Dose-dependent cytotoxic and proliferative effects of Microcystis aeruginosa extract and its fractions on human endothelial cells

Seda Kuşoğlu Gültekin; Elif Mertoğlu Kamalı; Kaan Yılancıoğlu; Nazlı Arda

doi:10.3153/AR22011

EN

Dose-dependent cytotoxic and proliferative effects of Microcystis aeruginosa extract and its fractions on human endothelial cells

Abstract

Microcystis aeruginosa, which spreads in five continents in the world and reported in drinking water resources in 257 countries, is a dangerous microalgae for human and animal health due to its toxins. The aim of current study was to evaluate the effects of M. aeruginosa extract and its chromatographically separated fractions on human endothelial cells. In this context, crude extract was prepared from M. aeruginosa cultured in BG-11 medium, and it was fractionated by an optimized HPLC method. Algae extract and its six fractions were then analyzed for their cytotoxic effects on ECV304 using MTT assay. The results revealed that algae extract inhibited ECV304 cells by around 72%, a higher percentage than all fractions. The most toxic fraction was the first fraction, which inhibited the cells by 55%. Other fractions, except the third one, were also toxic with 35-40% inhibition percentages. Third fraction and certain doses of some fractions showed proliferative activity on ECV304 cells. These results showed that the activities of the total extract and its fractions in promoting or inhibiting cell proliferation varied depending on not only the content but also the treatment dose.

Keywords

Supporting Institution

This study was supported by the Istanbul University Research Foundation, Turkey

Project Number

FYL-2016-21655

Thanks

We would like to thank Prof. Dr. Reyhan Akçaalan Albay for her supply of cyanobacteria and Associate Prof. Dr. Belkıs Atasever Arslan for her supply of ECV304 cell line.

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Details

Primary Language

English

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Seda Kuşoğlu Gültekin
0000-0003-0674-1582
Türkiye

Elif Mertoğlu Kamalı
0000-0002-3606-4722
Türkiye

Kaan Yılancıoğlu
0000-0003-0740-5580
Türkiye

Nazlı Arda ^*
0000-0002-1043-5652
Türkiye

Publication Date

April 1, 2022

Submission Date

August 17, 2021

Acceptance Date

January 21, 2022

Published in Issue

Year 2022 Volume: 5 Number: 2

DOI

https://doi.org/10.3153/AR22011

IZ

https://izlik.org/JA65ZN82DU

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RIS / Bibtex

APA

Kuşoğlu Gültekin, S., Mertoğlu Kamalı, E., Yılancıoğlu, K., & Arda, N. (2022). Dose-dependent cytotoxic and proliferative effects of Microcystis aeruginosa extract and its fractions on human endothelial cells. Aquatic Research, 5(2), 117-128. https://doi.org/10.3153/AR22011

Öz

Dose-dependent cytotoxic and proliferative effects of Microcystis aeruginosa extract and its fractions on human endothelial cells

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite