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Tıbbi Öneme Sahip Streptomyces Bakterilerinin Farklı Besiyerlerinde Çoğalma Oranlarının Belirlenmesi

Year 2021, Volume: 14 Issue: 3, 304 - 311, 30.12.2021
https://doi.org/10.52976/vansaglik.933104

Abstract

Aim: A total of 24 sediment samples were taken from the rivers flowing into Van Lake and their growth rates were examined in three different media. In our study, we aimed to investigate which Streptomyces (medically important) bacteria reproduce more in different medium.
Material-Method: In the study, a total of 24 sediment samples were taken from the rivers pouring into Van Lake. Then the moisture and pH of the samples were determined. Streptomyces bacteria were isolated from the samples taken and pure cultures of the bacteria were obtained. During the isolation process, three different media, Bennet's Agar, Medium 65 and SM3 Agar, were used.
Results: At the end of the isolation process, a total of 179 Streptomyces bacteria were isolated, 145 in Bennet's Agar medium, 8 in SM3 medium and 26 in Medium 65 medium, according to the counting process performed according to the morphological condition after the isolation process.
Conclusion: As a result, it was determined that Streptomyces bacteria isolated from sediment samples taken from fresh water grew mostly in Bennet's Agar medium among Bennet's Agar, SM3, and Medium 65 media. It is thought that Bennet's Agar medium can be used for the identification of new Streptomyces species producing secondary metabolites from aquatic environments.

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Project Number

2017-FDK-5054

References

  • Amin, A., Ahmed, I., Khalid, N., Osman, G., Khan, I.U., Xiao, M., Li, W.J. (2016). Streptomyces caldifontis sp. nov., isolated from a hot water spring of Tatta Pani, Kotli, Pakistan. Antonie van Leeuwenhoek 110,77-86
  • Araujo, F.V., Rosa, CA., Freitas, L.F.D., Lachance, M.A., Vaughan-Martini, A., Mendonca Hagler, L.C., Hagler, A.N. (2012). “Kazachstania bromeliacearum sp. nov., a yeast species from water tanks of bromeliads”. International J Syst. Evolution. Microbiol. 62(4),1002-1006.
  • Atalan, E., 1995. Identification of Streptomyces isolated from environmental soil samples using rapid enzyme data. Journal of Environmental Science and Health Part A 30, 1133-1143.
  • Awla, F.M., Özdemir, K., Ertaş, M. (2017). Determination of Extracellular Hydrolytic Enzyme Production Capacity and 16s rDNA Analysis of Streptomyces Bacteria Isolated From Some Soil Samples Collected In Erbil Iraq. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi/ Journal of the Institute of Natural & Applied Sciences 22, 132-138.
  • Chellapandi, P., Jani, H.M. 2008. Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation. Braz J Microbiol 39, 122-127.
  • Cheng, C., Li, Y.Q., Asem, M.D., Lu, C.Y., Shi, X.H., X.C., Li, W.J. (2016). Streptomyces xinjiangensis sp. nov., an actinomycetes isolated from Lop Nur region. Arch. Microbiol 198, 785-791.
  • Demain, A.L. (2014). Importance of microbial natural products and the need to revitalize their discovery. J. Ind. Microbiol. Biotechnol 41, 185-201
  • Ertaş, M., Özdemir, K., Atalan, E. (2013). Isolation and characterization of Micromonospora bacteria from various soil samples obtained around Lake Van. African Journal of Biotechnology 12, 3283-3287.
  • Jia, F.Y., Liu, C.X., Zhao, J.W., Zhang, Y.J., Li, L.J., Zhou, S.Y., Shen, Y., Wang, X.J., Xiang, W.S. (2015). Streptomyces vulcanius sp. nov. a novel actinomycete isolated from volcani sediment. Antonie Van Leeuwenhoek 107, 15-21.
  • Jones, K.L. (1949). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelyum is fluctuating characteristic. Journal of Bacteriology 57, 141-146.
  • Lewin, G.R., Carlos, C., Chevrette, M.G., Horn, H.A., McDonald, B.R., Stankey, R.J., Fox, B.J., Currie, C.R. (2015). “Evolution and Ecology of Actinobacteria and Their Bioenerg Applications”. Annu.Rev.Microbiol 70, 235-54.
  • Mokraneet, S., Bouras, N., Sabaou, N., Mathieu, F. (2013). Actinomycetes from saline and non-saline soils of Saharan palm groves: Taxonomy, ecology and antagonistic properties. African Journal of Microbiology Research 7, 2167-2178.
  • Özdemir K., Lens orientalis (Boiss.) Hand & Mazz ve Cicer anatolicium Alef. Rizosferden Streptomyces Türlerinin İzolasyonu, Teşhisi ve Karekterizasyonu. (Doktora tezi). YYÜ. Fen Bilimleri Enstitüsü, 2008.
  • Özok, Ö., Özdemir, K. (2019). Sakarya ili ormanlık alan topraklarından Streptomyces türlerinin izolasyonu, karakterizasyonu ve bazı ekstraselüler enzimlerin üretiminin belirlenmesi. Toprak Bilimi ve Bitki Besleme Dergisi 7, 64-71.
  • Sarigüllü Önalan, F.E. (2020). Streptomyces Suşlarında Farklı Genetik Manipulasyonların Ksilinaz Enzim Üretimi Üzerine Etkisi. Anadolu Çev. ve Hay. Dergisi 5, 224-230.
  • Semêdo, L., Gomes, R.C., Linhares, A.A., Duarte, G.F., Nascimento, R.P., Rosado, A.S., Margis-Pinheiro, M., Margis, R., Silva, K., Alviano, C.S., Manfio, G.P., Soares, R., Linhares, L.F.,
  • Coelho, R. (2004). Streptomyces drozdowiczii sp. nov., a novel cellulolytic streptomycete from soil in Brazil. Int. J. Syst. Evol. Microbiol 54, 1323-1328.
  • Sharma, KT., Mawlankar, R., Sonalkar, VV., Shinde, VK., Zhan, J., Li, WJ., Rele, MV., Dastager, SG., Kumar LS. (2016). “Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India”. Antonie van Leeuwenhoek 109: 225–235.
  • Tan, G.Y.A., Ward, A.C., Goodfellow, M. (2006). Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Systematic and Applied Microbiology, 29, 557-569.
  • Ünal, D.H, Özdemir, K. (2019). Sapanca Gölü (Sakarya) Havzası Toprak ve Sedimentlerinden Streptomyces Cinsi Bakterilerin İzolasyonu, Karakterizasyonu ve Teşhisi. BEÜ Fen Bilimleri Dergisi 8, 826-834.
  • Veyisoglu, A., Sahin, N. (2014). 1Streptomyces hoynatensis sp. nov., isolated from deep marine sediment. Int J Syst Evol Microbiol 64, 819-826.
  • Williams, S.T., Goodfellow, M., Alderson, G. (1989). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Berys’s Manual of Systematıc Bacteriology, Volume 4, Edited by S.T. Williams, M.E. Sharpe, J.G. Holt. Williams and Wilkins. Baltimore, 2452-2492.

Determination of the Growth Rate of Medicinally Important Streptomyces Bacteria in Different Media

Year 2021, Volume: 14 Issue: 3, 304 - 311, 30.12.2021
https://doi.org/10.52976/vansaglik.933104

Abstract

Objective: A total of 24 sediment samples were taken from the rivers flowing into Van Lake and their growth rates were examined in three different media. In our study, we aimed to investigate which Streptomyces (medi-cally important) bacteria reproduce more in different medium.
Material and Method: In the study, a total of 24 sediment samples were taken from the rivers pouring into Van Lake. Then the moisture and pH of the samples were determined. Streptomyces bacteria were isolated from the samples taken and pure cultures of the bacteria were obtained. During the isolation process, three different me-dia, Modified Bennett's Agar, Medium 65 and SM3 Agar, were used.
Results: At the end of the isolation process, a total of 179 Streptomyces bacteria were isolated, 145 in Modified Bennett's Agar medium, 8 in SM3 medium and 26 in Medium 65 medium, according to the counting process performed according to the morphological condition after the isolation process.
Conclusion: As a result, it was determined that Streptomyces bacteria isolated from sediment samples taken from fresh water grew mostly in Modified Bennett's Agar medium among Modified Bennett's Agar, SM3 and Medium 65 media. It is thought that Modified Bennett's Agar medium can be used for the identification of new Streptomyces species producing secondary metabolites from aquatic environments

Project Number

2017-FDK-5054

References

  • Amin, A., Ahmed, I., Khalid, N., Osman, G., Khan, I.U., Xiao, M., Li, W.J. (2016). Streptomyces caldifontis sp. nov., isolated from a hot water spring of Tatta Pani, Kotli, Pakistan. Antonie van Leeuwenhoek 110,77-86
  • Araujo, F.V., Rosa, CA., Freitas, L.F.D., Lachance, M.A., Vaughan-Martini, A., Mendonca Hagler, L.C., Hagler, A.N. (2012). “Kazachstania bromeliacearum sp. nov., a yeast species from water tanks of bromeliads”. International J Syst. Evolution. Microbiol. 62(4),1002-1006.
  • Atalan, E., 1995. Identification of Streptomyces isolated from environmental soil samples using rapid enzyme data. Journal of Environmental Science and Health Part A 30, 1133-1143.
  • Awla, F.M., Özdemir, K., Ertaş, M. (2017). Determination of Extracellular Hydrolytic Enzyme Production Capacity and 16s rDNA Analysis of Streptomyces Bacteria Isolated From Some Soil Samples Collected In Erbil Iraq. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi/ Journal of the Institute of Natural & Applied Sciences 22, 132-138.
  • Chellapandi, P., Jani, H.M. 2008. Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation. Braz J Microbiol 39, 122-127.
  • Cheng, C., Li, Y.Q., Asem, M.D., Lu, C.Y., Shi, X.H., X.C., Li, W.J. (2016). Streptomyces xinjiangensis sp. nov., an actinomycetes isolated from Lop Nur region. Arch. Microbiol 198, 785-791.
  • Demain, A.L. (2014). Importance of microbial natural products and the need to revitalize their discovery. J. Ind. Microbiol. Biotechnol 41, 185-201
  • Ertaş, M., Özdemir, K., Atalan, E. (2013). Isolation and characterization of Micromonospora bacteria from various soil samples obtained around Lake Van. African Journal of Biotechnology 12, 3283-3287.
  • Jia, F.Y., Liu, C.X., Zhao, J.W., Zhang, Y.J., Li, L.J., Zhou, S.Y., Shen, Y., Wang, X.J., Xiang, W.S. (2015). Streptomyces vulcanius sp. nov. a novel actinomycete isolated from volcani sediment. Antonie Van Leeuwenhoek 107, 15-21.
  • Jones, K.L. (1949). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelyum is fluctuating characteristic. Journal of Bacteriology 57, 141-146.
  • Lewin, G.R., Carlos, C., Chevrette, M.G., Horn, H.A., McDonald, B.R., Stankey, R.J., Fox, B.J., Currie, C.R. (2015). “Evolution and Ecology of Actinobacteria and Their Bioenerg Applications”. Annu.Rev.Microbiol 70, 235-54.
  • Mokraneet, S., Bouras, N., Sabaou, N., Mathieu, F. (2013). Actinomycetes from saline and non-saline soils of Saharan palm groves: Taxonomy, ecology and antagonistic properties. African Journal of Microbiology Research 7, 2167-2178.
  • Özdemir K., Lens orientalis (Boiss.) Hand & Mazz ve Cicer anatolicium Alef. Rizosferden Streptomyces Türlerinin İzolasyonu, Teşhisi ve Karekterizasyonu. (Doktora tezi). YYÜ. Fen Bilimleri Enstitüsü, 2008.
  • Özok, Ö., Özdemir, K. (2019). Sakarya ili ormanlık alan topraklarından Streptomyces türlerinin izolasyonu, karakterizasyonu ve bazı ekstraselüler enzimlerin üretiminin belirlenmesi. Toprak Bilimi ve Bitki Besleme Dergisi 7, 64-71.
  • Sarigüllü Önalan, F.E. (2020). Streptomyces Suşlarında Farklı Genetik Manipulasyonların Ksilinaz Enzim Üretimi Üzerine Etkisi. Anadolu Çev. ve Hay. Dergisi 5, 224-230.
  • Semêdo, L., Gomes, R.C., Linhares, A.A., Duarte, G.F., Nascimento, R.P., Rosado, A.S., Margis-Pinheiro, M., Margis, R., Silva, K., Alviano, C.S., Manfio, G.P., Soares, R., Linhares, L.F.,
  • Coelho, R. (2004). Streptomyces drozdowiczii sp. nov., a novel cellulolytic streptomycete from soil in Brazil. Int. J. Syst. Evol. Microbiol 54, 1323-1328.
  • Sharma, KT., Mawlankar, R., Sonalkar, VV., Shinde, VK., Zhan, J., Li, WJ., Rele, MV., Dastager, SG., Kumar LS. (2016). “Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India”. Antonie van Leeuwenhoek 109: 225–235.
  • Tan, G.Y.A., Ward, A.C., Goodfellow, M. (2006). Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Systematic and Applied Microbiology, 29, 557-569.
  • Ünal, D.H, Özdemir, K. (2019). Sapanca Gölü (Sakarya) Havzası Toprak ve Sedimentlerinden Streptomyces Cinsi Bakterilerin İzolasyonu, Karakterizasyonu ve Teşhisi. BEÜ Fen Bilimleri Dergisi 8, 826-834.
  • Veyisoglu, A., Sahin, N. (2014). 1Streptomyces hoynatensis sp. nov., isolated from deep marine sediment. Int J Syst Evol Microbiol 64, 819-826.
  • Williams, S.T., Goodfellow, M., Alderson, G. (1989). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Berys’s Manual of Systematıc Bacteriology, Volume 4, Edited by S.T. Williams, M.E. Sharpe, J.G. Holt. Williams and Wilkins. Baltimore, 2452-2492.
There are 22 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Original Research Articles
Authors

Hamdullah Seçkin 0000-0003-3884-4121

Kerem Özdemir 0000-0002-2188-0612

Project Number 2017-FDK-5054
Publication Date December 30, 2021
Submission Date May 5, 2021
Published in Issue Year 2021 Volume: 14 Issue: 3

Cite

APA Seçkin, H., & Özdemir, K. (2021). Determination of the Growth Rate of Medicinally Important Streptomyces Bacteria in Different Media. Van Sağlık Bilimleri Dergisi, 14(3), 304-311. https://doi.org/10.52976/vansaglik.933104

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