Deniz Hıyarı (Holothuria tubulosa, Gmelin 1791) Ekstraktlarının Antioksidan ve Antimikrobiyal Aktivitesi
Abstract
Holothuria tubulosa Türkiye denizlerinde bulunan ekonomik ve en çok hasat edilen deniz hıyarı
türlerindendir. Deniz hıyarları insan sağlığı için önemli olan bioaktif bileşiklerin kaynağı olarak bilinmektedir.
Bu çalışmada H. tubulosa’nın antioksidan ve antimikrobiyal etkisinin belirlenmesi amaçlanmıştır. H. tubulosa
ekstraktları, asitontril/trifloroasetik asit, methanol ve su/metanol gibi farklı çözücüler kullanılarak elde edilmiştir.
Sonuçlara göre, ekstraktların antioksidan aktiviteleri, referans antioksidanlara göre tüm konsantrasyonlarda daha
düşük belirlenmiştir. Ancak, yüksek konsantrasyonlarda, ekstraktların antioksidan aktivitesi referans
antioksidanlara yakın derecede bulunmuştur. Bakteriyostatik etki 12 µg/ml konsantrasyonundan sonra
belirlenmiş, ancak bu etki hiçbir konsantrasyon ve ekstrakta, Bacillus cereus için gözlemlenmemiştir. Sonuç
olarak, Türkiye denizlerinde bulunan H. tubulosa’nın, potansiyel doğal antioksidan kaynağı olabileceği
düşünülmektedir.
Keywords
Holothuria tubulosa Deniz Hıyarı Ekstraktı Antimikrobiyal Aktivite Antioksidan Aktivite DPPH
References
- Aleman, A., Santin, E.P., Juchereau, B., Arnaudin, I., Guillen, M.C.G., Montero, P. (2011). Squid gelatin hydrolysates with antihypertensive, anticancer and antioxidant activity. Food Research International, 44, 1044-1051. https://doi.org/10.1016/j.foodres.2011.03.010.
- Althunibat, O.Y., Ridzwan, H., Taher, M., Daud, J.M., Ichwan, S.J.A., Qaralleh, H. (2013). Antioxidant and Cytotoxic Properties of Two Sea Cucumbers, Holothuria edulis Lesson and Stichopus horrens Selenka. Acta Biologica Hungarica, 64(1), 10-20. https://doi.org/10.1556/abiol.64.2013.1.2.
- Aydın, M. (2008). The commercial sea cucumbers fishery in Turkey. SPC Beche de Mer Informaiton Bulletin, 28, 40–43.
- Aydın, M., Sevgili, H., Tufan, B., Emre, Y., Köse, S. (2011). Proximate composition and fatty acid profile of three different fresh and dried commercial sea cucumbers from Turkey. International Jorunal of Food Science and Technology, 46, 500-508. https://doi.org/10.1111/j.1365-2621.2010.02512.x.
- Bougatef, A., Balti, R., Haddar, A., Jellouli, K., Souissi, N., Nasri, M. (2012). Protein hydrolysates from Bluefin Tuna (Thunnus thynnus) heads as influenced by the extent of enzymatic hydrolysis. Biotechnology and Bioprocess Engineering, 17, 841–852. https://doi.org/10.1007/s12257-012-0053-y.
- Çaklı, Ş., Cadun, A., Kişla, D., Dinçer, T. (2004). Determination of quality characteristics of Holothuria tubulosa, (Gmelin, 1788) in Turkish sea (Aegean Region) depending on sun drying process step used in Turkey. Journal of Aquatic Food Product Technology, 13, 69–78. https://doi.org/10.1300/j030v13n03_07.
- Devienne, K.F., Raddi, M.S.G. (2002). Screening for antimicrobial activity of natural products using a microplate photometer. Brazilian Journal of Microbiology, 33, 166-168. https://doi.org/10.1590/s1517-83822002000200014.
- Dobretsov, S., Al-Mammari, I.M., Soussi, B. (2009). Bioactive Compounds from Omani Sea Cucumbers. Agricultural and Marine Sciences, 14, 49-53. https://doi.org/10.24200/jams.vol14iss0pp49-53.
- Esmat, A.Y., Said, M.M., Soliman, A.A., El-Masry, K.S.H., Badiea, E.A. 2013. Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats. Basic nutritional investigation, 29, 258-267. https://doi.org/10.1016/j.nut.2012.06.004.
- Liu, X., Sun, Z., Zhang, M., Meng, X., Xia, X., Yuan, W., Xue, F., Liu, C. (2012). Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus. Carbohydrate Polymers, 90, 1664-1670. https://doi.org/10.1016/j.carbpol.2012.07.047.
- Mashjoor, S., Yousefzadi, M., Pishevarzad, F. (2018). In vitro biological activities of holothurians edible sea cucumbers in the Persian Gulf. Indian Journal of Geo Marine Sciences, 47(07), 1518-1526. http://nopr.niscair.res.in/handle/123456789/44598.
- Molyneux, P. (2004). The use of the stable free radikal diphenylpicryllhydrazly (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26, 211-219.
- Murniasih, T., Putra, M.Y. and Pangestuti, R. (2015). Antioxidant Capacities of Holothuria Sea Cucumbers. Annales Bogorienses, 19, 2. http://dx.doi.org/10.14203/ann.bogor.2015.v19.n2.21-26.
- Nobsathian, S., Tuchinda, P., Sobhon, P., Tinikul, Y., Poljaroen, J., Tinikul, R., Sroyraya, M., Poomton, T., Chaichotranunt, S. (2017). An antioxidant activity of the whole body of Holothuria scabra. Chemical and Biological Technologies in Agriculture, 4, 4. https://doi.org/10.1186/s40538-017-0087-7.
- Öztoprak, B., Doğan, A., Dağlı E. (2014). Checklist of Echinodermata from the coasts of Turkey. Turkish Journal of Zoology, 38, 892-900. https://doi.org/10.3906/zoo-1405-82.
- Peng, X., Xiong, Y.L., Kong, B. (2009). Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chemistry, 113, 196-201. https://doi.org/10.1016/j.foodchem.2008.07.068.
- Qi, H., Li, N., Zhao, X., Xu, Z., Qi, L. (2016). Physicochemical Properties and Radical Scavenging Capacities of Pepsin-Solubilized Collagen from the Body Wall of Starfish (Asterina pectinifera). Journal of Aquatic Food Product Technology, 26(4), 376-389. https://doi.org/10.1080/10498850.2016.1186768.
- Sun, H.H., Mao, W.J., Chen, Y., Guo, S.D., Li, H.Y., Qi, X.H., Chen, Y.L., Xu, J. (2009). Isolation, chemical characteristics and antioxidant properties of the polysaccharides from marine fungus Penicillium sp. F23-2. Carbohydrate Polymers, 78, 117-124. https://doi.org/10.1016/j.carbpol.2009.04.017.
Abstract
Holothuria tubulosa is an economic and most harvested sea cucumber species found in Turkey seas.
Sea cucumbers are known to be sources of some of bioactive components which are play important roles in
human health. In this study, it was aimed to determine the antioxidant and antimicrobial activity of H. tubulosa.
The extracts of H. tubulosa were obtained with using different solvents such as, acetonitrile/trifloroacetic acid,
methanol, and water/methanol. According to results, antioxidant activity of the extracts was lower than the
reference antioxidant agents at all concentrations. However, the activity at higher concentrations of extracts was
found to be closer to reference agents. Bacteriostatic effect was observed after the concentration of 12 µg/ml for
all bacteria except Bacillus cereus, which has also shown resistance to reference antibiotic. As a consequence,
H. tubulosa found in Turkey seas may be regarded as potential source of natural antioxidant agent.
References
- Aleman, A., Santin, E.P., Juchereau, B., Arnaudin, I., Guillen, M.C.G., Montero, P. (2011). Squid gelatin hydrolysates with antihypertensive, anticancer and antioxidant activity. Food Research International, 44, 1044-1051. https://doi.org/10.1016/j.foodres.2011.03.010.
- Althunibat, O.Y., Ridzwan, H., Taher, M., Daud, J.M., Ichwan, S.J.A., Qaralleh, H. (2013). Antioxidant and Cytotoxic Properties of Two Sea Cucumbers, Holothuria edulis Lesson and Stichopus horrens Selenka. Acta Biologica Hungarica, 64(1), 10-20. https://doi.org/10.1556/abiol.64.2013.1.2.
- Aydın, M. (2008). The commercial sea cucumbers fishery in Turkey. SPC Beche de Mer Informaiton Bulletin, 28, 40–43.
- Aydın, M., Sevgili, H., Tufan, B., Emre, Y., Köse, S. (2011). Proximate composition and fatty acid profile of three different fresh and dried commercial sea cucumbers from Turkey. International Jorunal of Food Science and Technology, 46, 500-508. https://doi.org/10.1111/j.1365-2621.2010.02512.x.
