Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish

Utku Duran; Esen Kulaç Polat; Sena Çenesiz; Büşra Erdoğan; Recai Aci

doi:10.3153/AR26016

EN

Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish

Abstract

Turkish salmon (Oncorhynchus mykiss) has significant economic importance in Turkish aquaculture and export and is a key species supporting sustainable production, particularly in the Black Sea Region. Live fish transfer between facilities is an unavoidable step in the production cycle and may induce physiological and biochemical stress responses. Understanding transfer-related stress is essential for maintaining fish welfare and improving production efficiency. In this study, the effects of live transfer on selected biochemical parameters, serum electrolytes, and oxidative stress indicators were investigated in Turkish salmon. A total of 30 healthy fish (400±50 g, 12 months old) were divided into a pre-transfer control group and a post-transfer group following road transportation. Serum glucose, urea, uric acid, AST, ALP, LDH, Fe, total protein, and cortisol levels were analyzed. Significant increases were detected in glucose, AST, iron, and LDH levels in the transfer group compared to the control group (p<0.001), while cortisol levels significantly decreased (p<0.001). In addition, total oxidant status (TOS) and oxidative stress index (OSI) values significantly increased (p<0.05). These findings indicate that live transfer significantly affects metabolic, enzymatic, and oxidative stress responses, and optimization of transfer protocols is critical for fish welfare and production success.

Keywords

Ethical Statement

All experimental procedures were conducted in accordance with national and institutional guidelines for the care and use of experimental animals. Ethical approval for this study was obtained from the Local Ethics Committee for Aquatic Vertebrate Animal Experiments of the Central Fisheries Research Institute, Ministry of Agriculture and Forestry (Decision No: 325.04.02-27, Date: 26.01.2026).

Thanks

The authors would like to thank İlker Yıldırım, General Manager of Yomra Su Ürünleri Yet. Tur. Tic. Ltd. Şti., for his support during the sampling and field study process. The authors also thank the farm staff for their technical assistance and cooperation during the study.

References

Barton, B.A. (2002). Stress in Fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42(3), 517–525. https://doi.org/10.1093/icb/42.3.517
Berka, R. (1986). (FAO Fisheries Technical Paper No. 48). Rome, Italy: Food and Agriculture Organization of the United Nations. ISBN 92-5-102380-8 http://www.flyingsharks.eu/literature/fao_iata/Transport_of_live_fish_FAO.pdf (accessed 26.01.2026).
Çakmak, E., Çankırılıgil, E.C., Özel, O.T. (2018). The fifth culture generation of Black Sea Trout (Salmo trutta labrax): Culture characteristics, meat yield, and proximate composition. Ege Journal of Fisheries & Aquatic Sciences, 35(1),103–110. https://doi.org/10.12714/egejfas.2018.35.1.16
Çakmak, E., Özel, O.T., Batır, E., Evin, D. (2024). A new approach in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) industry: “Turkish Salmon” production and marketing trends. Ege Journal of Fisheries & Aquatic Sciences, 41(1), 69–81. https://doi.org/10.12714/egejfas.41.1.10
Carriquiriborde, P., Handy, R.D., Davies, S.J. (2004). Physiological modulation of iron metabolism in rainbow trout (Oncorhynchus mykiss) fed low and high iron diets. Journal of Experimental Biology, 207(1), 75–86. https://doi.org/10.1242/jeb.00712
Chowdhury, S., Saikia, S.K. (2020). Oxidative stress in fish: A review. Journal of Scientific Research, 12(1). https://doi.org/10.3329/jsr.v12i1.41716
Devkota, H.R., Jha, D.K., Joshi, T.P., Shrestha, S., Bhandari, M.P. (2023). Enhancing the survival rate in live fish transport by utilizing nanobubble technology. Nepalese Journal of Aquaculture and Fisheries, 10, 33–42. https://doi.org/10.3126/njaf.v10i1.78789
Duran, U., Çenesiz, S. (2023). Biochemical effects of live fish transports: A comprehensive review. Proceedings International Conference on Fisheries and Aquaculture, 10(1), 76-90. https://doi.org/10.1750123861282.2023.10107

Duran, U., Çenesiz, S. (2025). The effect of different oxygen concentrations on oxidative stress and some biochemical parameters in the transfer of adult rainbow trout (Oncorhynchus mykiss). BMC Veterinary Research, 21(1), 302. https://doi.org/10.1186/s12917-025-04770-4
Erol, S., Erüz, C. (2023). Karadeniz’in su ürünleri yetiştiriciliği sektörü açısından potansiyeli ve Türk somonu ihracatının finansal ve ekonomik analizi. Uluslararası Ekonomi ve Yenilik Dergisi, 10(1), 47–64. https://doi.org/10.20979/ueyd.1370684
Esposito, G., Bergagna, S., Colussi, S., Shahin, K., Rosa, R., Volpatti, D., Faggio, C., Mossotto, C., Gabetti, A., Maganza, A., et al. (2024). Changes in blood serum parameters in farmed rainbow trout (Oncorhynchus mykiss) during a piscine lactococcosis outbreak. Journal of Fish Diseases, 47(10), e13994. https://doi.org/10.1111/jfd.13994
FAO (2025). National Aquaculture Sector Overview - Turkey. Türkiye. Text by Deniz, H.. In: Fisheries and Aquaculture. http://www.fao.org/fishery/countrysector/TR/en?status=published (accessed 26.01.2026).
Fazio, F., Saoca, C., Capillo, G., Iaria, C., Panzera, M., Piccione, G., Spanò, N. (2021). Intra-variability of some biochemical parameters and serum electrolytes in rainbow trout (Walbaum, 1792) bred using a flow-through system. Heliyon, 7(2). https://doi.org/10.1016/j.heliyon.2021.e06361
Gao, P., Chen, R., Lin, S., Yu, H., Zhang, X. (2025). Effects of MS-222 on the physiological and biochemical response, histology, gut bacteria, and flesh quality in largemouth bass (Micropterus salmoides). Fish Physiology and Biochemistry, 51, 176. https://doi.org/10.1007/s10695-025-01591-y
van Berkum, E. (2017). Long distance fish transport with fine bubbles, less fish stress and more sustainable. Acniti. https://www.acniti.com/blog/long-distance-fish-transport-with-fine-bubbles/ (accessed 26.01.2026).
Kamalam, J.B.S., Patiyal, R., Manchi, R., Mir, J., Singh, A.K. (2017). Prolonged transport of rainbow trout fingerlings in plastic bags: Optimization of hauling conditions based on survival and water chemistry. Aquaculture, 480. https://doi.org/10.1016/j.aquaculture.2017.08.012
Kubilay, A., Uluköy, G. (2002). The effects of acute stress on rainbow trout (Oncorhynchus mykiss). Turkish Journal of Zoology, 26(2),249–254.
Madaro, A., Nilsson, J., Whatmore, P., Roh, H., Grove, S., Stien, L.H., Olsen, R.E. (2023). Acute stress response on Atlantic salmon: a time-course study of the effects on plasma metabolites, mucus cortisol levels, and head kidney transcriptome profile. Fish Physiology and Biochemistry, 49(1),97–116. https://doi.org/10.1007/s10695-022-01163-4
Martínez-Porchas, M., Martínez-Córdova, L.R., Ramos-Enríquez, R. (2009). Cortisol and glucose: reliable indicators of fish stress?. Pan-American Journal of Aquatic Sciences, 158–178.
Mazeaud, M.M., Mazeaud, F., Donaldson, E.M. (1977). Primary and secondary effects of stress in fish: some new data with a general review. Transactions of the American Fisheries Society, 106(3), 201–212. https://doi.org/10.1577/1548-8659(1977)106<201:PASEOS>2.0.CO;2
Midtlyng, P.J., Lillehaug, A. (1998). Growth of Atlantic salmon Salmo salar after intraperitoneal administration of vaccines containing adjuvants. Diseases of Aquatic Organisms, 32(2), 91–97. https://doi.org/10.3354/dao032091
Mommsen, T.P., Vijayan, M.M., Moon, T.W. (1999). Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation. Reviews in Fish Biology and Fisheries, 9, 211–268. https://doi.org/10.1023/A:1008924418720
Naderi, M., Keyvanshokooh, S., Ghaedi, A., Salati, A.P. (2018). Effect of acute crowding stress on rainbow trout (Oncorhynchus mykiss): a proteomics study. Aquaculture, 495,106–114. https://doi.org/10.1016/j.aquaculture.2018.05.038
Panase, P., Saenpheti, S., Saenphet, K. (2018). Biochemical and physiological responses of Nile tilapia Oreochromis niloticus Lin subjected to cold shock of water temperature. Aquaculture Reports, 11, 17–23. https://doi.org/10.1016/j.aqrep.2018.05.005
van Raaij, M.T.M., Pit, D.S.S., Balm, P.H.M., Steffens, A.B., van den Thillart, G.E.E.J.M. (1996). Behavioral strategy and the physiological stress response in rainbow trout exposed to severe hypoxia. Hormones and Behavior, 30(1), 85–92. https://doi.org/10.1006/hbeh.1996.0012
Refaey, M.M., Li, D. (2018). Transport stress changes blood biochemistry, antioxidant defense system, and hepatic HSPs mRNA expressions of channel catfish Ictalurus punctatus. Frontiers in Physiology, 9,1–11. https://doi.org/10.3389/fphys.2018.01628
Ross, L.G., & Ross, B. (2008). Anaesthetic and sedative techniques for aquatic animals. Wiley-Blackwell, Ames, IA, USA. ISBN 9781405149389. https://doi.org/10.1002/9781444302264
Rusco, G., Forte, L., Prete, A., Maggiolino, A., De Palo, P., Casalino, E., Centoducati, G. (2026). Effects of a novel mixture of natural additives on mucosal and blood biomarkers in European sea bass (Dicentrarchus labrax) fry under transport stress. Italian Journal of Animal Science, 25(1),1–13. https://doi.org/10.1080/1828051X.2025.2610076
Schreck, C.B., Tort, L., Farrell, A.P., & Brauner, C.J. (2016). Biology of Stress in Fish (Fish Physiology, Vol. 35). Academic Press, London, UK. ISBN 978-0-12-802728-8
Shabani, F., Erikson, U., Beli, E., Rexhepi, A. (2016). Live transport of rainbow trout (Oncorhynchus mykiss) and subsequent live storage in market: Water quality, stress and welfare considerations. Aquaculture, 453, 110–115. https://doi.org/10.1016/j.aquaculture.2015.11.040
Suarez-Bregua, P., Guerreiro, P.M., Rotllant, J. (2018). Stress, glucocorticoids and bone: A review from mammals and fish. Frontiers in Endocrinology, 9, 526. https://doi.org/10.3389/fendo.2018.00526
Suárez, M.D., Trenzado, C.E., García-Gallego, M., Furné, M., García-Mesa, S., Domezain, A., Alba, I., Sanz, A. (2015). Interaction of dietary energy levels and culture density on growth performance and metabolic and oxidative status of rainbow trout (Oncorhynchus mykiss). Aquacultural Engineering, 67, 59–66. https://doi.org/10.1016/j.aquaeng.2015.06.001
Tacchi, L., Lowrey, L., Musharrafieh, R., Crossey, K., Larragoite, E.T., Salinas, I. (2010). Effects of transportation stress and addition of salt to transport water on the skin mucosal homeostasis of rainbow trout (Oncorhynchus mykiss). Aquaculture, 23(1), 1–7. https://doi.org/10.1016/j.aquaculture.2014.09.027
Tuna Keleştemur, G., Özdemir, Y. (2008). Nakil işlemlerinin Gökkuşağı Alabalığı (Oncorhynchus mykiss, W., 1792)’nın kortizol ve glikoz düzeyleri üzerine etkisi. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 4(1), 1–2.
Tuna Keleştemur, G., Özdemir, Y. (2010). Nakil işleminin gökkuşağı alabalıkları (Oncorhynchus mykiss, W. 1792)'nın bazı kan parametre değerleri üzerine etkileri. Süleyman Demirel University Faculty of Arts and Science Journal of Science, 5(2), 187-193.
Vijayan, M.M., Moon, T.W. (1992). Acute handling stress alters hepatic glycogen metabolism in food-deprived rainbow trout (Oncorhynchus mykiss). Canadian Journal of Fisheries and Aquatic Sciences, 49(11), 2260–2266. https://doi.org/10.1139/f92-247
Vijayan, M.M., Pereira, C., Moon, T.W. (1994). Hormonal stimulation of hepatocyte metabolism in rainbow trout following an acute handling stress. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 108(3), 321–329. https://doi.org/10.1016/0742-8413(94)00024-5
Wedemeyer, G. (1997). Effects of rearing conditions on the Health and physiological quality of fish in Intensive culture, chap. of Fish stress and health in aquaculture, p. 35-72.
Wedemeyer, G., Chatterton, K. (1970). Some blood chemistry values for the Rainbow Trout (Salmo gairdneri). Journal of the Fisheries Board of Canada, 27(6), 1162–1164. https://doi.org/10.1139/f70-135
Wendelaar Bonga, S.E. (1997). The stress response in fish. Physiological Reviews, 77(3), 591–625. https://doi.org/10.1152/physrev.1997.77.3.591
Wischhusen, P., Larroquet, L., Durand, T., Oger, C., Galano, J-M., Rocher, A., Vigor, C., Antony Jesu Prabhu, P., Véron, V., Briens, M., et al. (2020). Oxidative stress and antioxidant response in rainbow trout fry exposed to acute hypoxia are affected by selenium nutrition of parents and during the first exogenous feeding. Free Radical Biology and Medicine, 155, 99–113. https://doi.org/10.1016/j.freeradbiomed.2020.05.006
Witeska, M., Kondera, E., Ługowska, K., Bojarski, B. (2022). Hematological methods in fish – Not only for beginners. Aquaculture, 547. https://doi.org/10.1016/j.aquaculture.2021.737498
Yıldırım, Ö., Çantaş, İ.B. (2022). Türkiye’de Gökkuşağı alabalığı yetiştiriciliğinin üretim ve ekonomik göstergelerinin incelenmesi. Acta Aquatica Turcica, 18(4), 461–474. https://doi.org/10.22392/actaquatr.1101098

Details

Primary Language

English

Subjects

Analytical Biochemistry, Fisheries Technologies, Aquaculture

Journal Section

Research Article

Authors

Utku Duran ^*
0000-0002-0002-8893
Türkiye

Esen Kulaç Polat
0000-0002-7981-073X
Türkiye

Sena Çenesiz
0000-0002-3544-503X
Türkiye

Büşra Erdoğan
0000-0002-5245-478X
Türkiye

Recai Aci
0000-0002-1517-3356
Türkiye

Early Pub Date

June 27, 2026

Publication Date

July 1, 2026

Submission Date

February 13, 2026

Acceptance Date

April 2, 2026

Published in Issue

Year 2026 Volume: 9 Number: 3

DOI

https://doi.org/10.3153/AR26016

IZ

https://izlik.org/JA63BT95LK

Cite

RIS / Bibtex

APA

Duran, U., Kulaç Polat, E., Çenesiz, S., Erdoğan, B., & Aci, R. (2026). Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish. Aquatic Research, 9(3), 191-201. https://doi.org/10.3153/AR26016

AMA

1.Duran U, Kulaç Polat E, Çenesiz S, Erdoğan B, Aci R. Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish. Aquat Res. 2026;9(3):191-201. doi:10.3153/AR26016

Chicago

Duran, Utku, Esen Kulaç Polat, Sena Çenesiz, Büşra Erdoğan, and Recai Aci. 2026. “Effects of Live Transfer on Serum Biochemistry and Oxidative Stress Parameters in the Turkish Salmon Candidate Fish”. Aquatic Research 9 (3): 191-201. https://doi.org/10.3153/AR26016.

EndNote

Duran U, Kulaç Polat E, Çenesiz S, Erdoğan B, Aci R (July 1, 2026) Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish. Aquatic Research 9 3 191–201.

IEEE

[1]U. Duran, E. Kulaç Polat, S. Çenesiz, B. Erdoğan, and R. Aci, “Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish”, Aquat Res, vol. 9, no. 3, pp. 191–201, July 2026, doi: 10.3153/AR26016.

ISNAD

Duran, Utku - Kulaç Polat, Esen - Çenesiz, Sena - Erdoğan, Büşra - Aci, Recai. “Effects of Live Transfer on Serum Biochemistry and Oxidative Stress Parameters in the Turkish Salmon Candidate Fish”. Aquatic Research 9/3 (July 1, 2026): 191-201. https://doi.org/10.3153/AR26016.

JAMA

1.Duran U, Kulaç Polat E, Çenesiz S, Erdoğan B, Aci R. Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish. Aquat Res. 2026;9:191–201.

MLA

Duran, Utku, et al. “Effects of Live Transfer on Serum Biochemistry and Oxidative Stress Parameters in the Turkish Salmon Candidate Fish”. Aquatic Research, vol. 9, no. 3, July 2026, pp. 191-0, doi:10.3153/AR26016.

Vancouver

1.Utku Duran, Esen Kulaç Polat, Sena Çenesiz, Büşra Erdoğan, Recai Aci. Effects of live transfer on serum biochemistry and oxidative stress parameters in the Turkish Salmon candidate fish. Aquat Res. 2026 Jul. 1;9(3):191-20. doi:10.3153/AR26016