Research Article
Comparison of the efficacy of two phenotypic identification kits and classic PCR methods to identify Aeromonas hydrophila isolated from fish farms
Abstract
In recent years, phenotypic identification kits have been reported to give incorrect results in identifying Aeromonas species, whereas molecular identification is quite reliable. In this context, Aeromonas hydrophila strains, isolated from fish farms (9 strains), identified by polymerase chain reaction (PCR) method, and ATCC strain bacteria were used in the present study for the determination of the usability of API 20NE and Microgen GN-ID A + B panel test kits. All strains were determined as A. hydrophila in molecular methods. After phenotypic identification, a 100% accuracy rate was obtained for A. hydrophila with API 20NE. In the Microgen GN-ID A + B, these rates were 60% for the strains used in this study. Phenotypic identification for the ATCC strain in both kits was correct. This study showed that the API 20NE test kit had high validation for the rapid and correct identification of fish pathogenic A. hydrophila.
Supporting Institution
This study was funded by the Recep Tayyip Erdogan University Research Project Fund
Project Number
FYL-2019-1023
References
- Altinok, I., Kurt, I., (2003). Molecular diagnosis of fish diseases: a Review. Turkish Journal of Fisheries and Aquatic Sciences, 3(2), 131-138.
- Austin, B., Austin, D.A. (2010). Bacterial fish pathogens: diseases of farmed and wild fish, 4. Edition Springer Publishing, New York. ISBN: 978-1-4020-6068-7
- Awong-Taylor, J., Craven, K.S., Griffiths, L., Bass, C., Muscarella, M. (2007). Comparison of biochemical and molecular methods for the identification of bacterial isolates associated with failed loggerhead sea turtle eggs. Journal of Applied Microbiology, 104, 1244-1251. https://doi.org/10.1111/j.1365-2672.2007.03650.x
- Dubey S., Maiti B., Girisha S.K., Das R., Lamkhannat M., Mutoloki S., Chen S.C Karunasagar I., Evensen Ø., Munang´andu H.M. (2021). Aeromonas species obtained from different farmed aquatic species in India and Taiwan show high phenotypic relatedness despite species diversity, BMC Research Notes, 14, 313. https://doi.org/10.1186/s13104-021-05716-3
- Fernández-Bravo, A., Figueras, M.J. (2020). An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity, Microorganisms, 8, 129. https://doi.org/10.3390/microorganisms8010129
- Gülaydın, O., Özturk, C., Önalan, S., Karapınar, Z., Arabacı, M., Ekin, I.H., İlhan, Z., Gürtürk, K., İlhan, F. (2018). The investigation of the presence of some bacterial and viral agents in pearl mullet (Chalcalburnus tarichii, Pallas 1811) by Real-Time PCR and the histopathological examination. Fresenius Environmental Bulletin, 27(12), 8286-8296.
- Jin L., Chen Y., Yang W., Qiao Z., Zhang X. (2020). Complete genome sequence of fish-pathogenic Aeromonas hydrophila HX-3 and a comparative analysis: insights into virulence factors and quorum sensing, Scientific Reports, 10, 15479. https://doi.org/10.1038/s41598-020-72484-8
- Kayis, S., Er, A., Yilmaz, C., Duzgun, A., Kose, O., Kurtoglu, I.Z. (2015). Aeromonas hydrophila as a causative agent of blue sac fry syndrome in different trout species. Journal of Fish Diseases, 38(12), 1069-1071. https://doi.org/10.1111/jfd.12326
- Toobaa L., Shahzada A., Zahidb M., Muhammada R., Anamc I., Abdurd R.A., Mohammede A.A., Mater H.M (2024). Molecular characterization of Aeromonas hydrophila isolates from diseased fishes in district Kasur, Punjab, Pakistan, Brazilian Journal of Biology, 84, e254816. https://doi.org/10.1590/1519-6984.254816
- Popovic, N.T., Skukan, A.B., Strunjak-Perovic, I., Coz-Rakovac, R., Hacmanjek, M. Hunjak, B. (2004). Comparison of the API 20E and BBL Crystal E/NF identification systems for differentiating bacterial isolates from apparently healthy reared sea bass (Dicentrarchus labrax). Veterinary Research Communications, 28(2), 93-101. https://doi.org/10.1023/B:VERC.0000012113.95479.2f
- Santos, Y., Romalde, J.L., Bandin, I., Magarinos, B., Nunez, S., Barja, J.L., Toranzo, A.E. (1993). Usefulness of the API-20E system for the identification of bacterial fish pathogens. Aquaculture, 116(2-3), 111-120. https://doi.org/10.1016/0044-8486(93)90002-G
Year 2023,
Volume: 6 Issue: 2, 125 - 132, 12.04.2023
Abstract
Project Number
FYL-2019-1023
References
- Altinok, I., Kurt, I., (2003). Molecular diagnosis of fish diseases: a Review. Turkish Journal of Fisheries and Aquatic Sciences, 3(2), 131-138.
- Austin, B., Austin, D.A. (2010). Bacterial fish pathogens: diseases of farmed and wild fish, 4. Edition Springer Publishing, New York. ISBN: 978-1-4020-6068-7
- Awong-Taylor, J., Craven, K.S., Griffiths, L., Bass, C., Muscarella, M. (2007). Comparison of biochemical and molecular methods for the identification of bacterial isolates associated with failed loggerhead sea turtle eggs. Journal of Applied Microbiology, 104, 1244-1251. https://doi.org/10.1111/j.1365-2672.2007.03650.x
- Dubey S., Maiti B., Girisha S.K., Das R., Lamkhannat M., Mutoloki S., Chen S.C Karunasagar I., Evensen Ø., Munang´andu H.M. (2021). Aeromonas species obtained from different farmed aquatic species in India and Taiwan show high phenotypic relatedness despite species diversity, BMC Research Notes, 14, 313. https://doi.org/10.1186/s13104-021-05716-3
- Fernández-Bravo, A., Figueras, M.J. (2020). An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity, Microorganisms, 8, 129. https://doi.org/10.3390/microorganisms8010129
- Gülaydın, O., Özturk, C., Önalan, S., Karapınar, Z., Arabacı, M., Ekin, I.H., İlhan, Z., Gürtürk, K., İlhan, F. (2018). The investigation of the presence of some bacterial and viral agents in pearl mullet (Chalcalburnus tarichii, Pallas 1811) by Real-Time PCR and the histopathological examination. Fresenius Environmental Bulletin, 27(12), 8286-8296.
- Jin L., Chen Y., Yang W., Qiao Z., Zhang X. (2020). Complete genome sequence of fish-pathogenic Aeromonas hydrophila HX-3 and a comparative analysis: insights into virulence factors and quorum sensing, Scientific Reports, 10, 15479. https://doi.org/10.1038/s41598-020-72484-8
- Kayis, S., Er, A., Yilmaz, C., Duzgun, A., Kose, O., Kurtoglu, I.Z. (2015). Aeromonas hydrophila as a causative agent of blue sac fry syndrome in different trout species. Journal of Fish Diseases, 38(12), 1069-1071. https://doi.org/10.1111/jfd.12326
- Toobaa L., Shahzada A., Zahidb M., Muhammada R., Anamc I., Abdurd R.A., Mohammede A.A., Mater H.M (2024). Molecular characterization of Aeromonas hydrophila isolates from diseased fishes in district Kasur, Punjab, Pakistan, Brazilian Journal of Biology, 84, e254816. https://doi.org/10.1590/1519-6984.254816
- Popovic, N.T., Skukan, A.B., Strunjak-Perovic, I., Coz-Rakovac, R., Hacmanjek, M. Hunjak, B. (2004). Comparison of the API 20E and BBL Crystal E/NF identification systems for differentiating bacterial isolates from apparently healthy reared sea bass (Dicentrarchus labrax). Veterinary Research Communications, 28(2), 93-101. https://doi.org/10.1023/B:VERC.0000012113.95479.2f
- Santos, Y., Romalde, J.L., Bandin, I., Magarinos, B., Nunez, S., Barja, J.L., Toranzo, A.E. (1993). Usefulness of the API-20E system for the identification of bacterial fish pathogens. Aquaculture, 116(2-3), 111-120. https://doi.org/10.1016/0044-8486(93)90002-G
There are 11 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Hydrobiology |
| Journal Section | Research Articles |
| Authors | |
| Project Number | FYL-2019-1023 |
| Publication Date | April 12, 2023 |
| Submission Date | January 31, 2023 |
| Published in Issue | Year 2023Volume: 6 Issue: 2 |
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