Research Article
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Year 2026, Issue: Advanced Online Publication, 129 - 147, 30.03.2026

Özay Köse

https://doi.org/10.3153/AR26012
https://izlik.org/JA44YS64DW

Abstract

Project Number

FBA-2023-15

References

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Functional effects of quercetin via the haematology–gut axis: Resistance to Lactococcus petauri in Oncorhynchus mykiss

Year 2026, Issue: Advanced Online Publication, 129 - 147, 30.03.2026

Özay Köse

https://doi.org/10.3153/AR26012
https://izlik.org/JA44YS64DW

Abstract

This study evaluated the effects of low-fat (LFD), high-fat (HFD), and quercetin-supplemented high-fat (HFD+Q; 0.2 g/kg) diets on the haematological responses, intestinal histomorphology/histopathology, and resistance to Lactococcus petauri infection in rainbow trout (Oncorhynchus mykiss). A total of 270 fish (initial mean weight: 38.37 g) were randomly distributed into three dietary groups with three replicates per treatment (30 fish per tank) and fed the experimental diets for eight weeks. At the end of the trial, haematological parameters were determined using an automated analyser; histomorphometric measurements (muscle layers, villus parameters, and goblet cell counts) and histopathological evaluations were performed on intestinal samples. Furthermore, an intraperitoneal challenge with L. petauri was conducted to evaluate disease resistance. The HFD group exhibited significant suppression of leukocyte profiles (WBC, LYM, MID, GRAN) and a downward trend in HGB/HCT and erythrocyte indices. This group also displayed intestinal alterations consistent with muscular layer irregularities, submucosal oedema, and loss of goblet cells. In contrast, the HFD+Q group maintained more balanced haematological parameters and intestinal barrier indicators, contributing to the recovery of mucosal defence by increasing goblet cell counts compared to the HFD group. In the challenge trial, the survival probability was significantly higher in the HFD+Q group. In conclusion, quercetin is a functional feed additive with the potential to enhance resistance to L. petauri infection by supporting haematological homeostasis and intestinal integrity under high-fat feeding conditions. Further immunological and molecular studies are recommended to elucidate the mechanism of action and optimal dosage.

Keywords

Oncorhynchus mykiss Lactococcus petauri Quercetin High-fat diet Haematology Intestinal histology

Ethical Statement

The animal study was approved by Recep Tayyip Erdoğan University Experimental Animals Ethics Committee (decision number: 2023/30, date: June 13, 2023). The study was conducted in accordance with the local legislation and institutional requirements.

Supporting Institution

Scientific Research Projects Coordination Unit of Recep Tayyip Erdogan University

Project Number

FBA-2023-15

Thanks

The author, Dr. Ö.K., would like to thank Dr. Salih KUMRU for proofreading the manuscript in its original language. Special thanks are also extended to Dr. Akir ER for his assistance with the challenge test protocol and to Yusuf Demir, our Data Collection Assistant, for his assistance with record keeping during data collection

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  • Zahran, E., Risha, E., Awadin, W., & Palić, D. (2018). Acute exposure to chlorpyrifos induces reversible changes in health parameters of Nile tilapia (Oreochromis niloticus). Aquatic Toxicology, 197(February), 47–59. https://doi.org/10.1016/j.aquatox.2018.02.001
  • Zhang, Y., Xiao, C., & Zhu, F. (2021). Effects of dietary quercetin on the innate immune response and resistance to white spot syndrome virus in Procambarus clarkii. Fish & Shellfish Immunology, 118(July), 205–212. https://doi.org/10.1016/j.fsi.2021.09.012
  • Zhou, J., Feng, P., Li, Y., Ji, H., & Gisbert, E. (2024). Effects of dietary lipid levels on lipid accumulation and health status of adult Onychostoma macrolepis. Aquaculture and Fisheries, 9(5), 795–803. https://doi.org/10.1016/j.aaf.2023.07.008
There are 76 citations in total.

Details

Primary Language English
Subjects Fish Physiology and Genetics
Journal Section Research Article
Authors

Özay Köse 0000-0002-3565-160X

Project Number FBA-2023-15
Submission Date December 26, 2025
Acceptance Date February 9, 2026
Early Pub Date March 30, 2026
Publication Date March 30, 2026
DOI https://doi.org/10.3153/AR26012
IZ https://izlik.org/JA44YS64DW
Published in Issue Year 2026 Issue: Advanced Online Publication

Cite

APA Köse, Ö. (2026). Functional effects of quercetin via the haematology–gut axis: Resistance to Lactococcus petauri in Oncorhynchus mykiss. Aquatic Research, Advanced Online Publication, 129-147. https://doi.org/10.3153/AR26012

Aim & Scope

   The journal “AQUATIC RESEARCH” is an international, scientific, open access journal published in accordance with the principles of independent, impartial and double-blind refereeing. The journal is published quarterly in January, April, July, and October to contribute to the literature by publishing articles at the highest scientific level in all fields of aquatic sciences. The publication language of the journal is English or Turkish, and it has been published since 2018.

     The journal publishes original research and review articles prepared in accordance with ethical rules.

The publication of the "AQUATIC RESEARCH" journal are not limited to these; Aquatic Biology, Aquatic Ecology, Aquatic Environment and Pollutants, Aquaculture, Conservation and Management of Aquatic Source, Economics and Managements of Fisheries, Fish Diseases and Health, Fisheries Resources and Management, Genetics of Aquatic Organisms, Limnology, Maritime Sciences, Marine Accidents, Marine Navigation and Safety, Marine and Coastal Ecology, Oceanography, Seafood Processing and Quality Control, Seafood Safety Systems, Sustainability in Marine and Freshwater Systems etc. covers topics. The target audience of the journal consists of experts and professionals working and interested in all disciplines of aquatic sciences.

Processing and publication are free of charge with the journal. There is no article processing charges or submission fees for any submitted or accepted articles. Statements or opinions expressed in articles published in the journal reflect the views of the author(s), not the views of the editors, editorial board and/or publisher; The editors, editorial board, and publisher do not accept any responsibility or liability for such materials.

To guarantee that all papers published in the journal are maintained and permanently accessible, articles are stored in Dergipark (https://dergipark.org.tr) which serves as a national archival web site and at the same time permits LOCKSS to collect, preserve, and serve the content. All expenses of the journal are covered by the ScientificWebJournals web portal.

In addition, authors are encouraged to self-archive the final PDF version of their articles in open electronic archives that comply with Open Archive Initiative (https://www.openarchives.org/) standards. Authors should provide a link from the stored version to the journal website URL.

Editor in Chief: Prof. Dr. Özkan Özden

Address: Istanbul University Faculty of Aquatic Sciences, Kalenderhane Mah. 16 Mart Şehitleri Cad. No:2 34134 Vezneciler Fatih/Istanbul, Türkiye

E-mail: ozden@istanbul.edu.tr 


Header photos (web and others) from Ferhan Coşkun, Türkiye

E.mail: fcoskun@gmail.com

Instagram: instagram.com/exultsoul

Article evaluation and submission rules for reviewers and authors are given in the link below.


https://dergipark.org.tr/tr/download/journal-file/26282 

The journal “AQUATIC RESEARCH” adheres to the highest standards of publishing ethics guided by the International Committee of Medical Journal Editors (ICMJE), World Association of Medical Editors (WAME), Council of Science Editors (CSE), Committee on Publication Ethics (COPE), European Association of Science Editors (EASE), Open Access Scholarly and Publishers Association (OASPA), and  Directory of Open Access Journals (DOAJ).

Authors must comply with the IUCN Policy Statement on Research Involv-ing Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora for research involving plants.

Manuscripts submitted should align with the journal's purpose and scope and must be original, unpublished, and not under consideration elsewhere. Changes to authorship (name, order, additions) require written consent from all declared authors.

The following actions constitute unethical behavior and result in rejection or retraction: plagiarism, duplication, false authorship, data fabrication, slicing, copyright infringement, and undisclosed conflicts of interest. 

