Fluoksetin-HCl’nin Kılıçkuyruk Balıklarında (Xiphophorus hellerii) Oluşturduğu Oksidatif Hasarın Belirlenmesi

Güllü Kaymak

doi:10.3153/AR21022

TR EN

Fluoksetin-HCl’nin Kılıçkuyruk Balıklarında (Xiphophorus hellerii) Oluşturduğu Oksidatif Hasarın Belirlenmesi

Öz

Bu çalışma ile dünyada gittikçe artış gösteren sağlık sorunlarından biri olan depresyonun tedavisinde yaygın olarak kullanılan ve kardiyovasküler olarak güvenilir kabul edilen SSRI (Özgül Serotonin Geri Alım Engelleyicileri) grubu antidepresanlardan Prozac®’ın etken maddesi olan Fluoksetin Hidroklorür’ün, sucul bir organizma olan kılıçkuyruk balığı (Xiphophorus hellerii Heckel, 1848) dokularında oluşturduğu oksidatif stresin belirlenmesi amaçlanmıştır. Fluoksetin-HCl, doğada yüzey sularında 0.012 μg/L, atık sularda 0.54- 0.929 μg/L doz aralığında bulunmuştur. Bu bilgiler doğrultusunda kılıçkuyruk balıklarına 0.1 μg/L ve 1 μg/L Fluoksetin-HCl uygulaması yapılmıştır. 96 saat sonunda balıklardan kalp ve karaciğer dokuları antiseptik şartlarda disekte edilip homojenize edilmiştir. Daha sonra malondialdehit (MDA), total glutatyon (GSH) miktarları, katalaz (CAT) enzim aktivitesi, süperoksit dismutaz (SOD) enzim aktivitesi ve total protein miktarı spektrofotometrik yöntemlerle belirlenmiştir. Sonuç olarak, kalp dokuda CAT enzim aktivitesi ve MDA seviyesi azalırken, SOD enzim aktivitesi ve GSH seviyesi artmıştır. Karaciğer dokuda ise, CAT enzim aktivitesi ve GSH miktarı artarken, SOD enzim aktivitesi ve MDA seviyesi azalmıştır. Sonuç olarak bu çalışmada kontrol grubu ile yapılan karşılaştırmalar sonucu Fluoksetin-HCl’nin kılıçkuyruk balıklarında stres yolaklarını etkileyerek, stres cevabının düzenlenmesinde etkili olduğu belirlenmiştir.

Anahtar Kelimeler

References

Abreu, M.S., Koakoski, G., Ferreira, D., Oliveira, T.A., da Rosa, J.G.S., Gusso, D., Barcellos, L.J.G. (2014). Diazepam and fluoxetine decrease the stress response in zebrafish. PLoS One, 9(7), e103232. https://doi.org/10.1371/journal.pone.0103232
Aebi, H. (1984). Catalase in vitro. Methods of Enzymology, 105, 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3
Airhart, M.J., Lee, D.H., Wilson, T.D., Miller, B.E., Miller, M.N., Skalko, R.G. (2007). Movement disorders and neurochemical changes in zebrafish larvae after bath exposure to fluoxeetine (PROZAC). Neurotoxicology and Teratology, 29, 652-664. https://doi.org/10.1016/j.ntt.2007.07.005
Alsop, D., Wood, C.M. (2013). Metal and pharmaceutical mixtures: is ion loss the mechanism underlying acute toxicity and widespread additive toxicity in zebrafish?. Aquatic Toxicology, 140, 257-267. https://doi.org/10.1016/j.aquatox.2013.05.021
Balcı, B., Erkuş, A., Erkuş, F.Ş. (2010). Farmasötik bileşiklerin sucul ortamda bulunuşu ve etkileri. Research Journal of Biology Sciences, 3(2), 13-19.
Beutler, E. (1975). Reduced glutathione-GSH, u: Beutler E.(ur.) Red cell metabolism: A manual of biochemical methods. Grane and Straton, New York.
Bradford, M.M. (1976). A Rapid Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Analytical Biochemistry, 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Chen, H., Zeng, X., Mu, L., Hou, L., Yang, B., Zhao, J., Zhang, Q. (2018). Effects of acute and chronic exposures of fluoxetine on the Chinese fish, topmouth gudgeon Pseudorasbora parva. Ecotoxicology and Environmental Safety, 160, 104-113. https://doi.org/10.1016/j.ecoenv.2018.04.061

Cunha, V., Rodrigues, P., Santos, M.M., Moradas-Ferreira, P., Ferreira, M. (2016). Danio rerio embryos on Prozac Effects on the detoxification mechanism and embryo development. Aquatic Toxicology, 178, 182-189. https://doi.org/10.1016/j.aquatox.2016.08.003
Ding, J., Lu, G., Li, Y. (2016). Interactive effects of selected pharmaceutical mixtures on bioaccumulation and biochemical status in crucian carp (Carassius auratus). Chemosphere, 148, 21-31. https://doi.org/10.1016/j.chemosphere.2016.01.017
FDA-CDER (1998). Guidance for industry-Environmental assessment of human drugs and biologics applications, Revision 1.
Gutiérrez-Rodríguez, C., Morris, M.R., Dubois, N.S., de Queiroz, K. (2007). Genetic variation and phylogeography of the swordtail fish Xiphophorus cortezi (Cyprinodontiformes, Poeciliidae). Molecular Phylogenetics and Evolution, 43(1), 111-123. https://doi.org/10.1016/j.ympev.2006.10.022
Halliwell, B., Gutteridge, J.M. (2015). Free radicals in biology and medicine. Oxford University Press, USA. ISBN: 978-0-19-871747-8 https://doi.org/10.1093/acprof:oso/9780198717478.001.0001
Jin, Y., Zhang, X., Shu, L., Chen, L., Sun, L., Qian, H., Fu, Z. (2010). Oxidative stress response and gene expression with atrazine exposure in adult female zebrafish (Danio rerio). Chemosphere, 78(7), 846-852. https://doi.org/10.1016/j.chemosphere.2009.11.044
Kayım, M. H., Çağırgan, H., Güner, Y. (1999). The research of the effects of 17α-methyltestosterone on the growth of swordtail fish (Xiphophorus helleri Heckel, 1848). Journal of Fisheries and Aquatic Sciences, 16(1-2), 31-46.
Kwak, H.I., Bae, M.O., Lee, M.H., Lee, Y.S., Lee, B.J., Kang, K.S., Cho, M.H. (2001). Effects of nonylphenol, bisphenol A, and their mixture on the viviparous swordtail fish (Xiphophorus helleri). Environmental Toxicology and Chemistry: An International Journal, 20(4), 787-795. https://doi.org/10.1002/etc.5620200414
Ledwozyw, A., Michalak, J., Stepien, A., Kadziolka, A. (1986). The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta, 155, 275-283. https://doi.org/10.1016/0009-8981(86)90247-0
Li, Z., Zlabeka, V., Grabica, R., Lia, P., Machovaa, J., Veliseka, J., Randak, T. (2010). Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+–K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology, 98, 297-303. https://doi.org/10.1016/j.aquatox.2010.02.017
McCallum, E.S., Bose, A.P., Warriner, T.R., Balshine, S. (2017). An evaluation of behavioural endpoints: the pharmaceutical pollutant fluoxetine decreases aggression across multiple contexts in round goby (Neogobius melanostomus). Chemosphere, 175, 401-410. https://doi.org/10.1016/j.chemosphere.2017.02.059
Mennigen, J.A., Zamora, J.M., Chang, J.P., Trudeau, V.L. (2017). Endocrine disrupting effects of waterborne fluoxetine exposure on the reproductive axis of female goldfish, Carassius auratus. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 202, 70-78. https://doi.org/10.1016/j.cbpc.2017.08.003
Mylroie, A.A., Collins, H., Umbles, C., Kyle, J. (1986). Erythrocyte superoxide dismutase activity and other parameters of copper status in rats ingesting lead acetate. Toxicology and Applied Pharmacology, 82(3), 512-520. https://doi.org/10.1016/0041-008X(86)90286-3
Oliveira,, M.R. (2016). Fluoxetine and the mitochondria: A review of the toxicological aspects. Toxicology Letters, 256, 185-191. https://doi.org/10.1016/j.toxlet.2016.07.001
Orem, N.R., Dolph, P.J., (2002). Loss of the phospholipase C gene product induces massive endocytosis of rhodopsin and arrestin in Drosophila photoreceptors. Vision Research, 42, 497-505. https://doi.org/10.1016/S0042-6989(01)00229-2
Panlilio, J.M., Marin, S., Lobl, M.B., McDonald, M.D. (2016). Treatment with the selective serotonin reuptake inhibitor, fluoxetine, attenuates the fish hypoxia response. Scientific Reports, 6(1), 1-12. https://doi.org/10.1038/srep31148
Sayeed, I., Parvez, S., Pandey, S., Bin-Hafeez, B., Haque, R., Raisuddin, S. (2003). Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicology and Environmental Safety, 56(2), 295-301. https://doi.org/10.1016/S0147-6513(03)00009-5
Sehonova, P., Svobodova, Z., Dolezelova, P., Vosmerova, P., Faggio, C. (2018). Effects of waterborne antidepressants on non-target animals living in the aquatic environment: a review. Science of the Total Environment, 631, 789-794. https://doi.org/10.1016/j.scitotenv.2018.03.076
Sehring, I.M., Jahn, C., Weidinger, G. (2016). Zebrafish fin and heart: what's special about regeneration?. Current Opinion in Genetics & Development, 40, 48-56. https://doi.org/10.1016/j.gde.2016.05.011
Vera-Chang, M.N., Moon, T.W., Trudeau, V.L. (2019). Cortisol disruption and transgenerational alteration in the expression of stress-related genes in zebrafish larvae following fluoxetine exposure. Toxicology and Applied Pharmacology, 382, 114742. https://doi.org/10.1016/j.taap.2019.114742
Weinberger II, J., Klaper, R. (2014). Environmental concentrations of the selective serotonin reuptake inhibitor fluoxetine impact specific behaviors involved in reproduction, feeding and predator avoidance in the fish Pimephales promelas (fathead minnow). Aquatic Toxicology, 151, 77-83. https://doi.org/10.1016/j.aquatox.2013.10.012
Yan, Z., Zhang, X., Bao, X., Ling, X., Yang, H., Liu, J., Ji, Y. (2020). Influence of dissolved organic matter on the accumulation, metabolite production and multi-biological effects of environmentally relevant fluoxetine in crucian carp (Carassius auratus). Aquatic Toxicology, 226, 105581. https://doi.org/10.1016/j.aquatox.2020.105581
Yang, M., Qiu, W., Chen, J., Zhan, J., Pan, C., Lei, X., Wu, M. (2014). Growth inhibition and coordinated physiological regulation of zebrafish (Danio rerio) embryos upon sublethal exposure to antidepressant amitriptyline. Aquatic Toxicology, 151, 68-76. https://doi.org/10.1016/j.aquatox.2013.12.029
Zindler, F., Tisler, S., Loerracher, A.K., Zwiener, C., Braunbeck, T. (2020). Norfluoxetine is the only metabolite of fluoxetine in Zebrafish (Danio rerio) embryos that accumulates at environmentally relevant exposure scenarios. Environmental Science & Technology, 54(7), 4200-4209. https://doi.org/10.1021/acs.est.9b07618

Details

Primary Language

Turkish

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Güllü Kaymak ^*
0000-0001-6309-0208
Türkiye

Publication Date

July 1, 2021

Submission Date

January 18, 2021

Acceptance Date

March 16, 2021

Published in Issue

Year 1970 Volume: 4 Number: 3

DOI

https://doi.org/10.3153/AR21022

IZ

https://izlik.org/JA58YD72UT

Cite

RIS / Bibtex

APA

Kaymak, G. (2021). Fluoksetin-HCl’nin Kılıçkuyruk Balıklarında (Xiphophorus hellerii) Oluşturduğu Oksidatif Hasarın Belirlenmesi. Aquatic Research, 4(3), 286-292. https://doi.org/10.3153/AR21022

The determination of oxidative damage caused by fluoxetine hydrocloride in swordtail fish (Xiphophorus hellerii)

Fluoksetin-HCl’nin Kılıçkuyruk Balıklarında (Xiphophorus hellerii) Oluşturduğu Oksidatif Hasarın Belirlenmesi

Öz

Anahtar Kelimeler

The determination of oxidative damage caused by fluoxetine hydrocloride in swordtail fish (Xiphophorus hellerii)

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite