Research Article
BibTex RIS Cite

PATHOGENICITY AND PATHOLOGY OF Streptococcus agalactiae IN CHALLENGED MOZAMBIQUE TILAPIA Oreochromis mossambicus (PETERS 1852) JUVENILES

Year 2019, , 182 - 190, 01.10.2019

Thangapalam Jawahar Abraham , Meshram Supradhnya Namdeo Harresh Adıkesavalu Sayani Banerjee

https://doi.org/10.3153/AR19017

Abstract

Streptococcosis
is one of the most important bacterial diseases of tilapia. The present study
assessed the histopathological changes induced by Streptococcus agalactiae challenge in the brain, kidney, spleen,
and liver of Oreochromis mossambicus.
When challenged intraperitoneally at 107-108 cells/fish, S. agalactiae strains (TKT1
and TBT2) caused 40-100% mortalities in O. mossambicus. The LD50 values of S. agalactiae TKT1 and TBT2 strains were
1.60×107 and 7.33×107 cells/fish, respectively.
Histological sections of the challenged O.
mossambicus brain exhibited meningoencephalitis, marginated haemocytes,
extensive haemorrhages, oedema and neurons with marginated nuclei. The kidney
of challenged tilapia showed glomerulopathy, dilation of Bowman’s capsule,
nephritis, haematopoietic tissue necrosis, melanization and granulomatous-like
lesions. The spleen was characterized by extensive melanomacrophage
aggregation, necrosis and vasodilation. The liver had dilated and ruptured
blood capillary, melanization and disintegrated tissue. The intrahepatic
exocrine pancreatic tissue was disintegrated. Our results demonstrated that S. agalactiae caused a systemic
infection and meningoencephalitis in the Mozambique tilapia juveniles.

Keywords

Oreochromis mossambicus Streptococcus agalactiae Meningoencephalitis Pathogenicity Granulomatous-like lesions

Supporting Institution

ICAR, New Delhi

Project Number

Grant F. 10(12)/2012–EPD dated 23.03.2012

Thanks

The authors thank the Vice-Chancellor, West Bengal University of Animal and Fishery Sciences, Kolkata, India for providing necessary infrastructure facility to carry out the work.

References

  • Adikesavalu, H., Patra, A., Banerjee, S., Sarkar, A., Abraham, T.J. (2015). Phenotypic and molecular characterization and pathology of Flectobacillus roseus causing flectobacillosis in captive held carp Labeo rohita (Ham.) fingerlings. Aquaculture, 439, 60-65. https://doi.org/10.1016/j.aquaculture.2014.12.036
  • Adikesavalu, H., Banerjee, S., Patra, A., Abraham, T.J. (2017). Meningoencephalitis in farmed mono-sex Nile tilapia (Oreochromis niloticus L.) caused by Streptococcus agalactiae. Archives of Polish Fisheries, 25, 187-200. https://doi.org/10.1515/aopf-2017-0018
  • Al-Harbi A.H. (1996). Susceptibility of five species of tilapia to Streptococcus sp. Asian Fisheries Science, 9, 177-181.
  • Alsaid, M., Daud, H.H.M., Mustapha, N.M., Bejo, S.K., Abdelhadi, Y.M., Abuseliane, A.F., Hamdan, R.H. (2013). Pathological findings of experimental Streptococcus agalactiae infection in red hybrid tilapia (Oreochromis sp.). In Proceedings of the International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2013), (p. 70-73). Kuala Lumpur, Malaysia.
  • Amal, M.N.A., Zamri-Saad, M. (2011). Streptococcosis in tilapia (Oreochromis niloticus): a review. Pertanika Journal of Tropical Agricultural Science, 34, 195-206.
  • Behera, B.K. Pradhan, P.K., Swaminathan, T.R., Sood, N., Prasenjit, P., Das, A., Verma, D.K., Kumar, R., Yadav M.K., Dev, A.K., Parida, P.K., Das, B.K., Lal, K.K., Jena, J.K. (2018). Emergence of Tilapia Lake Virus associated with mortalities of farmed Nile Tilapia Oreochromis niloticus (Linnaeus 1758) in India. Aquaculture, 484, 168-174. https://doi.org/10.1016/j.aquaculture.2017.11.025
  • Boyd, C.E. (2004). Farm-level Issues in Aquaculture Certification: Tilapia. Report commissioned by WWF, p.29. Available at http://fisheries.tamu.edu/files/2013/09Farm-Level-Issues-in-Aquaculture-Certification-Tilapia.pdf (accessed on 19 October 2017)
  • El-Sayed, A.F.M. (2006). Tilapia culture. Wallingford: CAB International, p.277. https://doi.org/10.1079/9780851990149.0000
  • Evans, J.J., Klesius, P.H., Gilbert, P.M., Shoemaker, C.A., Al-Sarawi, M.A., Landsberg, J., Duremdez, R., Al-Marzouk, A., Al-Zenki, S. (2002). Characterization of beta-hemolytic Group B Streptococcus agalactiae in cultured gilthead seabream, Sparus auratus (L.) and wild mullet, Liza klunzingeri (Day), in Kuwait. Journal of Fish Diseases, 5, 505-513. https://doi.org/10.1046/j.1365-2761.2002.00392.x
  • Evans, J.J., Klesius, P.H., Shoemaker, C.A. (2006). An overview of streptococcus in warm-water fish. Aquaculture Health International, 7, 10-14.
  • Eyngor, M., Zamostiano, R., Tsofack, J.E.K., Berkowitz, A., Bercovier, H., Tinman, S., Lev, M., Hurvitz, A., Galeotti, M., Bacharach, E., Eldara, A. (2014). Identification of a novel RNA virus lethal to tilapia. Journal of Clinical Microbiology, 52(12), 4137-4146. https://doi.org/10.1128/JCM.00827-14
  • FAO (2017). Outbreaks of Tilapia lake virus (TiLV) threaten the livelihoods and food security of millions of people dependent on tilapia farming. GIEWS Special Alert No: 338 - Global. Available at: http://www.fao.org/3/a-i7326e.pdf (accessed on 20 October 2017).
  • FAO (2018). The State of World Fisheries and Aquaculture 2018 - Meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO.
  • Galhardo, L. (2010). Teleost welfare: Behavioural, cognitive and physiological aspects in Oreochromis mossambicus. PhD thesis. Porto, Portugal: Instituto De Ciências Biomédicas Abel Salazar, Universidade Do Porto, p.215
  • Gültepe, N., Bilen, S., Yilmaz, S., Güroy, D., Aydin, S. (2014). Effects of herbs and spice on health status of tilapia (Oreochromis mossambicus) challenged with Streptococcus iniae. Acta Veterinaria Brno, 83, 125-131. https://doi.org/10.2754/avb201483020125
  • Hernández, E., Figueroa, J., Iregui, C. (2009). Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study. Journal of Fish Diseases, 32, 247-252. https://doi.org/10.1111/j.1365-2761.2008.00981.x
  • Iregui, C., Barato, P., Rey, A., Vasquez, G., Verján, N. (2014). Epidemiology of Streptococcus agalactiae and Streptococcosis in tilapia fish. In iConcept Press Ltd (ed.), Epidemiology: Theory, Research and Practice. 1st edn. Chapter 10. Hong Kong: iConcept Press Ltd, pp.18.
  • Iregui, C., Comas, J., Vásquez, G.M., Verján, N. (2016). Experimental early pathogenesis of Streptococcus agalactiae infection in red tilapia Oreochromis spp. Journal of Fish Diseases, 39, 205-215. https://doi.org/10.1111/jfd.12347
  • Klesius, P.H., Shoemaker, C.A., Evans, J.J. (2008). Streptococcus: A worldwide fish health problem. In Proceedings of the 8th International Symposium on Tilapia in Aquaculture. (p. 83-107). Cairo, Egypt.
  • Li, Y.W., Liu, L., Huang, P.R., Fang, W., Luo, Z.P., Peng, H.L., Wang, X.Y., Li, A.X. (2014). Chronic streptococcosis in Nile tilapia, Oreochromis niloticus (L.) caused by Streptococcus agalactiae. Journal of Fish Diseases, 37, 757-763. https://doi.org/10.1111/jfd.12146
  • Mishra, A., Nam, G-H., Gim, J-A., Lee, H-E., Jo, A., Kim, H-S. (2018). Current challenges of streptococcus infection and effective molecular, cellular, and environmental control methods in aquaculture. Molecules and Cells, 41(6), 495-505.
  • Mukhi, S.K. (1999). Streptococcal Infection in Cultured Tilapia Oreochromis mossambicus (Peters). Master's thesis. Mumbai, India: Central Institute of Fisheries Education, p.66.
  • Ndong, D., Chen, Y.Y., Lin, Y.H., Vaseeharan, B., Chen, J.C. (2007). The immune response of tilapia Oreochromis mossambicus and its susceptibility to Streptococcus iniae under stress in low and high temperatures. Fish and Shellfish Immunology, 22(6), 686-694. https://doi.org/10.1016/j.fsi.2006.08.015
  • Pulido, E., Iregui, C., Figueroa, J., Klesius, P.H. (2004). Estreptococosis en tilapias (Oreochromis spp.) cultivadas en Colombia. Revista Aquatic, 20, 97-106.
  • Reed, L.J., Muench, H. (1938). A simple method of estimating fifty percent endpoints. American Journal of Epidemiology, 27(3), 493-497. https://doi.org/10.1093/oxfordjournals.aje.a118408
  • Roberts, R.J. (2012). Fish Pathology (4th ed.). Wiley-Blackwell, UK. p. 590. https://doi.org/10.1002/9781118222942
  • Sugunan, V.V. (1995). Exotic Fishes and their Role in Reservoir Fisheries in India. FAO Fisheries Technical Paper No. 345. Rome: FAO, p.423.
  • Trewavas, E. (1983). Tilapine fishes of the genera Sarotherodon, Oreochromis and Danakilia, London, UK: British Museum (Natural History). https://doi.org/10.5962/bhl.title.123198
  • Tung, M.C., Chen, S.C., Tsai, S.S. (1985). General septicemia of streptococcal infection in cage-cultured Tilapia mossambica in southern Taiwan. COA Fisheries Series No. 4, Fish Disease Research, VII: 95-105.
  • Wang, K.Y., Chen, D.F., Huang, L.Y., Lian, H., Wang, J., Xiao, D., Geng, Y., Yang, Z.X., Lai, W.M. (2013). Isolation and characterization of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus in China. African Journal of Microbiology Research, 7, 317-323. https://doi.org/10.5897/AJMR12.1207
  • Yilmaz, S., Ergün, S., Soytaş, N. (2013). Herbal supplements are useful for preventing streptococcal disease during first-feeding of tilapia fry, Oreochromis mossambicus. Israeli Journal of Aquaculture Bamidgeh, 65, 833.
  • Zamri-Saad, M., Amal, M.N.A., Siti-Zahrah, A. (2010). Pathological changes in red tilapias (Oreochromis spp.) naturally infected by Streptococcus agalactiae. Journal of Comparative Pathology, 143, 227-229. https://doi.org/10.1016/j.jcpa.2010.01.020
  • Zamri-Saad, M., Amal, M.N.A., Siti-Zahrah, A., Zulkafli, A.R. (2014). Control and prevention of streptococcosis in cultured tilapia in Malaysia: A review. Pertanika Journal of Tropical Agricultural Science, 37(4), 389-410.
  • Zlotkin, A., Hershko, H., Eldar, A. (1998). Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Applied and Environmental Microbiology, 64, 4065-4067.

