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EFFECTS OF SOME OF HEAVY METALS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM MILL

Year 2019, Volume: 3 Issue: 4, 371 - 378, 29.10.2019
https://doi.org/10.26900/jsp.3.037

Abstract

In this research, different concentrations of cadmium, nickel, copper
heavy metals applied on 10 weeks old Solanum lycopersicum Mill. cv. invictus
seedlings by irrigation water. As a result of heavy metal applications total
protein amount and peroxidase [EC 1.11.1.7] enzyme activity has been determined
by spectrophotometrically. Changing in total protein amount and peroxidase
activity in S. lycopersicum seedlings after heavy metal applications has been
compared with control group. Depending the application of heavy metals on S.
lycopersicum seedlings total protein amount decreased as 57% in 100 ppm of
copper application and 10,9% increased in 10 ppm nickel application. The
highest increases in peroxidase activity 100 ppm of copper applied and in
proportion as 536,03% and treated group with 10 ppm nickel in proportion as
5.97%were determined.

References

  • BALESTRASSE, K.B., BENAVIDES M.P., GALLEGO S.M., TOMARO M.L., 2003, Effect of Cadmium Stress on Nitrogen Metabolism in Nodules and Roots of Soybean Plants, Functional Plant Biology, 30, 57-64.
  • BAVI, K., KOHELDEBARIN B., MOREDSHAHI A., 2011, Effect of Cadmium On Growth, Protein Content and Peroxidase Activity in Pea Plants, Pak. J. Bot., 43 (3), 1467-1470.
  • BHALERAO, S.A., SHARMA, A.S., POOJARI, A.C., 2015, Toxicity of Nickel in Plants, International Journal Of Pure & Applied Bioscience, 3 (2), 345-355.
  • BRADFORD, M.M., 1976, A Rapid and Sensitive Method for the Quantitation of Microgram Quantites of Protein Utilizing the Principle of Protein-Dye Binding, Analytical Biochemistry, 72, 248-254.
  • CHAVES, L.H.G., ESTRELA, M.A., SOUZA, R.S., 2011, Effect on Plant Growth and Heavy Metal Accumulation by Sunflower, Journal of Phytology, 3 (12), 04-09.
  • ÇÖRDÜK, N., AKINCI, N., KAYA, N., YÜCEL, G., AKI, C., 2016, Effects of Dodine on Total Protein Content and Peroxidase Activity in Vicia faba L., SAÜ Fen Bil Der., 20 (3), 627-633.
  • DALCORSO, G., MANARA, A., PIASENTIN, S., FURINI, A., 2014, Nutrient Metal Elements in Plants, Metallomics, 6 (10), 1770-88.
  • EMAMVERDIAN, A., DING, Y., MOKHBERDORAN, F., XIE, Y., 2015, Heavy Metal Stress and Some Mechanisms of Plant Defense Response, The Scientific World Journal, Volume 2015, 1-18.
  • GEORGIADOU, E.C., KOWALSKA, E., PATLA, K., KULBAT, K., SMOLISNKA, B., LESZCZYNSKA, J., FOTOPOULOS, V., 2018, Influence of Heavy Metals (Ni, Cu, and Zn) on Nitro-Oxidative Stress Responses, Proteome Regulation and Allergen Production in Basil (Ocimum basilicum L.) Plants, Frontiers in Plant Science, 9, 862.
  • JOMOVA, K., and MOROVIC, M., 2009, Effect of Heavy Metal Treatment on Molecular Changes in Root Tips of Lupinus luteus L., Czech J. Food Sci., 27, 386-389.
  • KANNER, J., KINSELLA, J.E., 1983, Lipid Deterioration Initiated by Phagocytic Cells in Muscle Foods: β-Carotene Destruction by a Myeloperoxidase-Hydrogen PeroxideHalide System, Journal of Agricultural and Food Chemistry, 31, 370-376.
  • KISA, D., 2017, The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato, Süleyman Demirel University Journal of Natural and Applied Sciences, 22 (1), 1-6.
  • KİRBAĞ ZENGIN, F., KİRBAĞ, S., 2007, Effects of Copper on Chlorophyll, Proline, Protein and Abscisic Acid Level of Sunflower (Helianthus annuus L.) Seedlings, Journal of Environmental Biology, 28 (3), 561-566.
  • MANAWADU, I.P., NILANTHI D., SENANAYAKE S.G.J.N, 2014, Callus Formation and Organogenesis of Tomato (Lycopersicon esculentum Mill. variety thilina), Tropical Agricultral Research and Extension, 17 (2), 87-94.
  • PERIAGO, M.J., GARCIA-ALONSO, J., JACOB, K., OLIVARES, A.B., BERNAL, M.J., INIESTA, M.D., MARTINEZC., ROS G., 2009, Bioactive Compounds, Folates and Antioxidant Properties of Tomatoes (Lycopersicum esculentum) During Vine Ripening, International Journal of Food Sciences and Nutrition, 60 (8), 694–708.
  • RAI, PK., 2016, Particulate Matter and Its Size Fractionation, Biomagnetic Monitoring of Particulate Matter, Chapter 1, pages 1-13.
  • RAI, P.K., LEES, S., ZHANG, M., TSANG, Y.F., KIM, K.H., 2019, Heavy Metals in Food Crops: Health Risks, Fate, Mechanisms and Management, Environment International, 125, 365–385.
  • RUCINISKA-SOBKOWIAK, R., 2010, Oxidative Stress in Plants Exposed to Heavy Metals, Postepy Biochem., 56 (2),191-200.
  • SHARMA, R.K., AGRAWAL, M. 2005, Biological effects of heavy metals: An overview, Journal of Environmental Biology, 26 (2), 301-313.
  • SINGH, K., PANDEY, S.N., 2011, Effect of Nickel-Stresses on Uptake, Pigments and Antioxidative Responses of Water Lettuce, Pistia stratiotes L. J., Environ. Biol. 32, 391-394.
  • WANG, C., ZHANG, S.H.,WANG, P.F., HOU, J., ZHANG, W.J., LI, W., LIN, Z.P., 2009, The Effect of Excess Zn on Mineral Nutrition and Antioxidative Response in Rapeseed Seedlings., Chemosphere, 75 (11), 1468–1476.

EFFECTS OF SOME OF HEAVY METALS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM MILL

Year 2019, Volume: 3 Issue: 4, 371 - 378, 29.10.2019
https://doi.org/10.26900/jsp.3.037

Abstract

In this research, different concentrations of cadmium, nickel, copper
heavy metals applied on 10 weeks old Solanum lycopersicum Mill. cv. invictus
seedlings by irrigation water. As a result of heavy metal applications total
protein amount and peroxidase [EC 1.11.1.7] enzyme activity has been determined
by spectrophotometrically. Changing in total protein amount and peroxidase
activity in S. lycopersicum seedlings after heavy metal applications has been
compared with control group. Depending the application of heavy metals on S.
lycopersicum seedlings total protein amount decreased as 57% in 100 ppm of
copper application and 10,9% increased in 10 ppm nickel application. The
highest increases in peroxidase activity 100 ppm of copper applied and in
proportion as 536,03% and treated group with 10 ppm nickel in proportion as
5.97%were determined.

References

  • BALESTRASSE, K.B., BENAVIDES M.P., GALLEGO S.M., TOMARO M.L., 2003, Effect of Cadmium Stress on Nitrogen Metabolism in Nodules and Roots of Soybean Plants, Functional Plant Biology, 30, 57-64.
  • BAVI, K., KOHELDEBARIN B., MOREDSHAHI A., 2011, Effect of Cadmium On Growth, Protein Content and Peroxidase Activity in Pea Plants, Pak. J. Bot., 43 (3), 1467-1470.
  • BHALERAO, S.A., SHARMA, A.S., POOJARI, A.C., 2015, Toxicity of Nickel in Plants, International Journal Of Pure & Applied Bioscience, 3 (2), 345-355.
  • BRADFORD, M.M., 1976, A Rapid and Sensitive Method for the Quantitation of Microgram Quantites of Protein Utilizing the Principle of Protein-Dye Binding, Analytical Biochemistry, 72, 248-254.
  • CHAVES, L.H.G., ESTRELA, M.A., SOUZA, R.S., 2011, Effect on Plant Growth and Heavy Metal Accumulation by Sunflower, Journal of Phytology, 3 (12), 04-09.
  • ÇÖRDÜK, N., AKINCI, N., KAYA, N., YÜCEL, G., AKI, C., 2016, Effects of Dodine on Total Protein Content and Peroxidase Activity in Vicia faba L., SAÜ Fen Bil Der., 20 (3), 627-633.
  • DALCORSO, G., MANARA, A., PIASENTIN, S., FURINI, A., 2014, Nutrient Metal Elements in Plants, Metallomics, 6 (10), 1770-88.
  • EMAMVERDIAN, A., DING, Y., MOKHBERDORAN, F., XIE, Y., 2015, Heavy Metal Stress and Some Mechanisms of Plant Defense Response, The Scientific World Journal, Volume 2015, 1-18.
  • GEORGIADOU, E.C., KOWALSKA, E., PATLA, K., KULBAT, K., SMOLISNKA, B., LESZCZYNSKA, J., FOTOPOULOS, V., 2018, Influence of Heavy Metals (Ni, Cu, and Zn) on Nitro-Oxidative Stress Responses, Proteome Regulation and Allergen Production in Basil (Ocimum basilicum L.) Plants, Frontiers in Plant Science, 9, 862.
