Osmaniye Korkut Ata University Journal of the Institute of Science and Technology

Abstract

Orchid seeds are transparent and embryos can be seen easily. In our previous study, embryos were isolated from transparent seeds using the maceration technique with different enzymes. In this publication, it is aimed to create a simple embryo isolation method by using chloralhydrate and similar different clearing and tissue expanding chemicals with a simpler method. Two different methods were used in the study. In the first method, dissected orchid ovules were kept at different times in a mixture of lactic acid, chloralhydrate, phenol, clove oil and xylol at different rates. In the other method, ovules were kept in chloralhydrate solution for 3 different times, 24 hours, 48 hours and 72 hours. In both methods, it was tested whether the embryos could easily come out of the ovules with a slight pressure, and also the fertility of the methods was compared with each other according to the integrity or fragmentation of the embryos that came out of the ovules with pressure. As a result; It has been determined that this method is more efficient in terms of comfortable isolation of embryos from ovules kept in chloral hydrate for 72 hours and in terms of integrity and robustness of embryos. In addition, with the efficiency of this method, it has been observed that the embryos and suspensor structures exit the ovules in a very easy and practical way. In addition, it has been understood that the method will accelerate the studies of funiculus, placenta, carpel, megasposorist-archaeosporal cell and ovule development. Thus, it has been possible to examine some important embryonal and suspensory structures practically and quickly without the need for long-term embedding and histological sections in the microtome. The benefits of the method and its inadequacies, albeit partially, are discussed in the presence of various current articles.