Research Article
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Coccidian infestation in cultured common pandora (Pagellus erythrinus)

Year 2023, , 245 - 252, 11.10.2023

Tülay Akaylı , Çiğdem Ürkü

https://doi.org/10.3153/AR23023

Abstract

Coccidia is a spore-forming obligate intracellular protozoan parasite that causes disease in many fish species. This study aimed to diagnose a parasitic disease case that affected a common pandora (Pagellus erythrinus) with a high mortality rate. The samples prepared from the internal organs of the diseased fish and the gills and muscle tissues were examined parasitologically using histological methods, a light microscope, and Transmission Electron Microscopy (TEM). No parasites or parasitic formations were found in the wet mount preparations. The presence of parasitic spores (1-1,5 x 0.3-0.7 μm) was detected towards the intestinal tissue between the intestinal microvilli of fish in the electron microscopy study. On the other hand, histological examination showed that a cystic structure full of spores (sporocyst) 550-750 μm in size developed in the abdominal muscles of the infected fish. At the same time, there were no such structures in the intestines. As a result, since the parasite spores observed in the diseased fish are very small, they settle in the cell and pass through the intestines by forming cysts in the abdominal muscles. They were identified as Coccidian sp. because of their similar morphology to those of the parasites in the Coccidian group. Coccidian infestation was detected in this fish species for the first time in this study. However, detecting the spores' entrance through the fish's intestines in the early stage and observing a small number of sporocyst structures suggest that the disease is in the development stage.

Keywords

Fish Pagellus erythrinus Parasite Coccidian TEM Histology

Supporting Institution

Istanbul University Research Projects Unit

Project Number

2637

References

  • Alvarez‐Pellitero, P., Adilla, A.S.B., Franco‐Sierra, A., & Palenzuela, O. (1995). Protozoan parasites of gilthead sea bream, Sparus aurata L., from different culture systems in Spain. Journal of Fish Diseases, 18(2), 105-115. https://doi.org/10.1111/j.1365-2761.1995.tb00268.x
  • Alvarez-Pellitero, P. (2004). Report about fish parasitic diseases. Etudes et Recherches, Options Mediterranennes. CIHEAM/FAO, Zaragoza, 103-130.
  • Başçınar, N. (2004). Dünyada su ürünleri yetiştiriciliği ve ülkemizin geleceğine bakış. Sümae Yunus Aquaculture Studies, 4(1), 6-8.
  • Bruno, D.W., Nowak, B., & Elliott, D.G. (2006). Guide to the identification of fish protozoan and metazoan parasites in stained tissue sections. Diseases of Aquatic Organisms, 70(1-2), 1-36. https://doi.org/10.3354/dao070001
  • Cascarano, M.C., Stavrakidis-Zachou, O., Mladineo, I., Thompson, K.D., Papandroulakis, N., & Katharios, P. (2021). Mediterranean aquaculture in a changing climate: temperature effects on pathogens and diseases of three farmed fish species. Pathogens, 10(9), 1205. https://doi.org/10.3390/pathogens10091205
  • Divanach, P., Kentouri, M., Charalambakis, F., Pouget, F., & Sterioti, A. (1993). Comparison of growth performance of six Mediterranean fish species reared under intensive farming conditions in Crete (Greece), in raceways with self feeders. EAS Special Publication, 285-297.
  • Dogga, S., Bartošová-Sojková, P., Lukeš, J., & Soldati-Favre, D. (2015). Phylogeny, morphology, and metabolic and invasive capabilities of epicellular fish coccidium Goussia janae. Protist, 166(6), 659-676. https://doi.org/10.1016/j.protis.2015.09.003
  • Dykova, I., & Lom, J. (1981). Fish coccidia: critical notes on life cycles, classification and pathogenicity. Journal of Fish Diseases, 4(6), 487-505.
  • FAO FishStat Plus. (2021). http://www.fao:fishery/statistics/software/fishstat/en (accessed on 30 July 2021)
  • Fioravanti, M.L., Mladineo, I., Palenzuela, O., Beraldo, P., Massimo, M., Gustinelli, A., & Sitjà-Bobadilla, A. (2020). Fish farmer's guide to combating parasite infections in European sea bass and gilthead sea bream aquaculture. A series of ParaFishControl guides to combating fish parasite infections in aquaculture. Guide 4. Sitjà-Bobadilla, A. & Bello-Gómez, E. (Eds.), e-NIPO: 833-20-104-5, pp. 29.
  • Gjurčević, E., Kužir, S., Baždarić, B., Matanović, K., Debelić, I., Marino, F., Drašner, K., & Rosenthal, B.M. (2017). New data on Eimeria dicentrarchi (Apicomplexa: Eimeriidae), a common parasite of farmed European sea bass (Dicentrarchus labrax) from the mid-eastern Adriatic. Veterinarski Arhiv, 87(1), 77-86.
  • Harding, C.R., & Frischknecht, F. (2020). The riveting cellular structures of apicomplexan parasites. Trends in Parasitology, 36(12), 979-991. https://doi.org/10.1016/j.pt.2020.09.001
  • Klaoudatos, S.D., Iakovopoulos, G., & Klaoudatos, D.S. (2004). Pagellus erythrinus: A promising candidate species for enlarging the diversity of aquaculture production. Aquaculture International, 12, 299-320. https://doi.org/10.1023/B:AQUI.0000036186.31318.4a
  • Le Breton, A.D. (1999). Mediterranean finfish pathologies: Present status and new developments in prophylactic methods. Bulletin of the European Association of Fish Pathologists, 19(6), 250-253.
  • Molnar, K. (2006). Phylum Apicomplexa fish diseases and disorders, Protozoan and Metazoan infections. 2nd edition, Woo, P.T.K. (Ed.), Canada, ISBN: 0-85199-015-0, pp.183-204. https://doi.org/10.1079/9780851990156.0183
  • Özer, A., Korniychuk, Y., Öztürk, T., Yurakhno, V., & Kornyushin, V. (2012). Parasite fauna of the Black Sea whiting, Merlangius merlangus and it’s dynamics in relation with some host factors. XI. European Multicolloquium of Parasitology (EMOP XI), 395.
  • Özer, A., Öztürk, T., Yurakhno, V., & Kornyychuk, Y. M. (2014). First report of Eimeria sardinae (Apicomplexa: Coccidia) from the Turkish coast of the Black Sea. Ege Journal of Fisheries and Aquatic Sciences, 151-153. https://doi.org/10.12714/egejfas.2014.31.3.06
  • Pasnik, D. J., Smith, S. A., & Lindsay, D. S. (2005). Intestinal coccidiosis in bluegill, Lepomis macrochirus. Journal of Parasitology, 91(4), 967-970. https://doi.org/10.1645/GE-360R.1
  • Reynolds, E.S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. The Journal of Cell Biology, 17(1), 208-212. https://doi.org/10.1083/jcb.17.1.208
  • Sitjà-Bobadilla, A., & Alvarez-Pellitero, P. (2002). Experimental transmission of Cryptosporidium molnari (Apicomplexa: Coccidia) to Giltheadsea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax). Parasitology Research, 91, 209-214. https://doi.org/10.1007/s00436-003-0945-z
  • Sitjà-Bobadilla, A., Estensoro, I., & Pérez-Sánchez, J. (2016). Immunity to gastrointestinal microparasites of fish. Developmental and Comparative Immunology, 64, 187-201. https://doi.org/10.1016/j.dci.2016.01.014 Shrestha, B. (2022). Coccidiosis in fish. lifecycle of coccidiosis, development, pathogenesis and medications. GRIN Verlag, Munich, ISBN: 978-334-661-6524.
  • Steinhagen, D., Lukes, J. & Körting, W. (1990). Ultrasuctural observations on gamegonic stages of Goussia subepithelialis (Apicomplexa, Coccidia) from common carp Cyprinus carpio. Diseases of Aquatic Organisms, 9, 31-36. https://doi.org/10.3354/dao009031
  • Watson, M.L. (1958). Staining of tissue sections for electron microscopy with heavy metals: II. Application of solutions containing lead and barium. The Journal of Cell Biology, 4(6), 727-730. https://doi.org/10.1083/jcb.4.6.727
  • Yang, R., Dorrestein, G.M., & Ryan, U. (2016). Molecular characterisation of a disseminated Cryptosporidium infection in a Koi carp (Cyprinus carpio). Veterinary Parasitology, 226, 53-56. https://doi.org/10.1016/j.vetpar.2016.06.027

Year 2023, , 245 - 252, 11.10.2023

Tülay Akaylı , Çiğdem Ürkü

https://doi.org/10.3153/AR23023

Abstract

Project Number

2637

References

  • Alvarez‐Pellitero, P., Adilla, A.S.B., Franco‐Sierra, A., & Palenzuela, O. (1995). Protozoan parasites of gilthead sea bream, Sparus aurata L., from different culture systems in Spain. Journal of Fish Diseases, 18(2), 105-115. https://doi.org/10.1111/j.1365-2761.1995.tb00268.x
  • Alvarez-Pellitero, P. (2004). Report about fish parasitic diseases. Etudes et Recherches, Options Mediterranennes. CIHEAM/FAO, Zaragoza, 103-130.
  • Başçınar, N. (2004). Dünyada su ürünleri yetiştiriciliği ve ülkemizin geleceğine bakış. Sümae Yunus Aquaculture Studies, 4(1), 6-8.
  • Bruno, D.W., Nowak, B., & Elliott, D.G. (2006). Guide to the identification of fish protozoan and metazoan parasites in stained tissue sections. Diseases of Aquatic Organisms, 70(1-2), 1-36. https://doi.org/10.3354/dao070001
  • Cascarano, M.C., Stavrakidis-Zachou, O., Mladineo, I., Thompson, K.D., Papandroulakis, N., & Katharios, P. (2021). Mediterranean aquaculture in a changing climate: temperature effects on pathogens and diseases of three farmed fish species. Pathogens, 10(9), 1205. https://doi.org/10.3390/pathogens10091205
  • Divanach, P., Kentouri, M., Charalambakis, F., Pouget, F., & Sterioti, A. (1993). Comparison of growth performance of six Mediterranean fish species reared under intensive farming conditions in Crete (Greece), in raceways with self feeders. EAS Special Publication, 285-297.
  • Dogga, S., Bartošová-Sojková, P., Lukeš, J., & Soldati-Favre, D. (2015). Phylogeny, morphology, and metabolic and invasive capabilities of epicellular fish coccidium Goussia janae. Protist, 166(6), 659-676. https://doi.org/10.1016/j.protis.2015.09.003
  • Dykova, I., & Lom, J. (1981). Fish coccidia: critical notes on life cycles, classification and pathogenicity. Journal of Fish Diseases, 4(6), 487-505.
  • FAO FishStat Plus. (2021). http://www.fao:fishery/statistics/software/fishstat/en (accessed on 30 July 2021)
  • Fioravanti, M.L., Mladineo, I., Palenzuela, O., Beraldo, P., Massimo, M., Gustinelli, A., & Sitjà-Bobadilla, A. (2020). Fish farmer's guide to combating parasite infections in European sea bass and gilthead sea bream aquaculture. A series of ParaFishControl guides to combating fish parasite infections in aquaculture. Guide 4. Sitjà-Bobadilla, A. & Bello-Gómez, E. (Eds.), e-NIPO: 833-20-104-5, pp. 29.
  • Gjurčević, E., Kužir, S., Baždarić, B., Matanović, K., Debelić, I., Marino, F., Drašner, K., & Rosenthal, B.M. (2017). New data on Eimeria dicentrarchi (Apicomplexa: Eimeriidae), a common parasite of farmed European sea bass (Dicentrarchus labrax) from the mid-eastern Adriatic. Veterinarski Arhiv, 87(1), 77-86.
  • Harding, C.R., & Frischknecht, F. (2020). The riveting cellular structures of apicomplexan parasites. Trends in Parasitology, 36(12), 979-991. https://doi.org/10.1016/j.pt.2020.09.001
  • Klaoudatos, S.D., Iakovopoulos, G., & Klaoudatos, D.S. (2004). Pagellus erythrinus: A promising candidate species for enlarging the diversity of aquaculture production. Aquaculture International, 12, 299-320. https://doi.org/10.1023/B:AQUI.0000036186.31318.4a
  • Le Breton, A.D. (1999). Mediterranean finfish pathologies: Present status and new developments in prophylactic methods. Bulletin of the European Association of Fish Pathologists, 19(6), 250-253.
  • Molnar, K. (2006). Phylum Apicomplexa fish diseases and disorders, Protozoan and Metazoan infections. 2nd edition, Woo, P.T.K. (Ed.), Canada, ISBN: 0-85199-015-0, pp.183-204. https://doi.org/10.1079/9780851990156.0183
  • Özer, A., Korniychuk, Y., Öztürk, T., Yurakhno, V., & Kornyushin, V. (2012). Parasite fauna of the Black Sea whiting, Merlangius merlangus and it’s dynamics in relation with some host factors. XI. European Multicolloquium of Parasitology (EMOP XI), 395.
  • Özer, A., Öztürk, T., Yurakhno, V., & Kornyychuk, Y. M. (2014). First report of Eimeria sardinae (Apicomplexa: Coccidia) from the Turkish coast of the Black Sea. Ege Journal of Fisheries and Aquatic Sciences, 151-153. https://doi.org/10.12714/egejfas.2014.31.3.06
  • Pasnik, D. J., Smith, S. A., & Lindsay, D. S. (2005). Intestinal coccidiosis in bluegill, Lepomis macrochirus. Journal of Parasitology, 91(4), 967-970. https://doi.org/10.1645/GE-360R.1
  • Reynolds, E.S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. The Journal of Cell Biology, 17(1), 208-212. https://doi.org/10.1083/jcb.17.1.208
  • Sitjà-Bobadilla, A., & Alvarez-Pellitero, P. (2002). Experimental transmission of Cryptosporidium molnari (Apicomplexa: Coccidia) to Giltheadsea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax). Parasitology Research, 91, 209-214. https://doi.org/10.1007/s00436-003-0945-z
  • Sitjà-Bobadilla, A., Estensoro, I., & Pérez-Sánchez, J. (2016). Immunity to gastrointestinal microparasites of fish. Developmental and Comparative Immunology, 64, 187-201. https://doi.org/10.1016/j.dci.2016.01.014 Shrestha, B. (2022). Coccidiosis in fish. lifecycle of coccidiosis, development, pathogenesis and medications. GRIN Verlag, Munich, ISBN: 978-334-661-6524.
  • Steinhagen, D., Lukes, J. & Körting, W. (1990). Ultrasuctural observations on gamegonic stages of Goussia subepithelialis (Apicomplexa, Coccidia) from common carp Cyprinus carpio. Diseases of Aquatic Organisms, 9, 31-36. https://doi.org/10.3354/dao009031
  • Watson, M.L. (1958). Staining of tissue sections for electron microscopy with heavy metals: II. Application of solutions containing lead and barium. The Journal of Cell Biology, 4(6), 727-730. https://doi.org/10.1083/jcb.4.6.727
  • Yang, R., Dorrestein, G.M., & Ryan, U. (2016). Molecular characterisation of a disseminated Cryptosporidium infection in a Koi carp (Cyprinus carpio). Veterinary Parasitology, 226, 53-56. https://doi.org/10.1016/j.vetpar.2016.06.027
There are 24 citations in total.

Details

Primary Language English
Subjects Fish Pests and Diseases
Journal Section Research Articles
Authors

Tülay Akaylı 0000-0003-2375-2224

Çiğdem Ürkü 0000-0003-0381-9321

Project Number 2637
Early Pub Date August 23, 2023
Publication Date October 11, 2023
Submission Date July 19, 2023
Published in Issue Year 2023

Cite

APA Akaylı, T., & Ürkü, Ç. (2023). Coccidian infestation in cultured common pandora (Pagellus erythrinus). Aquatic Research, 6(4), 245-252. https://doi.org/10.3153/AR23023

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