Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic

Aslı Çilingir Yeltekin

doi:10.3153/AR25026

EN

Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic

Abstract

Arsenic, a metalloid and metal compound commonly found in the earth's crust, dissolves in underground waters and can cause toxic effects by spreading through industrial processes or the environment. In this study, the changes in levels of antioxidant enzymes and several parameters (GSH-Px, CAT, MDA, SOD, and 8-OHdG) in the muscle tissue of juvenile trout (Oncorhynchus mykiss) after arsenic exposure were investigated, and the data obtained were analysed. In our study, juvenile trout were divided into three groups, and arsenic was administered at saturations of 25, 50, and 75 mg/L. 96 hours after application, the muscle tissue from young trout was removed and homogenised. The 8-OHdG content in the muscle tissue was determined using an ELISA kit. The levels of SOD, CAT, GSH-Px and MDA were determined using spectrophotometric methods. In young trout with arsenic accumulation in muscle tissue, DNA damage was observed, and the 8-OHdG content increased accordingly. There was deterioration in the cell membrane structure and lipid peroxidation. When the muscle tissue of young trout treated with arsenic was examined, a significant decrease in the concentrations of CAT, GSH-Px and SOD was observed. It is assumed that this is due to oxidative stress caused by the accumulation of free radicals in the muscle tissue of the fish. These changes occurred after arsenic was applied. The presence of heavy metals in large quantities in the habitats of aquatic organisms negatively affects their life and developmental stages, and their presence in the food chain and use as food by other species can cause significant toxicity.

Keywords

Supporting Institution

This study was supported by YYU Scientific Research Projects Presidency as project number FYL-2021-9693.

Project Number

FYL-2021-9693

Ethical Statement

The study was approved in accordance with the decision of the Van Yüzüncü Yıl University Animal Experiments Local Ethics Committee dated 29/04/2021, numbered 2021/04-19.

References

Abdel-Gawad, F.K., Khalil, W.K., Bassem, S.M., Kumar, V., Parisi, C., Inglese, S., Guerriero, G. (2020). The duckweed, lemna minor modulates heavy metal-induced oxidative stress in the Nile Tilapia, Oreochromis niloticus. Water, 12(11), 2983. https://doi.org/10.3390/w12112983
Aggarwal, B.B. (2004). Nuclear factor-κB: The enemy within. Cancer Cell, 6(3), 203-208. https://doi.org/10.1016/j.ccr.2004.09.003
Atmaca, E., Aksoy, A. (2009). Oksidatif DNA hasarı ve kromatografik yöntemlerle tespit edilmesi. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 20(2), 79-83.
Baskan, M.B., Pala, A. (2009). Determination of arsenic removal efficiency by ferric ions using response surface methodology. Journal of Hazardous Materials, 166(2-3), 796-801. https://doi.org/10.1016/j.jhazmat.2008.11.131
Berg, M., Con, T.H., Nguyen, T.C., Pham, H.V., Schertenleib, R., Giger, W. (2001). Arsenic contamination of groundwater and drinking water in Vietnam: A human health Threat. Environmental Science & Technology, 35(13). https://doi.org/10.1021/es010027y
Cilingir Yeltekin, A. (2018). Comparison of toxic metal, trace element and macro element levels in trout cultivated in Latvia and Turkey. Fresenius Environmental Bulletin, 27(10), 7039-7044.
Doğan, D., Meydan, I., Kömüroğlu, A. U. (2022). Protective effect of silymarin and gallic acid against cisplatin-induced nephrotoxicity and hepatotoxicity. International Journal of Clinical Practice, 2022, 10. https://doi.org/10.1155/2022/6541026
El-Tekreti, S.A.A., Yeltekin, A.Ç. (2023). Determination of lithium toxicity by apoptosis and different biomarkers on Carassius auratus tissues. Indian Journal of Experimental Biology (IJEB), 61(09), 712-716. https://doi.org/10.56042/ijeb.v61i09.5115

