Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic

Aslı Çilingir Yeltekin

doi:10.3153/AR25026

EN

Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic

Abstract

Arsenic, a metalloid and metal compound commonly found in the earth's crust, dissolves in underground waters and can cause toxic effects by spreading through industrial processes or the environment. In this study, the changes in levels of antioxidant enzymes and several parameters (GSH-Px, CAT, MDA, SOD, and 8-OHdG) in the muscle tissue of juvenile trout (Oncorhynchus mykiss) after arsenic exposure were investigated, and the data obtained were analysed. In our study, juvenile trout were divided into three groups, and arsenic was administered at saturations of 25, 50, and 75 mg/L. 96 hours after application, the muscle tissue from young trout was removed and homogenised. The 8-OHdG content in the muscle tissue was determined using an ELISA kit. The levels of SOD, CAT, GSH-Px and MDA were determined using spectrophotometric methods. In young trout with arsenic accumulation in muscle tissue, DNA damage was observed, and the 8-OHdG content increased accordingly. There was deterioration in the cell membrane structure and lipid peroxidation. When the muscle tissue of young trout treated with arsenic was examined, a significant decrease in the concentrations of CAT, GSH-Px and SOD was observed. It is assumed that this is due to oxidative stress caused by the accumulation of free radicals in the muscle tissue of the fish. These changes occurred after arsenic was applied. The presence of heavy metals in large quantities in the habitats of aquatic organisms negatively affects their life and developmental stages, and their presence in the food chain and use as food by other species can cause significant toxicity.

Keywords

Supporting Institution

This study was supported by YYU Scientific Research Projects Presidency as project number FYL-2021-9693.

Project Number

FYL-2021-9693

Ethical Statement

The study was approved in accordance with the decision of the Van Yüzüncü Yıl University Animal Experiments Local Ethics Committee dated 29/04/2021, numbered 2021/04-19.

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Details

Primary Language

English

Subjects

Fish Biology

Journal Section

Research Article

Authors

Aslı Çilingir Yeltekin ^*
0000-0003-0071-7434
Türkiye

Early Pub Date

October 19, 2025

Publication Date

October 21, 2025

Submission Date

July 31, 2025

Acceptance Date

October 2, 2025

Published in Issue

Year 2025 Volume: 8 Number: 4

DOI

https://doi.org/10.3153/AR25026

IZ

https://izlik.org/JA38CZ52KR

Cite

RIS / Bibtex

APA

Çilingir Yeltekin, A. (2025). Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic. Aquatic Research, 8(4), 265-271. https://doi.org/10.3153/AR25026

Öz

Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite