Removal of high concentration of nitrate and phosphate from aqueous mixotrophic solution by Chlorella vulgaris

Tuğba Şentürk; Şükran Yıldız

doi:10.3153/AR20002

EN

Removal of high concentration of nitrate and phosphate from aqueous mixotrophic solution by Chlorella vulgaris

Abstract

Microalgae exhibit large potential as an alternative to advanced biological nutrient removal in wastewater or simulated wastewater at laboratory conditions. Therefore, it is necessary to determine the optimum conditions for nutrient removal. This study investigated the total carbohydrate, chlorophyll-a, -b, carotenoid and lipid production and nutrient removal of mixotrophic microalgae (C. vulgaris) cultured in different nitrate/phosphate rich modified BG-11 medium (0-200 mg L^-1) at longer growth periods (10 days). The mean removal efficiency of NO₃-N (in nitrate source), and PO₄-P (in phosphate source) (88.29 ±0.12 and 31.06 ±0.22%, respectively) was reached in the mixotrophic culture. Under the optimum conditions (200 µmol photon m⁻²s⁻¹ 16 h photoperiod and 28% inoculum size), 63.61-99.05% of NO₃^- and 13.97-63.77% of PO₄³⁻were successfully removed. The lipid and carbohydrate productivities were 27.95 and 29.53 g L⁻¹d⁻¹, 0.2869 and 0.2435 g L^-1 d^-1 respectively, which were approximately 9-12 times higher than those in photoautotrophic condition. The BG-11 growth media containing 10 g L⁻¹ glucose and excessive amount of nutrient effect results indicate that the Chl-a, -b and carotenoid contents of C. vulgaris is higher at 100 mg L^-1 N and 50 mg L^-1 P growth media composition compared to 100% growth media composition. Thereby, the findings of this study provided an insight into the role of algal uptake of nutrients under the nutrient rich mixotrophic medium for the future algae-based treatment application.

Keywords

Bioremediation,Chlorella,Mixotrophic solution,Nutrient removal,Algal removal

Supporting Institution

Manisa Celal Bayar University Scientific Investigation Project

Project Number

FEF 2015–154

Thanks

The authors wish to express their gratitude to the Manisa Celal Bayar University Scientific Investigation Project for the funding of this work (Project No. FEF 2015–154).

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Details

Primary Language

English

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Tuğba Şentürk ^*
0000-0002-9882-0079
Türkiye

Şükran Yıldız
0000-0003-3195-2269
Türkiye

Publication Date

January 1, 2020

Submission Date

October 31, 2019

Acceptance Date

December 9, 2019

Published in Issue

Year 2020 Volume: 3 Number: 1

DOI

https://doi.org/10.3153/AR20002

IZ

https://izlik.org/JA77YY24AT

Cite

RIS / Bibtex

APA

Şentürk, T., & Yıldız, Ş. (2020). Removal of high concentration of nitrate and phosphate from aqueous mixotrophic solution by Chlorella vulgaris. Aquatic Research, 3(1), 13-23. https://doi.org/10.3153/AR20002

Öz

Removal of high concentration of nitrate and phosphate from aqueous mixotrophic solution by Chlorella vulgaris

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite