Evaluation of the anti-Acanthamoeba potential of Myriophyllum spicatum on Acanthamoeba castellanii trophozoites

Beyhan Taş; Zeynep Kolören; Onur Kolören

doi:10.3153/AR25017

EN

Evaluation of the anti-Acanthamoeba potential of Myriophyllum spicatum on Acanthamoeba castellanii trophozoites

Abstract

Myriophyllum spicatum is a submerged aquatic macrophyte known for its allelopathic and antimicrobial properties. This study investigated the amoebicidal activity of ethanolic M. spicatum leaf extract against Acanthamoeba castellanii trophozoites. Plant samples were collected from the Terme River (Samsun), extracted with ethanol, and tested at concentrations ranging from 1.5 to 48 µg/mL over 24, 48, and 72 hours. Trophozoite viability was assessed using trypan blue staining, and statistical analysis was performed with SPSS and Jamovi software. Results showed significant dose- and time-dependent reductions in trophozoite viability, with an IC50 value of 26.5 µg/mL at 72 hours. The highest inhibition was observed at 48 µg/mL, resulting in a 33% reduction in viability. Principal Component Analysis revealed distinct clustering of higher concentrations from lower doses and control. The study highlights M. spicatum’s potential as a natural anti-amoebic agent. Given the parasite's resistance to conventional drugs and the increasing threat of Acanthamoeba infections, particularly in developing regions, plant-based compounds offer promising alternatives. Further research is recommended to evaluate the extract’s effects on the cyst stage and explore its therapeutic applications. This study is the first to report the anti-Acanthamoeba effect of M. spicatum, contributing novel insight into aquatic plant-based antiparasitic agents.

Keywords

Ethical Statement

Ethics committee approval is not required for this study.

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Details

Primary Language

English

Subjects

Food Microbiology

Journal Section

Research Article

Authors

Beyhan Taş
0000-0001-6421-2561
Türkiye

Zeynep Kolören ^*
0000-0001-9708-2716
Türkiye

Onur Kolören
0000-0001-7845-6647
Türkiye

Early Pub Date

June 27, 2025

Publication Date

July 2, 2025

Submission Date

March 25, 2025

Acceptance Date

April 12, 2025

Published in Issue

Year 1970 Volume: 8 Number: 3

DOI

https://doi.org/10.3153/AR25017

IZ

https://izlik.org/JA24RZ96WF

Cite

RIS / Bibtex

APA

Taş, B., Kolören, Z., & Kolören, O. (2025). Evaluation of the anti-Acanthamoeba potential of Myriophyllum spicatum on Acanthamoeba castellanii trophozoites. Aquatic Research, 8(3), 166-175. https://doi.org/10.3153/AR25017

Öz

Evaluation of the anti-Acanthamoeba potential of Myriophyllum spicatum on Acanthamoeba castellanii trophozoites

Abstract

Keywords

Ethical Statement

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite