Research Article
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EVALUATING THE ESSENTIAL AND NON-ESSENTIAL METAL REMEDIATION EFFICIENCY OF Chlorella vulgaris, AND PHOTOSYNTHETIC GENE EXPRESSION LEVEL CHANGES DURING THE PROCESS

Year 2019, , 134 - 142, 01.07.2019

Tuğba Şentürk , Muhammet Burak Batır , Çisil Çamlı Şükran Yıldız

https://doi.org/10.3153/AR19011

Abstract

Algal
populations hold great potential for
encountering water pollution problem due to their remediation abilities. Thus,
using them for removing the pollutants stands as a powerful approach. However, it is crucial to investigate the
negative effects of these pollutants on algal populations as well as
understanding the removal capacity of these populations in order to benefit
their abilities. In the present study, Chlorella
vulgaris was used as a candidate for
metal removal. Besides the remediation capacity for certain essential (Cu2+,
Zn2+, Co2+, Mn2+, Mo6+) and
non-essential (Cd2+, Pb2+, Sn4+, Ba2+,
As5+) metals, chlorophyll and carbohydrate contents, and
photosynthetic gene (psaB, Photosystem I reaction center protein subunit B) expression levels were also evaluated.  Results
indicated that remediation efficiency of C.
vulgaris for essential metals was
Cu>Co>Zn>Mo>Mn and for non-essential metals was
Sn>Pb>Ba>Cd>As, respectively. It was also observed that psaB
expression was increased after the essential and non-essential metal treatment.
It can be concluded that C. vulgaris can be used as a bioindicator for Mnand Aswhile
it is also suitable to be used for metal removal. 

Keywords

Bioremediation Phytoremediation Metal pollution Metal removal Algal removal

Supporting Institution

Manisa Celal Bayar University

Project Number

FEF 2015–154

References

  • Aranda, P.S., LaJoie, D.M., Jorcyk, C.L. (2012). Bleach Gel: A simple agarose gel for analyzing RNA quality. Electrophoresis. 33(2), 366–369.
  • Bajguz, A. (2011). Suppression of Chlorella vulgaris growth by cadmium, lead, and copper stress and its restoration by endogenous brassinolide. Archives of Environmental Contamination and Toxicology, 60(3), 406-416.
  • Çetinkaya, Dönmez, G., Aksu, Z., Öztürk, A., Kutsal, T. (1999). A comparative study on heavy metal biosorption characteristics of some algae. Process Biochemistry, 34(1), 885-892. De Philippis, R., Paperi, R., Sili, C. (2007). Heavy metal sorption by released polysaccharides and whole cultures of two exopolysaccharide-producing cyanobacteria. Biodegradation, 18(2), 181-187.
  • Fayed, S.E., Abdel-Shafy, H.I., Khalifa, N.M. (1983). Accumulation of Cu, Zn, Cd, and Pb by Scenedesmus obliquus under nongrowth conditions. Environment International, 9(5), 409-413.
  • Franklin, N.M., Stauber, J.L., Markich, S.J., Lim, R.P. (2000). pH-dependent toxicity of copper and uranium to a tropical freshwater alga (Chlorella sp.). Aquatic Toxicology, 48(2-3), 275-289.
  • Freundlich, H. (1907). Über die adsorption in lösungen. Zeitschrift Für Physikalische Chemie, 57(1), 385-470.
  • Hong, K.S., Lee, H.M., Bae, J.S., Ha, M.G., Jin, J.S., Hong, T.E., Kim, J.P., Jeong, E.D. (2011). Removal of heavy metal ions by using calcium carbonate extracted from starfish treated by protease and amylase. Journal of Analytical Science and Technology, 2(2), 75-82.
  • Islam, E., Yang, X.E., He, Z.L., Mahmood, Q. (2007). Assessing potential dietary toxicity of heavy metals in selected vegetables and food crops. Journal of Zhejiang University-Science B, 8(1), 1-13.
  • Khan, M.A., Rao, R.A.K., Ajmal, M. (2008). Heavy metal pollution and its control through nonconventional adsorbents (1998-2007): a review. Journal of International Environmental Application and Science, 3(2), 101-141.
  • Knauer, K., Behra, R., & Sigg, L. (1997). Effects of free cu2+ and zn2+ ions on growth and metal accumulation in freshwater algae. Environmental Toxicology and Chemistry, 16(2), 220-229.
  • König-Peter, A., Ferenc, K., Felinger, A., Pernyeszi, T. (2015). Biosorption characteristics of Spirulina and Chlorella cells for the accumulation of heavy metals. Journal of the Serbian Chemical Society, 80(3), 407-419.
  • Langmuir, I. (1916). The constitution and fundamental properties of solids and liquids. Part I. Solids. Journal of the American Chemical Society. 1916, 38(11), 2221-2295.
  • López-Suárez, C., Castro-Romero, J., González-Rodrı́guez, M., González-Soto, E., Pérez-Iglesias, J., Seco-Lago, H.M., Fernández-Solís, J.M. (2000). Study of the parameters affecting the binding of metals in solution by Chlorella vulgaris. Talanta, 50(6), 1313-1318.
  • Lu, C., Chau, C., Zhang, J. (2000). Acute toxicity of excess mercury on the photosynthetic performance of Cyanobacterium, S. platensis – assessment by chlorophyll fluorescence analysis. Chemosphere, 41(1-2), 191-196.
  • Mediouni, C., Benzarti, O., Tray, B., Habib Ghorbel, M., Jemal, F. (2006). Cadmium and Copper Toxicity for Tomato Seedlings, EDP Sci. Agronomy for Sustainable Development. 26(4), 227-232.
  • Mehta, S.K., Gaur, J.P. (2005). Use of algae for removing heavy metal ions from wastewater: progress and prospects. Critical Reviews Biotechnology, 25(3), 113-152.
  • Parsons, T.R., Strickland, J.D.H. (1965). Discussion of spectrophotometric determination of marine plant pigments, with revised equations for ascertaining chlorophylls and carotenoids. Journal of Marine Research, 21(2), 155-163.
  • Pfaffl, M.W. (2004). Quantification Strategies in Real-Time PCR Content of Chapter 3: Quantification Strategies in Real-Time PCR, In: In A–Z of Quantitative PCR. Bustin Press., California, USA., 89-112 pp.
  • Provasoli, L. (1958). Nutrition and ecology of protozoa and algae. Annual Review of Microbiology, 12(1), 279-308.
  • Qian, H., Li, J., Sun, L., Chen, W., Sheng, G.D, Liu, W., Fu, Z. (2009). Combined effect of copper and cadmium on chlorella vulgaris growth and photosynthesis-related gene transcription. Aquatic Toxicology, 94(1), 56-61.
  • Rai, L.C., Gaur, J.P., Kumar, H.D. (1981). Phycology and heavy-metal pollution. Biologycal Reviews, 56(2), 99-151.
  • Rachlin, J.W., Grosso, A. (1993). The growth response of the green alga Chlorella vulgaris to combined divalent cation exposure. Archives of Environmental Contamination and Toxicology, 24(1), 16-20.
  • Raven, J.A., Evans, M.C.W., Korb, R.E. (1999). The role of trace metals in photosynthetic electron transport in o2-evolving organisms. Photosynthesis Research, 60(2), 111-150.
  • Shioi, Y., Tamai, H., Sasa, T. (1978). Inhibition of photosystem II in the green alga Ankistrodesmus falcatus by copper. Physiology Plantarum, 44(4), 434-438.
  • Skoog, D., Holler, F., Nieman, T., Kılıç, E., Köseoğlu, F. (2000). Enstrümantal Analiz ilkeleri. Ankara, Turkey, Bilim Press., 1038 pp.
  • Soldo, D., Hari, R., Sigg, L., Behra, R. (2005). Tolerance of Oocystis nephrocytioides to copper: Intracellular distribution and extracellular complexation of copper. Aquatic Toxicology, 71(4), 307-317.
  • Stanier, R.Y., Deruelles, J., Rippka, R., Herdman, M., Waterbury, J.B. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology. 111(1), 1-61.
  • Suresh Kumar, K., Hans-Uwe, D., Eun-Ji, W., Jae-Seong, L., Kyung- Hoon, S. (2015). Microalgae – a promising tool for heavy metal remediation. Ecotoxicology and Environmental Safety, 113(1), 329-352.
  • Wang, J., Chen, C. (2009). Biosorbents for Heavy Metals Removal and Their Future. Biotechnology Advances, 27, 195-226.

Year 2019, , 134 - 142, 01.07.2019

Tuğba Şentürk , Muhammet Burak Batır , Çisil Çamlı Şükran Yıldız

https://doi.org/10.3153/AR19011

Abstract

Project Number

FEF 2015–154

References

  • Aranda, P.S., LaJoie, D.M., Jorcyk, C.L. (2012). Bleach Gel: A simple agarose gel for analyzing RNA quality. Electrophoresis. 33(2), 366–369.
  • Bajguz, A. (2011). Suppression of Chlorella vulgaris growth by cadmium, lead, and copper stress and its restoration by endogenous brassinolide. Archives of Environmental Contamination and Toxicology, 60(3), 406-416.
  • Çetinkaya, Dönmez, G., Aksu, Z., Öztürk, A., Kutsal, T. (1999). A comparative study on heavy metal biosorption characteristics of some algae. Process Biochemistry, 34(1), 885-892. De Philippis, R., Paperi, R., Sili, C. (2007). Heavy metal sorption by released polysaccharides and whole cultures of two exopolysaccharide-producing cyanobacteria. Biodegradation, 18(2), 181-187.
  • Fayed, S.E., Abdel-Shafy, H.I., Khalifa, N.M. (1983). Accumulation of Cu, Zn, Cd, and Pb by Scenedesmus obliquus under nongrowth conditions. Environment International, 9(5), 409-413.
  • Franklin, N.M., Stauber, J.L., Markich, S.J., Lim, R.P. (2000). pH-dependent toxicity of copper and uranium to a tropical freshwater alga (Chlorella sp.). Aquatic Toxicology, 48(2-3), 275-289.
  • Freundlich, H. (1907). Über die adsorption in lösungen. Zeitschrift Für Physikalische Chemie, 57(1), 385-470.
  • Hong, K.S., Lee, H.M., Bae, J.S., Ha, M.G., Jin, J.S., Hong, T.E., Kim, J.P., Jeong, E.D. (2011). Removal of heavy metal ions by using calcium carbonate extracted from starfish treated by protease and amylase. Journal of Analytical Science and Technology, 2(2), 75-82.
  • Islam, E., Yang, X.E., He, Z.L., Mahmood, Q. (2007). Assessing potential dietary toxicity of heavy metals in selected vegetables and food crops. Journal of Zhejiang University-Science B, 8(1), 1-13.
  • Khan, M.A., Rao, R.A.K., Ajmal, M. (2008). Heavy metal pollution and its control through nonconventional adsorbents (1998-2007): a review. Journal of International Environmental Application and Science, 3(2), 101-141.
  • Knauer, K., Behra, R., & Sigg, L. (1997). Effects of free cu2+ and zn2+ ions on growth and metal accumulation in freshwater algae. Environmental Toxicology and Chemistry, 16(2), 220-229.
  • König-Peter, A., Ferenc, K., Felinger, A., Pernyeszi, T. (2015). Biosorption characteristics of Spirulina and Chlorella cells for the accumulation of heavy metals. Journal of the Serbian Chemical Society, 80(3), 407-419.
  • Langmuir, I. (1916). The constitution and fundamental properties of solids and liquids. Part I. Solids. Journal of the American Chemical Society. 1916, 38(11), 2221-2295.
  • López-Suárez, C., Castro-Romero, J., González-Rodrı́guez, M., González-Soto, E., Pérez-Iglesias, J., Seco-Lago, H.M., Fernández-Solís, J.M. (2000). Study of the parameters affecting the binding of metals in solution by Chlorella vulgaris. Talanta, 50(6), 1313-1318.
  • Lu, C., Chau, C., Zhang, J. (2000). Acute toxicity of excess mercury on the photosynthetic performance of Cyanobacterium, S. platensis – assessment by chlorophyll fluorescence analysis. Chemosphere, 41(1-2), 191-196.
  • Mediouni, C., Benzarti, O., Tray, B., Habib Ghorbel, M., Jemal, F. (2006). Cadmium and Copper Toxicity for Tomato Seedlings, EDP Sci. Agronomy for Sustainable Development. 26(4), 227-232.
  • Mehta, S.K., Gaur, J.P. (2005). Use of algae for removing heavy metal ions from wastewater: progress and prospects. Critical Reviews Biotechnology, 25(3), 113-152.
  • Parsons, T.R., Strickland, J.D.H. (1965). Discussion of spectrophotometric determination of marine plant pigments, with revised equations for ascertaining chlorophylls and carotenoids. Journal of Marine Research, 21(2), 155-163.
  • Pfaffl, M.W. (2004). Quantification Strategies in Real-Time PCR Content of Chapter 3: Quantification Strategies in Real-Time PCR, In: In A–Z of Quantitative PCR. Bustin Press., California, USA., 89-112 pp.
  • Provasoli, L. (1958). Nutrition and ecology of protozoa and algae. Annual Review of Microbiology, 12(1), 279-308.
  • Qian, H., Li, J., Sun, L., Chen, W., Sheng, G.D, Liu, W., Fu, Z. (2009). Combined effect of copper and cadmium on chlorella vulgaris growth and photosynthesis-related gene transcription. Aquatic Toxicology, 94(1), 56-61.
  • Rai, L.C., Gaur, J.P., Kumar, H.D. (1981). Phycology and heavy-metal pollution. Biologycal Reviews, 56(2), 99-151.
  • Rachlin, J.W., Grosso, A. (1993). The growth response of the green alga Chlorella vulgaris to combined divalent cation exposure. Archives of Environmental Contamination and Toxicology, 24(1), 16-20.
  • Raven, J.A., Evans, M.C.W., Korb, R.E. (1999). The role of trace metals in photosynthetic electron transport in o2-evolving organisms. Photosynthesis Research, 60(2), 111-150.
  • Shioi, Y., Tamai, H., Sasa, T. (1978). Inhibition of photosystem II in the green alga Ankistrodesmus falcatus by copper. Physiology Plantarum, 44(4), 434-438.
  • Skoog, D., Holler, F., Nieman, T., Kılıç, E., Köseoğlu, F. (2000). Enstrümantal Analiz ilkeleri. Ankara, Turkey, Bilim Press., 1038 pp.
  • Soldo, D., Hari, R., Sigg, L., Behra, R. (2005). Tolerance of Oocystis nephrocytioides to copper: Intracellular distribution and extracellular complexation of copper. Aquatic Toxicology, 71(4), 307-317.
  • Stanier, R.Y., Deruelles, J., Rippka, R., Herdman, M., Waterbury, J.B. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology. 111(1), 1-61.
  • Suresh Kumar, K., Hans-Uwe, D., Eun-Ji, W., Jae-Seong, L., Kyung- Hoon, S. (2015). Microalgae – a promising tool for heavy metal remediation. Ecotoxicology and Environmental Safety, 113(1), 329-352.
  • Wang, J., Chen, C. (2009). Biosorbents for Heavy Metals Removal and Their Future. Biotechnology Advances, 27, 195-226.
There are 29 citations in total.

Details

Primary Language English
Subjects Hydrobiology
Journal Section Research Articles
Authors

Tuğba Şentürk 0000-0002-9882-0079

Muhammet Burak Batır 0000-0002-8722-5055

Çisil Çamlı 0000-0002-9641-7219

Şükran Yıldız 0000-0003-3195-2269

Project Number FEF 2015–154
Publication Date July 1, 2019
Submission Date April 15, 2019
Published in Issue Year 2019

Cite

APA Şentürk, T., Batır, M. B., Çamlı, Ç., Yıldız, Ş. (2019). EVALUATING THE ESSENTIAL AND NON-ESSENTIAL METAL REMEDIATION EFFICIENCY OF Chlorella vulgaris, AND PHOTOSYNTHETIC GENE EXPRESSION LEVEL CHANGES DURING THE PROCESS. Aquatic Research, 2(3), 134-142. https://doi.org/10.3153/AR19011

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