Research Article
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Year 2021, Volume: 5 Issue: 2, 408 - 417, 31.10.2021

Abstract

References

  • Aazami, M. A., Torabi, M., & Jalili, E. (2010). In vitro response of promising tomato genotypes for tolerance to osmotic stress. African Journal of Biotechnology, 9(26), 4014-4017.
  • Ahmad, M. A., Javed, R., Adeel, M., Rizwan, M., & Yang, Y. (2020). PEG 6000-Stimulated Drought Stress Improves the Attributes of In Vitro Growth, Steviol Glycosides Production, and Antioxidant Activities in Stevia rebaudiana Bertoni. Plants, 9(11), 1552.
  • Algrech, C. (2001). The saffron of Quercy. Rev. Quercy Rech.,97, 20–27.
  • Aslezaeem, F., Niknam, V., Ebrahimzadeh, H., & Sharifi, G. (2018). Comparative study of drought stress and salicylic acid effects on different accessions of saffron (Crocus Sativus L.), 554-569.
  • Biswas, J., Chowdhury, B., Bhattacharya, A., & Mandal, A. B. (2002). In vitro screening for increased drought tolerance in rice. In Vitro Cellular & Developmental Biology-Plant, 38(5), 525-530.
  • Boris, P. M., Dubravka, Š., Ružica, Ž. P., Bojana, B., & Vladislava, G. Antioxidative Response Of Poplar Tissue Culture Exposed To Peg. 22nd International Symposium on Analytical and Environmental Problems,267-269.
  • Bouiamrine, E. H., & Diouri, M. (2012). Response of durum wheat (Triticum durum Desf.) callus culture to osmosis-induced drought stress caused by polyethylene glycol (PEG). Annals of Biological Research, 3(9), 4555-4563.
  • Dami, I., & Hughes, H. (1995). Leaf anatomy and water loss of in vitro PEG-treated ‘Valiant’grape. Plant cell, tissue and organ culture, 42(2), 179-184.
  • Dami, I., & Hughes, H. G. (1997). Effects of PEG-induced water stress on in vitro hardening of ‘Valiant’grape. Plant Cell, Tissue and Organ Culture, 47(2), 97-101.
  • Duncan, R. R., Waskom, R. M., & Nabors, M. W. (1995). In vitro screening and field evaluation of tissue-culture-regenerated sorghum (Sorghum bicolor (L.) Moench) for soil stress tolerance. In The Methodology of Plant Genetic Manipulation: Criteria for Decision Making (pp. 373-380). Springer, Dordrecht.
  • Dwyer, A., Hawrelak, J., Whitten, D.(2011). Herbal Medicines, other than St. John’s Wort, in the treatment of Depression: A systematic Review. Sci. Rev. Altern. Med. 16, 40–49.
  • Fallon, K. M., & Phillips, R. (1989). Responses to water stress in adapted and unadapted carrot cell suspension cultures. Journal of Experimental Botany, 40(6), 681-687.
  • Farooq M, Basra S. M. A., Wahid A., Cheema Z. A., Cheema M. A., & Khaliq A. (2008). Physiological role of exogenously applied glycinebetaine in improve drought tolerance of fine grain aromatic rice (Oryza sativa L.). J. Agron. Crop Sci, 194, 325-333.
  • Gao, H., Xu, D., Zhang, H., Cheng, X., & Yang, Q. (2020). Effects of culture medium composition and PEG on hyperhydricity in Dendrobium officinale. In Vitro Cellular & Developmental Biology-Plant, 56(2), 143-149.
  • Ghassemi-Golezani, K., Farhadi, N., & Nikpour-Rashidabad, N. (2018). Responses of in vitro-cultured Allium hirtifolium to exogenous sodium nitroprusside under PEG-imposed drought stress. Plant Cell, Tissue and Organ Culture (PCTOC), 133(2), 237-248.
  • Guclu, G., Kelebek, H., & Selli, S. (2020). Saffron (Crocus sativus L.): Its Aroma and Key Odorants. In Saffron (pp. 69-82). Academic Press.
  • Hajihashemi, S., & Ehsanpour, A. A. (2014). Antioxidant response of Stevia rebaudiana B. to polyethylene glycol and paclobutrazol treatments under in vitro culture. Applied biochemistry and biotechnology, 172(8), 4038-4052.
  • Hill, T. (2004). The contemporary encyclopedia of herbs & spices: seasonings for the global kitchen. J. Wiley.
  • Hongbo, S., Zongsuo, L., Mingan, S., & Bochu, W. (2005). Impacts of PEG-6000 pretreatment for barley (Hordeum vulgare L.) seeds on the effect of their mature embryo in vitro culture and primary investigation on its physiological mechanism. Colloids and Surfaces B: Biointerfaces, 41(2-3), 73-77.
  • Khazdair, M. R., Anaeigoudari, A., Hashemzehi, M., & Mohebbati, R. (2019). Neuroprotective potency of some spice herbs, a literature review. Journal of traditional and complementary medicine, 9(2), 98-105.
  • Mahmood, I., Razzaq, A., Ashraf, M., Ahmad Hafiz, I., Kaleem, S., Qayyum, A., & Ahmad, M. (2012). In vitro selection of tissue culture induced somaclonal variants of wheat for drought tolerance. Journal of Agricultural Research (03681157), 50(2).
  • Murray, C. J., & Lopez, A. D. (1997). Alternative projections of mortality and disability by cause 1990–2020: Global Burden of Disease Study. The lancet, 349(9064), 1498-1504.
  • Mzabri, I., Addi, M., & Berrichi, A. (2019). Traditional and modern uses of saffron (Crocus sativus). Cosmetics, 6(4), 63.
  • Nath, A. K., Kumari, S. U. M. A. N., & Sharma, D. R. (2005). In vitro selection and characterization of water stress tolerant cultures of bell pepper. Indian Journal of Plant Physiology, 10(1), 14.
  • Orlikowska, T., Kucharska, D., & Horbowicz, M. (2008, September). The reaction of raspberry and blackberry cultivars to drought stress simulated in vitro by polyethylene glycol (PEG) 6000. In I International Symposium on Biotechnology of Fruit Species: Biotechfruit2008 839 (pp. 337-342).
  • Patade, V. Y., Bhargava, S., & Suprasanna, P. (2012). Effects of NaCl and iso-osmotic PEG stress on growth, osmolytes accumulation and antioxidant defense in cultured sugarcane cells. Plant Cell, Tissue and Organ Culture (PCTOC), 108(2), 279-286.
  • Piwowarczyk, B., Kamińska, I., & Rybiński, W. (2014). Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in Lathyrus culture. Czech Journal of Genetics and Plant Breeding, 50(2), 77-83.
  • Premkumar, K.; Abraham, S.K.; Santhiya, S.T.; Ramesh, A. Protective effects of saffron (Crocus sativus L.) on genotoxinsinduced oxidative stress in Swiss albino mice. Phytother. Res. 2003, 17, 614–617.
  • Priyanka, S., Rizwan, M., Bhatt, K. V., Mohapatra, T., & Govind, S. (2011). In-vitro response of Vigna aconitifolia to drought stress induced by peg 6000. Journal of Stress Physiology & Biochemistry, 7(3).
  • Rao, S., & Jabeen, F. T. Z. (2013). In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.). Physiology and Molecular Biology of Plants, 19(2), 261-268.
  • Saeedavi, L., Soleimani, A., & Amiri, M. E. (2017). Improvement of shoot-tip culture proliferation in banana using PEG 6000. Iranian Journal of Plant Physiology, 7(3).
  • Saepudin, A., Khumaida, N., Sopandie, D., & Ardie, S. W. (2017). In Vitro selection of four soybean genotypes using PEG for drought tolerance. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 45(1), 14-22.
  • Sharifi, G., Niknam, V., Sedighi, F., & Seifi Kalhor, M. 2020. Investigation of GABA effect on drought stress tolerance improvement in cultivated saffron (Crocus sativus L.). Journal of Plant Process and Function, vol. 9, no. 39.
  • Sharma, S., & Vakhlu, J. (2020). Callus induction and high frequency organogenesis in saffron (Crocus sativus L.). Applied Biological Research, 22(1), 61-68.
  • Sivritepe, N., Erturk, U., Yerlikaya, C., Turkan, I., Bor, M., & Ozdemir, F. (2008). Response of the cherry rootstock to water stress induced in vitro. Biologia Plantarum, 52(3), 573.
  • Smith, R. H., Bhaskaran, S., & Miller, F. R. (1985). Screening for drought tolerance in sorghum using cell culture. In Vitro Cellular amp; Developmental Biology, 21(10), 541-545.
  • Suganya, K., Preethi, P. S., Suganya, M., & Nanthini, A. U. R. (2016). Natural pigments in cosmetics-past to present. International Journal of Pharmaceutical Sciences and Business, 4, 07-14.
  • Taheri-Dehkordi, A., Naderi, R., Martinelli, F., & Salami, S. A. (2020). A robust workflow for indirect somatic embryogenesis and cormlet production in saffron (Crocus sativus L.) and its wild allies; C. caspius and C. speciosus. Heliyon, 6(12), e05841.
  • Tsago, Y., Andargie, M., & Takele, A. (2014). In vitro selection of sorghum (Sorghum bicolor (L) Moench) for polyethylene glycol (PEG) induced drought stress. Plant Science Today, 1(2), 62-68.
  • Turhan, H., & Baser, I. (2004). In vitro AND In vivo Water Stress In Sunflower (Helianthus Annuus L.)/Estrés Hídrico En Girasol (Helianthus annuus L.) En Las Condiciones In Vitro E In Vivo/Stress D’eau Du Tournesol (Helianthus annus L.) Dans Les Conditions In vitro Et In vivo. Helia, 27(40), 227-236.
  • Vahedi, M., Kalantari, S., & Salami, S. A. (2015). Effects of osmolytic agents on somatic embryogenesis of saffron (Crocus sativus L.). Notulae Scientia Biologicae, 7(1), 57-61.
  • Wani, S. H., Sofi, P. A., Gosal, S. S., & Singh, N. B. (2010). In vitro screening of rice (Oryza sativa L) callus for drought tolerance. Communications in Biometry and Crop Science, 5(2), 108-115.
  • Wu, X., Zeng, F., & Zhang, G. (2017). PEG-simulated drought stress and spike in vitro culture are used to study the impact of water stress on barley malt quality. Plant Growth Regulation, 81(2), 243-252.

FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ

Year 2021, Volume: 5 Issue: 2, 408 - 417, 31.10.2021

Abstract

Safran olarak bilinen Crocus sativus L., eski çağlardan beri kuru stigmaları baharat olarak kullanılan önemli bir bitkidir. Son zamanlarda abiyotik stres bitkisel üretimde karşılaşılan en önemli sorunlardan birisidir. Kuraklık stresi, bitkinin gelişimini farklı seviyelerde sınırlayan en önemli abiyotik stres olarak bilinmektedir. Kuraklık stresine dayanıklı bitkilerin seçilmesinde in vitro seleksiyon yöntemi oldukça önemli bir tekniktir. Polyetilen glikol (PEG), toksik olmayan ve bitkiye tutunmayan bir kimyasaldır ve genelde bitkilerin kuraklığa gösterdiği tepkileri ölçmek adına yapılan kuraklık stresi çalışmalarında simülatör olarak kullanılmaktadır. Bu çalışmada, Crocus sativus L., bitkisinin kormları 30 g L-1 sakkaroz, 2 mg L-1 2,4-D + 1 mg L-1 2iP, 7 g L-1 agar ve 0, 5%, 10%, 20% PEG 6000 ile kombine edilmiş MS besi yerinde kültüre alınmıştır. En etkili in vitro rejenerasyonun PEG içermeyen MS besi yerinde olduğu belirlenmiştir. Rejenere olan eksplantlar, sürgün oluşumu elde etmek için bitki büyüme düzenleyicisi içermeyen MS besi yerine transfer edilmiştir. Meydana gelen embryojenik yapıların farklı embriyo aşamalarında tutuklu kaldığı ve sürgün oluşmadığı gözlenmemiştir. Aynı besi yerinde organogenesis sonucu oluşan korm benzeri yapılarda da gelişim sınırlı kalmış ve bitkiye dönüşüm gerçekleşmemiştir.

References

  • Aazami, M. A., Torabi, M., & Jalili, E. (2010). In vitro response of promising tomato genotypes for tolerance to osmotic stress. African Journal of Biotechnology, 9(26), 4014-4017.
  • Ahmad, M. A., Javed, R., Adeel, M., Rizwan, M., & Yang, Y. (2020). PEG 6000-Stimulated Drought Stress Improves the Attributes of In Vitro Growth, Steviol Glycosides Production, and Antioxidant Activities in Stevia rebaudiana Bertoni. Plants, 9(11), 1552.
  • Algrech, C. (2001). The saffron of Quercy. Rev. Quercy Rech.,97, 20–27.
  • Aslezaeem, F., Niknam, V., Ebrahimzadeh, H., & Sharifi, G. (2018). Comparative study of drought stress and salicylic acid effects on different accessions of saffron (Crocus Sativus L.), 554-569.
  • Biswas, J., Chowdhury, B., Bhattacharya, A., & Mandal, A. B. (2002). In vitro screening for increased drought tolerance in rice. In Vitro Cellular & Developmental Biology-Plant, 38(5), 525-530.
  • Boris, P. M., Dubravka, Š., Ružica, Ž. P., Bojana, B., & Vladislava, G. Antioxidative Response Of Poplar Tissue Culture Exposed To Peg. 22nd International Symposium on Analytical and Environmental Problems,267-269.
  • Bouiamrine, E. H., & Diouri, M. (2012). Response of durum wheat (Triticum durum Desf.) callus culture to osmosis-induced drought stress caused by polyethylene glycol (PEG). Annals of Biological Research, 3(9), 4555-4563.
  • Dami, I., & Hughes, H. (1995). Leaf anatomy and water loss of in vitro PEG-treated ‘Valiant’grape. Plant cell, tissue and organ culture, 42(2), 179-184.
  • Dami, I., & Hughes, H. G. (1997). Effects of PEG-induced water stress on in vitro hardening of ‘Valiant’grape. Plant Cell, Tissue and Organ Culture, 47(2), 97-101.
  • Duncan, R. R., Waskom, R. M., & Nabors, M. W. (1995). In vitro screening and field evaluation of tissue-culture-regenerated sorghum (Sorghum bicolor (L.) Moench) for soil stress tolerance. In The Methodology of Plant Genetic Manipulation: Criteria for Decision Making (pp. 373-380). Springer, Dordrecht.
  • Dwyer, A., Hawrelak, J., Whitten, D.(2011). Herbal Medicines, other than St. John’s Wort, in the treatment of Depression: A systematic Review. Sci. Rev. Altern. Med. 16, 40–49.
  • Fallon, K. M., & Phillips, R. (1989). Responses to water stress in adapted and unadapted carrot cell suspension cultures. Journal of Experimental Botany, 40(6), 681-687.
  • Farooq M, Basra S. M. A., Wahid A., Cheema Z. A., Cheema M. A., & Khaliq A. (2008). Physiological role of exogenously applied glycinebetaine in improve drought tolerance of fine grain aromatic rice (Oryza sativa L.). J. Agron. Crop Sci, 194, 325-333.
  • Gao, H., Xu, D., Zhang, H., Cheng, X., & Yang, Q. (2020). Effects of culture medium composition and PEG on hyperhydricity in Dendrobium officinale. In Vitro Cellular & Developmental Biology-Plant, 56(2), 143-149.
  • Ghassemi-Golezani, K., Farhadi, N., & Nikpour-Rashidabad, N. (2018). Responses of in vitro-cultured Allium hirtifolium to exogenous sodium nitroprusside under PEG-imposed drought stress. Plant Cell, Tissue and Organ Culture (PCTOC), 133(2), 237-248.
  • Guclu, G., Kelebek, H., & Selli, S. (2020). Saffron (Crocus sativus L.): Its Aroma and Key Odorants. In Saffron (pp. 69-82). Academic Press.
  • Hajihashemi, S., & Ehsanpour, A. A. (2014). Antioxidant response of Stevia rebaudiana B. to polyethylene glycol and paclobutrazol treatments under in vitro culture. Applied biochemistry and biotechnology, 172(8), 4038-4052.
  • Hill, T. (2004). The contemporary encyclopedia of herbs & spices: seasonings for the global kitchen. J. Wiley.
  • Hongbo, S., Zongsuo, L., Mingan, S., & Bochu, W. (2005). Impacts of PEG-6000 pretreatment for barley (Hordeum vulgare L.) seeds on the effect of their mature embryo in vitro culture and primary investigation on its physiological mechanism. Colloids and Surfaces B: Biointerfaces, 41(2-3), 73-77.
  • Khazdair, M. R., Anaeigoudari, A., Hashemzehi, M., & Mohebbati, R. (2019). Neuroprotective potency of some spice herbs, a literature review. Journal of traditional and complementary medicine, 9(2), 98-105.
  • Mahmood, I., Razzaq, A., Ashraf, M., Ahmad Hafiz, I., Kaleem, S., Qayyum, A., & Ahmad, M. (2012). In vitro selection of tissue culture induced somaclonal variants of wheat for drought tolerance. Journal of Agricultural Research (03681157), 50(2).
  • Murray, C. J., & Lopez, A. D. (1997). Alternative projections of mortality and disability by cause 1990–2020: Global Burden of Disease Study. The lancet, 349(9064), 1498-1504.
  • Mzabri, I., Addi, M., & Berrichi, A. (2019). Traditional and modern uses of saffron (Crocus sativus). Cosmetics, 6(4), 63.
  • Nath, A. K., Kumari, S. U. M. A. N., & Sharma, D. R. (2005). In vitro selection and characterization of water stress tolerant cultures of bell pepper. Indian Journal of Plant Physiology, 10(1), 14.
  • Orlikowska, T., Kucharska, D., & Horbowicz, M. (2008, September). The reaction of raspberry and blackberry cultivars to drought stress simulated in vitro by polyethylene glycol (PEG) 6000. In I International Symposium on Biotechnology of Fruit Species: Biotechfruit2008 839 (pp. 337-342).
  • Patade, V. Y., Bhargava, S., & Suprasanna, P. (2012). Effects of NaCl and iso-osmotic PEG stress on growth, osmolytes accumulation and antioxidant defense in cultured sugarcane cells. Plant Cell, Tissue and Organ Culture (PCTOC), 108(2), 279-286.
  • Piwowarczyk, B., Kamińska, I., & Rybiński, W. (2014). Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in Lathyrus culture. Czech Journal of Genetics and Plant Breeding, 50(2), 77-83.
  • Premkumar, K.; Abraham, S.K.; Santhiya, S.T.; Ramesh, A. Protective effects of saffron (Crocus sativus L.) on genotoxinsinduced oxidative stress in Swiss albino mice. Phytother. Res. 2003, 17, 614–617.
  • Priyanka, S., Rizwan, M., Bhatt, K. V., Mohapatra, T., & Govind, S. (2011). In-vitro response of Vigna aconitifolia to drought stress induced by peg 6000. Journal of Stress Physiology & Biochemistry, 7(3).
  • Rao, S., & Jabeen, F. T. Z. (2013). In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.). Physiology and Molecular Biology of Plants, 19(2), 261-268.
  • Saeedavi, L., Soleimani, A., & Amiri, M. E. (2017). Improvement of shoot-tip culture proliferation in banana using PEG 6000. Iranian Journal of Plant Physiology, 7(3).
  • Saepudin, A., Khumaida, N., Sopandie, D., & Ardie, S. W. (2017). In Vitro selection of four soybean genotypes using PEG for drought tolerance. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 45(1), 14-22.
  • Sharifi, G., Niknam, V., Sedighi, F., & Seifi Kalhor, M. 2020. Investigation of GABA effect on drought stress tolerance improvement in cultivated saffron (Crocus sativus L.). Journal of Plant Process and Function, vol. 9, no. 39.
  • Sharma, S., & Vakhlu, J. (2020). Callus induction and high frequency organogenesis in saffron (Crocus sativus L.). Applied Biological Research, 22(1), 61-68.
  • Sivritepe, N., Erturk, U., Yerlikaya, C., Turkan, I., Bor, M., & Ozdemir, F. (2008). Response of the cherry rootstock to water stress induced in vitro. Biologia Plantarum, 52(3), 573.
  • Smith, R. H., Bhaskaran, S., & Miller, F. R. (1985). Screening for drought tolerance in sorghum using cell culture. In Vitro Cellular amp; Developmental Biology, 21(10), 541-545.
  • Suganya, K., Preethi, P. S., Suganya, M., & Nanthini, A. U. R. (2016). Natural pigments in cosmetics-past to present. International Journal of Pharmaceutical Sciences and Business, 4, 07-14.
  • Taheri-Dehkordi, A., Naderi, R., Martinelli, F., & Salami, S. A. (2020). A robust workflow for indirect somatic embryogenesis and cormlet production in saffron (Crocus sativus L.) and its wild allies; C. caspius and C. speciosus. Heliyon, 6(12), e05841.
  • Tsago, Y., Andargie, M., & Takele, A. (2014). In vitro selection of sorghum (Sorghum bicolor (L) Moench) for polyethylene glycol (PEG) induced drought stress. Plant Science Today, 1(2), 62-68.
  • Turhan, H., & Baser, I. (2004). In vitro AND In vivo Water Stress In Sunflower (Helianthus Annuus L.)/Estrés Hídrico En Girasol (Helianthus annuus L.) En Las Condiciones In Vitro E In Vivo/Stress D’eau Du Tournesol (Helianthus annus L.) Dans Les Conditions In vitro Et In vivo. Helia, 27(40), 227-236.
  • Vahedi, M., Kalantari, S., & Salami, S. A. (2015). Effects of osmolytic agents on somatic embryogenesis of saffron (Crocus sativus L.). Notulae Scientia Biologicae, 7(1), 57-61.
  • Wani, S. H., Sofi, P. A., Gosal, S. S., & Singh, N. B. (2010). In vitro screening of rice (Oryza sativa L) callus for drought tolerance. Communications in Biometry and Crop Science, 5(2), 108-115.
  • Wu, X., Zeng, F., & Zhang, G. (2017). PEG-simulated drought stress and spike in vitro culture are used to study the impact of water stress on barley malt quality. Plant Growth Regulation, 81(2), 243-252.
There are 43 citations in total.

Details

Primary Language Turkish
Subjects Ecology, Forest Industry Engineering
Journal Section Research Article
Authors

Başar Sevindik

Publication Date October 31, 2021
Published in Issue Year 2021 Volume: 5 Issue: 2

Cite

APA Sevindik, B. (2021). FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ. Turkish Journal of Forest Science, 5(2), 408-417.
AMA Sevindik B. FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ. Turk J For Sci. October 2021;5(2):408-417.
Chicago Sevindik, Başar. “FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ”. Turkish Journal of Forest Science 5, no. 2 (October 2021): 408-17.
EndNote Sevindik B (October 1, 2021) FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ. Turkish Journal of Forest Science 5 2 408–417.
IEEE B. Sevindik, “FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ”, Turk J For Sci, vol. 5, no. 2, pp. 408–417, 2021.
ISNAD Sevindik, Başar. “FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ”. Turkish Journal of Forest Science 5/2 (October 2021), 408-417.
JAMA Sevindik B. FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ. Turk J For Sci. 2021;5:408–417.
MLA Sevindik, Başar. “FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ”. Turkish Journal of Forest Science, vol. 5, no. 2, 2021, pp. 408-17.
Vancouver Sevindik B. FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ. Turk J For Sci. 2021;5(2):408-17.