Research Article
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Bacteriological quality of cage-cultured abalone Haliotis asinina

Year 2021, , 151 - 159, 01.04.2021

Jhonamie Mabuhay-omar , Genese Divine B. Cayabo Lota A. Creencıa

https://doi.org/10.3153/AR21012

Abstract

Abalone is one of the most highly priced seafood delicacies and prepared in various dishes like breaded, soup, steamed and sashimi. They are susceptible to microbial contamination since it is eaten raw sometimes and pathogenic microorganisms can be hazardous to consumers. The present study was carried out to determine the coliform load and the presence of presumptive pathogenic bacteria in cage-cultured abalone in Taytay, Palawan, Philippines. The study was limited to the detection of coliform and some presumptive pathogenic bacteria in different parts of abalone such as gut, gills and mantle. The result of the study revealed that the count of coliforms present in the mantle and gills of abalone falls within the normal standard limit (7 – 21 MPN 100g-1 sample). On the other hand, the gut of abalone was beyond the standard limit (460 MPN 100g-1 sample). Moreover, the gut of abalone harbors Vibrio spp., Salmonella spp. and Shigella spp. and general enteric bacteria. Foodborne infections caused by Vibrio, Salmonella and Shigella are common in Asia. 

Keywords

Abalone Cage culture Coliform Microbial load Most probable number Salmonella Vibrio

Supporting Institution

This study was funded by the Commission on Higher Education (CHED DARE TO) of the Philippines as approved by the Board of Regents (BOR) under the Resolution No. 325, series of 2017.

Project Number

315

References

  • Bakr, W.M.K., Hazzah, W.A., Abaza, A.F. (2011). Detection of Salmonella and Vibrio species in some seafood in Alexandria. Journal of American Science, 7(9), 663-668.
  • Borromeo, R.U. (2007). City health says grilled squid caused summit food poisoning. The Freeman. Retrieved from http://www.philstar.com/cebu-news/383788/city-health-says-grilled-squid-caused-summit-food-poisoning on 19 Oct 2020 (accessed 10.11 2020).
  • Bower, S.M. (2017). Synopsis of Infectious Diseases and Parasites of Commercially Exploited Shellfish: Bacterial Diseases of Abalone. https://www.dfo-mpo.gc.ca/science/aah-saa/diseases-maladies/vibrioab-eng.html (accessed 10.11 2020).
  • Brazilian Regulations. (2019). Technical regulation on microbiological standards for foods. Resolution – RDC No. 331. Ministry of Health, National Health Surveillance Agency,
  • Brown, A.E. (2005). Benson’s Microbiological Applications Complete Version: Laboratory Manual in General Microbiology, 9th ed. Mac Graw Hill Companies, Inc. N.Y.
  • Cai, J., Han, Y., Wang, Z. (2006). Isolation of Vibrio parahaemolyticus from abalone (Haliotis diversicolor supertaxa L.) postlarvae associated with mass mortalities. Aquaculture, 257, 161-166. https://doi.org/10.1016/j.aquaculture.2006.03.007
  • Centers for Disease Control and Prevention (CDC). (2010). Preliminary foodnet data on the incidence of infection with pathogens transmitted commonly through food. Morbidity and Mortality Weekly Report, 59 (14), 418-422.
  • Chinnadurai, S., Campos, C.J.A., Geethalakshmi, V., Sharma, J., Kripa, V., Mohamed, K.S. (2020). Microbiological quality of shellfish harvesting areas in the Ashtamudi and Vemband estuaries (India): Environmental influences and compliance with international standards. Marine Pollution Bulletin, 156, 1-9. https://doi.org/10.1016/j.marpolbul.2020.111255
  • Cook, P.A. (2016). Recent trends in worldwide abalone production. Journal of Shellfish Research, 35(3), 581-583. https://doi.org/10.2983/035.035.0302
  • Duncan, P.F., Andalecio, M.N., Peralta, E., Laureta, L., Hidalgo, A.R., Napata, R. (2009). Evaluation of production technology, product quality and market potential for the development of bivalve mollusc aquaculture in the Philippines. Australian Centre for International Agricultural Research (ACIAR), 193 pp.
  • Edun, O.M., Akinrotimi, O.A., Makinde, O.O. (2016). Seasonal changes of microbial load in some sea foos from Baguma and Ekerekana Creeks, Niger Delta, Nigeria. Peertechz Journal of Environmental Science and Toxicology, 1(1), 001-007. https://doi.org/10.17352/aest.000001
  • Encena, II V.C., Bayona, N.C. (2010). Farming of the tropical abalone Haliotis asinina. Aquaculture Extension Manual, 49, 1-24.
  • European Communities (EC). (2007). Commission regulation no. 1441/2007 of December 2007 amending regulation (EC) no. 2073/2005 on microbiological criteria for foodstuffs. Official Journal of the European Union, 322, 12-29. http://data.europa.eu/eli/reg/2007/1441/oj
  • Florini, S., Shahsavari, E., Ngo, T., Aburto-Medina, A., Smith, D.J., Ball, A.S. (2020). Factors influencing the concentration of fecal coliforms in oysters in the River Blackwater Estuary, UK. Water, 12, 1-12. https://doi.org/10.3390/w12041086
  • Food Safety Inspection Service (FSIS) (2014). Foodborne Illness: What Consumers Need to Know. Washington, DC: United States Department of Agriculture. Retrieved from http://www.fsis.usda.gov/wps/portal/fsis/topics/food-safety-education/get-answers/food-safety-fact-sheets/foodborne-illness-and-disease/foodborne-illness-what-consumers-need-to-know/CT_Index on 16 Oct 2020.
  • Food and Agriculture Organization (FAO) (2016). The State of World Fisheries and Aquaculture. Contributing to food security and nutrition for all. Rome. 200 pp. ISBN 978-92-5-109185-2.
  • Food and Agriculture Organization (FAO), World Health Organization (WHO) (2020). Risk assessment tools for Vibrio parahaemolyticus and Vibrio vulnificus associated with seafood. Microbiological Risk Assessment Series No. 20. Rome.
  • Food and Agriculture Organization (FAO), World Health Organization (WHO) (2011). Risk assessment of Vibrio parahaemolyticus in seafood: Interpretative summary and Technical report. Microbiological Risk Assessment Series, No 16, Rome.
  • Food and Agriculture Organization (FAO), World Health Organization (WHO) (2005). Risk assessment of Vibrio vulnificus in raw oysters: Interpretative summary and Technical report. Microbiological Risk Assessment Series, No 8, Rome.
  • Food Drugs Administration (FDA). (2009). National Shellfish Sanitation Program guide for the control of molluscan shellfish 2007 revisions. Retrieved from http://www.cfsan.fda.gov/;ear/nss4-toc.html (accessed 10.11 2017)
  • Gnanambal, K., Patterson, J. (2005). Biochemical and microbiological quality of frozen fishes available in Tuticorin supermarkets. Fishery Technology, 42(1), 83-84.
  • Handlinger, J.J., Donachie, C.L., Gabor, L., Taylor, D. (2005). Bacterial infection in Tasmanian farmed abalone: Causes, pathology, farm factors and control options. Diseases of Asian Aquaculture, 5, 289-299.
  • International Commission of Microbiological Specification for Food (ICMSF). (1986). Microorganisms in foods. Vol. 1: Their significance and methods of enumeration. 2nd edition, Thatcher F. S., Clark D. S. (eds), University of Toronto Press, Toronto, ON, Canada, pp. 19-30, 181.
  • Iwamoto, M., Ayers, T., Mahon, B.E., Swerdlow, D.L. (2010). Epidemiology of seafood-associated infections in the United States. Clinical Microbiology Reviews, 23(2), 399-411. https://doi.org/10.1128/CMR.00059-09
  • Kua, B.C., Ramly, R., Devakie, M., Groman, D., Berthe, C.J.F. (2011). Investigating a mortality in hatchery cultured tropical abalone, Haliotis asinina Linnaeus, 1758 in Malaysia. Diseases of Asian Aquaculture, 7, 103-109.
  • Lameira Silva, O.L., Veríssimo S.M.M., da Rosa, A.M.B.P., Iguchi, B.Y., Nunes, E.D.S.C.D.L., Moraes, C.M.D., Cordeiro, C.A.M., Xavier, D.D.A., Pinto, A.S.O., Peixoto Joele, M.R.S., Brito, J.D.S., Juen, L., Rocha, R.M.D. (2020). Effects of environmental factors on microbiological quality of oyster farming in Amazon estuaries. Aquaculture Reports, 18, 1-10. https://doi.org/10.1016/j.aqrep.2020.100437
  • Lee, K.K., Liu, P.C., Chen, Y.C., Huang, C.Y. (2001). The implication of ambient temperature with the outbreak of vibriosis in cultured small abalone Haliotis diversicolor supertaxa Lischke. Journal of Thermal Biology, 26, 585-587. https://doi.org/10.1016/S0306-4565(01)00004-3
  • Lee, M. J., Lee, J. J., Chung, H. Y., Choi, S. H., Kim, B. S. (2016). Analysis of microbiota on abalone (Haliotis discus hannai) in South Korea for improved product management. International journal of food microbiology, 234, 45-52. https://doi.org/10.1016/j.ijfoodmicro.2016.06.032
  • Mabuhay-Omar, J.A., Cayabo, G.D.B., Nuñala, I.J.P., Habal, S.E., Creencia, L.A. (2019). Microbial and microparasite abundance in cage-cultured abalone Haliotis asinina. Journal of Shellfish Research, 38(2), 405-411. https://doi.org/10.2983/035.038.0223
  • Martínez, O., Rodríguez-Calleja, J.M., Santos, J.A., Otero, A., García-López, M.L. (2009). Foodborne and indicator bacteria in farmed molluscan shellfish before and after depuration. Journal of Food Protection, 72(7), 1443-1449. https://doi.org/10.4315/0362-028x-72.7.1443
  • Philippine National Standards - Bureau of Agriculture and Fisheries Product standards (PNS-BAFPS). (2011). Philippine National Standards - Bureau of Agriculture and Fisheries Product Standards for Live and Raw Molluscs. PNS/BAFPS 89:2011, ICS 67.120.30.
  • Pitchon, D., Cudennec, B., Huchette, S., Djediat, C., Renault, T., Paillard, C., Auzoux-Bordenave, S. (2013). Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures. Cytotechnology, 65, 759-772. https://doi.org/10.1007/s10616-013-9583-1
  • Romalde, J.L., Diéguez, A.l., Lasa, A., Balboa, S. (2014). New Vibrio species associated to molluscan microbiota: a review. Frontiers in Microbiology, 4, 1-11. https://doi.org/10.3389/fmicb.2013.00413
  • Sanders, E.R. (2012). Aseptic laboratory techniques: Plating methods. Journal of Visualized Experiments, 63, 1-18. https://doi.org/10.3791/3064
  • Sanjee, S.A., Karim, M.E. (2016). Microbiological quality assessment of frozen fish and fish processing materials from Bangladesh. International Journal of Food Science, 1-6. https://doi.org/10.1155/2016/8605689
  • Santiago, C.H.S., Mabuhay-Omar, J.A. (2019). Isolation and characterization of antimicrobial-producing bacteria from the donkey's ear abalone Haliotis asinina, Journal of Shellfish Research, 38(2), 413-416. https://doi.org/10.2983/035.038.0224
  • Sorio, J.C., Peralta, J.P. (2018). Microbiological quality of oyster (Crassostrea iredalei) in selected production areas in Dumangas, Iloilo, Philippines. AACL Bioflux, 11(2), 319-326.
  • Suleria, H.A.R., Masci, P.P., Gobe, G.C., Osborne, S.A. (2017). Therapeutic potential of abalone and status of bioactive molecules: A comprehensive review. Critical Reviews in Food Science and Nutrition, 57(8), 1742-1748. https://doi.org/10.1080/10408398.2015.1031726
  • Surtida, A.P. (2000). Abalone. SEAFDEC Asian Aquaculture, 22(4), 14-16. World Health Organization (WHO). (2001). Water quality: Guidelines, standards and health. IWA Publishing, London, UK. ISBN: 1900222 28 0
  • World Health Organization (WHO). (1996). Weekly Epidemiological Record. 73, 201-208.
  • Yagoub, S.O., Ahmed, T.M. (2003). Pathogenic microorganisms in freshwater samples collected from Khartoum central market. Sudan Journal of Veterinary Science and Animal Husbandry, 43, 32–37.

Year 2021, , 151 - 159, 01.04.2021

Jhonamie Mabuhay-omar , Genese Divine B. Cayabo Lota A. Creencıa

https://doi.org/10.3153/AR21012

Abstract

Project Number

315

References

  • Bakr, W.M.K., Hazzah, W.A., Abaza, A.F. (2011). Detection of Salmonella and Vibrio species in some seafood in Alexandria. Journal of American Science, 7(9), 663-668.
  • Borromeo, R.U. (2007). City health says grilled squid caused summit food poisoning. The Freeman. Retrieved from http://www.philstar.com/cebu-news/383788/city-health-says-grilled-squid-caused-summit-food-poisoning on 19 Oct 2020 (accessed 10.11 2020).
  • Bower, S.M. (2017). Synopsis of Infectious Diseases and Parasites of Commercially Exploited Shellfish: Bacterial Diseases of Abalone. https://www.dfo-mpo.gc.ca/science/aah-saa/diseases-maladies/vibrioab-eng.html (accessed 10.11 2020).
  • Brazilian Regulations. (2019). Technical regulation on microbiological standards for foods. Resolution – RDC No. 331. Ministry of Health, National Health Surveillance Agency,
  • Brown, A.E. (2005). Benson’s Microbiological Applications Complete Version: Laboratory Manual in General Microbiology, 9th ed. Mac Graw Hill Companies, Inc. N.Y.
  • Cai, J., Han, Y., Wang, Z. (2006). Isolation of Vibrio parahaemolyticus from abalone (Haliotis diversicolor supertaxa L.) postlarvae associated with mass mortalities. Aquaculture, 257, 161-166. https://doi.org/10.1016/j.aquaculture.2006.03.007
  • Centers for Disease Control and Prevention (CDC). (2010). Preliminary foodnet data on the incidence of infection with pathogens transmitted commonly through food. Morbidity and Mortality Weekly Report, 59 (14), 418-422.
  • Chinnadurai, S., Campos, C.J.A., Geethalakshmi, V., Sharma, J., Kripa, V., Mohamed, K.S. (2020). Microbiological quality of shellfish harvesting areas in the Ashtamudi and Vemband estuaries (India): Environmental influences and compliance with international standards. Marine Pollution Bulletin, 156, 1-9. https://doi.org/10.1016/j.marpolbul.2020.111255
  • Cook, P.A. (2016). Recent trends in worldwide abalone production. Journal of Shellfish Research, 35(3), 581-583. https://doi.org/10.2983/035.035.0302
  • Duncan, P.F., Andalecio, M.N., Peralta, E., Laureta, L., Hidalgo, A.R., Napata, R. (2009). Evaluation of production technology, product quality and market potential for the development of bivalve mollusc aquaculture in the Philippines. Australian Centre for International Agricultural Research (ACIAR), 193 pp.
  • Edun, O.M., Akinrotimi, O.A., Makinde, O.O. (2016). Seasonal changes of microbial load in some sea foos from Baguma and Ekerekana Creeks, Niger Delta, Nigeria. Peertechz Journal of Environmental Science and Toxicology, 1(1), 001-007. https://doi.org/10.17352/aest.000001
  • Encena, II V.C., Bayona, N.C. (2010). Farming of the tropical abalone Haliotis asinina. Aquaculture Extension Manual, 49, 1-24.
  • European Communities (EC). (2007). Commission regulation no. 1441/2007 of December 2007 amending regulation (EC) no. 2073/2005 on microbiological criteria for foodstuffs. Official Journal of the European Union, 322, 12-29. http://data.europa.eu/eli/reg/2007/1441/oj
  • Florini, S., Shahsavari, E., Ngo, T., Aburto-Medina, A., Smith, D.J., Ball, A.S. (2020). Factors influencing the concentration of fecal coliforms in oysters in the River Blackwater Estuary, UK. Water, 12, 1-12. https://doi.org/10.3390/w12041086
  • Food Safety Inspection Service (FSIS) (2014). Foodborne Illness: What Consumers Need to Know. Washington, DC: United States Department of Agriculture. Retrieved from http://www.fsis.usda.gov/wps/portal/fsis/topics/food-safety-education/get-answers/food-safety-fact-sheets/foodborne-illness-and-disease/foodborne-illness-what-consumers-need-to-know/CT_Index on 16 Oct 2020.
  • Food and Agriculture Organization (FAO) (2016). The State of World Fisheries and Aquaculture. Contributing to food security and nutrition for all. Rome. 200 pp. ISBN 978-92-5-109185-2.
  • Food and Agriculture Organization (FAO), World Health Organization (WHO) (2020). Risk assessment tools for Vibrio parahaemolyticus and Vibrio vulnificus associated with seafood. Microbiological Risk Assessment Series No. 20. Rome.
  • Food and Agriculture Organization (FAO), World Health Organization (WHO) (2011). Risk assessment of Vibrio parahaemolyticus in seafood: Interpretative summary and Technical report. Microbiological Risk Assessment Series, No 16, Rome.
  • Food and Agriculture Organization (FAO), World Health Organization (WHO) (2005). Risk assessment of Vibrio vulnificus in raw oysters: Interpretative summary and Technical report. Microbiological Risk Assessment Series, No 8, Rome.
  • Food Drugs Administration (FDA). (2009). National Shellfish Sanitation Program guide for the control of molluscan shellfish 2007 revisions. Retrieved from http://www.cfsan.fda.gov/;ear/nss4-toc.html (accessed 10.11 2017)
  • Gnanambal, K., Patterson, J. (2005). Biochemical and microbiological quality of frozen fishes available in Tuticorin supermarkets. Fishery Technology, 42(1), 83-84.
  • Handlinger, J.J., Donachie, C.L., Gabor, L., Taylor, D. (2005). Bacterial infection in Tasmanian farmed abalone: Causes, pathology, farm factors and control options. Diseases of Asian Aquaculture, 5, 289-299.
  • International Commission of Microbiological Specification for Food (ICMSF). (1986). Microorganisms in foods. Vol. 1: Their significance and methods of enumeration. 2nd edition, Thatcher F. S., Clark D. S. (eds), University of Toronto Press, Toronto, ON, Canada, pp. 19-30, 181.
  • Iwamoto, M., Ayers, T., Mahon, B.E., Swerdlow, D.L. (2010). Epidemiology of seafood-associated infections in the United States. Clinical Microbiology Reviews, 23(2), 399-411. https://doi.org/10.1128/CMR.00059-09
  • Kua, B.C., Ramly, R., Devakie, M., Groman, D., Berthe, C.J.F. (2011). Investigating a mortality in hatchery cultured tropical abalone, Haliotis asinina Linnaeus, 1758 in Malaysia. Diseases of Asian Aquaculture, 7, 103-109.
  • Lameira Silva, O.L., Veríssimo S.M.M., da Rosa, A.M.B.P., Iguchi, B.Y., Nunes, E.D.S.C.D.L., Moraes, C.M.D., Cordeiro, C.A.M., Xavier, D.D.A., Pinto, A.S.O., Peixoto Joele, M.R.S., Brito, J.D.S., Juen, L., Rocha, R.M.D. (2020). Effects of environmental factors on microbiological quality of oyster farming in Amazon estuaries. Aquaculture Reports, 18, 1-10. https://doi.org/10.1016/j.aqrep.2020.100437
  • Lee, K.K., Liu, P.C., Chen, Y.C., Huang, C.Y. (2001). The implication of ambient temperature with the outbreak of vibriosis in cultured small abalone Haliotis diversicolor supertaxa Lischke. Journal of Thermal Biology, 26, 585-587. https://doi.org/10.1016/S0306-4565(01)00004-3
  • Lee, M. J., Lee, J. J., Chung, H. Y., Choi, S. H., Kim, B. S. (2016). Analysis of microbiota on abalone (Haliotis discus hannai) in South Korea for improved product management. International journal of food microbiology, 234, 45-52. https://doi.org/10.1016/j.ijfoodmicro.2016.06.032
  • Mabuhay-Omar, J.A., Cayabo, G.D.B., Nuñala, I.J.P., Habal, S.E., Creencia, L.A. (2019). Microbial and microparasite abundance in cage-cultured abalone Haliotis asinina. Journal of Shellfish Research, 38(2), 405-411. https://doi.org/10.2983/035.038.0223
  • Martínez, O., Rodríguez-Calleja, J.M., Santos, J.A., Otero, A., García-López, M.L. (2009). Foodborne and indicator bacteria in farmed molluscan shellfish before and after depuration. Journal of Food Protection, 72(7), 1443-1449. https://doi.org/10.4315/0362-028x-72.7.1443
  • Philippine National Standards - Bureau of Agriculture and Fisheries Product standards (PNS-BAFPS). (2011). Philippine National Standards - Bureau of Agriculture and Fisheries Product Standards for Live and Raw Molluscs. PNS/BAFPS 89:2011, ICS 67.120.30.
  • Pitchon, D., Cudennec, B., Huchette, S., Djediat, C., Renault, T., Paillard, C., Auzoux-Bordenave, S. (2013). Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures. Cytotechnology, 65, 759-772. https://doi.org/10.1007/s10616-013-9583-1
  • Romalde, J.L., Diéguez, A.l., Lasa, A., Balboa, S. (2014). New Vibrio species associated to molluscan microbiota: a review. Frontiers in Microbiology, 4, 1-11. https://doi.org/10.3389/fmicb.2013.00413
  • Sanders, E.R. (2012). Aseptic laboratory techniques: Plating methods. Journal of Visualized Experiments, 63, 1-18. https://doi.org/10.3791/3064
  • Sanjee, S.A., Karim, M.E. (2016). Microbiological quality assessment of frozen fish and fish processing materials from Bangladesh. International Journal of Food Science, 1-6. https://doi.org/10.1155/2016/8605689
  • Santiago, C.H.S., Mabuhay-Omar, J.A. (2019). Isolation and characterization of antimicrobial-producing bacteria from the donkey's ear abalone Haliotis asinina, Journal of Shellfish Research, 38(2), 413-416. https://doi.org/10.2983/035.038.0224
  • Sorio, J.C., Peralta, J.P. (2018). Microbiological quality of oyster (Crassostrea iredalei) in selected production areas in Dumangas, Iloilo, Philippines. AACL Bioflux, 11(2), 319-326.
  • Suleria, H.A.R., Masci, P.P., Gobe, G.C., Osborne, S.A. (2017). Therapeutic potential of abalone and status of bioactive molecules: A comprehensive review. Critical Reviews in Food Science and Nutrition, 57(8), 1742-1748. https://doi.org/10.1080/10408398.2015.1031726
  • Surtida, A.P. (2000). Abalone. SEAFDEC Asian Aquaculture, 22(4), 14-16. World Health Organization (WHO). (2001). Water quality: Guidelines, standards and health. IWA Publishing, London, UK. ISBN: 1900222 28 0
  • World Health Organization (WHO). (1996). Weekly Epidemiological Record. 73, 201-208.
  • Yagoub, S.O., Ahmed, T.M. (2003). Pathogenic microorganisms in freshwater samples collected from Khartoum central market. Sudan Journal of Veterinary Science and Animal Husbandry, 43, 32–37.
There are 41 citations in total.

Details

Primary Language English
Subjects Hydrobiology
Journal Section Research Articles
Authors

Jhonamie Mabuhay-omar 0000-0002-8384-4449

Genese Divine B. Cayabo 0000-0001-8038-0485

Lota A. Creencıa 0000-0002-8586-8604

Project Number 315
Publication Date April 1, 2021
Submission Date August 6, 2020
Published in Issue Year 2021

Cite

APA Mabuhay-omar, J., Cayabo, G. D. B., & Creencıa, L. A. (2021). Bacteriological quality of cage-cultured abalone Haliotis asinina. Aquatic Research, 4(2), 151-159. https://doi.org/10.3153/AR21012

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