Fernández, M.I., López, J.F., Vivaldi, B., Coz, F. (2012). Long-term impact of arsenic in drinking water on bladder cancer health care and mortality rates 20 years after end of exposure. The Journal of Urology, 187(3), 856-861. https://doi.org/10.1016/j.juro.2011.10.157
Hematyar, N., Rustad, T., Sampels, S., Dalsgaard, K.T. (2019). Relationship between lipid and protein oxidation in fish. Aquaculture Research, 50(5), 1393-1403. https://doi.org/10.1111/are.14012
Hughes, M.F. (2002). Arsenic toxicity and potential mechanisms of action. Toxicology Letters, 133, 1–16. https://doi.org/10.1016/S0378-4274(02)00084-X
Iyorah, I., Enagbonma, B.J., Abu, O., Biose, E. (2023). Oxidative stress responses to heavy metal burden in African catfish (Clarias gariepinus) from Warri River, Niger-Delta, Southern Nigeria. Sokoto Journal of Veterinary Sciences, 21(2), 74-82. https://doi.org/10.4314/sokjvs.v21i2.4
Bhat, R.A., Bakhshalizadeh, S., Guerrera, M.C., Kesbic, O.S., Fazio, F. (2023). Toxic effect of heavy metals on ovarian deformities, apoptotic changes, oxidative stress, and steroid hormones in rainbow trout. Journal of Trace Elements in Medicine and Biology, 75, 127106. https://doi.org/10.1016/j.jtemb.2022.127106
Lau, C., Lu, X., Chen, X., Hoy, K.S., Davydiuk, T., Jennifer, A.G., Reichert, M., LeBlanc, A., Donadt, C., Jhangri, G.X., Le, C. (2025). Arsenic speciation in more than 1600 freshwater fish samples from fifty-three waterbodies in Alberta, Canada, Journal of Environmental Sciences, 153, 289-301. https://doi.org/10.1016/j.jes.2024.12.009
Lee, J.W., Jo, A.H., Lee, D.C., Choi, C.Y., Kang, J.C., Kim, J.H. (2023). Review of cadmium toxicity effects on fish: Oxidative stress and immune responses. Environmental Research, 236, 116600. https://doi.org/10.1016/j.envres.2023.116600
Li, M., Kong, Y., Wu, X., Yin, Z., Niu, X., Wang, G. (2021). Dietary α-lipoic acid can alleviate the bioaccumulation, oxidative stress, cell apoptosis, and inflammation induced by lead (Pb) in Channa argus. Fish & Shellfish Immunology, 119, 249-261. https://doi.org/10.1016/j.fsi.2021.10.010
Lu, H., Zhang, Y., Zhang, X., Wang, Y., Guo, T., Wang, Q., Zhao, H., Xing, M. (2024). New insights into zinc alleviating renal toxicity of arsenic-exposed carp (Cyprinus carpio) through YAP-TFR/ROS signalling pathway. Pesticide Biochemistry and Physiology, 205, 106153. https://doi.org/10.1016/j.pestbp.2024.106153
Méndez-Armenta, M., Nava-Ruiz, C., Fernández-Valverde, F., Sánchez-García, A., Rios, C. (2011). Histochemical changes in muscle of rats exposed subchronically to low doses of heavy metals. Environmental Toxicology and Pharmacology, 32(1), 107-112. https://doi.org/10.1016/j.etap.2011.03.001
Özdek, U., Yıldırım, S., Değer, Y. (2020). The effect of Diplotaenia turcica root extract in streptozotocininduced diabetic rats. Turkish Journal of Biochemistry, 45(2), 1-10. https://doi.org/10.1515/tjb-2018-0411
Özcan, O., Erdal, H., Çakırca, G., Yönden, Z. (2015). Oxidative stress and its impacts on intracellular lipids, proteins and DNA. Journal of Clinical Trials and Experimental Investigations, 6(3), 331-336. https://doi.org/10.5799/ahinjs.01.2015.03.0545
Rachamalla, M., Salahinejad, A., Kodzhahinchev, V., Niyogi, S. (2024). Reproductive and developmental effects of sex-specific chronic exposure to dietary arsenic in zebrafish (Danio rerio). Toxics, 12(4), 302. https://doi.org/10.3390/toxics12040302
Sancar, A., Lindsey-Boltz, L.A., Ünsal-Kaçmaz, K., Linn, S. (2004). Molecular mechanisms of mammalian DNA repair and the damage checkpoints. Annual Review of Biochemistry, 73, 39-85. https://doi.org/10.1146/annurev.biochem.73.011303.073723
Saç, H., Yeltekin A.Ç. (2023). Investigation of oxidative stress status and apoptotic markers of juvenile trout exposed to arsenic toxicity. Toxicology Research, 12(4), 608-614. https://doi.org/10.1093/toxres/tfad049
Wagh, M.S., Osborne, W.J., Sivarajan, S. (2023). Toxicity assessment of lead, nickel and cadmium on zebra fish augmented with Bacillus xiamenensis VITMSJ3: An insight on the defense mechanism against oxidative stress due to heavy metals. Food and Chemical Toxicology, 177, 113830. https://doi.org/10.1016/j.fct.2023.113830 Wang, J., Xiao, J., Zhang, J., Chen, H., Li, D., Li, L., Luo, Y. (2020). Effects of dietary Cu and Zn on the accumulation, oxidative stress and the expressions of immune-related genes in the livers of Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 100, 198-207. https://doi.org/10.1016/j.fsi.2020.03.012
Yao, Q., Yang, A., Hu, X., Zou, H., Chen, J., Li, Q., Li, C. (2023). Effects of antimony exposure on DNA damage and genome-wide variation in zebrafish (Danio rerio) liver. Aquatic Toxicology, 259, 106524. https://doi.org/10.1016/j.aquatox.2023.106524
Yeltekin, A.Ç. (2024). Assessment of Apoptosis and DNA damage in Alburnus tarichi exposed to the toxicity of the fungicide azole. Biology Bulletin, 51(6), 1549-1555. https://doi.org/10.1134/S1062359024608176
Yeltekin, A.Ç. (2022). The responses of cholinergic system in the brain tissue of Van Fish (Alburnus tarichi) exposed to antifungal tebuconazole compound toxicity. Aquatic Research, 5(2), 110-116. https://doi.org/10.3153/AR22010

Details

Primary Language

English

Subjects

Fish Biology

Journal Section

Research Article

Authors

Aslı Çilingir Yeltekin ^*
0000-0003-0071-7434
Türkiye

Early Pub Date

October 19, 2025

Publication Date

October 21, 2025

Submission Date

July 31, 2025

Acceptance Date

October 2, 2025

Published in Issue

Year 1970 Volume: 8 Number: 4

DOI

https://doi.org/10.3153/AR25026

IZ

https://izlik.org/JA38CZ52KR

Cite

RIS / Bibtex

APA

Çilingir Yeltekin, A. (2025). Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic. Aquatic Research, 8(4), 265-271. https://doi.org/10.3153/AR25026

Öz

Investigation of oxidative stress, lipid peroxidation and DNA damage in the muscle tissue of young trout treated with arsenic

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite