PATHOGENICITY AND PATHOLOGY OF Streptococcus agalactiae IN CHALLENGED MOZAMBIQUE TILAPIA Oreochromis mossambicus (PETERS 1852) JUVENILES

Thangapalam Jawahar Abraham; Meshram Supradhnya Namdeo; Harresh Adıkesavalu; Sayani Banerjee

doi:10.3153/AR19017

EN

PATHOGENICITY AND PATHOLOGY OF Streptococcus agalactiae IN CHALLENGED MOZAMBIQUE TILAPIA Oreochromis mossambicus (PETERS 1852) JUVENILES

Abstract

Streptococcosis is one of the most important bacterial diseases of tilapia. The present study assessed the histopathological changes induced by Streptococcus agalactiae challenge in the brain, kidney, spleen, and liver of Oreochromis mossambicus. When challenged intraperitoneally at 10⁷-10⁸cells/fish, S. agalactiae strains (TKT₁ and TBT₂) caused 40-100% mortalities in O. mossambicus. The LD₅₀ values of S. agalactiae TKT₁ and TBT₂ strains were 1.60×10⁷ and 7.33×10⁷ cells/fish, respectively. Histological sections of the challenged O. mossambicus brain exhibited meningoencephalitis, marginated haemocytes, extensive haemorrhages, oedema and neurons with marginated nuclei. The kidney of challenged tilapia showed glomerulopathy, dilation of Bowman’s capsule, nephritis, haematopoietic tissue necrosis, melanization and granulomatous-like lesions. The spleen was characterized by extensive melanomacrophage aggregation, necrosis and vasodilation. The liver had dilated and ruptured blood capillary, melanization and disintegrated tissue. The intrahepatic exocrine pancreatic tissue was disintegrated. Our results demonstrated that S. agalactiae caused a systemic infection and meningoencephalitis in the Mozambique tilapia juveniles.

Keywords

Oreochromis mossambicus,Streptococcus agalactiae,Meningoencephalitis,Pathogenicity,Granulomatous-like lesions

Supporting Institution

ICAR, New Delhi

Project Number

Grant F. 10(12)/2012–EPD dated 23.03.2012

Thanks

The authors thank the Vice-Chancellor, West Bengal University of Animal and Fishery Sciences, Kolkata, India for providing necessary infrastructure facility to carry out the work.

References

Adikesavalu, H., Patra, A., Banerjee, S., Sarkar, A., Abraham, T.J. (2015). Phenotypic and molecular characterization and pathology of Flectobacillus roseus causing flectobacillosis in captive held carp Labeo rohita (Ham.) fingerlings. Aquaculture, 439, 60-65. https://doi.org/10.1016/j.aquaculture.2014.12.036
Adikesavalu, H., Banerjee, S., Patra, A., Abraham, T.J. (2017). Meningoencephalitis in farmed mono-sex Nile tilapia (Oreochromis niloticus L.) caused by Streptococcus agalactiae. Archives of Polish Fisheries, 25, 187-200. https://doi.org/10.1515/aopf-2017-0018
Al-Harbi A.H. (1996). Susceptibility of five species of tilapia to Streptococcus sp. Asian Fisheries Science, 9, 177-181.
Alsaid, M., Daud, H.H.M., Mustapha, N.M., Bejo, S.K., Abdelhadi, Y.M., Abuseliane, A.F., Hamdan, R.H. (2013). Pathological findings of experimental Streptococcus agalactiae infection in red hybrid tilapia (Oreochromis sp.). In Proceedings of the International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2013), (p. 70-73). Kuala Lumpur, Malaysia.
Amal, M.N.A., Zamri-Saad, M. (2011). Streptococcosis in tilapia (Oreochromis niloticus): a review. Pertanika Journal of Tropical Agricultural Science, 34, 195-206.
Behera, B.K. Pradhan, P.K., Swaminathan, T.R., Sood, N., Prasenjit, P., Das, A., Verma, D.K., Kumar, R., Yadav M.K., Dev, A.K., Parida, P.K., Das, B.K., Lal, K.K., Jena, J.K. (2018). Emergence of Tilapia Lake Virus associated with mortalities of farmed Nile Tilapia Oreochromis niloticus (Linnaeus 1758) in India. Aquaculture, 484, 168-174. https://doi.org/10.1016/j.aquaculture.2017.11.025
Boyd, C.E. (2004). Farm-level Issues in Aquaculture Certification: Tilapia. Report commissioned by WWF, p.29. Available at http://fisheries.tamu.edu/files/2013/09Farm-Level-Issues-in-Aquaculture-Certification-Tilapia.pdf (accessed on 19 October 2017)
El-Sayed, A.F.M. (2006). Tilapia culture. Wallingford: CAB International, p.277. https://doi.org/10.1079/9780851990149.0000

