Öz
Type 2 Diabetes Mellitus (T2DM), a complex metabolic condition, is caused by a number of environmental and genetic factors, as well as their interactions. Many genes associated with T2DM have been discovered through genome-wide association studies. One of these genes is the SLC30A8 gene. T2DM and the SLC30A8 gene are linked to zinc, which is required for insulin secretion and storage. The aim of the research was to look into the relationship between T2DM disease and the rs13266634 polymorphism in the SLC30A8 gene. The research included 80 healthy people and 80 people with type 2 diabetes who were chosen at random from Şanlıurfa. A commercial kit was used to isolate DNA. Following isolation, the SLC30A8 gene region was amplified using PCR and cut with the HpaII enzyme. TT, CT, and CC alleles were discovered after cutting. We used data from 45 diagnosed T2DM cases and 52 confirmed healthy control subjects to detect alleles, and the gel imaging results were reliable and confident. The data analysis was evaluated using the SPSS method. The findings revealed a statistically significant relationship between the patient and control groups and the rs13266634 polymorphism in the SLC30A8 gene. Clarifying the genetic relationship between the rs13266634 polymorphism in the SLC30A8 gene and T2DM disease may aid in identifying genetic predispositions and developing risk profiles.
Anahtar Kelimeler
Diabetes mellitus Type 2 diabetes polymorphism SLC30A8 (rs13266634)
Destekleyen Kurum
Harran University Research Fund
Proje Numarası
22022
Teşekkür
For their significant contributions to our work, we would like to thank Assoc. Dr. to Özlem ÖZ, the Faculty Member to Çiğdem Cindioğlu and Fehime Dağ. This study was funded by Harran University Research Fund (Project No:22022)
Kaynakça
- 1. Nauck MA, Meier JJ. Glp-1 receptor agonists in type 1 diabetes: a MAG1C bullet?.The Lancet Diabetes Endocrinol. 2020; 8(4):262-264.
- 2. Danaei G, Finucane MM, Lu Y, Singh GM, Cowan MJ, Paciorek CJ, et al. Global burden of metabolic risk factors of chronic diseases collaborating group (blood glucose). national, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2·7 million participants. The Lancet. 2011; 378(9785):31-40. doi: 10.1016/S0140-6736(11)60679-X. Epub 2011 Jun 24. PMID: 21705069.
- 3. Kaku K. Pathophysiology of type 2 diabetes and ıts treatment policy. JMAJ. 2010; 53(1): 41-46.
- 4. Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE. 2014. “Global estimates of diabetes prevalence for 2013 and projections for 2035”. Diabetes Research and Clinical Practice. 2014;103 (2): 137-149.
- 5. Wild S, Roglic G, Green A, Sicree R, King H. 2004. “Global prevalence of diabetes estimates for the year 2000 and projections for 2030”. Diabetes Care. 2004; 27 (5):1047-1053.
- 6. Cheloni R, Gandolfi SA, Signorelli C, Odone A. Global prevalence of diabetic retinopathy: Protocol for a systematic review and meta-analysis. BMJ Open. 2019; 9(3):e022188.
- 7. Arıkoğlu H, Kaya DE. Tip 2 diyabetin moleküler genetik temeli; Son gelişmeler. Genel Tıp Derg. 2015; 25(4):147-159.
- 8. Faghih H, Khatami SR, Azarpira N, Foroughmand AM. SLC30A8 gene polymorphism (rs13266634 C/T) and type 2 diabetes mellitus in south Iranian population. Mol Biol Rep. 2014; 41(5): 2709–2715.
- 9. Khan IA, Jahan P, Hasan Q, Rao P. Validation of the association of TCF7L2 and SLC30A8 gene polymorphisms with post-transplant diabetes mellitus in Asian Indian population. Intractable Rare Dis. Res. 2015; 4(2): 87-92.
- 10. Khan IA, Poornima S, Jahan P, Rao P, Hasan Q. Type 2 diabetes mellitus and the association of candidate genes in Asian Indian population from Hyderabad, India. J. Clin. Diagn. Res. 2015; 9(11): Gc01-5.
- 11. Khan IA, Jahan P, Hasan Q, Rao P. Genetic confirmation of T2DM meta- analysis variants studied in gestational diabetes mellitus in an Indian population. Diabetes Metab. Syndrome. 2019; 13(1): 688–694.
- 12. Alharbi KK, Abudawood M, Khan IA. Amino-acid amendment of Arginine-325-Tryptophan in rs13266634 genetic polymorphism studies of the SLC30A8 gene with type 2 diabetes-mellitus patients featuring a positive family history in the Saudi population. Journal of King Saud University-Science. 2021; 33(1), 101258.
- 13. Fan M, Li W, Wang L, Gu S, Dong S, Chen M, et al. 2016. Association of SLC30A8 gene polymorphism with type 2 diabetes, evidence from 46 studies: a meta-analysis. Springer Nature. 2016; 53(2). 381-394.
- 14. Chimienti F, Devergnas S, Pattou F, Schuit F, Garcia-Cuenca R, Vandewalle B, et al. In vivo expression and functional characterization of the zinc transporter ZnT8 in glucose-induced insulin secretion. J Cell Sci. 2006; 119 (20): 4199–4206.
- 15. Nicolson TJ, Bellomo EA, Wijesekara N, Loder MK, Baldwin JM, Gyulkhandanyan AV, Insulin storage and glucose homeostasis in mice null for the granule zinc transporter ZnT8 and studies of the type 2 diabetes-associated variants. Diabetes. 2009; 58(9): 2070-2083.
- 16. Wu Y, Lı H, Loos RJ, YU Z, YE X, CHEN L, et al. Common variants in CDKAL1, CDKN2A/B, IGF2BP2, SLC30A8, and HHEX/IDE genes are associated with type 2 diabetes and impaired fasting glucose in a Chinese Han population. Diabetes, 2008; 57(10): 2834-2842.
- 17. Mtiraoui N, Turkı A, Nemr R, Echtay A, Izzıdı I, Al-Zaben GS, et al. Contribution of common variants of ENPP1, IGF2BP2, KCNJ11, MLXIPL, PPARγ, SLC30A8 and TCF7L2 to the risk of type 2 diabetes in Lebanese and Tunisian Arabs. Diabetes & metabolism, 2012; 38(5): 444-449.
- 18. Mashal S, Khanfar M, Al-Khalayfa S, Srour L, Mustafa L, Hakooz NM, et al. SLC30A8 gene polymorphism rs13266634 associated with increased risk for developing type 2 diabetes mellitus in Jordanian population. Gene.2021; 768: 14527.
Öz
Proje Numarası
22022
Kaynakça
- 1. Nauck MA, Meier JJ. Glp-1 receptor agonists in type 1 diabetes: a MAG1C bullet?.The Lancet Diabetes Endocrinol. 2020; 8(4):262-264.
- 2. Danaei G, Finucane MM, Lu Y, Singh GM, Cowan MJ, Paciorek CJ, et al. Global burden of metabolic risk factors of chronic diseases collaborating group (blood glucose). national, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2·7 million participants. The Lancet. 2011; 378(9785):31-40. doi: 10.1016/S0140-6736(11)60679-X. Epub 2011 Jun 24. PMID: 21705069.
- 3. Kaku K. Pathophysiology of type 2 diabetes and ıts treatment policy. JMAJ. 2010; 53(1): 41-46.
- 4. Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE. 2014. “Global estimates of diabetes prevalence for 2013 and projections for 2035”. Diabetes Research and Clinical Practice. 2014;103 (2): 137-149.
- 5. Wild S, Roglic G, Green A, Sicree R, King H. 2004. “Global prevalence of diabetes estimates for the year 2000 and projections for 2030”. Diabetes Care. 2004; 27 (5):1047-1053.
- 6. Cheloni R, Gandolfi SA, Signorelli C, Odone A. Global prevalence of diabetic retinopathy: Protocol for a systematic review and meta-analysis. BMJ Open. 2019; 9(3):e022188.
- 7. Arıkoğlu H, Kaya DE. Tip 2 diyabetin moleküler genetik temeli; Son gelişmeler. Genel Tıp Derg. 2015; 25(4):147-159.
- 8. Faghih H, Khatami SR, Azarpira N, Foroughmand AM. SLC30A8 gene polymorphism (rs13266634 C/T) and type 2 diabetes mellitus in south Iranian population. Mol Biol Rep. 2014; 41(5): 2709–2715.
- 9. Khan IA, Jahan P, Hasan Q, Rao P. Validation of the association of TCF7L2 and SLC30A8 gene polymorphisms with post-transplant diabetes mellitus in Asian Indian population. Intractable Rare Dis. Res. 2015; 4(2): 87-92.
- 10. Khan IA, Poornima S, Jahan P, Rao P, Hasan Q. Type 2 diabetes mellitus and the association of candidate genes in Asian Indian population from Hyderabad, India. J. Clin. Diagn. Res. 2015; 9(11): Gc01-5.
- 11. Khan IA, Jahan P, Hasan Q, Rao P. Genetic confirmation of T2DM meta- analysis variants studied in gestational diabetes mellitus in an Indian population. Diabetes Metab. Syndrome. 2019; 13(1): 688–694.
- 12. Alharbi KK, Abudawood M, Khan IA. Amino-acid amendment of Arginine-325-Tryptophan in rs13266634 genetic polymorphism studies of the SLC30A8 gene with type 2 diabetes-mellitus patients featuring a positive family history in the Saudi population. Journal of King Saud University-Science. 2021; 33(1), 101258.
- 13. Fan M, Li W, Wang L, Gu S, Dong S, Chen M, et al. 2016. Association of SLC30A8 gene polymorphism with type 2 diabetes, evidence from 46 studies: a meta-analysis. Springer Nature. 2016; 53(2). 381-394.
- 14. Chimienti F, Devergnas S, Pattou F, Schuit F, Garcia-Cuenca R, Vandewalle B, et al. In vivo expression and functional characterization of the zinc transporter ZnT8 in glucose-induced insulin secretion. J Cell Sci. 2006; 119 (20): 4199–4206.
- 15. Nicolson TJ, Bellomo EA, Wijesekara N, Loder MK, Baldwin JM, Gyulkhandanyan AV, Insulin storage and glucose homeostasis in mice null for the granule zinc transporter ZnT8 and studies of the type 2 diabetes-associated variants. Diabetes. 2009; 58(9): 2070-2083.
- 16. Wu Y, Lı H, Loos RJ, YU Z, YE X, CHEN L, et al. Common variants in CDKAL1, CDKN2A/B, IGF2BP2, SLC30A8, and HHEX/IDE genes are associated with type 2 diabetes and impaired fasting glucose in a Chinese Han population. Diabetes, 2008; 57(10): 2834-2842.
- 17. Mtiraoui N, Turkı A, Nemr R, Echtay A, Izzıdı I, Al-Zaben GS, et al. Contribution of common variants of ENPP1, IGF2BP2, KCNJ11, MLXIPL, PPARγ, SLC30A8 and TCF7L2 to the risk of type 2 diabetes in Lebanese and Tunisian Arabs. Diabetes & metabolism, 2012; 38(5): 444-449.
- 18. Mashal S, Khanfar M, Al-Khalayfa S, Srour L, Mustafa L, Hakooz NM, et al. SLC30A8 gene polymorphism rs13266634 associated with increased risk for developing type 2 diabetes mellitus in Jordanian population. Gene.2021; 768: 14527.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri (Diğer) |
| Bölüm | Research Articles |
| Yazarlar | |
| Proje Numarası | 22022 |
| Erken Görünüm Tarihi | 5 Ekim 2023 |
| Yayımlanma Tarihi | 25 Ekim 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 2 Sayı: 2 |