Journal Responsibilities
The "AQUATIC RESEARCH" journal is committed to preventing publication malpractice. Submitted works must represent original contributions free from plagiarism. Authors must disclose actual or potential conflicts of interest. The journal ensures objective and fair double-blind peer-review to prevent conflicts between editorial staff, reviewers, and authors
The journal provides an open platform for public discussion requiring registration for accountability. Reviewer identities remain confidential unless explicitly disclosed to the editors.
Publication Decisions
The editor of the "AQUATIC RESEARCH" journals is responsible for deciding which of the articles submitted to the journal should be published. The editor may be guided by the policies of the journal's editorial board and constrained by such legal requirements as shall then be in force regarding libel, copyright infringement and plagiarism. The editor may confer with other editors or reviewers in making this decision.
The journal submission and publication process involves manuscript submission, ethical checks, double-blind peer review, editorial decision-making (including potential conflict resolution), typesetting, author review, DOI assignment, online publication, and archiving, as detailed in the workflow diagram below.
34014
Fair Play
An editor at any time evaluate manuscripts for their intellectual content without regarding race, gender, sexual orientation, religious belief, ethnic origin, citizenship, or political philosophy of the authors.
Confidentiality
The editor and any editorial staff must not disclose any information about a submitted manuscript to anyone other than the corresponding author, reviewers, potential reviewers, other editorial advisers, and the publisher, as appropriate.
Disclosure and Conflicts of Interest
Unpublished materials disclosed in a submitted manuscript must not be used in an editor's own research without the express written consent of the author.
Research Ethics
An approval of research protocols by the Ethics Committee in accordance with international agreements (World Medical Association Declaration of Helsinki “Ethical Principles for Medical Research Involving Human Subjects,” amended in October 2013, www.wma.net) is required for experimental, clinical, and drug studies. If required, ethics committee reports or an equivalent official document will be requested from the authors.
For manuscripts concerning experimental research on humans, a statement should be included that shows the written informed consent of patients and volunteers was obtained following a detailed explanation of the procedures that they may undergo. Information on patient consent, the name of the ethics committee, and the ethics committee approval number should also be stated in the Materials and Methods section of the manuscript. It is the authors’ responsibility to carefully protect the patients’ anonymity. For photographs that may reveal the identity of the patients, signed releases of the patient or of their legal representative should be enclosed.
“AQUATIC RESEARCH” journal requires experimental research studies on vertebrates or any regulated invertebrates to comply with relevant institutional, national and/or international guidelines. The journal supports the principles of the Basel Declaration (https://animalresearchtomorrow.org/en) and the guidelines published by the International Council for Laboratory Animal Science (ICLAS) (https://iclas.org/). Authors are advised to clearly state their compliance with relevant guidelines.
This journal advises authors to comply with IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora for research involving plants.
Use of Artificial Intelligence (AI) Tools
Our journal adheres to international standards of publication ethics and requires complete transparency regarding the use of artificial intelligence (AI)-based tools (e.g., ChatGPT, Copilot, etc.) during manuscript preparation.
Disclosure Requirement: Authors must disclose the use of any AI tool in the manuscript, specifying the name of the tool, the stage(s) at which it was used, and its purpose. This text should be included in the "Disclosure". Authors must affirm that all scientific content is their own and that they retain full responsibility for any material generated with the assistance of AI tools.
Content Limitation: The use of AI tools must remain supplementary. If the proportion of AI-generated text content exceeds 20% of the total manuscript, the submission will be considered ineligible for review and rejected on ethical grounds.
Responsibility: AI tools cannot be listed as authors. All named authors are solely accountable for the content of the submission, including any material produced with AI assistance. Failure to disclose the use of AI tools or excessive reliance on them will be considered a breach of publication ethics.
Plagiarism
Submitted manuscripts that pass preflight are scanned for plagiarism using iThenticate software. Authors will be notified if plagiarism/self-plagiarism is detected. Editors can resubmit the article for any peer-review or similarity check during production, if necessary. High similarity scores can cause an article to be rejected before or even after it is accepted. Depending on the type of article and the percentage of similarity scores from each article, the overall similarity score is generally expected to be less than 20%.
Double-Blind Referee Evaluation
After the plagiarism check, the appropriate ones are evaluated by the editors in terms of originality, methodology, importance of the subject and suitability to the scope of the journal. The editor directs the submitted articles to a fair double-blind peer-review (submits the articles that comply with official rules to at least two national/international referees for evaluation) and gives approval by managing the processes for publication after they are modified by the authors in accordance with the referees' rules.
Open Access Statement
The journal is an open access journal and all its content is freely available to the user or institution. Users are permitted to read, download, copy, print, search or link the full texts of articles in this journal without prior permission from the publisher or author. This conforms to Budapest Open Access Initiative (BOAI) 's definition of open access.
Open access articles in the journal are licensed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) license.
Article Processing Fee
All journal processes are free of charge. No article processing fee, submission fee or publication fee is charged for submitted or accepted articles.
Copyright Notice
Authors publishing with the journal retain copyright to their work licensed under a Creative Commons Attribution 4.0 International license (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/), and Publisher retains the exclusive right to publish the work. The CC BY 4.0 license permits unlimited distribution and reproduction in any medium, provided that the original work is properly cited.
The copyright of any open access article in the "AQUATIC RESEARCH" journal published on the "ScientificWebJournals" web portal hosted by "DergiPark" belongs to the author(s).
Correction, Withdrawal, Expression of Concern
If minor errors are detected that do not affect the results, comments and conclusions of the published article, the editor accepts the correction. If major errors and/or abuses are detected by the editor, which invalidates the results and conclusions, their withdrawal is considered.
If there is evidence of research or publication abuse by the authors, there is evidence that the findings are unreliable and that the authors' institutions did not investigate the incident, or the potential investigation seems unfair or inconclusive. The editor should consider issuing a statement of concern.
COPE and ICJME guidelines are considered regarding correction, withdrawal or expression of concern.
Archiving Policy
To preserve and permanently access all articles published in the journal "Aquatic Research", the papers are nationally archived under the name Dergipark (https://dergipark.org) by TÜBİTAK Turkish Academic Network and Information Center (ULAKBİM) affiliated with the Ministry of Industry and Technology of Türkiye. It is stored with a system established for journal publishing activities that allow LOCKSS to collect, preserve and present the content.
Additionally, authors are encouraged to self-archive the final PDF version of their articles in open electronic archives with that conform to standards of Open Archives Initiative (https://www.openarchives.org/). Authors should provide a link from the deposited version to the URL of journal website.

Processing and publication are free of charge with the journal. There is no article processing charges or submission fees for any submitted or accepted articles.

Open Access Statement:

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

Citation Indexes

TR Dizin
Scopus

Other Indexes

Zoological Record

Sorumlu Editör

Özkan Özden
Özkan ÖZDEN
Food Properties, Food Engineering, Food Sciences, Aquaculture and Fisheries, Post-Harvest Fisheries Technologies (Incl. Transportation), Fisheries Technologies

Sorumlu Editör Yardımcısı

Nuray Erkan
Nuray ERKAN
Nutrition and Dietetics, Public Health, Food Sciences, Food Packaging, Preservation and Processing, Food Biotechnology, Food Safety, Traceability, Certification and Authenticity, Food Chemistry and Food Sensory Science, Food Microbiology, Food Technology, Post-Harvest Fisheries Technologies (Incl. Transportation), Veterinary Food Hygiene and Technology

Language Editors

Gökhan Tunçelli
Gökhan TUNÇELLİ
Fish Breeding, Pisciculture, Shellfish Culture, Fisheries Technologies, Aquaculture

Ethics Editor

Şafak Ulusoy
Şafak ULUSOY
Aquatic Toxicology , Food Consumption, Fermentation Technology, Food Packaging, Preservation and Processing, Food Safety, Traceability, Certification and Authenticity, Food Microbiology, Food Technology, Drying Technologies, Fisheries Technologies

Editörler Kurulu

Albert Ukaro Ofuoku
Albert Ukaro OFUOKU
Agricultural Extension and Communication
Erhan Mutlu
Erhan MUTLU
Ecology, Oceanography
Murat Yiğit
Murat YİĞİT
Ecological Applications, Water Quality and Water Pollution, Surface Water Quality Processes and Contaminated Sediment Assessment, Aquaculture and Fisheries, Pisciculture, Farm Enterprises, Agribusiness
Default avatar
Jualang Azlan GANSAU
Plant Biotechnology in Agriculture
Emine Gözde Özbayram
Emine Gözde ÖZBAYRAM
Microbiology, Environmental Biotechnology, Environmental Biotechnology Diagnostics (Incl. Biosensors), Water Quality and Water Pollution, Environmental Engineering, Renewable Energy Resources
Default avatar
Eltjon HALİMİ
Entomology, Invertebrate Biology
Ulfert Focken
Ulfert FOCKEN
Pisciculture, Aquaculture and Fisheries (Other)
Momin Momin
Momin MOMİN
Fish Biology, Fish Physiology and Genetics, Fish Breeding, Pisciculture, Aquaculture
Default avatar
Katya PEYCHEVA
Analytical Biochemistry, Environmental Assessment and Monitoring, Food Safety, Traceability, Certification and Authenticity, Aquaculture
Adrian Grozea
Adrian GROZEA
Hydrobiology, Water Quality and Water Pollution, Aquaculture and Fisheries, Pisciculture
Emre Çağlak
Emre ÇAĞLAK
Food Packaging, Preservation and Processing, Food Biotechnology, Food Safety, Traceability, Certification and Authenticity, Food Chemistry and Food Sensory Science, Food Microbiology, Post-Harvest Fisheries Technologies (Incl. Transportation), Fisheries Technologies

16291

is licensed under a CreativeCommons Attribtion-ShareAlike 4.0 International Licence 14628   1325927040

Diamond Open Access refers to a scholarly publication model in which journals and platforms do not charge fees to either authors or readers.

Open Access Statement:

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

Archiving Policy:

Archiving is done according to TÜBİTAK ULAKBİM "DergiPark" publication policy (LOCKSS).