Monitoring of growth and biochemical composition of Dunaliella salina and Dunaliella polymorpha in different photobioreactors

Zeliha Demirel

doi:10.3153/AR22013

EN

Monitoring of growth and biochemical composition of Dunaliella salina and Dunaliella polymorpha in different photobioreactors

Abstract

In this study, the isolation of green algae were collected from two different stations of Aegean Sea and Seyfe Lake. The molecular identification of Dunaliella species using their 18S ribosomal DNA genes were sequenced and investigated with the BLAST program in the NCBI database. After the morphological and molecular identification, two different Dunaliella species were deposited in Ege University Microalgae Culture Collection. D. salina and D. polymorpha cells were firstly produce in both bubble column to monitor the growth profiles and then the species were cultivated in bubble column and stirred column photobioreactors (PBRs) under both high light intensity and different mixing conditions to investigate the total protein, carbohydrate, lipids and carotenoid concentrations. Moreover, this study aims to evaluate the production of β-carotene using two different PBRs. As a result of this study, D. salina in stirred PBR obtained the highest lipid (334.79 ±0.02 mg/L), total carotenoid (96.7 ±0.02 mg/L), and β-carotene content (21.18 ±0.03 µg/mL), while the maximum dry cell mass of 0.906 g/L was reached by D. polymorpha in bubble column PBR. The aim of this study was to investigate the nutritional values and β-carotene content of Dunaliella salina and D. polymorpha isolated from Turkey.

Keywords

Thanks

The Author thanks Dr. Zinar Pinar Gumus for performing the HPLC-DAD analysis.

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Details

Primary Language

English

Subjects

Hydrobiology , Maritime Engineering (Other)

Journal Section

Research Article

Authors

Zeliha Demirel ^*
0000-0003-3675-7315
Türkiye

Publication Date

April 1, 2022

Submission Date

December 9, 2021

Acceptance Date

March 3, 2022

Published in Issue

Year 2022 Volume: 5 Number: 2

DOI

https://doi.org/10.3153/AR22013

IZ

https://izlik.org/JA47TA73JD

Cite

RIS / Bibtex

APA

Demirel, Z. (2022). Monitoring of growth and biochemical composition of Dunaliella salina and Dunaliella polymorpha in different photobioreactors. Aquatic Research, 5(2), 136-145. https://doi.org/10.3153/AR22013

Öz

Monitoring of growth and biochemical composition of Dunaliella salina and Dunaliella polymorpha in different photobioreactors

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite