Abstract
Kanser, insan sağlığı için en büyük tehditlerden birini oluşturmaktadır. İlaç direncinin kanser tedavisindeki temel sorunlardan biri olduğu düşünüldüğünde, doğal bileşiklerin kanser hücreleri üzerindeki etkilerine yönelik çalışmalar hızla artmaktadır. Yenilebilir mantarlar besin değerleri bakımından zengin olmaları nedeniyle fonksiyonel ve sağlıklı gıda kaynağı olarak değerlendirilmektedir. Yapılan çalışmalarda antikanser, anti-inflamatuvar, antioksidan ve antimikrobiyal etkiye sahip oldukları tespit edilmiştir. Ülkemizin birçok bölgesi ve Elazığ ilinde doğal olarak yetişen Tricholoma fracticum (Britzelm.) Kreisel besin olarak tüketilen yenilebilir bir mantardır. Bu çalışmada T. fracticum’un farklı konsantrasyonlardaki (1000µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL ve 62.5 µg/mL) metanol ekstresinin 24 ve 48 saatte Hep3B hücrelerine karşı sitotoksik etkisinin belirlenmesi amaçlanmıştır. Sitotoksik etkinin tespit edilmesi için 3-(4,5dimetiltiazol-2-iyl)-2,5-difenil tetrazolyum bromür (MTT) yöntemi kullanılmıştır. Elde edilen sonuçlarda T. fracticum’un metanol ekstresi 1000 µg/mL konsantrasyonda 24. (%1.97±0.65) ve 48. saatte (%0.61±0.27) önemli sitotoksik etki göstermiştir. Mantarın metanol ektresinin Hep3B hücre hattına karşı 24. saatte IC50 değerinin ise 166.76±7.65 µg/mL ve 48. saatte 134.38±5.45 µg/mL olarak hesaplanmıştır. T. fracticum ekstresinin Hep3B hücre hattına karşı elde edilen sitotoksik sonuçlarının önemli olabileceğini ve Tricholoma türleri arasında daha az çalışılan bu türün daha fazla çalışılması gerektiğini ve literatüre kazandırılması gerektiğini düşünmekteyiz.
Keywords
Tricholomataceae Tricholoma fracticum Tıbbi Mantar Hep3B insan hepatomu Sitotoksik etki MTT
References
- Akyüz, M., İnci, Ş., and Kırbağ, S. (2023). Evaluation of Antimicrobial, Antioxidant, Cytotoxic and DNA Protective Effects of Oyster Mushroom: Pleurotus pulmonarius (Fr.) Quel. Arabian J. Sci. Engineering, 48(6), 7273-7283.
- Anwanwan, D., Singh, S. K., Singh, S., Saikam, V., and Singh, R. (2020). Challenges in liver cancer and possible treatment approaches. Biochimica et Biophysica Acta, 1873(1), 188314.
- Center, M.M., and Jemal, A. (2011). International trends in liver cancer incidence rates. Cancer Epidemiol. Biomarkers & Prevention, 20(11), 2362-2368.
- Çınar, İ., Yayla, M., ve Binnetoğlu, D. (2020). Gossypinin insan hepatom (Hep-3B) hücreleri üzerindeki anti-proliferatif etkisi. Çukurova Med. J., 45(3), 1165-1172.
- Doğan, H. H., Kars, M. D., Özdemir, Ö., and Gündüz, U. (2020). Fomes fomentarius and Tricholoma anatolicum (Agaricomycetes) extracts exhibit significant multiple drug-resistant modulation activity in drug-resistant breast cancer cells. Int. J. Med. Mushrooms, 22(2).
- Erol, E., Ali, Z., Oztürk, M., Wang, M., and Khan, I.A. (2018). Phytochemical study on Tricholoma fracticum (Britzelm.) Kreisel. 18. Annual Oxford International Conference on the Science of Botanicals, MS/USA.
- Garlaschelli, L., Pang, Z.J., Sterner, O., Vidari, G. (1994) New indole derivatives from the fruit bodies of Tricholoma sciodes and T. virgatum. Tetrahedron, 50, 3571.
- Li, X., Lu, Y., Zhang, W., Yuan, S., Zhou, L., Wang, L., Ding, Q., Wang, D., Yang, W., and Chen, Y. (2016). Antioxidant capacity and cytotoxicity of sulfated polysaccharide TLH-3 from Tricholoma lobayense. Int. J. Biol. Macromolecules, 82, 913-919.
- Li, Y., Li, S., Meng, X., Gan, R. Y., Zhang, J. J., and Li, H. B. (2017). Dietary natural products for prevention and treatment of breast cancer. Nutrients, 9(7), 728.
- Liu, K., Chen, W., Zhou, Y., Xu, L., Sun, X., Mao, Y., and Ye, D. (2023). Associations between food groups and liver cancer: a systematic review and meta-analysis of observational studies. Nutrition J., 22(1), 1-12.
- Maiuolo, J., Gliozzi, M., Carresi, C., Musolino, V., Oppedisano, F., Scarano, F., Nucera, S., Scicchitano, M., Bosco, F., Macri, R., Ruga, S., Cardamone, A., Coppoletta, A., Mollace, A., Cognetti, F., and Mollace, V. (2021). Nutraceuticals and cancer: Potential for natural polyphenols. Nutrients, 13(11), 3834.
- Malheiro, R., de Pinho, P. G., Soares, S., da Silva Ferreira, A. C., and Baptista, P. (2013). Volatile biomarkers for wild mushrooms species discrimination. Food Res. Int., 54(1), 186-194.
- Mossman T., (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65:55-3.
- Ohnuma, N., Amemiya, K., Kakuda, R., Yaoita, Y., Machida, K., and Kikuchi, M. (2000) Sterol constituents from two edible mushrooms, Lentinula edodes and Tricholoma matsutake. Chem. Pharm. Bull., 48, 749–751
- Öztürk, C., ve Atila, F. (2021). Mantarların biyolojik aktiviteleri ile ilgili in vitro, in vivo ve klinik değerlendirmeler. J. Fac. Pharmacy of Ankara Uni., 45(2), 344-378.
- Pachon-Pena, G., Reyes-Zurita, F. J., Deffieux, G., Azqueta, A., de Cerain, A.L., Centelles, J.J., Creppy, E.E., and Cascante, M. (2009). Antiproliferative effect of flavomannin-6, 6′-dimethylether from Tricholoma equestre on Caco-2 cells. Toxicol., 264(3), 192-197.
- Sadi, G., Emsen, B., Kaya, A., Kocabaş, A., Çınar, S., and Kartal, D.I. (2015). Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties. Pharmacognosy magazine, 11(Suppl 1), 6.
- Sakai, S., Tomomura, Y., Yoshida, H., Inoue, S., and Kawagishi, H. (2005) Orirubenones D to G, novel phenones from the Tricholoma orirubens mushroom. Biosci. Biotechnol. Biochem., 69, 1630– 1632
- Sesli, E., Asan, A. ve Selçuk, F. (edlr.) Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu.,Yoltaş, A. (2020). Türkiye Mantarları Listesi. İstanbul: Ali Nihat Gökyiğit Vakfı Yayınları.
- Shi, C., Peng, Y. L., He, J., Li, Z. H., Liu, J.K., and Feng, T. (2021). Structures, chemical conversions, and cytotoxicity of tricholopardins C and D, two Tricholoma triterpenoids from the wild mushroom Tricholoma pardinum. Nat. Prod.and Bioprospecting, 11, 235-241.
- Solak, M.H., Işıloğlu, M., Kalmış, E., and Allı H., (2007). Macrofungi of Turkey, checklist .Vol. I, Genç Üniversiteliler Ofset, p: 254, Bornova, İzmir.
- Tel, G., Apaydın, M., Duru, M. E., and Öztürk, M. (2012). Antioxidant and cholinesterase inhibition activities of three Tricholoma species with total phenolic and flavonoid contents: the edible mushrooms from Anatolia. Food Anal. Methods, 5, 495-504.
- Yamaç, M., and Bilgili, F. (2006). Antimicrobial activities of fruit bodies and/or mycelial cultures of some mushroom isolates. Pharm. Biol., 44(9), 660-667.
- Tsukamoto, S., Macabalang, A.D., Nakatani, K., Obara, Y., Nakahata, N., and Ohta, T. (2003) Tricholoma lides A–C, new neurotrophic diterpenes from the mushroom Tricholoma sp. J. Nat. Prod., 66, 1578–1581
- Yoshikawa, K., Kuroboshi, M., Ahagon, S., and Arihara, S. (2004) Three novel crustulinol esters, saponaceols A–C, from Tricholoma saponaceum. Chem. Pharm. Bull., 52, 886–888
- Yılmaz, H.K., ve Özer, R.M. (2022). Ketojenik diyet ve kanser. Haliç Üni. Sağlık Bilimleri Derg., 5(1), 11-19.
- Zhang, A.L., Li, Y.L., Ma, Y.T., Gao, J.M., and Jia, A.Q. (2009) Phenolic meroterpenoids and steroids from the basidiomycete Tricholoma imbricatum. Biochem. Systematics Ecol., 37, 756–758
- Zhou, Y., Li, Y., Zhou, T., Zheng, J., Li, S., and Li, H.B. (2016). Dietary natural products for prevention and treatment of liver cancer. Nutrients, 8(3), 156.
- Wang, H. X., Liu, W. K., Ng, T.B., Ooi, V.E.C., and Chang, S.T. (1995). Immunomodulatory and antitumor activities of a polysaccharide-peptide complex from a mycelial culture of Tricholoma sp., a local edible mushroom. Life Sci., 57(3), 269-281.
Abstract
Cancer constitutes one of the greatest threats to human health. Considering that drug resistance is one of the main problems in cancer treatment, studies on the effects of natural compounds on cancer cells are increasing rapidly. Edible mushrooms are considered as a functional and healthy food source because they are rich in nutritional values. Studies have shown that they have anticancer, anti-inflammatory, antioxidant, and antimicrobial effects. Tricholoma fracticum (Britzelm.) Kreisel, which grows naturally in many regions of our country and in the province of Elazığ, is an edible mushroom consumed as food. In this study, it was aimed to determine the cytotoxic effect of methanol extract of T. fracticum at different concentrations (1000µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL and 62.5 µg/mL) against Hep3B cells at 24 and 48 hours. 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method was used to determine the cytotoxic effect. In the results obtained, the methanol extract of T. fracticum showed a significant cytotoxic effect at a concentration of 1000 µg/mL at the 24th (1.97±0.65%) and 48th hour (0.61±0.27%). The IC50 value of the methanol extract of the fungus against the Hep3B cell line was calculated as 166.76±7.65 µg/mL at 24 hours and 134.38±5.45 µg/mL at 48 hours. We think that the cytotoxic results of T. fracticum extract against Hep3B cell line may be important and this species, which is less studied among Tricholoma species, should be studied more and brought to the literatüre.
Keywords
Tricholomataceae Tricholoma fracticum Medicinal mushroom Hep3B human hepatoma Cytotoxic effect MTT
References
- Akyüz, M., İnci, Ş., and Kırbağ, S. (2023). Evaluation of Antimicrobial, Antioxidant, Cytotoxic and DNA Protective Effects of Oyster Mushroom: Pleurotus pulmonarius (Fr.) Quel. Arabian J. Sci. Engineering, 48(6), 7273-7283.
- Anwanwan, D., Singh, S. K., Singh, S., Saikam, V., and Singh, R. (2020). Challenges in liver cancer and possible treatment approaches. Biochimica et Biophysica Acta, 1873(1), 188314.
- Center, M.M., and Jemal, A. (2011). International trends in liver cancer incidence rates. Cancer Epidemiol. Biomarkers & Prevention, 20(11), 2362-2368.
- Çınar, İ., Yayla, M., ve Binnetoğlu, D. (2020). Gossypinin insan hepatom (Hep-3B) hücreleri üzerindeki anti-proliferatif etkisi. Çukurova Med. J., 45(3), 1165-1172.
- Doğan, H. H., Kars, M. D., Özdemir, Ö., and Gündüz, U. (2020). Fomes fomentarius and Tricholoma anatolicum (Agaricomycetes) extracts exhibit significant multiple drug-resistant modulation activity in drug-resistant breast cancer cells. Int. J. Med. Mushrooms, 22(2).
- Erol, E., Ali, Z., Oztürk, M., Wang, M., and Khan, I.A. (2018). Phytochemical study on Tricholoma fracticum (Britzelm.) Kreisel. 18. Annual Oxford International Conference on the Science of Botanicals, MS/USA.
- Garlaschelli, L., Pang, Z.J., Sterner, O., Vidari, G. (1994) New indole derivatives from the fruit bodies of Tricholoma sciodes and T. virgatum. Tetrahedron, 50, 3571.
- Li, X., Lu, Y., Zhang, W., Yuan, S., Zhou, L., Wang, L., Ding, Q., Wang, D., Yang, W., and Chen, Y. (2016). Antioxidant capacity and cytotoxicity of sulfated polysaccharide TLH-3 from Tricholoma lobayense. Int. J. Biol. Macromolecules, 82, 913-919.
- Li, Y., Li, S., Meng, X., Gan, R. Y., Zhang, J. J., and Li, H. B. (2017). Dietary natural products for prevention and treatment of breast cancer. Nutrients, 9(7), 728.
- Liu, K., Chen, W., Zhou, Y., Xu, L., Sun, X., Mao, Y., and Ye, D. (2023). Associations between food groups and liver cancer: a systematic review and meta-analysis of observational studies. Nutrition J., 22(1), 1-12.
- Maiuolo, J., Gliozzi, M., Carresi, C., Musolino, V., Oppedisano, F., Scarano, F., Nucera, S., Scicchitano, M., Bosco, F., Macri, R., Ruga, S., Cardamone, A., Coppoletta, A., Mollace, A., Cognetti, F., and Mollace, V. (2021). Nutraceuticals and cancer: Potential for natural polyphenols. Nutrients, 13(11), 3834.
- Malheiro, R., de Pinho, P. G., Soares, S., da Silva Ferreira, A. C., and Baptista, P. (2013). Volatile biomarkers for wild mushrooms species discrimination. Food Res. Int., 54(1), 186-194.
- Mossman T., (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65:55-3.
- Ohnuma, N., Amemiya, K., Kakuda, R., Yaoita, Y., Machida, K., and Kikuchi, M. (2000) Sterol constituents from two edible mushrooms, Lentinula edodes and Tricholoma matsutake. Chem. Pharm. Bull., 48, 749–751
- Öztürk, C., ve Atila, F. (2021). Mantarların biyolojik aktiviteleri ile ilgili in vitro, in vivo ve klinik değerlendirmeler. J. Fac. Pharmacy of Ankara Uni., 45(2), 344-378.
- Pachon-Pena, G., Reyes-Zurita, F. J., Deffieux, G., Azqueta, A., de Cerain, A.L., Centelles, J.J., Creppy, E.E., and Cascante, M. (2009). Antiproliferative effect of flavomannin-6, 6′-dimethylether from Tricholoma equestre on Caco-2 cells. Toxicol., 264(3), 192-197.
- Sadi, G., Emsen, B., Kaya, A., Kocabaş, A., Çınar, S., and Kartal, D.I. (2015). Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties. Pharmacognosy magazine, 11(Suppl 1), 6.
- Sakai, S., Tomomura, Y., Yoshida, H., Inoue, S., and Kawagishi, H. (2005) Orirubenones D to G, novel phenones from the Tricholoma orirubens mushroom. Biosci. Biotechnol. Biochem., 69, 1630– 1632
- Sesli, E., Asan, A. ve Selçuk, F. (edlr.) Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu.,Yoltaş, A. (2020). Türkiye Mantarları Listesi. İstanbul: Ali Nihat Gökyiğit Vakfı Yayınları.
- Shi, C., Peng, Y. L., He, J., Li, Z. H., Liu, J.K., and Feng, T. (2021). Structures, chemical conversions, and cytotoxicity of tricholopardins C and D, two Tricholoma triterpenoids from the wild mushroom Tricholoma pardinum. Nat. Prod.and Bioprospecting, 11, 235-241.
- Solak, M.H., Işıloğlu, M., Kalmış, E., and Allı H., (2007). Macrofungi of Turkey, checklist .Vol. I, Genç Üniversiteliler Ofset, p: 254, Bornova, İzmir.
- Tel, G., Apaydın, M., Duru, M. E., and Öztürk, M. (2012). Antioxidant and cholinesterase inhibition activities of three Tricholoma species with total phenolic and flavonoid contents: the edible mushrooms from Anatolia. Food Anal. Methods, 5, 495-504.
- Yamaç, M., and Bilgili, F. (2006). Antimicrobial activities of fruit bodies and/or mycelial cultures of some mushroom isolates. Pharm. Biol., 44(9), 660-667.
- Tsukamoto, S., Macabalang, A.D., Nakatani, K., Obara, Y., Nakahata, N., and Ohta, T. (2003) Tricholoma lides A–C, new neurotrophic diterpenes from the mushroom Tricholoma sp. J. Nat. Prod., 66, 1578–1581
- Yoshikawa, K., Kuroboshi, M., Ahagon, S., and Arihara, S. (2004) Three novel crustulinol esters, saponaceols A–C, from Tricholoma saponaceum. Chem. Pharm. Bull., 52, 886–888
- Yılmaz, H.K., ve Özer, R.M. (2022). Ketojenik diyet ve kanser. Haliç Üni. Sağlık Bilimleri Derg., 5(1), 11-19.
- Zhang, A.L., Li, Y.L., Ma, Y.T., Gao, J.M., and Jia, A.Q. (2009) Phenolic meroterpenoids and steroids from the basidiomycete Tricholoma imbricatum. Biochem. Systematics Ecol., 37, 756–758
- Zhou, Y., Li, Y., Zhou, T., Zheng, J., Li, S., and Li, H.B. (2016). Dietary natural products for prevention and treatment of liver cancer. Nutrients, 8(3), 156.
- Wang, H. X., Liu, W. K., Ng, T.B., Ooi, V.E.C., and Chang, S.T. (1995). Immunomodulatory and antitumor activities of a polysaccharide-peptide complex from a mycelial culture of Tricholoma sp., a local edible mushroom. Life Sci., 57(3), 269-281.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Mycology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | April 30, 2024 |
| Published in Issue | Year 2024 Volume: 15 Issue: 1 |
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