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Rumex crispus Ekstraktı Sıçanlarda Streptozotosin ile Oluşturulan Diyabette Anti-Diyabetik Özellikler Gösterir

Year 2019, Volume: 9 Issue: 4, 1937 - 1943, 01.12.2019
https://doi.org/10.21597/jist.585885

Abstract

Rumex crispus, nehir kenarı ve ıslak çayırlarda kendiliğinden yetişen ve alternatif tıpta yüzyıllardır kullanılan doğal, yabani bir bitkidir. Bu çalışmada, streptozotosin (STZ) aracılığıyla diyabet oluşturulan sıçanlarda R. crispus'un canlı ağırlıkları, açlık kan glukoz seviyeleri, plazma lipid profili, karaciğer enzim aktiviteleri ve antioksidan sisteme etkileri araştırıldı. Hayvanlar 4 gruba ayrıldı. I. grup: kontrol; II. grup: 3 mg kg-1 R. crispus; grup III: 50 mg kg-1 Streptozotosin (STZ) ile diyabet oluşturulan grup; grup IV: 50 mg kg-1 STZ ile diyabet oluşturulan + 3 mg kg-1 R. crispus uygulanan grup şeklinde belirlendi. R. crispus ekstraktının, diyabet oluşumundan 10 gün sonra diyabete bağlı ağırlık kaybını ve kan glikoz seviyesini azalttığı tespit edildi. 14 günlük uygulama süresinin sonunda, R. crispus uygulanan diyabetik sıçanlarda trigliserit, LDL kolesterol (LDL-C) ve VLDL kolesterol (VLDL-C) düzeyleri artarken (P<0.001), HDL kolesterol (HDL-C) seviyeleri anlamlı derecede azaldı (P<0.01). Alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) seviyeleri, R. crispus'a cevaben önemli ölçüde artmıştır (P<0.001). Diyabetik grupta total antioksidan seviye (TAS) anlamlı derecede azalırken, total oksidan seviyenin (TOS) artış gösterdiği belirlendi. Bununla birlikte, R. crispus tedavisine cevap olarak, TAS anlamlı şekilde artmış ve TOS kontrol grubuna göre azalmıştır (her ikisi için de P<0.001). R. crispus ekstraktının takviyesi, sıçanlarda anti-diyabetik özellikler gösterir ve diyabetik bireyler için klinik bir potansiyele sahip olabilir.

References

  • Alezandro MR, Granato D, Genovese MI, 2013. Jaboticaba (Myrciaria jaboticaba (Vell.) Berg), a Brazilian Grape-Like Fruit, Improves Plasma Lipid Profile in Streptozotocin-Mediated Oxidative Stress in Diabetic Rats. Food Research International, 54: 650–659.
  • Aouacheri O, Saka S, Krim M, Messaadia A, Maidi I, 2015. The Investigation of the Oxidative Stress-Related Parameters in Type 2 Diabetes Mellitus. Canadian Journal of Diabetes, 39: 44–49.
  • Atila G, Uslu H, Beytut E, Koç E, Ersan Y, 2015. Anti-Hyperglycaemic, Anti-Oxidant and Anti-Hyperlipidaemic Effects of Momordica charantia L. on Streptozotocin-Induced Diabetic Rats. Indian Journal of Traditional Knowledge, 14: 382–386.
  • Caner C, Özeç AV, Aydın H, Topalkara A, Arıcı MK, Erdoğan H, Toker Mİ, 2012. Comparison of Total Oxidative Stress, Total Antioxidant Capacity, Paraoxonase, Arylesterase, Lipid Peroxidase Levels in Humor Aquos and Serum at Diabetic and Nondiabetic Patients with Cataract. Turkish Journal of Ophthalmology, 42: 47–52.
  • Coruh I, Gormez A, Ercisli S, Sengul M, 2008. Total Phenolic Content, Antioxidant, and Antibacterial Activity of Rumex crispus Grown Wild in Turkey. Pharmaceutical Biology, 46: 634–638.
  • Degirmenci I, Ustuner MC, Kalender Y, Kalender S, Gunes HV, 2005. The Effects of Acarbose and Rumex patientia L. on Ultrastructural and Biochemical Changes of Pancreatic B Cells in Streptozotocin-Induced Diabetic Rats. Journal of Ethnopharmacology, 97: 555–559.
  • Elzaawely AA, Tawata S, 2012. Antioxidant Capacity and Phenolic Content of Rumex dentatus L. Grown in Egypt. Journal of Crop Science and Biotechnology, 15: 59–64.
  • Ha BG, Yonezawa T, Son MJ, Woo JT, Ohba S, Chung U-I, Yagasaki K, 2014. Antidiabetic Effect of Nepodin, A Component of Rumex Roots, and its Modes of Action in Vitro and In vivo. BioFactors, 40: 436–447.
  • Hamdan II, Afifi FU, 2004. Studies on the In vitro and In vivo Hypoglycemic Activities of Some Medicinal Plants Used in Treatment of Diabetes in Jordanian Traditional Medicine. Journal of Ethnopharmacology, 93: 117–121.
  • Kim HJ, Jung CL, Park IS, Suh H-J, Kwon OO, Kim J-S, 2013. Nrf2-Mediated Induction of Phase 2 Detoxifying Enzymes by Curled dock (Rumex crispus L.) Seed Extract. Food Science and Biotechnology, 22: 795–802.
  • Lupi R, Del Guerra S, Mancarella R, Novelli M, Valgimigli L, Pedulli GF, Paolini M, Soleti A, Filipponi F, Mosca F, 2007. Insulin Secretion Defects of Human Type 2 Diabetic Islets are Corrected In vitro by A New Reactive Oxygen Species Scavenger. Diabetes & Metabolism, 33: 340–345.
  • Maksimović Z, Kovačević N, Lakušić B, Ćebović T, 2011. Antioxidant Activity of Yellow dock (Rumex crispus L., Polygonaceae) Fruit Extract. Phytotherapy Research, 25: 101–105.
  • Mayer J, Donnelly TM, 2013. Clinical veterinary advisor, birds and exotic pets, 1: Clinical Veterinary Advisor. Elsevier Health Sciences.
  • Muselin F, Brezovan D, Savici J, Cristina RT, Dumitrescu E, Doma AO, Morar D, Trif A, 2015. The Use of Yellow dock (Rumex crispus L.) and Goji berry (Lycium barbarum L.) in Alloxan Induced Diabetes Mellitus in Rats. Scientific Papers Animal Science and Biotechnologies, 48: 373–376.
  • Nawale RB, Mourya VK, Bhise SB, 2006. Non-Enzymatic Glycation of Proteins: A Cause for Complications in Diabetes. Indian Journal Of Biochemistry & Biophysics, 43: 337–344.
  • Nogueira-Machado JA, e Silva FL, Cunha EP, Calsolari MR, Costa DC, Perilo CS, Horta BC, Ferreira IC, Chaves MM, 2006. Modulation of the Production of Reactive Oxygen Species (ROS) by cAMP-Elevating Agents in Granulocytes From Diabetic Patients: An Akt/PKB-Dependent Phenomenon. Diabetes & metabolism, 32: 331–335.
  • Özgen U, Kaya Y, Houghton P, 2012. Folk Medicines in the Villages of Ilıca District (Erzurum, Turkey). Turkish Journal of Biology, 36: 93–106.
  • Özkan O, Sarı B, Bayezit M, Doğan A, Akpulat HA, Erdağ D, 2010. Anti-Coccidiosis Effect of Thymus serpyllum in Rabbits: Oocyst Shedding and Body Weight Changes. Journal of the Faculty of Veterinary Medicine of Kafkas University, 16: 323–327.
  • Sedaghat R, Roghani M, Ahmadi M, Ahmadi F, 2011. Antihyperglycemic and Antihyperlipidemic Effect of Rumex patientia Seed Preparation in Streptozotocin-Diabetic Rats. Pathophysiology, 18: 111–115.
  • Shiwani S, Singh NK, Wang MH, 2012. Carbohydrase Inhibition and Anti-Cancerous and Free Radical Scavenging Properties Along With DNA and Protein Protection Ability of Methanolic Root Extracts of Rumex crispus. Nutrition Research And Practice, 6: 389–395.
  • Uslu H, Uslu Atila G, Özen H, Karaman M, 2018. Effects of Different Doses of Prunus laurocerasus L. Leaf Extract on Oxidative Stress, Hyperglycaemia and Hyperlipidaemia Induced by Type I Diabetes. Indian Journal of Traditional Knowledge, 17: 430–436.
  • Xavier S, Sadanandan J, George N, Paulose CS, 2012. β2-Adrenoceptor and Insulin Receptor Expression in the Skeletal Muscle of Streptozotocin Induced Diabetic Rats: Antagonism by Vitamin D3 And Curcumin. European Journal of Pharmacology, 687: 14–20.
  • Yaman T, Doğan A, 2016. Protective Effects of Acorn (Quercus Branti lindl.) Extract on Liver And Pancreas in Streptozotocin-Induced Diabetic Rats. The Journal of Faculty of Veterinary Medicine of Dicle University, 1: 7–15.
  • Zareei S, Boojar MM, Amanlou M, 2017. Inhibition of Liver Alanine Aminotransferase and Aspartate Aminotransferase by Hesperidin and its Aglycone Hesperetin: An In vitro and In silico study. Life Sciences, 178: 49–55.

Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats

Year 2019, Volume: 9 Issue: 4, 1937 - 1943, 01.12.2019
https://doi.org/10.21597/jist.585885

Abstract

Rumex crispus is a natural, wild plant that grows spontaneously on riverside and wet meadows, and has been used for centuries in alternative medicine. In the present study, the effects of R. crispus on body weights, fasting blood glucose levels, plasma lipid profile, liver enzyme activities and antioxidant system were investigated in streptozotocin (STZ) induced diabetic rats. The animals were divided into 4 groups. Group I: control; group II: 3 mg kg-1 R. crispus; group III: 50 mg kg-1 Streptozotocin (STZ)-induced diabetes; group IV: 50 mg kg-1 STZ-induced diabetes + 3 mg kg-1 R.crispus. It was found that R. crispus extract reduced diabetes-related weight loss and blood glucose levels 10 days after the formation of diabetes. At the end of 14-day treatment period, HDL cholesterol (HDL-C) levels significantly decreased (P<0.01) while triglyceride, LDL cholesterol (LDL-C) and VLDL cholesterol (VLDL-C) levels increased in R. crispus supplemented diabetic rats (P<0.001). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly increased in response to R. crispus (P <0.001). Total antioxidant status (TAS) significantly decreased and total oxidant status (TOS) increased in diabetic group; however, in response to R. crispus treatment, TAS significantly increased and TOS decreased relative to control group (P<0.001, for both). Supplementation of R. crispus extract shows anti-diabetic properties in rats and might have a clinical potential for diabetic individuals.

