Recent Cytogenetic findings for the east Atlantic peacock wrasse, Symphodus tinca (Labridae, Teleostei), rarely observed in the Black Sea: Analysis of constitutive heterochromatin and rDNA gene regions

Serkan Saygun

doi:10.3153/AR26005

EN

Recent Cytogenetic findings for the east Atlantic peacock wrasse, Symphodus tinca (Labridae, Teleostei), rarely observed in the Black Sea: Analysis of constitutive heterochromatin and rDNA gene regions

Abstract

This study employed a 3-hour in vitro method using PB-MAX culture medium on kidney tissues from a rare East Atlantic peacock wrasse, Symphodus tinca (Linnaeus, 1758), collected from the Black Sea (Fatsa Bay, Ordu). The specimen was obtained as a single fish caught in a fisherman's net. Slide preparations were made by standard air-drying. High-quality metaphase slides were subjected to conventional C-banding, AgNO3 staining, and GTG-banding, with various modifications. Metaphases were karyotyped using AKAS Multispecies software, and idiograms were generated. Analysis of metaphase chromosomes revealed a common 2n=48 (NF=74) karyotype, with 6 metacentric, 20 submetacentric, 14 subtelocentric, and 8 acrocentric chromosomes. Sequential conventional staining first identified C+, NOR+, and G+ chromosome regions. These findings will support future molecular cytogenetic research, particularly regarding chromosomal evolution, genome differences within and between species, and taxonomic classification.

Keywords

Supporting Institution

This work was supported by the [Ordu University Scientific Research Projects Coordination Office] under Grant [AR-2350] and [TUBITAK] under Grant [123Z621].

Project Number

ODÜ BAP AR-2350, TUBITAK 1002 B 123Z621

Ethical Statement

According to the decision (Number: 82678388 and Date: 26.10.2021) taken by Ordu University Animal Experiments Ethics Committee, this research is not subject to AEEC permission and therefore does not require an ethical approval document.

Thanks

We thank Prof. Dr. Roberto Ferreira ARTONI and Asst. Prof. Dr. Cristian ARAYA-JAIME for their feedback on the study's method and evaluation.

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Details

Primary Language

English

Subjects

Hydrobiology , Fish Physiology and Genetics

Journal Section

Research Article

Authors

Serkan Saygun ^*
0000-0002-9789-3284
Türkiye

Early Pub Date

January 15, 2026

Publication Date

January 15, 2026

Submission Date

November 8, 2025

Acceptance Date

January 8, 2026

Published in Issue

Year 2026 Volume: 9 Number: 1

DOI

https://doi.org/10.3153/AR26005

IZ

https://izlik.org/JA65BB43LR

Cite

RIS / Bibtex

APA

Saygun, S. (2026). Recent Cytogenetic findings for the east Atlantic peacock wrasse, Symphodus tinca (Labridae, Teleostei), rarely observed in the Black Sea: Analysis of constitutive heterochromatin and rDNA gene regions. Aquatic Research, 9(1), 39-53. https://doi.org/10.3153/AR26005

Öz

Recent Cytogenetic findings for the east Atlantic peacock wrasse, Symphodus tinca (Labridae, Teleostei), rarely observed in the Black Sea: Analysis of constitutive heterochromatin and rDNA gene regions

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite