012). Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresource Technology, 104(1), 215–220. https://doi.org/10.1016/j.biortech.2011.11.020
  • Vijayabaskar, P., Shiyamala, V. (2011). Antibacterial activities of brown marine algae (Sargassum wightii and Turbinaria ornata) from the Gulf of Mannar Biosphere Reserve. Advanced Biological Research, 5(2), 99–102.
  • Zhang, H., Wang, W., Li, Y., Yang, W., Shen, G. (2011). Mixotrophic cultivation of Botryococcus braunii. Biomass and Bioenergy, 35(2), 1710–1715. https://doi.org/10.1016/j.biombioe.2011.01.002
There are 32 citations in total.

Details

Primary Language English
Subjects Hydrobiology
Journal Section Research Articles
Authors

Tuğba Şentürk 0000-0002-9882-0079

Early Pub Date June 22, 2024
Publication Date July 3, 2024
Submission Date January 24, 2024
Acceptance Date March 22, 2024
Published in Issue Year 2024Volume: 7 Issue: 3

Cite

APA Şentürk, T. (2024). Effect of different trophic cultures on the amount of total carbohydrate and chlorophyll of Oscillatoria sp. Aquatic Research, 7(3), 144-154. https://doi.org/10.3153/AR24013

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