The immobilization of
horseradish peroxidase in the form of cross-linked enzyme aggregates
(HRP–CLEAs) was optimized by three parameters full factorial experimental
design at two levels and the degradation of an azo dye was tested. The optimal
immobilization conditions were estimated as 0.06 mg enzyme/mL (0.96 U), 3 % (v/v,
Glutaraldehyde)) cross-linker ratio, and 6 mg /mL Bovine Serume Albumine amount,
respectively. The effects of variables were analysed by responce surface plots
and the enzyme concentration effect was insignificant for the tested levels. The
maximum CLEAs activity was 0.2188 U and the kinetic parameters (Km
and Vmax) were 0.0314 mM and 0.1044 mM/min while the calculated
values for soluble enzyme were 0.06 mM and 0.468 mM/min, respectively. Hydrogen
peroxide has inhibitory effect on enzyme aggregate activities at higher amounts
than 0.012 mM. Under optimal conditions, a textile azo dye, Reactive Blue 160
was completely oxidized within 90 h and higher temperatures also showed promising
results in dye degradation.
Cross-Linked Enzyme Aggregates (CLEAs) Horseradish Peroxidase Full Factorial Design Decolorization
Birincil Dil | İngilizce |
---|---|
Bölüm | Makaleler |
Yazarlar | |
Yayımlanma Tarihi | 31 Mart 2021 |
Gönderilme Tarihi | 18 Aralık 2018 |
Kabul Tarihi | 7 Mart 2021 |
Yayımlandığı Sayı | Yıl 2021 Cilt: 31 Sayı: 1 |