Year 2019, , 182 - 190, 01.10.2019

Thangapalam Jawahar Abraham , Meshram Supradhnya Namdeo Harresh Adıkesavalu Sayani Banerjee

https://doi.org/10.3153/AR19017

Abstract

Project Number

Grant F. 10(12)/2012–EPD dated 23.03.2012

References

  • Adikesavalu, H., Patra, A., Banerjee, S., Sarkar, A., Abraham, T.J. (2015). Phenotypic and molecular characterization and pathology of Flectobacillus roseus causing flectobacillosis in captive held carp Labeo rohita (Ham.) fingerlings. Aquaculture, 439, 60-65. https://doi.org/10.1016/j.aquaculture.2014.12.036
  • Adikesavalu, H., Banerjee, S., Patra, A., Abraham, T.J. (2017). Meningoencephalitis in farmed mono-sex Nile tilapia (Oreochromis niloticus L.) caused by Streptococcus agalactiae. Archives of Polish Fisheries, 25, 187-200. https://doi.org/10.1515/aopf-2017-0018
  • Al-Harbi A.H. (1996). Susceptibility of five species of tilapia to Streptococcus sp. Asian Fisheries Science, 9, 177-181.
  • Alsaid, M., Daud, H.H.M., Mustapha, N.M., Bejo, S.K., Abdelhadi, Y.M., Abuseliane, A.F., Hamdan, R.H. (2013). Pathological findings of experimental Streptococcus agalactiae infection in red hybrid tilapia (Oreochromis sp.). In Proceedings of the International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2013), (p. 70-73). Kuala Lumpur, Malaysia.
  • Amal, M.N.A., Zamri-Saad, M. (2011). Streptococcosis in tilapia (Oreochromis niloticus): a review. Pertanika Journal of Tropical Agricultural Science, 34, 195-206.
  • Behera, B.K. Pradhan, P.K., Swaminathan, T.R., Sood, N., Prasenjit, P., Das, A., Verma, D.K., Kumar, R., Yadav M.K., Dev, A.K., Parida, P.K., Das, B.K., Lal, K.K., Jena, J.K. (2018). Emergence of Tilapia Lake Virus associated with mortalities of farmed Nile Tilapia Oreochromis niloticus (Linnaeus 1758) in India. Aquaculture, 484, 168-174. https://doi.org/10.1016/j.aquaculture.2017.11.025
  • Boyd, C.E. (2004). Farm-level Issues in Aquaculture Certification: Tilapia. Report commissioned by WWF, p.29. Available at http://fisheries.tamu.edu/files/2013/09Farm-Level-Issues-in-Aquaculture-Certification-Tilapia.pdf (accessed on 19 October 2017)
  • El-Sayed, A.F.M. (2006). Tilapia culture. Wallingford: CAB International, p.277. https://doi.org/10.1079/9780851990149.0000
  • Evans, J.J., Klesius, P.H., Gilbert, P.M., Shoemaker, C.A., Al-Sarawi, M.A., Landsberg, J., Duremdez, R., Al-Marzouk, A., Al-Zenki, S. (2002). Characterization of beta-hemolytic Group B Streptococcus agalactiae in cultured gilthead seabream, Sparus auratus (L.) and wild mullet, Liza klunzingeri (Day), in Kuwait. Journal of Fish Diseases, 5, 505-513. https://doi.org/10.1046/j.1365-2761.2002.00392.x
  • Evans, J.J., Klesius, P.H., Shoemaker, C.A. (2006). An overview of streptococcus in warm-water fish. Aquaculture Health International, 7, 10-14.
  • Eyngor, M., Zamostiano, R., Tsofack, J.E.K., Berkowitz, A., Bercovier, H., Tinman, S., Lev, M., Hurvitz, A., Galeotti, M., Bacharach, E., Eldara, A. (2014). Identification of a novel RNA virus lethal to tilapia. Journal of Clinical Microbiology, 52(12), 4137-4146. https://doi.org/10.1128/JCM.00827-14
  • FAO (2017). Outbreaks of Tilapia lake virus (TiLV) threaten the livelihoods and food security of millions of people dependent on tilapia farming. GIEWS Special Alert No: 338 - Global. Available at: http://www.fao.org/3/a-i7326e.pdf (accessed on 20 October 2017).
  • FAO (2018). The State of World Fisheries and Aquaculture 2018 - Meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO.
  • Galhardo, L. (2010). Teleost welfare: Behavioural, cognitive and physiological aspects in Oreochromis mossambicus. PhD thesis. Porto, Portugal: Instituto De Ciências Biomédicas Abel Salazar, Universidade Do Porto, p.215
  • Gültepe, N., Bilen, S., Yilmaz, S., Güroy, D., Aydin, S. (2014). Effects of herbs and spice on health status of tilapia (Oreochromis mossambicus) challenged with Streptococcus iniae. Acta Veterinaria Brno, 83, 125-131. https://doi.org/10.2754/avb201483020125
  • Hernández, E., Figueroa, J., Iregui, C. (2009). Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study. Journal of Fish Diseases, 32, 247-252. https://doi.org/10.1111/j.1365-2761.2008.00981.x
  • Iregui, C., Barato, P., Rey, A., Vasquez, G., Verján, N. (2014). Epidemiology of Streptococcus agalactiae and Streptococcosis in tilapia fish. In iConcept Press Ltd (ed.), Epidemiology: Theory, Research and Practice. 1st edn. Chapter 10. Hong Kong: iConcept Press Ltd, pp.18.
  • Iregui, C., Comas, J., Vásquez, G.M., Verján, N. (2016). Experimental early pathogenesis of Streptococcus agalactiae infection in red tilapia Oreochromis spp. Journal of Fish Diseases, 39, 205-215. https://doi.org/10.1111/jfd.12347
  • Klesius, P.H., Shoemaker, C.A., Evans, J.J. (2008). Streptococcus: A worldwide fish health problem. In Proceedings of the 8th International Symposium on Tilapia in Aquaculture. (p. 83-107). Cairo, Egypt.
  • Li, Y.W., Liu, L., Huang, P.R., Fang, W., Luo, Z.P., Peng, H.L., Wang, X.Y., Li, A.X. (2014). Chronic streptococcosis in Nile tilapia, Oreochromis niloticus (L.) caused by Streptococcus agalactiae. Journal of Fish Diseases, 37, 757-763. https://doi.org/10.1111/jfd.12146
  • Mishra, A., Nam, G-H., Gim, J-A., Lee, H-E., Jo, A., Kim, H-S. (2018). Current challenges of streptococcus infection and effective molecular, cellular, and environmental control methods in aquaculture. Molecules and Cells, 41(6), 495-505.
  • Mukhi, S.K. (1999). Streptococcal Infection in Cultured Tilapia Oreochromis mossambicus (Peters). Master's thesis. Mumbai, India: Central Institute of Fisheries Education, p.66.
  • Ndong, D., Chen, Y.Y., Lin, Y.H., Vaseeharan, B., Chen, J.C. (2007). The immune response of tilapia Oreochromis mossambicus and its susceptibility to Streptococcus iniae under stress in low and high temperatures. Fish and Shellfish Immunology, 22(6), 686-694. https://doi.org/10.1016/j.fsi.2006.08.015
  • Pulido, E., Iregui, C., Figueroa, J., Klesius, P.H. (2004). Estreptococosis en tilapias (Oreochromis spp.) cultivadas en Colombia. Revista Aquatic, 20, 97-106.
  • Reed, L.J., Muench, H. (1938). A simple method of estimating fifty percent endpoints. American Journal of Epidemiology, 27(3), 493-497. https://doi.org/10.1093/oxfordjournals.aje.a118408
  • Roberts, R.J. (2012). Fish Pathology (4th ed.). Wiley-Blackwell, UK. p. 590. https://doi.org/10.1002/9781118222942
  • Sugunan, V.V. (1995). Exotic Fishes and their Role in Reservoir Fisheries in India. FAO Fisheries Technical Paper No. 345. Rome: FAO, p.423.
  • Trewavas, E. (1983). Tilapine fishes of the genera Sarotherodon, Oreochromis and Danakilia, London, UK: British Museum (Natural History). https://doi.org/10.5962/bhl.title.123198
  • Tung, M.C., Chen, S.C., Tsai, S.S. (1985). General septicemia of streptococcal infection in cage-cultured Tilapia mossambica in southern Taiwan. COA Fisheries Series No. 4, Fish Disease Research, VII: 95-105.
  • Wang, K.Y., Chen, D.F., Huang, L.Y., Lian, H., Wang, J., Xiao, D., Geng, Y., Yang, Z.X., Lai, W.M. (2013). Isolation and characterization of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus in China. African Journal of Microbiology Research, 7, 317-323. https://doi.org/10.5897/AJMR12.1207
  • Yilmaz, S., Ergün, S., Soytaş, N. (2013). Herbal supplements are useful for preventing streptococcal disease during first-feeding of tilapia fry, Oreochromis mossambicus. Israeli Journal of Aquaculture Bamidgeh, 65, 833.
  • Zamri-Saad, M., Amal, M.N.A., Siti-Zahrah, A. (2010). Pathological changes in red tilapias (Oreochromis spp.) naturally infected by Streptococcus agalactiae. Journal of Comparative Pathology, 143, 227-229. https://doi.org/10.1016/j.jcpa.2010.01.020
  • Zamri-Saad, M., Amal, M.N.A., Siti-Zahrah, A., Zulkafli, A.R. (2014). Control and prevention of streptococcosis in cultured tilapia in Malaysia: A review. Pertanika Journal of Tropical Agricultural Science, 37(4), 389-410.
  • Zlotkin, A., Hershko, H., Eldar, A. (1998). Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Applied and Environmental Microbiology, 64, 4065-4067.
There are 34 citations in total.

Details

Primary Language English
Subjects Hydrobiology
Journal Section Research Articles
Authors

Thangapalam Jawahar Abraham 0000-0003-0581-1307

Meshram Supradhnya Namdeo 0000-0002-6046-9703

Harresh Adıkesavalu 0000-0002-2258-1470

Sayani Banerjee 0000-0001-6527-4481

Project Number Grant F. 10(12)/2012–EPD dated 23.03.2012
Publication Date October 1, 2019
Submission Date August 5, 2019
Published in Issue Year 2019

Cite

APA Abraham, T. J., Namdeo, M. S., Adıkesavalu, H., Banerjee, S. (2019). PATHOGENICITY AND PATHOLOGY OF Streptococcus agalactiae IN CHALLENGED MOZAMBIQUE TILAPIA Oreochromis mossambicus (PETERS 1852) JUVENILES. Aquatic Research, 2(4), 182-190. https://doi.org/10.3153/AR19017

16291

is licensed under a CreativeCommons Attribtion-ShareAlike 4.0 International Licence 14628   1325927040

Diamond Open Access refers to a scholarly publication model in which journals and platforms do not charge fees to either authors or readers.

Open Access Statement:

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

Archiving Policy:

Archiving is done according to TÜBİTAK ULAKBİM "DergiPark" publication policy (LOCKSS).