Evans, J.J., Klesius, P.H., Gilbert, P.M., Shoemaker, C.A., Al-Sarawi, M.A., Landsberg, J., Duremdez, R., Al-Marzouk, A., Al-Zenki, S. (2002). Characterization of beta-hemolytic Group B Streptococcus agalactiae in cultured gilthead seabream, Sparus auratus (L.) and wild mullet, Liza klunzingeri (Day), in Kuwait. Journal of Fish Diseases, 5, 505-513. https://doi.org/10.1046/j.1365-2761.2002.00392.x
Evans, J.J., Klesius, P.H., Shoemaker, C.A. (2006). An overview of streptococcus in warm-water fish. Aquaculture Health International, 7, 10-14.
Eyngor, M., Zamostiano, R., Tsofack, J.E.K., Berkowitz, A., Bercovier, H., Tinman, S., Lev, M., Hurvitz, A., Galeotti, M., Bacharach, E., Eldara, A. (2014). Identification of a novel RNA virus lethal to tilapia. Journal of Clinical Microbiology, 52(12), 4137-4146. https://doi.org/10.1128/JCM.00827-14
FAO (2017). Outbreaks of Tilapia lake virus (TiLV) threaten the livelihoods and food security of millions of people dependent on tilapia farming. GIEWS Special Alert No: 338 - Global. Available at: http://www.fao.org/3/a-i7326e.pdf (accessed on 20 October 2017).
FAO (2018). The State of World Fisheries and Aquaculture 2018 - Meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO.
Galhardo, L. (2010). Teleost welfare: Behavioural, cognitive and physiological aspects in Oreochromis mossambicus. PhD thesis. Porto, Portugal: Instituto De Ciências Biomédicas Abel Salazar, Universidade Do Porto, p.215
Gültepe, N., Bilen, S., Yilmaz, S., Güroy, D., Aydin, S. (2014). Effects of herbs and spice on health status of tilapia (Oreochromis mossambicus) challenged with Streptococcus iniae. Acta Veterinaria Brno, 83, 125-131. https://doi.org/10.2754/avb201483020125
Hernández, E., Figueroa, J., Iregui, C. (2009). Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study. Journal of Fish Diseases, 32, 247-252. https://doi.org/10.1111/j.1365-2761.2008.00981.x
Iregui, C., Barato, P., Rey, A., Vasquez, G., Verján, N. (2014). Epidemiology of Streptococcus agalactiae and Streptococcosis in tilapia fish. In iConcept Press Ltd (ed.), Epidemiology: Theory, Research and Practice. 1st edn. Chapter 10. Hong Kong: iConcept Press Ltd, pp.18.
Iregui, C., Comas, J., Vásquez, G.M., Verján, N. (2016). Experimental early pathogenesis of Streptococcus agalactiae infection in red tilapia Oreochromis spp. Journal of Fish Diseases, 39, 205-215. https://doi.org/10.1111/jfd.12347
Klesius, P.H., Shoemaker, C.A., Evans, J.J. (2008). Streptococcus: A worldwide fish health problem. In Proceedings of the 8th International Symposium on Tilapia in Aquaculture. (p. 83-107). Cairo, Egypt.
Li, Y.W., Liu, L., Huang, P.R., Fang, W., Luo, Z.P., Peng, H.L., Wang, X.Y., Li, A.X. (2014). Chronic streptococcosis in Nile tilapia, Oreochromis niloticus (L.) caused by Streptococcus agalactiae. Journal of Fish Diseases, 37, 757-763. https://doi.org/10.1111/jfd.12146
Mishra, A., Nam, G-H., Gim, J-A., Lee, H-E., Jo, A., Kim, H-S. (2018). Current challenges of streptococcus infection and effective molecular, cellular, and environmental control methods in aquaculture. Molecules and Cells, 41(6), 495-505.
Mukhi, S.K. (1999). Streptococcal Infection in Cultured Tilapia Oreochromis mossambicus (Peters). Master's thesis. Mumbai, India: Central Institute of Fisheries Education, p.66.
Ndong, D., Chen, Y.Y., Lin, Y.H., Vaseeharan, B., Chen, J.C. (2007). The immune response of tilapia Oreochromis mossambicus and its susceptibility to Streptococcus iniae under stress in low and high temperatures. Fish and Shellfish Immunology, 22(6), 686-694. https://doi.org/10.1016/j.fsi.2006.08.015
Pulido, E., Iregui, C., Figueroa, J., Klesius, P.H. (2004). Estreptococosis en tilapias (Oreochromis spp.) cultivadas en Colombia. Revista Aquatic, 20, 97-106.
Reed, L.J., Muench, H. (1938). A simple method of estimating fifty percent endpoints. American Journal of Epidemiology, 27(3), 493-497. https://doi.org/10.1093/oxfordjournals.aje.a118408
Roberts, R.J. (2012). Fish Pathology (4th ed.). Wiley-Blackwell, UK. p. 590. https://doi.org/10.1002/9781118222942
Sugunan, V.V. (1995). Exotic Fishes and their Role in Reservoir Fisheries in India. FAO Fisheries Technical Paper No. 345. Rome: FAO, p.423.
Trewavas, E. (1983). Tilapine fishes of the genera Sarotherodon, Oreochromis and Danakilia, London, UK: British Museum (Natural History). https://doi.org/10.5962/bhl.title.123198
Tung, M.C., Chen, S.C., Tsai, S.S. (1985). General septicemia of streptococcal infection in cage-cultured Tilapia mossambica in southern Taiwan. COA Fisheries Series No. 4, Fish Disease Research, VII: 95-105.
Wang, K.Y., Chen, D.F., Huang, L.Y., Lian, H., Wang, J., Xiao, D., Geng, Y., Yang, Z.X., Lai, W.M. (2013). Isolation and characterization of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus in China. African Journal of Microbiology Research, 7, 317-323. https://doi.org/10.5897/AJMR12.1207
Yilmaz, S., Ergün, S., Soytaş, N. (2013). Herbal supplements are useful for preventing streptococcal disease during first-feeding of tilapia fry, Oreochromis mossambicus. Israeli Journal of Aquaculture Bamidgeh, 65, 833.
Zamri-Saad, M., Amal, M.N.A., Siti-Zahrah, A. (2010). Pathological changes in red tilapias (Oreochromis spp.) naturally infected by Streptococcus agalactiae. Journal of Comparative Pathology, 143, 227-229. https://doi.org/10.1016/j.jcpa.2010.01.020
Zamri-Saad, M., Amal, M.N.A., Siti-Zahrah, A., Zulkafli, A.R. (2014). Control and prevention of streptococcosis in cultured tilapia in Malaysia: A review. Pertanika Journal of Tropical Agricultural Science, 37(4), 389-410.
Zlotkin, A., Hershko, H., Eldar, A. (1998). Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Applied and Environmental Microbiology, 64, 4065-4067.

Details

Primary Language

English

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Thangapalam Jawahar Abraham ^*
0000-0003-0581-1307
India

Meshram Supradhnya Namdeo
0000-0002-6046-9703
India

Harresh Adıkesavalu
0000-0002-2258-1470
India

Sayani Banerjee
0000-0001-6527-4481
India

Publication Date

October 1, 2019

Submission Date

August 5, 2019

Acceptance Date

September 20, 2019

Published in Issue

Year 1970 Volume: 2 Number: 4

DOI

https://doi.org/10.3153/AR19017

IZ

https://izlik.org/JA88ZM37YF

Cite

RIS / Bibtex

APA

Abraham, T. J., Namdeo, M. S., Adıkesavalu, H., & Banerjee, S. (2019). PATHOGENICITY AND PATHOLOGY OF Streptococcus agalactiae IN CHALLENGED MOZAMBIQUE TILAPIA Oreochromis mossambicus (PETERS 1852) JUVENILES. Aquatic Research, 2(4), 182-190. https://doi.org/10.3153/AR19017

Öz

PATHOGENICITY AND PATHOLOGY OF Streptococcus agalactiae IN CHALLENGED MOZAMBIQUE TILAPIA Oreochromis mossambicus (PETERS 1852) JUVENILES

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

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Submission Date

Acceptance Date

Published in Issue

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