Research Article

Comparison of the efficacy of two phenotypic identification kits and classic PCR methods to identify Aeromonas hydrophila isolated from fish farms

Volume: 6 Number: 2 April 12, 2023
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Aquatic Research
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Comparison of the efficacy of two phenotypic identification kits and classic PCR methods to identify Aeromonas hydrophila isolated from fish farms

Abstract

In recent years, phenotypic identification kits have been reported to give incorrect results in identifying Aeromonas species, whereas molecular identification is quite reliable. In this context, Aeromonas hydrophila strains, isolated from fish farms (9 strains), identified by polymerase chain reaction (PCR) method, and ATCC strain bacteria were used in the present study for the determination of the usability of API 20NE and Microgen GN-ID A + B panel test kits. All strains were determined as A. hydrophila in molecular methods. After phenotypic identification, a 100% accuracy rate was obtained for A. hydrophila with API 20NE. In the Microgen GN-ID A + B, these rates were 60% for the strains used in this study. Phenotypic identification for the ATCC strain in both kits was correct. This study showed that the API 20NE test kit had high validation for the rapid and correct identification of fish pathogenic A. hydrophila.

Keywords

Supporting Institution

This study was funded by the Recep Tayyip Erdogan University Research Project Fund

Project Number

FYL-2019-1023

References

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Details

Primary Language

English

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Publication Date

April 12, 2023

Submission Date

January 31, 2023

Acceptance Date

April 5, 2023

Published in Issue

Year 2023 Volume: 6 Number: 2

DOI

https://doi.org/10.3153/AR23013

IZ

https://izlik.org/JA99SP82RG

Cite

RIS / Bibtex
APA
Balcı, Ş., Er, A., İpek, Z. Z., & Kayış, Ş. (2023). Comparison of the efficacy of two phenotypic identification kits and classic PCR methods to identify Aeromonas hydrophila isolated from fish farms. Aquatic Research, 6(2), 125-132. https://doi.org/10.3153/AR23013

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