BİYOKÜTLE İÇİN MİKROALG VE SİYANOBAKTERİ’NİN BÜYÜK ÖLÇEKLİ ÜRETİMİ

Zeliha Demirel; Rüştü Tok; Işıl İlter; Saniye Akyıl; Ayşegül Erdoğan; Mehmet Koç; Figen Kaymak Ertekin; Meltem Conk Dalay

doi:10.3153/AR18008

Araştırma Makalesi

LARGE-SCALE PRODUCTION OF MICROALGAE AND CYANOBACTERIA FOR BIOMASS

Yıl 2018, , 64 - 76, 01.03.2018

Zeliha Demirel , Rüştü Tok Işıl İlter Saniye Akyıl Ayşegül Erdoğan , Mehmet Koç , Figen Kaymak Ertekin , Meltem Conk Dalay

https://doi.org/10.3153/AR18008

Öz

Microalgae and cyanobacteria have
been used a natural source of high-value compounds for pharmaceutical, food and
aquaculture industry for thousands of years, and the large-scale
cultivation of microalgae has existed for over half a century. More recently
novel species of microalgae and cyanobacteria have been identified and the
cultivation of algal biomass for various products is transitioning to
commercial-scale systems. Firstly, Arthrospira (Spirulina) platensis and Phaeodactylum tricornutum cultures grown in the sterile bottle (2L, 5L and 10L) in the laboratory
conditions were studied by means of specific growth rate and chlorophyll-a
analysis. Then, open and closed systems
were installed for large scale production of cyanobacteria and microalgae biomass industry for commercial application.
For the production of algae biomass, one open stir tank and two different types
of closed-cultured systems or photobioreactors were used (Polypropylene bags
and Plexiglas tubes) under environmental conditions. However, P. tricornutum has a good resistance to low
temperature and they can grow even under low light intensity conditions.
Comparing the performance of open pond systems, it was shown that the outdoor
systems with integrated temperature regulation resulted in a biomass production
similar to that for cultures grown in outdoor open ponds in regions with better
climatic conditions. Grown in our country increasing microalgae and cyanobacteria biomass productivity of can be achieved by designing advanced and developing
low cost technologies photobioreactors.

Anahtar Kelimeler

Arthrospira (Spirulina) platensis, Phaeodactylum tricornutum, Biomass, Photobioreactor

Kaynakça

Anonymus (2018a). Ege Üniversitesi Mikroalg Kültür Kolleksiyonu (2015). http://www.egemacc.com/cultures.php (accessed 10.01.2018)
Anonymus (2018b). Egert Doğal Ürünler Üretim Hayvancılık Gıda Yem İth. İhr. Paz. San. Tic Ltd. Şti. http://www.egert.com.tr/icerik/hakkimizda.aspx (accessed 5.01.2018)
Anonymus (2018c). T.C. Gıda Tarım ve Hayvancılık Bakanlığı, Su Ürünleri Yetiştiricilik Tesisleri (2018). https://www.tarim.gov.tr/BSGM/Belgeler/Icerikler/Su%20%C3%9Cr%C3%BCnleri%20Yeti%C5%9Ftiricili%C4%9Fi/Su-%C3%9Cr%C3%BCnleri-Tesisleri-09022018.pdf (accessed 21.02.2018)
Anonymus (2018d). Astronot yiyeceğinden yoğurt üretilecek. http://www.hurriyet.com.tr/astronot-yiyeceginden-yogurt-uretilecek-30292463 (accessed 4.01.2018)
Anonymus (2018e). Üniversitede "yosunlu ayran" ürettiler. http://www.haber7.com/yalova/1622866-universitede-yosunlu-ayran-urettiler (accessed 4.01.2018)
Anonymus (2018f). Mikroalg Gıda Tarım Sanayi Anonim Şirketi. http://mikroalg.com/portfoy/urunler/terradoc/ (accessed 4.01.2018) Anonymus (2018g). GPA Mühendislik. http://gpamuhendislik.com/home-page/satis/ (accessed 4.01.2018)
Anonymus (2018h). Marin Biyoteknoloji Ürünleri ve Gıda San. Tic. Ltd. Şti. http://www.marinbio.com.tr/urunler/aquaculture (accessed 4.01.2018)
Anonymus (2018ı). AKUATİK Su Ürünleri ve Kozmetik Ltd. Şti. https://www.akuatik.com.tr/urunlerimiz (accessed 4.01.2018)
Anonymus (2018i). Ege Biyoteknoloji A.Ş. http://www.egebiyoteknoloji.com/icerik.php?SayfaId=59345317 (accessed 4.01.2018)
Anonymus (2018j). Polidaş Polietilen Mam. Kim. San. Tic. Ltd. Şti. http://polidas.com.tr/icerik/ozel-tasarim-ve-imalat/8 (accessed 4.01.2018)
Anonymus (2018k). Fibrolpol Cam Takviyeli Plastik İth. İhr. San ve Tic. Ltd. Şti. http://www.fibropol.com/urun/alg-uretim-tanklari/ (accessed 4.01.2018)
Anonymus (2018l). Pikolab Mühendislik Biyoteknolojik Ürünler ve Lab. Hiz. San. Tic. Ltd. Şti. http://www.pikolab.com/index.php?route=product/product&product_id=143 (accessed 4.01.2018)
Anonymus (2018m). Nanosis Laboratuvar ve Test Sistemleri San. ve Tic. Ltd. Şti. http://www.nanosis.com.tr/haber/21/mikro-alg-uretim-tesisi.htm (accessed 4.01.2018)
Benavides, A.M.S., Torzillo, G., Kopecký, J., Masojídek, J. (2013). Productivity and biochemical composition of Phaeodactylum tricornutum (Bacillariophyceae) cultures grown outdoors in tubular photobioreactors and open ponds. Biomass and bioenergy, 54, 115-122.
Boussiba, S., Vonshak, A., Torzillo, G. (2004). Applied course on production and monitoring of microalgal growth, EbiltemYayınları, İzmir, 82.
Cisneros, M., Rito-Palomares, M. (2004). A simplified strategy for the release and primary recovery of c-phycocyanin produced by Spirulina maxima. Chem Biochem Eng Q, 18(4), 385-90.
Eriksen, N. T. (2016). Research Trends in the Dominating Microalgal Pigments, β-carotene, Astaxanthin, and Phycocyanin Used in Feed, in Foods, and in Health Applications. Journal of Nutrition and Food Sciences, 6(3), 1-6. Guillard, R.R., Ryther, J.H. (1962). Studies of marine planktonic diatoms: I. Cyclotella Nana Hustedt, and Detonula Confervacea (CLEVE) Gran. Canadian Journal of Microbiology, 8(2), 229-239.
Güler, F., Gülmez, B., (2008). Spirulina sp. ve Kullanım Alanları Üzerine Bir Araştırma, Erzincan Üniversitesi AquaClub Su Ürünleri Araştırma ve Geliştirme Bilim Kulübü Kemaliye, 5.Geleneksel Su Ürünleri Bilimsel ve Kültürel Platformu (Ulusal) 31 Mayıs-1 Haziran 2008, Erzincan, Kemaliye.
Harun, R., Singh, M., Forde, G.M., Danquah, M.K. (2010). Bioprocess engineering of microalgae to produce a variety of consumer products. Renewable and Sustainable Energy Reviews, 14(3), 1037-1047.
Kumar, M., Kulshreshtha, J., Singh, G.P. (2011). Growth and biopigment accumulation of cyanobacterium Spirulina platensis at different light intensities and temperature. Brazilian Journal of Microbiology, 42(3), 1128-1135.
Madkour, F.F., Kamil, A.E.W., Nasr, H.S. (2012). Production and nutritive value of Spirulina platensis in reduced cost media. The Egyptian Journal of Aquatic Research, 38(1), 51-57.
Papadaki, S., Kyriakopoulou, K., Tzovenis, I., Krokida, M. (2017). Environmental impact of phycocyanin recovery from Spirulina platensis cyanobacterium. Innovative Food Science & Emerging Technologies. 44,217-223.
Schoepp, N.G., Stewart, R.L., Sun, V., Quigley, A.J., Mendola, D., Mayfield, S.P., & Burkart, M.D. (2014). System and method for research-scale outdoor production of microalgae and cyanobacteria. Bioresource Technology, 166, 273-281.
Seely, G.R., Duncan, M.J., Vidaver, W.E. (1972). Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide. Marine Biology, 12(2), 184-188.
Tomaselli, L. (1997). Morphology, ultrastructure and taxonomy of Arthrospira (Spirulina) maxima and Arthrospira (Spirulina) platensis. Spirulina platensis (Arthrospira), Spirulina platensis (Arthrospira): physiology, cell-biology and biotechnology, 1-16.
Xia, S., Wang, K., Wan, L., Li, A., Hu, Q., Zhang, C. (2013). Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom Odontella aurita. Marine Drugs, 11(7), 2667-2681.
Yongmanitchai, W., Ward, O.P. (1991). Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Applied and Environmental Microbiology, 57(2), 419-425.