Studies on organogenesis of Cyprinus carpio var. koi with reference to histological perspectives

Sivakumar Kandhasamy; Muthupriya Palanichamy; Meenakshi Sivalingam Valliappan; Kareem Altaff

doi:10.3153/AR25004

TR EN

Studies on organogenesis of common carp Cyprinus carpio var. koi with reference to histological perspectives

Öz

The objective of the current study was to investigate the histological characteristics of larval organogenesis in Cyprinus carpio var. koi. The eggs and several developmental stages of C. carpio var. koi larvae were preserved in Bouin's fluid. Histological examinations were conducted daily from the first day after fertilisation (DAF) to the 12th DAF and the 16th, 24th, 32nd, and 40th DAF. Histological examinations of C. carpio var. koi eggs revealed the development of an embryonic disc, followed by the formation of the neural tube and embryonic eyes on the second day after fertilisation (DAF). The just-emerged larvae have distinct eyes and a neural tube that has undergone differentiation into the brain. Following that, an examination was conducted on the development of the digestive system, swim bladder, gills, skin, and fins. The ontogeny developments were categorised into five distinct stages. During larval development, the initial two stages have exhibited variations in their organogenesis. The larvae head was aligned and devoid of the yolk, but their body was connected to the yolk sac and gill arches, which were visible between the 3rd and 5th days after fertilisation (DAF), and their total length range was 4.35-6.83mm. The digestive system had a linear configuration, with an enlargement in both the dorsal and ventral regions of the body on the ninth day after fertilisation (DAF). During the 10th to 17th developmental stages, larvae have a total length range of 11.23-14.35mm. The operculum, gill lamella, and dorsal fin were developed in this stage. Between the 24th and 40th days after fertilisation (DAF), the larvae acquired a fin, a coiled gut, and well-developed accessory glands and their total length was measured between 15.01 and 23.68mm.

Anahtar Kelimeler

Studies on organogenesis of Cyprinus carpio var. koi with reference to histological perspectives

Abstract

The objective of the current study was to investigate the histological characteristics of larval organogenesis in Cyprinus carpio var. koi. The eggs and several developmental stages of C. carpio var. koi larvae were preserved in Bouin's fluid. Histological examinations were conducted daily from the first day after fertilisation (DAF) to the 12th DAF and the 16th, 24th, 32nd, and 40th DAF. Histological examinations of C. carpio var. koi eggs revealed the development of an embryonic disc, followed by the formation of the neural tube and embryonic eyes on the second day after fertilisation (DAF). The just-emerged larvae have distinct eyes and a neural tube that has undergone differentiation into the brain. Following that, an examination was conducted on the development of the digestive system, swim bladder, gills, skin, and fins. The ontogeny developments were categorised into five distinct stages. During larval development, the initial two stages have exhibited variations in their organogenesis. The larvae head was aligned and devoid of the yolk, but their body was connected to the yolk sac and gill arches, which were visible between the 3rd and 5th days after fertilisation (DAF), and their total length range was 4.35-6.83mm. The digestive system had a linear configuration, with an enlargement in both the dorsal and ventral regions of the body on the ninth day after fertilisation (DAF). During the 10th to 17th developmental stages, larvae have a total length range of 11.23-14.35mm. The operculum, gill lamella, and dorsal fin were developed in this stage. Between the 24th and 40th days after fertilisation (DAF), the larvae acquired a fin, a coiled gut, and well-developed accessory glands and their total length was measured between 15.01 and 23.68mm.

Keywords

Supporting Institution

Karpaga Vinayaga College of Engineering and Technology & The New College

Ethical Statement

The experiments were conducted in accordance with the guidelines and regulations established by the Committee for Control and Supervision of Experiments on Animals (CCSEA), Department of Animal Husbandry and Dairying, Ministry of Fisheries, Animal Husbandry and Dairying, Government of India and the experimental protocol was approved by Institutional Animal Ethical Committee (Karpaga Vinayaga Institute of Medical Sciences and Research Institute, Tamil Nadu, India) (No: 181GO/ERE/S/15/CPCSEA dated 04.12.2018).

Thanks

We would like to the Managements, the Principals of Karpaga Vinayaga College of Engineering and Technology, Chengalpattu – 603308 and The New College, Chennai - 600014 for providing laboratory facilities to complete the work.

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Details

Primary Language

English

Subjects

Fish Anatomy

Journal Section

Research Article

Authors

Sivakumar Kandhasamy ^*
0000-0001-8971-869X
India

Muthupriya Palanichamy
0000-0002-7859-9290
India

Meenakshi Sivalingam Valliappan
0009-0007-7714-1732
India

Kareem Altaff
0000-0001-9178-2519
India

Early Pub Date

December 11, 2024

Publication Date

January 7, 2025

Submission Date

June 17, 2024

Acceptance Date

August 14, 2024

Published in Issue

Year 1970 Volume: 8 Number: 1

DOI

https://doi.org/10.3153/AR25004

IZ

https://izlik.org/JA36GN58JA

Cite

RIS / Bibtex

APA

Kandhasamy, S., Palanichamy, M., Sivalingam Valliappan, M., & Altaff, K. (2025). Studies on organogenesis of Cyprinus carpio var. koi with reference to histological perspectives. Aquatic Research, 8(1), 39-51. https://doi.org/10.3153/AR25004