Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

Arif Parmaksız

doi:10.3153/AR23003

EN

Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

Abstract

Arabibarbus grypus (Heckel, 1843), which naturally lives in the Euphrates and Tigris River systems and is endemic, is an economically important fish species consumed by humans. Since the population of this species, which is hunted by both fishermen and local people, is decreasing day by day, its genetic characteristics need to be determined. This study aims to determine the genetic characteristics of A. grypus samples living in Great Zab based on sequence analysis of mtDNA cyt b and mtDNA d loop gene regions. Total DNA isolation was performed from muscle tissue using the kit. Then, Polymerase Chain Reaction was applied through specific primers to mtDNA cyt b and mtDNA d loop gene regions, and the target regions were amplified. The products with the target length were sent to the commercial firm and sequence analysis was performed. Regarding these specimens living in Great Zab, two haplotypes were determined for the mtDNA cyt b gene region and five haplotypes for the mtDNA d loop gene region. These haplotypes were compared with the haplotypes in the gene bank and the results were evaluated. Some important data has been obtained regarding the conservation and management of this fish species. Since three new haplotypes were detected for the d loop region in this studied locality, it is important to include the samples in this locality in conservation studies.

Keywords

References

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Details

Primary Language

English

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Arif Parmaksız ^*
0000-0003-0321-8198
Türkiye

Publication Date

January 1, 2023

Submission Date

September 1, 2022

Acceptance Date

October 18, 2022

Published in Issue

Year 2023 Volume: 6 Number: 1

DOI

https://doi.org/10.3153/AR23003

IZ

https://izlik.org/JA32HW53AS

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RIS / Bibtex

APA

Parmaksız, A. (2023). Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq). Aquatic Research, 6(1), 19-25. https://doi.org/10.3153/AR23003

Öz

Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

Abstract

Keywords

References

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Primary Language

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Authors

Publication Date

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Acceptance Date

Published in Issue

DOI

IZ

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