Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats

Arzu Gezer; Seda Yakut; Nurcan Kılıç Bargutalp; Berrin Tarakcı Gencer

Araştırma Makalesi

Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats

Yıl 2023, Cilt: 40 Sayı: 4, 740 - 747, 03.01.2024

Arzu Gezer Seda Yakut Nurcan Kılıç Bargutalp Berrin Tarakcı Gencer

Öz

It has become important over the last few years to reduce the detrimental effects of Acrylamide (ACR), which is the carcinogenic substance formed by the exposure of some foods to high temperatures. The present investigation evaluated the defensive effect of ferulic acid (FA) as opposed to toxicity in testis tissue triggered by ACR in male rats. ACR (100 ppm) was administered in drinking water alone or with FA for 30 days orally. This study revealed that treatment with FA could remarkably decrease MDA levels and significantly increase SOD levels in the testicular tissue of the ACR-treated rats. Intaking of FA as a supplement is also supported by increasing FSHR, LHR, and AR sex hormone receptors levels. FA also reduced the harmful effects leading to ACR by decreasing histopathological changes in the testicular tissue. This study demonstrated the probable protective mechanism of FA against testicular toxicity caused by ACR.

Anahtar Kelimeler

Acrylamide, Ferulic acid, testis, rat

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

1. Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J. Agric. Food Chem. 2002; 50(17): 4998-5006.
2. Tucker G, Featherstone S. Essentials of thermal processing: John Wiley & Sons; 2021.
3. Kaplan O, Kaya G, Ozcan C, Ince M, Yaman M. Acrylamide concentrations in grilled foodstuffs of Turkish kitchen by high performance liquid chromatography-mass spectrometry. Microchem. J. 2009; 93(2): 173-9.
4. Dearfield KL, Douglas GR, Ehling UH, Moore MM, Sega GA, Brusick DJ. Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Mutat Res-Fund Mol M. 1995; 330(1-2): 71-99.
5. Mojska H, Gielecińska I, Cendrowski A. Acrylamide content in cigarette mainstream smoke and estimation of exposure to acrylamide from tobacco smoke in Poland. AAEM 2016; 23(3).
6. Yang H-J, Lee S-H, Jin Y, Choi J-H, Han D-U, Chae C, et al. Toxicological effects of acrylamide on rat testicular gene expression profile. Reprod. Toxicol. 2005; 19(4): 527-34.
7. Erdemli Z, Erdemli ME, Turkoz Y, Gul M, Yigitcan B, Gozukara Bag H. The effects of acrylamide and Vitamin E administration during pregnancy on adult rats testis. Andrologia. 2019; 51(7): e13292.
8. Li L, Sun H-y, Liu W, Zhao H-y, Shao M-l. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FCT. 2017; 102:93-101.
9. Sengul E, Gelen V, Yildirim S, Cinar İ, Aksu EH. Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat. Environ. Toxicol. 2023; 38(4): 798-808.
10. Bostanci S, Koca I. Ferulıc Acıd As A Functıonal Food Compound And Its Importance. Full Text Proceedıngs Book.
11. Zduńska K, Dana A, Kolodziejczak A, Rotsztejn H. Antioxidant properties of ferulic acid and its possible application. Skin Pharmacol Physiol. 2018; 31(6): 332-6.
12. Ou S, Kwok KC. Ferulic acid: pharmaceutical functions, preparation and applications in foods. J. Sci. Food Agric. J SCI FOOD AGR. 2004; 84(11): 1261-9.
13. Brugiolo ASS, Gouveia ACC, de Souza Alves CC, e Silva FMdC, de Oliveira ÉE, Ferreira AP. Ferulic acid supresses Th2 immune response and prevents remodeling in ovalbumin-induced pulmonary allergy associated with inhibition of epithelial-derived cytokines. Pulm Pharmacol Ther. 2017; 45:202-9.
14. Rukkumani R, Aruna K, Suresh Varma P, Padmanabhan Menon V. Hepatoprotective role of ferulic acid: a dose-dependent study. J. Med. Food. 2004; 7(4): 456-61.
15. Bumrungpert A, Lilitchan S, Tuntipopipat S, Tirawanchai N, Komindr S. Ferulic acid supplementation improves lipid profiles, oxidative stress, and inflammatory status in hyperlipidemic subjects: A randomized, double-blind, placebo-controlled clinical trial. Nutrients. 2018; 10(6): 713.
16. Roy S, Metya SK, Rahaman N, Sannigrahi S, Ahmed F. Ferulic acid in the treatment of post‐diabetes testicular damage: Relevance to the down regulation of apoptosis correlates with antioxidant status via modulation of tgf‐β1, il‐1β and akt signalling. Cell Biochem. Funct. 2014; 32(1): 115-24.
17. Hasanein P, Fazeli F, Parviz M, Roghani M. Ferulic acid prevents lead‐induced testicular oxidative stress and suppressed spermatogenesis in rats. Andrologia. 2018; 50(1): e12798.
18. Takahashi M, Inoue K, Koyama N, Yoshida M, Irie K, Morikawa T, et al. Life stage-related differences in susceptibility to acrylamide-induced neural and testicular toxicity. Arch. Toxicol 2011; 85: 1109-20.
19. Hou T, Zhang L, Yang X. Ferulic acid, a natural polyphenol, protects against osteoporosis by activating SIRT1 and NF-κB in neonatal rats with glucocorticoid-induced osteoporosis. Biomed. Pharmacother 2019; 120: 109205.
20. Johnsen SG. Testicular biopsy score count–a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Horm Res Paediatr. 1970; 1(1): 2-25.
21. Gezer A, Karadag-Sari E. The role of amifostine in preventing radiotherapy induced testicular tissue damage in rats. Biotech. Histochem. 2022; 97(3): 215-21.
22. Gezer A, Sari EK. Investigation of apoptotic and autophagic effects of chronic roflumilast use on testicular tissue in rats by immunohistochemical and immunofluorescence methods. Iran. J. Basic Med. Sci.2023; 26(3): 276.
23. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem.1979; 95(2): 351-8.
24. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin. Chem 1988; 34(3): 497-500.
25. Stadler RH, Robert F, Riediker S, Varga N, Davidek T, Devaud S, et al. In-depth mechanistic study on the formation of acrylamide and other vinylogous compounds by the Maillard reaction. J. Agric. Food Chem. 2004; 52(17): 5550-8.
26. Radad K, El Amir Y, Al-Emam A, Al-Shraim M, Bin-Jaliah I, Krewenka C, et al. Minocycline protects against acrylamide-induced neurotoxicity and testicular damage in Sprague-Dawley rats. J. Toxicol. Pathol. 2020; 33(2): 87-95.
27. Alturki HA, Elsawy HA, Famurewa AC. Silymarin abrogates acrylamide‐induced oxidative stress‐mediated testicular toxicity via modulation of antioxidant mechanism, DNA damage, endocrine deficit and sperm quality in rats. Andrologia. 2022; 54(9): e14491.
28. Lebda M, Gad S, Gaafar H. Effects of lipoic acid on acrylamide induced testicular damage. Mater. Socio Med. 2014; 26(3): 208.
29. Dupré-Crochet S, Erard M, Nüβe O. ROS production in phagocytes: why, when, and where? J. Leukoc. Biol. 2013; 94(4): 657-70.
30. Chen W, Shen Y, Su H, Zheng X. Hispidin derived from Phellinus linteus affords protection against acrylamide-induced oxidative stress in Caco-2 cells. Chem. Biol. Interact. 2014; 219: 83-9.
31. Liu Z, Ren Z, Zhang J, Chuang C-C, Kandaswamy E, Zhou T, et al. Role of ROS and nutritional antioxidants in human diseases. Front. Physiol. 2018; 9: 477.
32. Ercal N, Gurer-Orhan H, Aykin-Burns N. Toxic metals and oxidative stress part I: mechanisms involved in metal-induced oxidative damage. Curr Top Med Chem. 2001; 1(6): 529-39.
33. Mancuso C, Santangelo R. Ferulic acid: pharmacological and toxicological aspects. FCT. 2014; 65: 185-95.
34. Kelainy EG, Ibrahim Laila IM, Ibrahim SR. The effect of ferulic acid against lead-induced oxidative stress and DNA damage in kidney and testes of rats. ESPR. 2019; 26: 31675-84.
35. Fiedan I, Ahmed E, Omar H. Acrylamide induced testicular toxicity in rats: protective effect of garlic oil. Biomark. 2015; 1(1): 5.
36. Kucukler S, Caglayan C, Darendelioğlu E, Kandemir FM. Morin attenuates acrylamide-induced testicular toxicity in rats by regulating the NF-κB, Bax/Bcl-2 and PI3K/Akt/mTOR signaling pathways. Life Sci. 2020; 261: 118301.
37. Saçık U, Çavdar Z, Ural C, Ersoy N, Özoğul C, Erbil G. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. Biotech. Histochem. 2023; 98(2): 77-85.
38. Kassab RB, Lokman MS, Daabo HM, Gaber DA, Habotta OA, Hafez MM, et al. Ferulic acid influences Nrf2 activation to restore testicular tissue from cadmium‐induced oxidative challenge, inflammation, and apoptosis in rats. J. Food Biochem. 2020; 44(12): e13505.
39. Gül M, Kayhan Kuştepe E, Erdemli ME, Altınöz E, Gözükara Bağ HG, Gül S, et al. Protective effects of crocin on acrylamide‐induced testis damage. Andrologia. 2021; 53(9): e14176.
40. Jin J-M, Yang W-X. Molecular regulation of hypothalamus pituitary gonads axis in males. Gene. 2014; 551(1): 15-25.
41. Yong E, Loy C, Sim K. Androgen receptor gene and male infertility. Hum. Reprod. Update. 2003; 9(1): 1-7.
42. Hu Jx, Li Yf, Li J, Pan C, He Z, Dong Hy, et al. Toxic effects of cypermethrin on the male reproductive system: with emphasis on the androgen receptor. J Appl Toxicol. 2013; 33(7): 576-85.
43. González CR, González B, Matzkin ME, Muñiz JA, Cadet JL, Garcia-Rill E, et al. Psychostimulant-induced testicular toxicity in mice: evidence of cocaine and caffeine effects on the local dopaminergic system. PLoS One. 2015; 10(11): e0142713.
44. Sun X, Sun H, Yu K, Wang Z, Liu Y, Liu K, et al. Aluminum chloride causes the dysfunction of testes through inhibiting the ATPase enzyme activities and gonadotropin receptor expression in rats. Biol. Trace Elem. Res. 2018; 183: 296-304.
45. Abd Al Haleem EN, Hasan WYS, Arafa, HMM. Therapeutic effects of thymoquinone or capsaicin on acrylamide-induced reproductive toxicity in rats mediated by their effect on oxidative stress, inflammation, and tight junction integrity. Drug Chem Toxicol. 2022; 45.5: 2328-2340.
46. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid. Med. Cell. Longev. 2014; 2014.
47. Das U, Manna K, Sinha M, Datta S, Das DK, Chakraborty A, et al. Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model. PloS one. 2014; 9(5): e97599.

Yıl 2023, Cilt: 40 Sayı: 4, 740 - 747, 03.01.2024

Arzu Gezer Seda Yakut Nurcan Kılıç Bargutalp Berrin Tarakcı Gencer

Öz

Proje Numarası

Kaynakça

1. Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J. Agric. Food Chem. 2002; 50(17): 4998-5006.
2. Tucker G, Featherstone S. Essentials of thermal processing: John Wiley & Sons; 2021.
3. Kaplan O, Kaya G, Ozcan C, Ince M, Yaman M. Acrylamide concentrations in grilled foodstuffs of Turkish kitchen by high performance liquid chromatography-mass spectrometry. Microchem. J. 2009; 93(2): 173-9.
4. Dearfield KL, Douglas GR, Ehling UH, Moore MM, Sega GA, Brusick DJ. Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Mutat Res-Fund Mol M. 1995; 330(1-2): 71-99.
5. Mojska H, Gielecińska I, Cendrowski A. Acrylamide content in cigarette mainstream smoke and estimation of exposure to acrylamide from tobacco smoke in Poland. AAEM 2016; 23(3).
6. Yang H-J, Lee S-H, Jin Y, Choi J-H, Han D-U, Chae C, et al. Toxicological effects of acrylamide on rat testicular gene expression profile. Reprod. Toxicol. 2005; 19(4): 527-34.
7. Erdemli Z, Erdemli ME, Turkoz Y, Gul M, Yigitcan B, Gozukara Bag H. The effects of acrylamide and Vitamin E administration during pregnancy on adult rats testis. Andrologia. 2019; 51(7): e13292.
8. Li L, Sun H-y, Liu W, Zhao H-y, Shao M-l. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FCT. 2017; 102:93-101.
9. Sengul E, Gelen V, Yildirim S, Cinar İ, Aksu EH. Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat. Environ. Toxicol. 2023; 38(4): 798-808.
10. Bostanci S, Koca I. Ferulıc Acıd As A Functıonal Food Compound And Its Importance. Full Text Proceedıngs Book.
11. Zduńska K, Dana A, Kolodziejczak A, Rotsztejn H. Antioxidant properties of ferulic acid and its possible application. Skin Pharmacol Physiol. 2018; 31(6): 332-6.
12. Ou S, Kwok KC. Ferulic acid: pharmaceutical functions, preparation and applications in foods. J. Sci. Food Agric. J SCI FOOD AGR. 2004; 84(11): 1261-9.
13. Brugiolo ASS, Gouveia ACC, de Souza Alves CC, e Silva FMdC, de Oliveira ÉE, Ferreira AP. Ferulic acid supresses Th2 immune response and prevents remodeling in ovalbumin-induced pulmonary allergy associated with inhibition of epithelial-derived cytokines. Pulm Pharmacol Ther. 2017; 45:202-9.
14. Rukkumani R, Aruna K, Suresh Varma P, Padmanabhan Menon V. Hepatoprotective role of ferulic acid: a dose-dependent study. J. Med. Food. 2004; 7(4): 456-61.
15. Bumrungpert A, Lilitchan S, Tuntipopipat S, Tirawanchai N, Komindr S. Ferulic acid supplementation improves lipid profiles, oxidative stress, and inflammatory status in hyperlipidemic subjects: A randomized, double-blind, placebo-controlled clinical trial. Nutrients. 2018; 10(6): 713.
16. Roy S, Metya SK, Rahaman N, Sannigrahi S, Ahmed F. Ferulic acid in the treatment of post‐diabetes testicular damage: Relevance to the down regulation of apoptosis correlates with antioxidant status via modulation of tgf‐β1, il‐1β and akt signalling. Cell Biochem. Funct. 2014; 32(1): 115-24.
17. Hasanein P, Fazeli F, Parviz M, Roghani M. Ferulic acid prevents lead‐induced testicular oxidative stress and suppressed spermatogenesis in rats. Andrologia. 2018; 50(1): e12798.
18. Takahashi M, Inoue K, Koyama N, Yoshida M, Irie K, Morikawa T, et al. Life stage-related differences in susceptibility to acrylamide-induced neural and testicular toxicity. Arch. Toxicol 2011; 85: 1109-20.
19. Hou T, Zhang L, Yang X. Ferulic acid, a natural polyphenol, protects against osteoporosis by activating SIRT1 and NF-κB in neonatal rats with glucocorticoid-induced osteoporosis. Biomed. Pharmacother 2019; 120: 109205.
20. Johnsen SG. Testicular biopsy score count–a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Horm Res Paediatr. 1970; 1(1): 2-25.
21. Gezer A, Karadag-Sari E. The role of amifostine in preventing radiotherapy induced testicular tissue damage in rats. Biotech. Histochem. 2022; 97(3): 215-21.
22. Gezer A, Sari EK. Investigation of apoptotic and autophagic effects of chronic roflumilast use on testicular tissue in rats by immunohistochemical and immunofluorescence methods. Iran. J. Basic Med. Sci.2023; 26(3): 276.
23. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem.1979; 95(2): 351-8.
24. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin. Chem 1988; 34(3): 497-500.
25. Stadler RH, Robert F, Riediker S, Varga N, Davidek T, Devaud S, et al. In-depth mechanistic study on the formation of acrylamide and other vinylogous compounds by the Maillard reaction. J. Agric. Food Chem. 2004; 52(17): 5550-8.
26. Radad K, El Amir Y, Al-Emam A, Al-Shraim M, Bin-Jaliah I, Krewenka C, et al. Minocycline protects against acrylamide-induced neurotoxicity and testicular damage in Sprague-Dawley rats. J. Toxicol. Pathol. 2020; 33(2): 87-95.
27. Alturki HA, Elsawy HA, Famurewa AC. Silymarin abrogates acrylamide‐induced oxidative stress‐mediated testicular toxicity via modulation of antioxidant mechanism, DNA damage, endocrine deficit and sperm quality in rats. Andrologia. 2022; 54(9): e14491.
28. Lebda M, Gad S, Gaafar H. Effects of lipoic acid on acrylamide induced testicular damage. Mater. Socio Med. 2014; 26(3): 208.
29. Dupré-Crochet S, Erard M, Nüβe O. ROS production in phagocytes: why, when, and where? J. Leukoc. Biol. 2013; 94(4): 657-70.
30. Chen W, Shen Y, Su H, Zheng X. Hispidin derived from Phellinus linteus affords protection against acrylamide-induced oxidative stress in Caco-2 cells. Chem. Biol. Interact. 2014; 219: 83-9.
31. Liu Z, Ren Z, Zhang J, Chuang C-C, Kandaswamy E, Zhou T, et al. Role of ROS and nutritional antioxidants in human diseases. Front. Physiol. 2018; 9: 477.
32. Ercal N, Gurer-Orhan H, Aykin-Burns N. Toxic metals and oxidative stress part I: mechanisms involved in metal-induced oxidative damage. Curr Top Med Chem. 2001; 1(6): 529-39.
33. Mancuso C, Santangelo R. Ferulic acid: pharmacological and toxicological aspects. FCT. 2014; 65: 185-95.
34. Kelainy EG, Ibrahim Laila IM, Ibrahim SR. The effect of ferulic acid against lead-induced oxidative stress and DNA damage in kidney and testes of rats. ESPR. 2019; 26: 31675-84.
35. Fiedan I, Ahmed E, Omar H. Acrylamide induced testicular toxicity in rats: protective effect of garlic oil. Biomark. 2015; 1(1): 5.
36. Kucukler S, Caglayan C, Darendelioğlu E, Kandemir FM. Morin attenuates acrylamide-induced testicular toxicity in rats by regulating the NF-κB, Bax/Bcl-2 and PI3K/Akt/mTOR signaling pathways. Life Sci. 2020; 261: 118301.
37. Saçık U, Çavdar Z, Ural C, Ersoy N, Özoğul C, Erbil G. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. Biotech. Histochem. 2023; 98(2): 77-85.
38. Kassab RB, Lokman MS, Daabo HM, Gaber DA, Habotta OA, Hafez MM, et al. Ferulic acid influences Nrf2 activation to restore testicular tissue from cadmium‐induced oxidative challenge, inflammation, and apoptosis in rats. J. Food Biochem. 2020; 44(12): e13505.
39. Gül M, Kayhan Kuştepe E, Erdemli ME, Altınöz E, Gözükara Bağ HG, Gül S, et al. Protective effects of crocin on acrylamide‐induced testis damage. Andrologia. 2021; 53(9): e14176.
40. Jin J-M, Yang W-X. Molecular regulation of hypothalamus pituitary gonads axis in males. Gene. 2014; 551(1): 15-25.
41. Yong E, Loy C, Sim K. Androgen receptor gene and male infertility. Hum. Reprod. Update. 2003; 9(1): 1-7.
42. Hu Jx, Li Yf, Li J, Pan C, He Z, Dong Hy, et al. Toxic effects of cypermethrin on the male reproductive system: with emphasis on the androgen receptor. J Appl Toxicol. 2013; 33(7): 576-85.
43. González CR, González B, Matzkin ME, Muñiz JA, Cadet JL, Garcia-Rill E, et al. Psychostimulant-induced testicular toxicity in mice: evidence of cocaine and caffeine effects on the local dopaminergic system. PLoS One. 2015; 10(11): e0142713.
44. Sun X, Sun H, Yu K, Wang Z, Liu Y, Liu K, et al. Aluminum chloride causes the dysfunction of testes through inhibiting the ATPase enzyme activities and gonadotropin receptor expression in rats. Biol. Trace Elem. Res. 2018; 183: 296-304.
45. Abd Al Haleem EN, Hasan WYS, Arafa, HMM. Therapeutic effects of thymoquinone or capsaicin on acrylamide-induced reproductive toxicity in rats mediated by their effect on oxidative stress, inflammation, and tight junction integrity. Drug Chem Toxicol. 2022; 45.5: 2328-2340.
46. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid. Med. Cell. Longev. 2014; 2014.
47. Das U, Manna K, Sinha M, Datta S, Das DK, Chakraborty A, et al. Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model. PloS one. 2014; 9(5): e97599.

Toplam 47 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Histoloji ve Embriyoloji
Bölüm	Research Article
Yazarlar	Arzu Gezer 0000-0002-1658-2098 Seda Yakut 0000-0003-1673-5661 Nurcan Kılıç Bargutalp 0000-0002-3584-5252 Berrin Tarakcı Gencer 0000-0002-5931-9919
Proje Numarası	-
Yayımlanma Tarihi	3 Ocak 2024
Gönderilme Tarihi	24 Temmuz 2023
Kabul Tarihi	10 Ekim 2023
Yayımlandığı Sayı	Yıl 2023 Cilt: 40 Sayı: 4

Kaynak Göster

APA	Gezer, A., Yakut, S., Kılıç Bargutalp, N., Tarakcı Gencer, B. (2024). Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats. Journal of Experimental and Clinical Medicine, 40(4), 740-747.
AMA	Gezer A, Yakut S, Kılıç Bargutalp N, Tarakcı Gencer B. Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats. J. Exp. Clin. Med. Ocak 2024;40(4):740-747.
Chicago	Gezer, Arzu, Seda Yakut, Nurcan Kılıç Bargutalp, ve Berrin Tarakcı Gencer. “Protective Role of Ferulic Acid on Acrylamide-Induced Testis Toxicity in Rats”. Journal of Experimental and Clinical Medicine 40, sy. 4 (Ocak 2024): 740-47.
EndNote	Gezer A, Yakut S, Kılıç Bargutalp N, Tarakcı Gencer B (01 Ocak 2024) Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats. Journal of Experimental and Clinical Medicine 40 4 740–747.
IEEE	A. Gezer, S. Yakut, N. Kılıç Bargutalp, ve B. Tarakcı Gencer, “Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats”, J. Exp. Clin. Med., c. 40, sy. 4, ss. 740–747, 2024.
ISNAD	Gezer, Arzu vd. “Protective Role of Ferulic Acid on Acrylamide-Induced Testis Toxicity in Rats”. Journal of Experimental and Clinical Medicine 40/4 (Ocak 2024), 740-747.
JAMA	Gezer A, Yakut S, Kılıç Bargutalp N, Tarakcı Gencer B. Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats. J. Exp. Clin. Med. 2024;40:740–747.
MLA	Gezer, Arzu vd. “Protective Role of Ferulic Acid on Acrylamide-Induced Testis Toxicity in Rats”. Journal of Experimental and Clinical Medicine, c. 40, sy. 4, 2024, ss. 740-7.
Vancouver	Gezer A, Yakut S, Kılıç Bargutalp N, Tarakcı Gencer B. Protective role of Ferulic acid on acrylamide-induced testis toxicity in rats. J. Exp. Clin. Med. 2024;40(4):740-7.

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