Evaluation of sucrose as carbon source in mixotrophic culture of Arthrospira platensis Gomont 1892

Zülfiye Velioğlu Tosuner; Raziye Öztürk Ürek

doi:10.3153/AR20001

EN

Evaluation of sucrose as carbon source in mixotrophic culture of Arthrospira platensis Gomont 1892

Abstract

Cyanobacteria are photosynthetic microorganisms that use CO₂ as carbon source and sunlight as energy source. Although phototrophic cultivation is widely used in cyanobacterium production, heterotrophic and mixotrophic cultivations attract attention among researchers. In this study the effect of different concentrations (0[control] - 0.25 - 2.5 - 10 - 50 mM) of sucrose on the growth of Arthrospira platensis under mixotrophic cultivation was investigated. The purpose of this study was to investigate whether A. platensis biomass production could be performed regardless of high light intensity. Biomass, chlorophyll, lipid and carbohydrate contents were determined by spectrophotometrically. Also the physicochemical properties of the produced cyanobacterium were investigated by FTIR, TGA and DSC. The highest biomass productivity was detected as 1.33 g/L/day in the medium containing 2.5 mM sucrose and the specific growth rate increased 1.32 fold as compared to phototrophic culture. Additionally, the highest lipid content (3.68 ±0.17 mg/g cell) was determined in the same medium. This suggests that A. platensis has adapted to the medium that contains low sucrose concentrations. Also, this study showed that sucrose containing medium supports lipid production.

Keywords

Sucrose,Mixotrophic culture,Phototrophic culture,Arthrospira platensis,Lipid production

Thanks

We would like to thank Assoc. Dr. Leyla Uslu for her supply of cyanobacteria.

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Details

Primary Language

English

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Zülfiye Velioğlu Tosuner ^*
0000-0001-9181-6619
Türkiye

Raziye Öztürk Ürek
0000-0002-7147-6853
Türkiye

Publication Date

January 1, 2020

Submission Date

August 7, 2019

Acceptance Date

October 6, 2019

Published in Issue

Year 2020 Volume: 3 Number: 1

DOI

https://doi.org/10.3153/AR20001

IZ

https://izlik.org/JA77HR66EU

Cite

RIS / Bibtex

APA

Velioğlu Tosuner, Z., & Öztürk Ürek, R. (2020). Evaluation of sucrose as carbon source in mixotrophic culture of Arthrospira platensis Gomont 1892. Aquatic Research, 3(1), 1-12. https://doi.org/10.3153/AR20001