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Kiraz İçin Anaç Adayı Bazı Mahlep Genotiplerinin İn vitro Çoğaltımı

Year 2021, Volume: 31 Issue: 4, 847 - 857, 31.12.2021
https://doi.org/10.29133/yyutbd.892027

Abstract

Kiraz için anaç adayı olabilecek 3 adet mahlep genotipi ve SL 64 (St. Lucie 64) anacınınım in vitro şartlarında çoğaltılabilme olanakları araştırıldığı bu çalışmada eksplant olarak mahlep genotipleri ve SL 64 anacının sürgün uçları kullanılmıştır. Eksplantlar yüzey sterilizasyonu yapılarak başlangıç aşamasında 0.5 mg/l BAP (6-Benzilaminopurin) + 0.1 mg l-1 GA3 (Gibberellic acid) + 0.1 mg/l IBA (İndole-3-butyric acid) içeren MS (Murashige ve Skoog) besi ortamında kültüre alınmıştır. Çoğaltma ortamında farklı BAP (0, 0.5 ve 1 mg/l) dozlarının etkisini belirlemek için yapılan çalışmada sürgün sayısı 1.97-2.55 adet arasında değişmiştir. BAP dozunun artması ile sürgün sayısının arttığı belirlenirken, en fazla sürgün sayısı 1 mg/l BAP dozundan elde edilmiştir. Köklendirme aşamasında ise farklı IBA dozlarının (0, 0.5, 1 ve 2 mg/l) etkisi araştırılmıştır. Kallus oranı, köklenme oranı ve kök uzunluğu bakımından en iyi sonuçlar 2 mg/l IBA dozundan elde edilirken, köklü bitkinin uzunluğu ve yaprak sayısı bakımından ise en iyi sonuçlar 1 mg/l IBA dozundan elde edilmiştir.

Supporting Institution

Tarım ve Orman Bakanlığı Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü ile Ordu üniversitesi

Project Number

BBNB/10/10 numaralı proje ve TF 1506 numaralı proje

References

  • Aka-Kaçar, Y., Yılmaz, N., Yalçın-Mendi, Y., Küden, A., & Çetiner, S. (2001, September). Effects of different solidifying agents and different pH levels used in in vitro media on growth of some cherry (Prunus avium L.) rootstocks. I. Stone Seed Fruit Symposium, Yalova.
  • Aktürk, Z. (2009). In vitro micropropagation of cherry (Prunus avium L.). (PhD), Dicle University, Institute of Natural and Applied Sciences Diyarbakır, Turkey.
  • Aydın, E., Varol, İ., Demirsoy, L., Demirsoy, H., & Er, E. (2016). Production of Gisela 5, Gisela 6 and SL 64 rootstocks in different media. Yalova Atatürk Horticultural Research Institute Journal, 45(1), 487-491.
  • Bilginer, Ş., Ercişli, S., Gerçekcioğlu, R., Eşitgen, A., Güneş, M., Akbulut, M., Koç, A., & Çelik, T.Z. (2009). Cherry Rootstock Selectıon Breedıng In Central and Eastern Black Sea Regıon. Samsun, Turkey: The Scıentıfıc and Technologıcal Research Councıl of Turkey- Agriculture, Forestry and Veterinary Research Grant Committee- Project Final Report Number 106O031.
  • Borkowska, B., & Szczerba, J.(1991). Influence of different carbon sources on invertase activity and growth of sour cherry (Prunus cerasus L.) shoot cultures. J. of Exp. Botany 240(42), 911-915.
  • Bosnjakovic, D., Ognjanov, V., Barac, G., Ljubojevic., M, Pranjic, A., & Dugalic, K. (2013). Micropropagation of low-vigorous rootstock selections for sweet and sour cherry. Voc arstvo 47 (183/184), 121-128.
  • Canli, F. A., & Demir, F. (2014). In vitro multiplication and rooting of ‘F12-1’(Prunus avium L.) and ‘Maxma 14’(P. mahaleb L.× P. avium L.) rootstocks. Indian Journal of Horticulture, 71(2), 145-150.
  • Doriç, D., Ognjanov, V., Ljubojeviç, M., Baraç, G., Duliç, J., Pranjiç, A., & Dugaliç, K. (2014). Rapid propagation of sweet and sour cherry rootstocks. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 488-494.
  • Durkoviç, J. (2006). Rapid mikropropagation of mature wild cherry. Biologia Plantarum, 50(4), 733-736.
  • Ercişli, S., Eşitken, A., Orhan, E., & Özdemir, O. (2006). Rootstocks used for temperate fruit trees in Turkey: an overview. scientific works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture. Sodininkyste Ir Darzininkyste, 25(3): 27-33.
  • Fallahpour, M., Miri, S. M., & Bouzari, N. (2015). In vitro propagation of Gisela 5 rootstock as affected by mineral composition of media and plant growth regulators. Journal of Horticultural Research, 23(1), 57-64.
  • Güçlü, F,. Fatma, K., & Şan B. (2010). The in vitro micropropagation of some clonal cherry rootstocks. Journal of Natural and Applied Sciences, 14(2), 144-147. Hepaksoy, S. (2004). Researchs on micropropagation of some cherry rootstocks. Ege University, Journal of Agricultural Faculty, 41(3), 11-22.
  • Hosseinpour, B., Bouzari, N., Didar, Z., Masoumian, M., Ghaemmaghami, S. A., Ebrahimi, A., & Farvardin, A. (2015). High frequency in vitro propagation of MxM 60, a cherry rootstock: the effects of culture media and growth regulators. Iranian Journal of Genetics and Plant Breeding, 4(2), 28-36.
  • Kitin, P., Iliev I., Scaltsoyiannes A., Nellas C., Rubos A., & Funada R. A. (2005). Comparative histological study between normal and fasciated shoots of Prunus avium generated in vitro. Plant Cell, Tissue and Organ Culture, 82, 141-150.
  • Koç, A. (2009). A Research on cherry rootstock selectıon and their vegetative propagation potential ın Samsun province. (PhD), Ondokuz Mayıs Universıty, Instıtute of Natural and Applıed Sciences Samsun, Turkey.
  • Meraler S. A. (2010). Determination of mineral composition in different plant parts of mahaleb cherry (Prunus mahaleb L.). (Msc) Kilis 7 Aralık University Instıtute of Natural and Applıed Sciences Kilis, Turkey.
  • Morini, S., Sciutti, R., & Fortuna, P. (1992). In vitro growth response of Prunus cerasifera shoots as influenced by different light-dark cycles and sucrose concentrations. Plant Cell, Tissue and Organ Culture, 28(3), 245-248.
  • Özçağıran, R., Ünal, A., Özeker, E., & İsfendiyaroğlu, M. (2005). Temparate climate fruits, stone fruits vol. 1. Ege University, Journal of Agricultural Faculty 553, İzmir.
  • Özyurt, İ. K., & Akça, Y. (2017). Effects of water stress on biochemical changes of mahaleb (Prunus mahaleb L.) rootstocks. Journal of Agricultural Faculty of Gaziosmanpasa University, 34(3), 1-10.
  • Pakyürek, M. & Hepaksoy, S. (2019). In vitro propagation of Weiroot 158. Ejons Internatıonal Journal On Mathematıcs, Engıneerıng & Natural Scıences 108-118.
  • Shabani, Z., Moghadam, E. G., Abedi, B., & Tehranifar, A. (2015). The effect of plant growth regulators and their concentration in vitro on mass poropagation of Myrobalan 29C rootstock. Journal of Horticulture and Forestry, 7(3), 57-64.
  • Sharma, V., Thakur, M., & Kumar, A. (2017). An efficient method for in vitro propagation of Gisela 5 (Prunus cerasus x Prunus canescens)-clonal cherry rootstock. International Journal of Current Microbiology and Applied Sciences, 6(8), 2617-2624.
  • Skirvin, R.M. (1984). Stone Fruits. In P.V. Ammirato, D.A. Evans, W.R. Sharp & Y. Yamado (Eds), Handbook of Plant Cell Culture, Newyork, USA: Mc. Millan Publishing Company.
  • Skirvin, R.M., & Chu, M.C. (1979). In vitro propagation of “Forever Yours” rose. HortScience 15(4), 608-610.
  • Srinivasan, C., Isabel, M.G., & Scorza, R. (2005). Prunus spp. almond, apricot, cherry, nectarine, peach and plum. In R. E. Litz (Eds), Biotechnology of fruit and nut crops (pp 512-542). Wallingford, UK: CABI Publishing.
  • Sülüşoğlu, M. (2002). In vitro propagation of cherry rootstocks. (PhD) Ankara University, Instıtute of Natural and Applıed Sciences Ankara, Turkey.
  • Sülüşoğlu, M., & Çavuşoğlu, A.(2013). Micropropagation of cherry laurel Prunus laurocerasus L. Journal of Food, Agriculture and Environment, 11(1), 576-579.
  • Whiting M D, Lang G, & Opharth, D. (2005). Rootstocks and training system effect sweet cherry growth yield and fruit quality. HortScience, 40(3), 582-586.
  • Yıldırım, H. (2006). In vitro micropropagation of Hacıhaliloğlu (Prunus armenica L.) variety. (PhD), Dicle Universıty, Instıtute of Natural and Applıed Sciences Diyarbakır, Turkey

In Vitro Propagation of Some Mahaleb Genotypes as Candidate Rootstock for Sweet Cherries

Year 2021, Volume: 31 Issue: 4, 847 - 857, 31.12.2021
https://doi.org/10.29133/yyutbd.892027

Abstract

In this study, where carried out in 3 mahaleb genotypes and SL 64 (St. Lucie 64) rootstocks which can be rootstock candidate for cherry were investigated in order to determine the multiplication performance of in vitro conditions, shoot tips were used as explant of with mahaleb genotypes and SL 64 rootstocks. Surface sterilization was applied to the explants in the initial stage 0.5 mg l-1 BAP (6-Benzylaminopurine) +0.1 mg l-1 GA3 (Gibberellic acid) + 0.1 mg l-1IBA (Indole-3-butyric acid) was cultured in MS (Murashige and Skoog) medium. The study was carried out to determine the effect of different BAP (0, 0.5 and 1 mg l-1) doses during the shoot multiplication stage, the number of shoots was 1.97-2.55 in mahaleb genotypes. While the number of shoots increased with increasing BAP dose, the maximum number of shoots was obtained from 1 mg l-1 BAP dose. In the rooting stage, 0, 0.5, 1 and 2 IBA was added to ½ MS medium. The effect of different doses of IBA (0, 0.5, 1, 2 mg l-1) in rooting medium was investigated. While the best results in terms of callus ratio, rooting rate and root length were obtained from 2 mg l-1 IBA dose, the best results in terms of rooted plant length and number of leaves were obtained from 1 mg l-1 IBA dose.

Project Number

BBNB/10/10 numaralı proje ve TF 1506 numaralı proje

References

  • Aka-Kaçar, Y., Yılmaz, N., Yalçın-Mendi, Y., Küden, A., & Çetiner, S. (2001, September). Effects of different solidifying agents and different pH levels used in in vitro media on growth of some cherry (Prunus avium L.) rootstocks. I. Stone Seed Fruit Symposium, Yalova.
  • Aktürk, Z. (2009). In vitro micropropagation of cherry (Prunus avium L.). (PhD), Dicle University, Institute of Natural and Applied Sciences Diyarbakır, Turkey.
  • Aydın, E., Varol, İ., Demirsoy, L., Demirsoy, H., & Er, E. (2016). Production of Gisela 5, Gisela 6 and SL 64 rootstocks in different media. Yalova Atatürk Horticultural Research Institute Journal, 45(1), 487-491.
  • Bilginer, Ş., Ercişli, S., Gerçekcioğlu, R., Eşitgen, A., Güneş, M., Akbulut, M., Koç, A., & Çelik, T.Z. (2009). Cherry Rootstock Selectıon Breedıng In Central and Eastern Black Sea Regıon. Samsun, Turkey: The Scıentıfıc and Technologıcal Research Councıl of Turkey- Agriculture, Forestry and Veterinary Research Grant Committee- Project Final Report Number 106O031.
  • Borkowska, B., & Szczerba, J.(1991). Influence of different carbon sources on invertase activity and growth of sour cherry (Prunus cerasus L.) shoot cultures. J. of Exp. Botany 240(42), 911-915.
  • Bosnjakovic, D., Ognjanov, V., Barac, G., Ljubojevic., M, Pranjic, A., & Dugalic, K. (2013). Micropropagation of low-vigorous rootstock selections for sweet and sour cherry. Voc arstvo 47 (183/184), 121-128.
  • Canli, F. A., & Demir, F. (2014). In vitro multiplication and rooting of ‘F12-1’(Prunus avium L.) and ‘Maxma 14’(P. mahaleb L.× P. avium L.) rootstocks. Indian Journal of Horticulture, 71(2), 145-150.
  • Doriç, D., Ognjanov, V., Ljubojeviç, M., Baraç, G., Duliç, J., Pranjiç, A., & Dugaliç, K. (2014). Rapid propagation of sweet and sour cherry rootstocks. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 488-494.
  • Durkoviç, J. (2006). Rapid mikropropagation of mature wild cherry. Biologia Plantarum, 50(4), 733-736.
  • Ercişli, S., Eşitken, A., Orhan, E., & Özdemir, O. (2006). Rootstocks used for temperate fruit trees in Turkey: an overview. scientific works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture. Sodininkyste Ir Darzininkyste, 25(3): 27-33.
  • Fallahpour, M., Miri, S. M., & Bouzari, N. (2015). In vitro propagation of Gisela 5 rootstock as affected by mineral composition of media and plant growth regulators. Journal of Horticultural Research, 23(1), 57-64.
  • Güçlü, F,. Fatma, K., & Şan B. (2010). The in vitro micropropagation of some clonal cherry rootstocks. Journal of Natural and Applied Sciences, 14(2), 144-147. Hepaksoy, S. (2004). Researchs on micropropagation of some cherry rootstocks. Ege University, Journal of Agricultural Faculty, 41(3), 11-22.
  • Hosseinpour, B., Bouzari, N., Didar, Z., Masoumian, M., Ghaemmaghami, S. A., Ebrahimi, A., & Farvardin, A. (2015). High frequency in vitro propagation of MxM 60, a cherry rootstock: the effects of culture media and growth regulators. Iranian Journal of Genetics and Plant Breeding, 4(2), 28-36.
  • Kitin, P., Iliev I., Scaltsoyiannes A., Nellas C., Rubos A., & Funada R. A. (2005). Comparative histological study between normal and fasciated shoots of Prunus avium generated in vitro. Plant Cell, Tissue and Organ Culture, 82, 141-150.
  • Koç, A. (2009). A Research on cherry rootstock selectıon and their vegetative propagation potential ın Samsun province. (PhD), Ondokuz Mayıs Universıty, Instıtute of Natural and Applıed Sciences Samsun, Turkey.
  • Meraler S. A. (2010). Determination of mineral composition in different plant parts of mahaleb cherry (Prunus mahaleb L.). (Msc) Kilis 7 Aralık University Instıtute of Natural and Applıed Sciences Kilis, Turkey.
  • Morini, S., Sciutti, R., & Fortuna, P. (1992). In vitro growth response of Prunus cerasifera shoots as influenced by different light-dark cycles and sucrose concentrations. Plant Cell, Tissue and Organ Culture, 28(3), 245-248.
  • Özçağıran, R., Ünal, A., Özeker, E., & İsfendiyaroğlu, M. (2005). Temparate climate fruits, stone fruits vol. 1. Ege University, Journal of Agricultural Faculty 553, İzmir.
  • Özyurt, İ. K., & Akça, Y. (2017). Effects of water stress on biochemical changes of mahaleb (Prunus mahaleb L.) rootstocks. Journal of Agricultural Faculty of Gaziosmanpasa University, 34(3), 1-10.
  • Pakyürek, M. & Hepaksoy, S. (2019). In vitro propagation of Weiroot 158. Ejons Internatıonal Journal On Mathematıcs, Engıneerıng & Natural Scıences 108-118.
  • Shabani, Z., Moghadam, E. G., Abedi, B., & Tehranifar, A. (2015). The effect of plant growth regulators and their concentration in vitro on mass poropagation of Myrobalan 29C rootstock. Journal of Horticulture and Forestry, 7(3), 57-64.
  • Sharma, V., Thakur, M., & Kumar, A. (2017). An efficient method for in vitro propagation of Gisela 5 (Prunus cerasus x Prunus canescens)-clonal cherry rootstock. International Journal of Current Microbiology and Applied Sciences, 6(8), 2617-2624.
  • Skirvin, R.M. (1984). Stone Fruits. In P.V. Ammirato, D.A. Evans, W.R. Sharp & Y. Yamado (Eds), Handbook of Plant Cell Culture, Newyork, USA: Mc. Millan Publishing Company.
  • Skirvin, R.M., & Chu, M.C. (1979). In vitro propagation of “Forever Yours” rose. HortScience 15(4), 608-610.
  • Srinivasan, C., Isabel, M.G., & Scorza, R. (2005). Prunus spp. almond, apricot, cherry, nectarine, peach and plum. In R. E. Litz (Eds), Biotechnology of fruit and nut crops (pp 512-542). Wallingford, UK: CABI Publishing.
  • Sülüşoğlu, M. (2002). In vitro propagation of cherry rootstocks. (PhD) Ankara University, Instıtute of Natural and Applıed Sciences Ankara, Turkey.
  • Sülüşoğlu, M., & Çavuşoğlu, A.(2013). Micropropagation of cherry laurel Prunus laurocerasus L. Journal of Food, Agriculture and Environment, 11(1), 576-579.
  • Whiting M D, Lang G, & Opharth, D. (2005). Rootstocks and training system effect sweet cherry growth yield and fruit quality. HortScience, 40(3), 582-586.
  • Yıldırım, H. (2006). In vitro micropropagation of Hacıhaliloğlu (Prunus armenica L.) variety. (PhD), Dicle Universıty, Instıtute of Natural and Applıed Sciences Diyarbakır, Turkey
There are 29 citations in total.

Details

Primary Language English
Subjects Horticultural Production
Journal Section Articles
Authors

Erol Aydın 0000-0002-2710-4075

Tarık Yarılgaç 0000-0003-2097-7161

Project Number BBNB/10/10 numaralı proje ve TF 1506 numaralı proje
Publication Date December 31, 2021
Acceptance Date October 3, 2021
Published in Issue Year 2021 Volume: 31 Issue: 4

Cite

APA Aydın, E., & Yarılgaç, T. (2021). In Vitro Propagation of Some Mahaleb Genotypes as Candidate Rootstock for Sweet Cherries. Yuzuncu Yıl University Journal of Agricultural Sciences, 31(4), 847-857. https://doi.org/10.29133/yyutbd.892027
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