  • JOMOVA, K., and MOROVIC, M., 2009, Effect of Heavy Metal Treatment on Molecular Changes in Root Tips of Lupinus luteus L., Czech J. Food Sci., 27, 386-389.
  • KANNER, J., KINSELLA, J.E., 1983, Lipid Deterioration Initiated by Phagocytic Cells in Muscle Foods: β-Carotene Destruction by a Myeloperoxidase-Hydrogen PeroxideHalide System, Journal of Agricultural and Food Chemistry, 31, 370-376.
  • KISA, D., 2017, The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato, Süleyman Demirel University Journal of Natural and Applied Sciences, 22 (1), 1-6.
  • KİRBAĞ ZENGIN, F., KİRBAĞ, S., 2007, Effects of Copper on Chlorophyll, Proline, Protein and Abscisic Acid Level of Sunflower (Helianthus annuus L.) Seedlings, Journal of Environmental Biology, 28 (3), 561-566.
  • MANAWADU, I.P., NILANTHI D., SENANAYAKE S.G.J.N, 2014, Callus Formation and Organogenesis of Tomato (Lycopersicon esculentum Mill. variety thilina), Tropical Agricultral Research and Extension, 17 (2), 87-94.
  • PERIAGO, M.J., GARCIA-ALONSO, J., JACOB, K., OLIVARES, A.B., BERNAL, M.J., INIESTA, M.D., MARTINEZC., ROS G., 2009, Bioactive Compounds, Folates and Antioxidant Properties of Tomatoes (Lycopersicum esculentum) During Vine Ripening, International Journal of Food Sciences and Nutrition, 60 (8), 694–708.
  • RAI, PK., 2016, Particulate Matter and Its Size Fractionation, Biomagnetic Monitoring of Particulate Matter, Chapter 1, pages 1-13.
  • RAI, P.K., LEES, S., ZHANG, M., TSANG, Y.F., KIM, K.H., 2019, Heavy Metals in Food Crops: Health Risks, Fate, Mechanisms and Management, Environment International, 125, 365–385.
  • RUCINISKA-SOBKOWIAK, R., 2010, Oxidative Stress in Plants Exposed to Heavy Metals, Postepy Biochem., 56 (2),191-200.
  • SHARMA, R.K., AGRAWAL, M. 2005, Biological effects of heavy metals: An overview, Journal of Environmental Biology, 26 (2), 301-313.
  • SINGH, K., PANDEY, S.N., 2011, Effect of Nickel-Stresses on Uptake, Pigments and Antioxidative Responses of Water Lettuce, Pistia stratiotes L. J., Environ. Biol. 32, 391-394.
  • WANG, C., ZHANG, S.H.,WANG, P.F., HOU, J., ZHANG, W.J., LI, W., LIN, Z.P., 2009, The Effect of Excess Zn on Mineral Nutrition and Antioxidative Response in Rapeseed Seedlings., Chemosphere, 75 (11), 1468–1476.
There are 21 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Natural Sciences
Authors

Gülru Yücel This is me 0000-0001-9785-929X

Cüneyt Akı 0000-0002-7486-2282

Publication Date October 29, 2019
Published in Issue Year 2019 Volume: 3 Issue: 4

Cite

APA Yücel, G., & Akı, C. (2019). EFFECTS OF SOME OF HEAVY METALS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM MILL. Journal of Scientific Perspectives, 3(4), 371-378. https://doi.org/10.26900/jsp.3.037