- Bougatef, A., Balti, R., Haddar, A., Jellouli, K., Souissi, N., Nasri, M. (2012). Protein hydrolysates from Bluefin Tuna (Thunnus thynnus) heads as influenced by the extent of enzymatic hydrolysis. Biotechnology and Bioprocess Engineering, 17, 841–852. https://doi.org/10.1007/s12257-012-0053-y.
- Çaklı, Ş., Cadun, A., Kişla, D., Dinçer, T. (2004). Determination of quality characteristics of Holothuria tubulosa, (Gmelin, 1788) in Turkish sea (Aegean Region) depending on sun drying process step used in Turkey. Journal of Aquatic Food Product Technology, 13, 69–78. https://doi.org/10.1300/j030v13n03_07.
- Devienne, K.F., Raddi, M.S.G. (2002). Screening for antimicrobial activity of natural products using a microplate photometer. Brazilian Journal of Microbiology, 33, 166-168. https://doi.org/10.1590/s1517-83822002000200014.
- Dobretsov, S., Al-Mammari, I.M., Soussi, B. (2009). Bioactive Compounds from Omani Sea Cucumbers. Agricultural and Marine Sciences, 14, 49-53. https://doi.org/10.24200/jams.vol14iss0pp49-53.
- Esmat, A.Y., Said, M.M., Soliman, A.A., El-Masry, K.S.H., Badiea, E.A. 2013. Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats. Basic nutritional investigation, 29, 258-267. https://doi.org/10.1016/j.nut.2012.06.004.
- Liu, X., Sun, Z., Zhang, M., Meng, X., Xia, X., Yuan, W., Xue, F., Liu, C. (2012). Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus. Carbohydrate Polymers, 90, 1664-1670. https://doi.org/10.1016/j.carbpol.2012.07.047.
- Mashjoor, S., Yousefzadi, M., Pishevarzad, F. (2018). In vitro biological activities of holothurians edible sea cucumbers in the Persian Gulf. Indian Journal of Geo Marine Sciences, 47(07), 1518-1526. http://nopr.niscair.res.in/handle/123456789/44598.
- Molyneux, P. (2004). The use of the stable free radikal diphenylpicryllhydrazly (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26, 211-219.
- Murniasih, T., Putra, M.Y. and Pangestuti, R. (2015). Antioxidant Capacities of Holothuria Sea Cucumbers. Annales Bogorienses, 19, 2. http://dx.doi.org/10.14203/ann.bogor.2015.v19.n2.21-26.
- Nobsathian, S., Tuchinda, P., Sobhon, P., Tinikul, Y., Poljaroen, J., Tinikul, R., Sroyraya, M., Poomton, T., Chaichotranunt, S. (2017). An antioxidant activity of the whole body of Holothuria scabra. Chemical and Biological Technologies in Agriculture, 4, 4. https://doi.org/10.1186/s40538-017-0087-7.
- Öztoprak, B., Doğan, A., Dağlı E. (2014). Checklist of Echinodermata from the coasts of Turkey. Turkish Journal of Zoology, 38, 892-900. https://doi.org/10.3906/zoo-1405-82.
- Peng, X., Xiong, Y.L., Kong, B. (2009). Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chemistry, 113, 196-201. https://doi.org/10.1016/j.foodchem.2008.07.068.
- Qi, H., Li, N., Zhao, X., Xu, Z., Qi, L. (2016). Physicochemical Properties and Radical Scavenging Capacities of Pepsin-Solubilized Collagen from the Body Wall of Starfish (Asterina pectinifera). Journal of Aquatic Food Product Technology, 26(4), 376-389. https://doi.org/10.1080/10498850.2016.1186768.
- Sun, H.H., Mao, W.J., Chen, Y., Guo, S.D., Li, H.Y., Qi, X.H., Chen, Y.L., Xu, J. (2009). Isolation, chemical characteristics and antioxidant properties of the polysaccharides from marine fungus Penicillium sp. F23-2. Carbohydrate Polymers, 78, 117-124. https://doi.org/10.1016/j.carbpol.2009.04.017.
Details
| Primary Language | English |
|---|---|
| Subjects | Hydrobiology |
| Journal Section | Short Communication |
| Authors | |
| Publication Date | December 26, 2018 |
| Submission Date | November 21, 2018 |
| Published in Issue | Year 2018 Volume: 1 Issue: 2 |
