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Sıçanlarda İndometazin ile İndüklenen Mide Ülseri Üzerine Badem ve Elma Yağının Koruyucu Etkileri

Yıl 2022, Cilt: 11 Sayı: 2, 196 - 201, 14.07.2022

Özlem Aydın Berktaş Fadime Atalay Dumlu

https://doi.org/10.53424/balikesirsbd.977959

Öz

Amaç: Günümüzde geleneksel ve alternatif tedavide bitkisel yağların kullanımı önem kazanmıştır. Özellikle doymamış yağ asit bileşimi fazla olan yağların tercih edilmesi birçok araştırmaya konu olmasına neden olmuştur.
Materyal ve Metot: Bu araştırmada da özellikle meyve ve kuruyemiş olarak kullanılan badem ve elma yağının koruyucu etkileri bir antienflamatuvar ilaç olan indometazin indüklemeli ülser modelinde belirlenmiştir. Deney grupları 0.5 ve 1 ml/kg dozda olacak şekilde badem ve elma yağı, pozitif kontrol famotidin grubu, negatif grup olarak da İND ve en son grup sağlıklı olacak şekilde belirlenmiştir. 24 saatlik açlık sonunda deney hayvanlarının mide dokularında biyokimyasal parametreler antiülser etkiyi ifade etmiştir.
Bulgular: Uygulanan İND ile artan LPO seviyeleri, KAT, GR ve MPx enzim aktiviteleri önemli oransa yağlar tarafından azaltılmıştır. Aynı zamanda uygulanan İND ile azalan GSH seviyelerini de badem ve elma yağı önemli oranda arttırmıştır.

Anahtar Kelimeler

elma yağı badem yağı indometazin ülser lipid peroksidasyonu elma yağı, badem yağı, indometazin, ülser, lipid peroksidasyonu

Kaynakça

  • Referans1. Guyton AC. Texbook of Medical Physiology, Physiology of Gastrointestinal Disorders. 7 th ed, Saunders Company, Philadelphia, 1986; 798-805.
  • Referans2. Crofford LJ, Lipsky PE, Brooksi P, Abramsoni SB, Simoni LS, Van De Puttei, L. Basic biology and clinical application of specific cyclooxygenase‐2 inhibitors. Arthritis & Rheumatology. 2000; 43 (1): 4-13.
  • Referans3. Atalay F, Odabasoglu F, Halici M, Cadirci E, Aydin-Berktas O, Halici Z, Cakir A. N-Acetyl Cysteine Has Both Gastro-Protective And Anti-Inflammatory Effects In Experimental Rat Models: Its Gastro-Protective Effect Is Related To Its In Vivo And In Vitro Antioxidant Properties. Journal of Cellular Biochemistry. 2016; 117:308-319.
  • Referans4. Uzkeser, H, Cadirci, E, Halici, Z, Odabasoglu, F, Polat, B, Yuksel, T.N, Ozaltin, S, Atalay, F, Anti-Inflammatory And Antinociceptive Effects Of Salbutamol On Acute And Chronic Models Of Inflammation In Rats: Involvement Of An Antioxidant Mechanism. Mediators Inflammation. 2012.
  • Referans5. Babujanarthanam, R, Kavitha, P, Mahadeva Rao, US, Pandian, MR, Quercitrin a bioflavonoid improves the antioxidant status in streptozotocin: induced diabetic rat tissues. Mol Cell Biochem. 2011; 358: 121–129.
  • Referans2. Chen, C.Y, Lapsley, K, Blumberg, J, A nutrition and health perspective on almonds. J Sci Food Agric, 2006, 86; 2245–2250.
  • Referans7. Atsu Barku VY, Nyarko HD, Dordunu P. Studies on the physicochemical characteristics, microbial load and storage stability of oil from Indian almond nut (Terminalia catappa l.). Food Sci Qualit Manage. 2012; 8: 9–17.
  • Referans8. Dong Q, Banaich MS, O’Brien PJ. Cytoprotection by almond skin extracts or catechins of hepatocyte cytotoxicity induced by hydroperoxide (oxidative stress model) versus glyoxal or methylglyoxal (carbonylation model). Chem Biol Interact. 2010; 185: 101-9. doi: 10.1016/j.cbi.2010.03.003.
  • Referans9. Aebi H. Catalase In Vitro, In: P. Lester (Ed.), Method. Enzymol. Academic Press. 1984; Pp. 121-126.
  • Referans10. Ohkawa H, Ohishi N, Yagi K. Assay For Lipid Peroxides In Animal Tissues By Thiobarbituric Acid Reaction. Anal Biochem. 1979; 95: 351-358.
  • Referans11. Sedlak JF, Lindsay RH. Estimation Of Total, Protein-Bound, And Nonprotein Sulfhydryl Groups In Tissue With Ellman's Reagent. Anal Biochem. 1968; 25: 192-205. 12. Referans12. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement Of Cutaneous Inflammation - Estimation of Neutrophil Content With an Enzyme Marker. J Invest Dermatol. 1982; 78: 206-209.
  • Referans13. Carlberg I, Mannervik B. Glutathione Reductase. Methods in Enzymology, 1985; 113: 484-490.
  • Referans14. Feldman M, Friedman LS, Sleisenger MH. Sleisenger and Fordtran’s Gastrointestinal and Liver Disease, Philadelphia: WB Saunders Co 6th ed. 2002; 604-615.
  • Referans15. Özgüven M, Kırıcı S. Farklı ekolojilerde Nane (Mentha) türlerinin verim ile uçucu yağ oran ve bileşenlerin araştırılması. Tr J Agriculture and Foresty. 1999; 23: 465-472.
  • Referans16. Dorman HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology. 2000; 88: 308-316.
  • Referans17. Baydar H. Yayla kekiği (Origanum minutiflorum O. Scwarz et. P. H. Davis)’nde farklı toplama zamanlarının uçucu yağ içeriği ve uçucu yağ bileşenleri üzerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi. 2005; 18(2): 175-178.
  • Referans18. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology. 2004; 94: 223- 253.
  • Referans19. Demir E, Yılmaz Ö. Streptozotosin ile Tip-1 diyabet oluşturulan sıçanlarda acı badem yağının serum ve eritrositlerdeki bazı biyokimyasal parametrelere etkisi. Marmara Pharmaceutical Journal. 2014; 18: 13-21.
  • Referans20. Dong Q, Banaich MS, O'Brien PJ. Cytoprotection by almond skin extracts or catechins of hepatocyte cytotoxicity induced by hydroperoxide (oxidative stress model) versus glyoxal or methylglyoxal (carbonylation model) Chem Biol Interact. 2010; 185: (2), 101-9.
  • Referans21. Sunil C, Ignacimuthu S, Kumarappan C. Hypolipidemic activity of Symplocos cochinchinensis S. Moore leaves in hyperlipidemic rats. J Nat Med, 2012; 66 (1): 32-8.
  • Referans22. Cheeseman K, Slater T. An introduction to free radical biochemistry. Brit Med Bul. 1993, 49; 481-493.
  • Referans23. Lee, S S.M, Role and regulation of cyclooxygenase-2 during inflammation. Am J Med, 1999; 106: 37-42.
  • Referans24. Somayyeh Abbasabad, Arab Mohammad Reza, Nikravesh Mahdi, Jalali Ali Reza, Fazel. “Evaluation of oxidative stress indices after exposure to malathion and protective effects of ascorbic acid in ovarian tissue of adult female rats”. Electronic Physician. 2018; 10 (5): 6789-6795.
  • Referans25. Yamasaki K, Kanbe T, Chijiwa T, Ishiyama H, Morita S. Gastric mucosal protection by OPC-12759, a novel antiulcer compound, in the rat. Eur J Pharm. 1987; 142: 23-29.
  • Referans26. Naito Y, Yoshikawa T, Matsuyama K, Nishimura S, Yagi M, Kondo M. Effects of free radical scavengers on indomethacin-induced aggravation of gastric ulcer in rats. Digest Dis Sci.1995; 40: 2019-2021.
  • Referans27. Hiraishi H, Terano A, Ota S, Mutoh H, Sugimoto T, Harada T, Razandi M, Ivey K. Protection of cultured rat gastric cells against oxidant-induced damage by exogenous glutathione. Gastroenterology.1994; 106: 1199-1207.
  • Referans28. Karaca M, Odabasoglu F, Kumtepe Y, Albayrak A, Cadirci E, Keles ON. Protective Effects Of Erythropoietin On Ischemia/Reperfusion Injury Of Rat Ovary. Eur J Obstet Gyn R B. 2009; 144: 157-162.
  • Referans29. Karakus B, Odabasoglu F, Cakir A, Halici Z, Bayir Y, Halici M, Aslan A, Suleyman H. The Effects Of Methanol Extract Of Lobaria Pulmonaria, A Lichen Species, On Indometacin‐Induced Gastric Mucosal Damage, Oxidative Stress And Neutrophil Infiltration. Phytotherap Res. 2009; 23: 635-639.
  • Referans30. Mizoguchi H, Ogawa Y, Kanatsu K, Tanaka A, Kato S, Takeuchi K. Protective Effect Of Rebamipide On Indomethacin‐Induced Intestinal Damage In Rats. J Gastro Hepato. 2001; 16: 1112-1119.
  • Referans31. Yadav SK, Adhikary B, Bandyopadhyay SK, Chattopadhyay S. Inhibition Of Tnf-Alpha, And Nf-Kappa B And Jnk Pathways Accounts For The Prophylactic Action Of The Natural Phenolic, Allylpyrocatechol Against Indomethacin Gastropathy. Bba-Gen Subjects. 2013; 1830:3776-3786.
  • Referans32. Fornai M, Natale G, Colucci R, Tuccori M, Carazzina G, Antonioli L, Baldi S, Lubrano V, Abramo A, Blandizzi C. Mechanisms of protection by pantoprazole against NSAID-induced gastric mucosal damage. N‐S Arch Pharmacol. 2005; 372: 79-87.

The protective Effects of Almond and Apple Oil on Indomethacin-Induced Stomach Ulcer in Rats

Yıl 2022, Cilt: 11 Sayı: 2, 196 - 201, 14.07.2022

Özlem Aydın Berktaş Fadime Atalay Dumlu

https://doi.org/10.53424/balikesirsbd.977959

Öz

Objective: Today, the use of vegetable oils in traditional and alternative therapy has gained importance. Especially the preference of oils with high unsaturated fatty acid composition has caused many researches.
Materials and Methods: In this study, the protective effects of almond and apple oil, especially used as fruits and nuts, were determined in the model of indomethacin-induced ulcer, an anti-inflammatory drug. The experimental groups were determined as 0.5 and 1 ml / kg of almond and apple oil, the positive control famotidine group, the negative group as IND and the last group as healthy.
Results: Biochemical parameters in the stomach tissues of experimental animals at the end of 24 hours of fasting expressed the antiulcer effect. Increased LPO levels, CAT, GR and MPx enzyme activities with the applied INDO were significantly reduced by the oils. At the same time, almond and apple oil significantly increased the GSH levels, which decreased with the application of IND.

Anahtar Kelimeler

almond oil apple oil indomethacin ulcer lipid peroxidation

Kaynakça

  • Referans1. Guyton AC. Texbook of Medical Physiology, Physiology of Gastrointestinal Disorders. 7 th ed, Saunders Company, Philadelphia, 1986; 798-805.
  • Referans2. Crofford LJ, Lipsky PE, Brooksi P, Abramsoni SB, Simoni LS, Van De Puttei, L. Basic biology and clinical application of specific cyclooxygenase‐2 inhibitors. Arthritis & Rheumatology. 2000; 43 (1): 4-13.
  • Referans3. Atalay F, Odabasoglu F, Halici M, Cadirci E, Aydin-Berktas O, Halici Z, Cakir A. N-Acetyl Cysteine Has Both Gastro-Protective And Anti-Inflammatory Effects In Experimental Rat Models: Its Gastro-Protective Effect Is Related To Its In Vivo And In Vitro Antioxidant Properties. Journal of Cellular Biochemistry. 2016; 117:308-319.
  • Referans4. Uzkeser, H, Cadirci, E, Halici, Z, Odabasoglu, F, Polat, B, Yuksel, T.N, Ozaltin, S, Atalay, F, Anti-Inflammatory And Antinociceptive Effects Of Salbutamol On Acute And Chronic Models Of Inflammation In Rats: Involvement Of An Antioxidant Mechanism. Mediators Inflammation. 2012.
  • Referans5. Babujanarthanam, R, Kavitha, P, Mahadeva Rao, US, Pandian, MR, Quercitrin a bioflavonoid improves the antioxidant status in streptozotocin: induced diabetic rat tissues. Mol Cell Biochem. 2011; 358: 121–129.
  • Referans2. Chen, C.Y, Lapsley, K, Blumberg, J, A nutrition and health perspective on almonds. J Sci Food Agric, 2006, 86; 2245–2250.
  • Referans7. Atsu Barku VY, Nyarko HD, Dordunu P. Studies on the physicochemical characteristics, microbial load and storage stability of oil from Indian almond nut (Terminalia catappa l.). Food Sci Qualit Manage. 2012; 8: 9–17.
  • Referans8. Dong Q, Banaich MS, O’Brien PJ. Cytoprotection by almond skin extracts or catechins of hepatocyte cytotoxicity induced by hydroperoxide (oxidative stress model) versus glyoxal or methylglyoxal (carbonylation model). Chem Biol Interact. 2010; 185: 101-9. doi: 10.1016/j.cbi.2010.03.003.
  • Referans9. Aebi H. Catalase In Vitro, In: P. Lester (Ed.), Method. Enzymol. Academic Press. 1984; Pp. 121-126.
  • Referans10. Ohkawa H, Ohishi N, Yagi K. Assay For Lipid Peroxides In Animal Tissues By Thiobarbituric Acid Reaction. Anal Biochem. 1979; 95: 351-358.
  • Referans11. Sedlak JF, Lindsay RH. Estimation Of Total, Protein-Bound, And Nonprotein Sulfhydryl Groups In Tissue With Ellman's Reagent. Anal Biochem. 1968; 25: 192-205. 12. Referans12. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement Of Cutaneous Inflammation - Estimation of Neutrophil Content With an Enzyme Marker. J Invest Dermatol. 1982; 78: 206-209.
  • Referans13. Carlberg I, Mannervik B. Glutathione Reductase. Methods in Enzymology, 1985; 113: 484-490.
  • Referans14. Feldman M, Friedman LS, Sleisenger MH. Sleisenger and Fordtran’s Gastrointestinal and Liver Disease, Philadelphia: WB Saunders Co 6th ed. 2002; 604-615.
  • Referans15. Özgüven M, Kırıcı S. Farklı ekolojilerde Nane (Mentha) türlerinin verim ile uçucu yağ oran ve bileşenlerin araştırılması. Tr J Agriculture and Foresty. 1999; 23: 465-472.
  • Referans16. Dorman HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology. 2000; 88: 308-316.
  • Referans17. Baydar H. Yayla kekiği (Origanum minutiflorum O. Scwarz et. P. H. Davis)’nde farklı toplama zamanlarının uçucu yağ içeriği ve uçucu yağ bileşenleri üzerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi. 2005; 18(2): 175-178.
  • Referans18. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology. 2004; 94: 223- 253.
  • Referans19. Demir E, Yılmaz Ö. Streptozotosin ile Tip-1 diyabet oluşturulan sıçanlarda acı badem yağının serum ve eritrositlerdeki bazı biyokimyasal parametrelere etkisi. Marmara Pharmaceutical Journal. 2014; 18: 13-21.
  • Referans20. Dong Q, Banaich MS, O'Brien PJ. Cytoprotection by almond skin extracts or catechins of hepatocyte cytotoxicity induced by hydroperoxide (oxidative stress model) versus glyoxal or methylglyoxal (carbonylation model) Chem Biol Interact. 2010; 185: (2), 101-9.
  • Referans21. Sunil C, Ignacimuthu S, Kumarappan C. Hypolipidemic activity of Symplocos cochinchinensis S. Moore leaves in hyperlipidemic rats. J Nat Med, 2012; 66 (1): 32-8.
  • Referans22. Cheeseman K, Slater T. An introduction to free radical biochemistry. Brit Med Bul. 1993, 49; 481-493.
  • Referans23. Lee, S S.M, Role and regulation of cyclooxygenase-2 during inflammation. Am J Med, 1999; 106: 37-42.
  • Referans24. Somayyeh Abbasabad, Arab Mohammad Reza, Nikravesh Mahdi, Jalali Ali Reza, Fazel. “Evaluation of oxidative stress indices after exposure to malathion and protective effects of ascorbic acid in ovarian tissue of adult female rats”. Electronic Physician. 2018; 10 (5): 6789-6795.
  • Referans25. Yamasaki K, Kanbe T, Chijiwa T, Ishiyama H, Morita S. Gastric mucosal protection by OPC-12759, a novel antiulcer compound, in the rat. Eur J Pharm. 1987; 142: 23-29.
  • Referans26. Naito Y, Yoshikawa T, Matsuyama K, Nishimura S, Yagi M, Kondo M. Effects of free radical scavengers on indomethacin-induced aggravation of gastric ulcer in rats. Digest Dis Sci.1995; 40: 2019-2021.
  • Referans27. Hiraishi H, Terano A, Ota S, Mutoh H, Sugimoto T, Harada T, Razandi M, Ivey K. Protection of cultured rat gastric cells against oxidant-induced damage by exogenous glutathione. Gastroenterology.1994; 106: 1199-1207.
  • Referans28. Karaca M, Odabasoglu F, Kumtepe Y, Albayrak A, Cadirci E, Keles ON. Protective Effects Of Erythropoietin On Ischemia/Reperfusion Injury Of Rat Ovary. Eur J Obstet Gyn R B. 2009; 144: 157-162.
  • Referans29. Karakus B, Odabasoglu F, Cakir A, Halici Z, Bayir Y, Halici M, Aslan A, Suleyman H. The Effects Of Methanol Extract Of Lobaria Pulmonaria, A Lichen Species, On Indometacin‐Induced Gastric Mucosal Damage, Oxidative Stress And Neutrophil Infiltration. Phytotherap Res. 2009; 23: 635-639.
  • Referans30. Mizoguchi H, Ogawa Y, Kanatsu K, Tanaka A, Kato S, Takeuchi K. Protective Effect Of Rebamipide On Indomethacin‐Induced Intestinal Damage In Rats. J Gastro Hepato. 2001; 16: 1112-1119.
  • Referans31. Yadav SK, Adhikary B, Bandyopadhyay SK, Chattopadhyay S. Inhibition Of Tnf-Alpha, And Nf-Kappa B And Jnk Pathways Accounts For The Prophylactic Action Of The Natural Phenolic, Allylpyrocatechol Against Indomethacin Gastropathy. Bba-Gen Subjects. 2013; 1830:3776-3786.
  • Referans32. Fornai M, Natale G, Colucci R, Tuccori M, Carazzina G, Antonioli L, Baldi S, Lubrano V, Abramo A, Blandizzi C. Mechanisms of protection by pantoprazole against NSAID-induced gastric mucosal damage. N‐S Arch Pharmacol. 2005; 372: 79-87.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Makaleler
Yazarlar

Özlem Aydın Berktaş 0000-0002-7235-4890

Fadime Atalay Dumlu 0000-0002-4504-4352

Yayımlanma Tarihi 14 Temmuz 2022
Gönderilme Tarihi 2 Ağustos 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 11 Sayı: 2

Kaynak Göster

APA Aydın Berktaş, Ö., & Atalay Dumlu, F. (2022). Sıçanlarda İndometazin ile İndüklenen Mide Ülseri Üzerine Badem ve Elma Yağının Koruyucu Etkileri. Balıkesir Sağlık Bilimleri Dergisi, 11(2), 196-201. https://doi.org/10.53424/balikesirsbd.977959
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