In vitro, amoebicidal activities of submerged plant Ceratophyllum demersum L. extract against Acanthamoeba castellanii trophozoites

Beyhan Taş; Zeynep Kolören; Onur Kolören

doi:10.3153/AR24016

EN

In vitro, amoebicidal activities of submerged plant Ceratophyllum demersum L. extract against Acanthamoeba castellanii trophozoites

Abstract

Ceratophyllum demersum L. is a hydrophyte with potential for use as an analgesic, antipyretic and anti-inflammatory drug. It has also been stated that it is a hepatoprotective and anti-diarrheal agent with potential use in stomach disorders and respiratory diseases. Our study investigated the anti-amoebic activity of C. demersum, which became a hydrophyte underwater collected from Samsun River Miliç. Different concentrations of the pathogenic strain of Acanthamoeba castellanii (ATCC 30010) and the ethanolic extract of C. demersum were used to determine anti-Acanthamoeba activity. A. castellanii trophozoites were treated with C. demersum extract at different concentrations ranging from 1.9, 3.8, 7.6, 15.2, 30.4, 60.8 µg/mL and incubated at 26°C for 72 h. The 50% inhibitory concentration (IC50) of C. demersum extract was approximately 42.5 µg/mL at 72 hours. Trophozoite cell viability decreased to 42% and 58.33% in the presence of 30.4 and 60.8 µg/mL C. demersum extract at 72 hours, respectively. These results indicate that the ethanolic extract of C. demersum has anti-Acanthamoeba activity against A. castellanii trophozoites. The study highlights that this extract can be a potential protective drug source against Acanthamoeba trophozoites.

Keywords

Ethical Statement

This study does not require ethics committee permission or any special permission.

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Details

Primary Language

English

Subjects

Food Microbiology

Journal Section

Research Article

Authors

Beyhan Taş
0000-0001-6421-2561
Türkiye

Zeynep Kolören ^*
0000-0001-9708-2716
Türkiye

Onur Kolören
0000-0001-7845-6647
Türkiye

Early Pub Date

August 15, 2024

Publication Date

October 6, 2024

Submission Date

February 21, 2024

Acceptance Date

May 10, 2024

Published in Issue

Year 2024 Volume: 7 Number: 4

DOI

https://doi.org/10.3153/AR24016

IZ

https://izlik.org/JA28XU65EK

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RIS / Bibtex

APA

Taş, B., Kolören, Z., & Kolören, O. (2024). In vitro, amoebicidal activities of submerged plant Ceratophyllum demersum L. extract against Acanthamoeba castellanii trophozoites. Aquatic Research, 7(4), 178-188. https://doi.org/10.3153/AR24016

Öz

In vitro, amoebicidal activities of submerged plant Ceratophyllum demersum L. extract against Acanthamoeba castellanii trophozoites

Abstract

Keywords

Ethical Statement

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite