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Sıçanlarda Valproik Asite Bağlı Testis Hasarına Rutin Etkisinin Araştırılması

Year 2023, Volume: 16 Issue: 3, 260 - 268, 30.09.2023
https://doi.org/10.30607/kvj.1295945

Abstract

Valproik asit (VALP), epilepsi gibi birçok psikiyatrik hastalık için kullanılan bir ilaçtır. Ancak VALP kullanımının testisler de dahil olmak üzere çeşitli dokularda potansiyel yan etkileri vardır. Rutin (RUT), oksidatif stres kaynaklı hastalıklara ve lipid peroksidasyonuna karşı koruyucu etkileri olan bir flavonoiddir. Bu çalışmada VALP'in neden olduğu testis hasarına karşı RUT'un koruyucu etkisi araştırıldı. Bu amaçla çalışmada ağırlıkları 220-250 g olan 35 adet erkek Sprague-Dawley cinsi rat kullanıldı. Ratlar kontrol (serum fizyolojik), RUT (100 mg/kg/va), VALP, (500mg/kg/va), VALP+RUT 50 (500 mg/kg/va VALP+50 mg/kg/va RUT) ve VALP +RUT 100 (500 mg/kg/v VALP+100 mg/kg/va RUT) olarak 5 gruba ayrıldı. Uygulamalarının sonunda ratlar sakrifiye edilerek testis dokuları biyokimyasal ve spermatolojik analizlerde kullanılmak üzere alındı. Sunulan çalışmanın sonuçlarına göre VALP grubunun testis dokularındaki MDA düzeyi diğer deney gruplarına göre istatistiksel olarak yüksek bulundu (p<0,05). Testis dokusu GSH ve Nrf-2 düzeyi VALP grubunda en düşüktü. Testis dokusunda TNF-α, IL-1β ve MAPK14 seviyeleri VALP grubunda en yüksek iken, RUT tedavi gruplarında azaldı. Benzer şekilde en yüksek Bax, Caspase-3 ve MMP-9 seviyeleri VALP grubunda gözlenirken, RUT tedavisi bu değeri azaltmıştır. Spermatolojik analizler incelendiğinde total motilite değerinin VALP grubunda anlamlı olarak düştüğü, ancak RUT tedavi grubunda total motilite değerinin arttığı görüldü (p<0,05). VALP grubunda ölü ve anormal sperm oranında önemli bir artış bulundu, ancak bu parametreler RUT tedavi grubunda düzeldi. Sonuç olarak RUT, sıçanlarda VALP'nin neden olduğu testis hasarına karşı koruyucu etkilere sahiptir.

References

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Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats

Year 2023, Volume: 16 Issue: 3, 260 - 268, 30.09.2023
https://doi.org/10.30607/kvj.1295945

Abstract

Valproic acid (VALP) is a drug used for many psychiatric diseases such as epilepsy. However, the use of VALP has potential side effects on various tissues, including the testicles. Rutin (RUT) is a flavonoid with protective effects against oxidative stress-induced diseases and lipid peroxidation. In this study, the protective effects of RUT against testicular damage caused by VALP were investigated. For this purpose, 35 male Spraque-Dawley rats weighing 220-250 g were used in the study. The rats were randomly divided into 5 groups as Control (physiological saline), RUT (100 mg/kg/bw), VALP, (500mg/kg/bw), VALP+RUT 50 (500 mg/kg/bw VALP+50 mg/kg/bw RUT), and VALP +RUT 100 (500 mg/kg/bw VALP+100 mg/kg/bw RUT). At the end of the RUT and VALP administrations, the rats were sacrificed and testicular tissues were taken to be used for biochemical and spermatological analyzes. According to the results of this study, the MDA level in the testicular tissues of the VALP group was found to be statistically higher than the other experimental groups (p<0.05). Testicular tissue GSH and Nrf-2 level was the lowest in the VALP group. TNF-α, IL-1Β and MAPK14 levels in testicular tissue were highest in the VALP group, while it was decreased in the RUT treatment groups. Similarly, the highest levels of Bax, Caspase-3 and MMP-9 were observed in the VALP group, while RUT treatment decreased this value. When the spermatological analyzes were examined, it was observed that the total motility value decreased significantly in the VALP group, but the total motility value increased in the RUT treatment group (p<0.05). A significant increase in the rate of dead and abnormal sperm was found in the VALP group, but these parameters improved in the RUT treatment group. As a result, RUT has protective effects against VALP-induced testicular damage in rats.

References

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  • Abarikwu, S. O., Mgbudom-Okah, C. J., & Onuah, C. L. (2022). The protective effect of rutin against busulfan-induced testicular damage in adult rats. Drug and Chemical Toxicology, 45(3), 1035-1043.
  • Abdelkader, N. F., Elyamany, M., Gad, A. M., Assaf, N., Fawzy, H. M., & Elesawy, W. H. (2020). Ellagic acid attenuates liver toxicity induced by valproic acid in rats. Journal of Pharmacological Sciences, 143(1), 23-29.
  • Adewole, K. E., Attah, A. F., & Osawe, S. O. (2021). Exploring phytotherapeutic approach in the management of valproic acid-induced toxicity. Advances in Traditional Medicine, 1-21.
  • Akaras, N., Abuc, O. O., Koc, K., Bal, T., Geyikoglu, F., Atilay, H., Erol, H. S., Yigit, S., & Gul, M. (2020). (1→ 3)‐β‐d‐glucan enhances the toxicity induced by Bortezomib in rat testis. Journal of food biochemistry, 44(3), e13155.
  • Akaras, N., Bal, T., Atilay, H., Selli, J., & Halici, M. (2017). Protective effects of agomelatine on testicular damage caused by bortezomib. Biotechnic & Histochemistry, 92(8), 552-559.
  • Akaras, N., Gur, C., Kucukler, S., & Kandemir, F. M. (2023). Zingerone reduces sodium arsenite-induced nephrotoxicity by regulating oxidative stress, inflammation, apoptosis and histopathological changes. Chemico-Biological Interactions, 374, 110410.
  • Akaras, N., Ileriturk, M., Gur, C., Kucukler, S., Oz, M., & Kandemir, F. M. (2023). The protective effects of chrysin on cadmium-induced pulmonary toxicity; a multi-biomarker approach. Environmental Science and Pollution Research, 1-16.
  • Akondi, B. R., Challa, S. R., & Akula, A. (2011). Protective effects of rutin and naringin in testicular ischemia-reperfusion induced oxidative stress in rats. Journal of reproduction & infertility, 12(3), 209.
  • Aksu, E., Kandemir, F., Özkaraca, M., Ömür, A., Küçükler, S., & Çomaklı, S. (2017). Rutin ameliorates cisplatin‐induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats. Andrologia, 49(1), e12593.
  • Aksu, E. H., Kandemir, F. M., & Küçükler, S. (2021). Ameliorative effect of hesperidin on streptozotocin‐diabetes mellitus‐induced testicular DNA damage and sperm quality degradation in Sprague–Dawley rats. Journal of food biochemistry, 45(10), e13938.
  • Aksu, E. H., Kandemir, F. M., Küçükler, S., & Mahamadu, A. (2018). Improvement in colistin‐induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin. Journal of biochemical and molecular toxicology, 32(11), e22201.
  • Aksu, E. H., Kandemir, F. M., Yıldırım, S., Küçükler, S., Dörtbudak, M. B., Çağlayan, C., & Benzer, F. (2019). Palliative effect of curcumin on doxorubicin‐induced testicular damage in male rats. Journal of biochemical and molecular toxicology, 33(10), e22384.
  • Aktaş, M., Kandemir, F. M., Ozkaraca, M., Hanedan, B., & Kirbaş, A. (2017). Protective effects of rutin on acute lung injury induced by oleic acid in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(3).
  • Alsemeh, A. E., Ahmed, M. M., Fawzy, A., Samy, W., Tharwat, M., & Rezq, S. (2022). Vitamin E rescues valproic acid-induced testicular injury in rats: Role of autophagy. Life Sciences, 296, 120434.
  • Ambriz-Pérez, D. L., Leyva-López, N., Gutierrez-Grijalva, E. P., & Heredia, J. B. (2016). Phenolic compounds: Natural alternative in inflammation treatment. A Review. Cogent Food & Agriculture, 2(1), 1131412.
  • Ansar, S., AlGhosoon, H. T., & Hamed, S. (2015). Evaluation of protective effect of rutin on lead acetate-induced testicular toxicity in Wistar rats. Toxin Reviews, 34(4), 195-199.
  • Bairy, L., Paul, V., & Rao, Y. (2010). Reproductive toxicity of sodium valproate in male rats. Indian journal of pharmacology, 42(2), 90.
  • Belhan, S., Özkaraca, M., Kandemir, F. M., Gülyüz, F., Yildirim, S., ÖMÜR, A. D., & Yener, Z. (2017). Effectiveness of hesperidin on methotrexate-induced testicular toxicity in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(5).
  • Bradbury, C. A., Khanim, F., Hayden, R., Bunce, C., White, D., Drayson, M., Craddock, C., & Turner, B. (2005). Histone deacetylases in acute myeloid leukaemia show a distinctive pattern of expression that changes selectively in response to deacetylase inhibitors. Leukemia, 19(10), 1751-1759.
  • Caglayan, C., Kandemir, F. M., Darendelioğlu, E., Yıldırım, S., Kucukler, S., & Dortbudak, M. B. (2019). Rutin ameliorates mercuric chloride-induced hepatotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. Journal of Trace Elements in Medicine and Biology, 56, 60-68.
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There are 66 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences
Journal Section RESEARCH ARTICLE
Authors

Serkan Ali Akarsu 0000-0003-4450-6540

Nâzım Abdülkadir Kankılıç 0000-0002-3747-3798

Ender Erdoğan 0000-0003-4360-6013

Publication Date September 30, 2023
Acceptance Date July 26, 2023
Published in Issue Year 2023 Volume: 16 Issue: 3

Cite

APA Akarsu, S. A., Kankılıç, N. A., & Erdoğan, E. (2023). Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats. Kocatepe Veterinary Journal, 16(3), 260-268. https://doi.org/10.30607/kvj.1295945
AMA Akarsu SA, Kankılıç NA, Erdoğan E. Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats. kvj. September 2023;16(3):260-268. doi:10.30607/kvj.1295945
Chicago Akarsu, Serkan Ali, Nâzım Abdülkadir Kankılıç, and Ender Erdoğan. “Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats”. Kocatepe Veterinary Journal 16, no. 3 (September 2023): 260-68. https://doi.org/10.30607/kvj.1295945.
EndNote Akarsu SA, Kankılıç NA, Erdoğan E (September 1, 2023) Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats. Kocatepe Veterinary Journal 16 3 260–268.
IEEE S. A. Akarsu, N. A. Kankılıç, and E. Erdoğan, “Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats”, kvj, vol. 16, no. 3, pp. 260–268, 2023, doi: 10.30607/kvj.1295945.
ISNAD Akarsu, Serkan Ali et al. “Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats”. Kocatepe Veterinary Journal 16/3 (September 2023), 260-268. https://doi.org/10.30607/kvj.1295945.
JAMA Akarsu SA, Kankılıç NA, Erdoğan E. Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats. kvj. 2023;16:260–268.
MLA Akarsu, Serkan Ali et al. “Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats”. Kocatepe Veterinary Journal, vol. 16, no. 3, 2023, pp. 260-8, doi:10.30607/kvj.1295945.
Vancouver Akarsu SA, Kankılıç NA, Erdoğan E. Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats. kvj. 2023;16(3):260-8.

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