Research Article
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Doğadan toplanan Tubifex tubifex’ in mikrobiyal yük seviyesinin kontrollü laboratuvar şartlarında değişimi

Year 2020, Volume: 2 Issue: 2, 71 - 77, 31.12.2020

Abstract

Bu çalışmada, doğadan toplanan Tubifex tubifex’ler ile 28 gün boyunca kontrollü şartlarda tutulan T. tubifex’lerin mikrobiyolojik yük sevileri karşılaştırılmıştır. Bu amaçla altı farklı deneme grubu oluşturulmuştur. Bu gruplar (1) tank zemininde kum olan ve ultaviole (UV) sterilizasyon işlemi uygulanan grup (1. Grup = KUVL),
(2) tank zemininde kum olan ancak UV sterilizasyon uygulanmayan grup (2. Grup = KUVS), (3) tank zemininde çamur olan ve UV sterilizasyon işlemi uygulanan grup (3. Grup = ÇUVL), (4) tank zemininde çamur olan ancak UV sterilizasyon uygulanmayan grup (4. Grup = ÇUVS), (5) tank zemini boş olan ve UV sterilizasyon işlemi uygulanan grup (5. Grup = ZUVL), (6) tank zemini boş olan ancak UV sterilizasyon işlemi uygulanmayan grup (6. Grup = ZUVS) olarak planlanmıştır. Su parametreleri tüm gruplarda sabit tutulmuştur. Denemeler üç tekerrürlü olacak şekilde gerçekleştirilmiş olup her bir gruba 100’er gram canlı T. tubifex stoklanmıştır. Kapalı devre sistemde bir yirmisekiz gün boyunca tutulan T. tubifex’ lerden periyodik olarak (0. gün, 7. gün, 14. gün, 21. gün ve 28. gün) mikrobiyolojik örnekler alınmış ve ölçümler yapılmıştır. Elde edilen bulgulara göre toplam aerob bakteri sayısı (TAB) tüm gruplarda zamana bağlı bir azalma göstermiştir. Bunun yanı sıra ortamdaki mikrobiyolojik bakteri yük miktarının başlangıç anından itibaren 28 günün sonuna kadar sürekli düşüş eğilimi gösterdiği tespit edilmiştir.

Supporting Institution

TÜBİTAK

Project Number

213O033

Thanks

Bu çalışma 213O033 nolu TUBİTAK projesi kapsamında desteklenmiştir

References

  • Beauchamp, K. A., Gay, M., Kelley, G. O., El-Matbouli, M., Kathman, R. D., Nehring, R. B. & Hedrick, R. P. (2002). Prevalence and susceptibility of infection to Myxobolus cerebralis and genetic differences among populations of Tubifex tubifex. Diseases of Aquatic Organisms, 51: 113-121.
  • Beauchamp, K. A., El-Matbouli, M., Gay, M., Georgiadis, M. P., Nehring, R. B. & Hedrick, R. P. (2006). The effect of cohabitation of Tubifex tubifex (Oligochaeta: Tubificidae) populations on infections to Myxobolus cerebralis (Myxozoa: Myxobolidae). Journal of Invertebrate Pathology, 91(1): 1-8.
  • Brinkhurst, R. O. & Jamieson, B. G. M. (1971). Aquatic Oligochaeta of the world. Oliver and Boyd, Edinburgh.
  • Chekanovskaya, O. V. (1981). Aquatic Oligochaeta of the USSR. Amerind Publishing Company. New Delhi.
  • Çelik, P. (2018). Sediment yapısının Tubifex tubifex’in biyokimyasal kompozisyonu üzerine etkisi. International Eurasian Conference on Biological and chemical Sciences, 26-27 April 2018, Ankara. Proceeding Book 129-134 pp.
  • Elibol, M. İ., Üstündağ, S. & Çevlik, H. (2006). Eğrekkaya Baraj Gölü limnolojisi, I. Balıklandırma ve Rezervuar Yönetimi Sempozyumu, Antalya, pp:447-452.
  • El-Mansy, A. (1998). Development of Myxobolus portucalensis Saraiva and Molnár, 1990 (Myxosporea: Myxobolidae) in the oligochaete Tubifex tubifex (Müller). Systematic Parasitology, 41(2): 95-103.
  • El-Matbouli, M. & Hoffman, R. W. (1989). Experimental transmission of two Myxobolus spp. developing bisporogeny via Tubificid Oligochaetes. Parasitology Research, 75: 461-464.
  • El-Matbouli, M. & Hoffman, R. W. (1995). Light and electron microscopic observations on the route of the triactinomyxonssporoplasm of Myxobolus cerebralis from epidermis into Rainbow trout cartilage. Journal of Fish Biology, 46: 919-935.
  • Eszterbauer, E., Székely, C., Molnár, K. & Baska, F. (2000). Development of Myxobolus bramae (Myxosporea: Myxobolidae) in an oligochaete alternate host, Tubifex tubifex. Journal of Fish Diseases, 23(1): 19-25.
  • Famme, P. & Knudsen, J. (1984). Total heat balance study of anaerobiosis in Tubifex tubifex (Müller). Journal of Comparative Physiology B, 154(6): 587-591.
  • Kaeser, A. J. & Sharpe, W. E. (2006). Patterns of distribution and abundance of Tubifex tubifex nd other aquatic oligochaetes in Myxobolus cerebralis enzootic areas in Pennsylvania. Journal of Aquatic Animal Health, 18(1): 64-78.
  • Lang, C. & Lang-Dobler, B. (1980). Structure of tubificid and lumbriculid communities, and three indices of trophy based upon these communities, as descriptors of eutrophication level of Lake Geneva (Switzerland). In R. O. Brinkhurst & D. G. Cook (eds), Aquatic Oligochaete Biology. Plenum Publishing Corporation, New York: 457–470.
  • Lazim, M. N. & Learner, M. A. (1986). The life‐cycle and productivity of Tubifex tubifex (Oligochaeta; Tibificidae) in the Moat-Feeder Stream, Cardiff, South Wales. Ecography, 9(3): 185-192. Markiw, M. E. & Wolf, K. (1983). Myxosoma cerebralis (Myxozoa: Myxosporea) etiologic agent of salmonid whirling disease requires Tubificid Oligochaetes (Annelida: Oligochaetes) in its life cycle. The Journal of Protozoology, 30: 561-564.
  • Milbrink, G. (1978). Indicator communities of oligochaetes in Scandinavian lakes. Internationale Vereinigung für theoretische und angewandte Limnologie, Verhandlungen, 20(4): 2406-2411.
  • Milbrink, G. (1980). Oligochaete communities in pollution biology. The European situation with special reference to lakes in Scandinavia. In R. O. Brinkhurst & D. G. Cook (eds), Aquatic Oligochaete Biology. Plenum Publishing Corporation, New York: 433–455.
  • Milbrink, G. (1987). Biological characterization of sediments by standardized tubificid bioassays. Hydrobiologia, 155: 267-275.
  • Peters, W. (1977). Possible sites of ultrafiltration in Tubifex tubifex Müller (Annelida, Oligochaeta). Cell and Tissue Research, 179(3): 367-375.
  • Sarkka, J. (1987). The occurrence of oligochaetes in lake chains receiving pulp mill waste and their relation to eutrophication on the trophic scale. Hydrobiologia, 155: 259-265.
  • Steen Redeker, E., Bervoets, L. & Blust, R. (2004). Dynamic model for the accumulation of cadmium and zinc from water and sediment by the aquatic oligochaete, Tubifex tubifex. Environmental Science and Technology, 38(23): 6193-6200.
  • Şahin, S. K. & Yıldız, S. (2011). Species distribution of oligochaetes related to environmental parameters in Lake Sapanca (Marmara Region, Turkey), Turkish Journal of Fisheries and Aquatic Sciences, 11(3): 359-366.
  • Tanyolaç, J. (1993). Limnoloji, Hatiboğlu Yayınları, Ankara, pp: 294.
  • Wolf, K., Markiw, M. E. & Hiltunen, J. K. (1986). Salmonid whirling disease: Tubifex tubifex (Müller) identified as the essential oligochaete in the protozoan life cycle. Journal of Fish Diseases, 9(1): 83-85.
  • Yanar, M., Yanar, Y. & Genç, M. A. (2003). Tubifex tubifex Müller, 1774 (Annelidae)’in Besin Kompozisyonu, Ege Üniversitesi Su Ürünleri Dergisi, 20(1-2): 103-110.

Temporal variation of microbial load under controlled laboratory conditions of Tubifex tubifex collected from nature

Year 2020, Volume: 2 Issue: 2, 71 - 77, 31.12.2020

Abstract

This paper has compared microbiological load levels of Tubifex tubifex both collected from nature and kept under controlled conditions for 28 days. Six different experimental groups were investigated. These groups were (1) the group with sand on the tank floor and ultraviolet (UV) sterilized (Group 1 = KUVL), (2) the group with sand on the tank floor but not UV sterilized (Group 2 = KUVS), (3) the group with mud on the tank floor and UV sterilized (Group 3 = ÇUVL), (4) the group with mud on the tank floor but not UV sterilized (Group
4= ÇUVS), (5) the group with the empty tank floor and UV sterilized (Group 5 = ZUVL), (6) the group with the empty tank floor but not UV sterilized (Group 6 = ZUVS). Water-related parameters were kept constant in all groups. The experiments were carried out in triplicate and 100 grams of alive T. tubifex were stocked for each group. Microbiological samples were taken from T. tubifex, which were kept in the closed-circuit system for twenty-eight days, on a weekly basis periodically (0, 7, 14, 21, 28 days) and measurements were conducted.
The results revealed that the number of total aerobic bacteria (TAB) showed a time-dependent decrease in all groups. Moreover, it has been determined that the amount of microbiological bacterial load decreased continuously until the end of 28 days.

Project Number

213O033

References

  • Beauchamp, K. A., Gay, M., Kelley, G. O., El-Matbouli, M., Kathman, R. D., Nehring, R. B. & Hedrick, R. P. (2002). Prevalence and susceptibility of infection to Myxobolus cerebralis and genetic differences among populations of Tubifex tubifex. Diseases of Aquatic Organisms, 51: 113-121.
  • Beauchamp, K. A., El-Matbouli, M., Gay, M., Georgiadis, M. P., Nehring, R. B. & Hedrick, R. P. (2006). The effect of cohabitation of Tubifex tubifex (Oligochaeta: Tubificidae) populations on infections to Myxobolus cerebralis (Myxozoa: Myxobolidae). Journal of Invertebrate Pathology, 91(1): 1-8.
  • Brinkhurst, R. O. & Jamieson, B. G. M. (1971). Aquatic Oligochaeta of the world. Oliver and Boyd, Edinburgh.
  • Chekanovskaya, O. V. (1981). Aquatic Oligochaeta of the USSR. Amerind Publishing Company. New Delhi.
  • Çelik, P. (2018). Sediment yapısının Tubifex tubifex’in biyokimyasal kompozisyonu üzerine etkisi. International Eurasian Conference on Biological and chemical Sciences, 26-27 April 2018, Ankara. Proceeding Book 129-134 pp.
  • Elibol, M. İ., Üstündağ, S. & Çevlik, H. (2006). Eğrekkaya Baraj Gölü limnolojisi, I. Balıklandırma ve Rezervuar Yönetimi Sempozyumu, Antalya, pp:447-452.
  • El-Mansy, A. (1998). Development of Myxobolus portucalensis Saraiva and Molnár, 1990 (Myxosporea: Myxobolidae) in the oligochaete Tubifex tubifex (Müller). Systematic Parasitology, 41(2): 95-103.
  • El-Matbouli, M. & Hoffman, R. W. (1989). Experimental transmission of two Myxobolus spp. developing bisporogeny via Tubificid Oligochaetes. Parasitology Research, 75: 461-464.
  • El-Matbouli, M. & Hoffman, R. W. (1995). Light and electron microscopic observations on the route of the triactinomyxonssporoplasm of Myxobolus cerebralis from epidermis into Rainbow trout cartilage. Journal of Fish Biology, 46: 919-935.
  • Eszterbauer, E., Székely, C., Molnár, K. & Baska, F. (2000). Development of Myxobolus bramae (Myxosporea: Myxobolidae) in an oligochaete alternate host, Tubifex tubifex. Journal of Fish Diseases, 23(1): 19-25.
  • Famme, P. & Knudsen, J. (1984). Total heat balance study of anaerobiosis in Tubifex tubifex (Müller). Journal of Comparative Physiology B, 154(6): 587-591.
  • Kaeser, A. J. & Sharpe, W. E. (2006). Patterns of distribution and abundance of Tubifex tubifex nd other aquatic oligochaetes in Myxobolus cerebralis enzootic areas in Pennsylvania. Journal of Aquatic Animal Health, 18(1): 64-78.
  • Lang, C. & Lang-Dobler, B. (1980). Structure of tubificid and lumbriculid communities, and three indices of trophy based upon these communities, as descriptors of eutrophication level of Lake Geneva (Switzerland). In R. O. Brinkhurst & D. G. Cook (eds), Aquatic Oligochaete Biology. Plenum Publishing Corporation, New York: 457–470.
  • Lazim, M. N. & Learner, M. A. (1986). The life‐cycle and productivity of Tubifex tubifex (Oligochaeta; Tibificidae) in the Moat-Feeder Stream, Cardiff, South Wales. Ecography, 9(3): 185-192. Markiw, M. E. & Wolf, K. (1983). Myxosoma cerebralis (Myxozoa: Myxosporea) etiologic agent of salmonid whirling disease requires Tubificid Oligochaetes (Annelida: Oligochaetes) in its life cycle. The Journal of Protozoology, 30: 561-564.
  • Milbrink, G. (1978). Indicator communities of oligochaetes in Scandinavian lakes. Internationale Vereinigung für theoretische und angewandte Limnologie, Verhandlungen, 20(4): 2406-2411.
  • Milbrink, G. (1980). Oligochaete communities in pollution biology. The European situation with special reference to lakes in Scandinavia. In R. O. Brinkhurst & D. G. Cook (eds), Aquatic Oligochaete Biology. Plenum Publishing Corporation, New York: 433–455.
  • Milbrink, G. (1987). Biological characterization of sediments by standardized tubificid bioassays. Hydrobiologia, 155: 267-275.
  • Peters, W. (1977). Possible sites of ultrafiltration in Tubifex tubifex Müller (Annelida, Oligochaeta). Cell and Tissue Research, 179(3): 367-375.
  • Sarkka, J. (1987). The occurrence of oligochaetes in lake chains receiving pulp mill waste and their relation to eutrophication on the trophic scale. Hydrobiologia, 155: 259-265.
  • Steen Redeker, E., Bervoets, L. & Blust, R. (2004). Dynamic model for the accumulation of cadmium and zinc from water and sediment by the aquatic oligochaete, Tubifex tubifex. Environmental Science and Technology, 38(23): 6193-6200.
  • Şahin, S. K. & Yıldız, S. (2011). Species distribution of oligochaetes related to environmental parameters in Lake Sapanca (Marmara Region, Turkey), Turkish Journal of Fisheries and Aquatic Sciences, 11(3): 359-366.
  • Tanyolaç, J. (1993). Limnoloji, Hatiboğlu Yayınları, Ankara, pp: 294.
  • Wolf, K., Markiw, M. E. & Hiltunen, J. K. (1986). Salmonid whirling disease: Tubifex tubifex (Müller) identified as the essential oligochaete in the protozoan life cycle. Journal of Fish Diseases, 9(1): 83-85.
  • Yanar, M., Yanar, Y. & Genç, M. A. (2003). Tubifex tubifex Müller, 1774 (Annelidae)’in Besin Kompozisyonu, Ege Üniversitesi Su Ürünleri Dergisi, 20(1-2): 103-110.
There are 24 citations in total.

Details

Primary Language Turkish
Subjects Hydrobiology, Fisheries Management
Journal Section Research Articles
Authors

Pinar Çelik 0000-0002-4417-3574

Fikret Çakır 0000-0001-5261-2365

Project Number 213O033
Publication Date December 31, 2020
Submission Date August 27, 2020
Acceptance Date October 19, 2020
Published in Issue Year 2020 Volume: 2 Issue: 2

Cite

APA Çelik, P., & Çakır, F. (2020). Doğadan toplanan Tubifex tubifex’ in mikrobiyal yük seviyesinin kontrollü laboratuvar şartlarında değişimi. Marine and Life Sciences, 2(2), 71-77.

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