References

  • Alezandro MR, Granato D, Genovese MI, 2013. Jaboticaba (Myrciaria jaboticaba (Vell.) Berg), a Brazilian Grape-Like Fruit, Improves Plasma Lipid Profile in Streptozotocin-Mediated Oxidative Stress in Diabetic Rats. Food Research International, 54: 650–659.
  • Aouacheri O, Saka S, Krim M, Messaadia A, Maidi I, 2015. The Investigation of the Oxidative Stress-Related Parameters in Type 2 Diabetes Mellitus. Canadian Journal of Diabetes, 39: 44–49.
  • Atila G, Uslu H, Beytut E, Koç E, Ersan Y, 2015. Anti-Hyperglycaemic, Anti-Oxidant and Anti-Hyperlipidaemic Effects of Momordica charantia L. on Streptozotocin-Induced Diabetic Rats. Indian Journal of Traditional Knowledge, 14: 382–386.
  • Caner C, Özeç AV, Aydın H, Topalkara A, Arıcı MK, Erdoğan H, Toker Mİ, 2012. Comparison of Total Oxidative Stress, Total Antioxidant Capacity, Paraoxonase, Arylesterase, Lipid Peroxidase Levels in Humor Aquos and Serum at Diabetic and Nondiabetic Patients with Cataract. Turkish Journal of Ophthalmology, 42: 47–52.
  • Coruh I, Gormez A, Ercisli S, Sengul M, 2008. Total Phenolic Content, Antioxidant, and Antibacterial Activity of Rumex crispus Grown Wild in Turkey. Pharmaceutical Biology, 46: 634–638.
  • Degirmenci I, Ustuner MC, Kalender Y, Kalender S, Gunes HV, 2005. The Effects of Acarbose and Rumex patientia L. on Ultrastructural and Biochemical Changes of Pancreatic B Cells in Streptozotocin-Induced Diabetic Rats. Journal of Ethnopharmacology, 97: 555–559.
  • Elzaawely AA, Tawata S, 2012. Antioxidant Capacity and Phenolic Content of Rumex dentatus L. Grown in Egypt. Journal of Crop Science and Biotechnology, 15: 59–64.
  • Ha BG, Yonezawa T, Son MJ, Woo JT, Ohba S, Chung U-I, Yagasaki K, 2014. Antidiabetic Effect of Nepodin, A Component of Rumex Roots, and its Modes of Action in Vitro and In vivo. BioFactors, 40: 436–447.
  • Hamdan II, Afifi FU, 2004. Studies on the In vitro and In vivo Hypoglycemic Activities of Some Medicinal Plants Used in Treatment of Diabetes in Jordanian Traditional Medicine. Journal of Ethnopharmacology, 93: 117–121.
  • Kim HJ, Jung CL, Park IS, Suh H-J, Kwon OO, Kim J-S, 2013. Nrf2-Mediated Induction of Phase 2 Detoxifying Enzymes by Curled dock (Rumex crispus L.) Seed Extract. Food Science and Biotechnology, 22: 795–802.
  • Lupi R, Del Guerra S, Mancarella R, Novelli M, Valgimigli L, Pedulli GF, Paolini M, Soleti A, Filipponi F, Mosca F, 2007. Insulin Secretion Defects of Human Type 2 Diabetic Islets are Corrected In vitro by A New Reactive Oxygen Species Scavenger. Diabetes & Metabolism, 33: 340–345.
  • Maksimović Z, Kovačević N, Lakušić B, Ćebović T, 2011. Antioxidant Activity of Yellow dock (Rumex crispus L., Polygonaceae) Fruit Extract. Phytotherapy Research, 25: 101–105.
  • Mayer J, Donnelly TM, 2013. Clinical veterinary advisor, birds and exotic pets, 1: Clinical Veterinary Advisor. Elsevier Health Sciences.
  • Muselin F, Brezovan D, Savici J, Cristina RT, Dumitrescu E, Doma AO, Morar D, Trif A, 2015. The Use of Yellow dock (Rumex crispus L.) and Goji berry (Lycium barbarum L.) in Alloxan Induced Diabetes Mellitus in Rats. Scientific Papers Animal Science and Biotechnologies, 48: 373–376.
  • Nawale RB, Mourya VK, Bhise SB, 2006. Non-Enzymatic Glycation of Proteins: A Cause for Complications in Diabetes. Indian Journal Of Biochemistry & Biophysics, 43: 337–344.
  • Nogueira-Machado JA, e Silva FL, Cunha EP, Calsolari MR, Costa DC, Perilo CS, Horta BC, Ferreira IC, Chaves MM, 2006. Modulation of the Production of Reactive Oxygen Species (ROS) by cAMP-Elevating Agents in Granulocytes From Diabetic Patients: An Akt/PKB-Dependent Phenomenon. Diabetes & metabolism, 32: 331–335.
  • Özgen U, Kaya Y, Houghton P, 2012. Folk Medicines in the Villages of Ilıca District (Erzurum, Turkey). Turkish Journal of Biology, 36: 93–106.
  • Özkan O, Sarı B, Bayezit M, Doğan A, Akpulat HA, Erdağ D, 2010. Anti-Coccidiosis Effect of Thymus serpyllum in Rabbits: Oocyst Shedding and Body Weight Changes. Journal of the Faculty of Veterinary Medicine of Kafkas University, 16: 323–327.
  • Sedaghat R, Roghani M, Ahmadi M, Ahmadi F, 2011. Antihyperglycemic and Antihyperlipidemic Effect of Rumex patientia Seed Preparation in Streptozotocin-Diabetic Rats. Pathophysiology, 18: 111–115.
  • Shiwani S, Singh NK, Wang MH, 2012. Carbohydrase Inhibition and Anti-Cancerous and Free Radical Scavenging Properties Along With DNA and Protein Protection Ability of Methanolic Root Extracts of Rumex crispus. Nutrition Research And Practice, 6: 389–395.
  • Uslu H, Uslu Atila G, Özen H, Karaman M, 2018. Effects of Different Doses of Prunus laurocerasus L. Leaf Extract on Oxidative Stress, Hyperglycaemia and Hyperlipidaemia Induced by Type I Diabetes. Indian Journal of Traditional Knowledge, 17: 430–436.
  • Xavier S, Sadanandan J, George N, Paulose CS, 2012. β2-Adrenoceptor and Insulin Receptor Expression in the Skeletal Muscle of Streptozotocin Induced Diabetic Rats: Antagonism by Vitamin D3 And Curcumin. European Journal of Pharmacology, 687: 14–20.
  • Yaman T, Doğan A, 2016. Protective Effects of Acorn (Quercus Branti lindl.) Extract on Liver And Pancreas in Streptozotocin-Induced Diabetic Rats. The Journal of Faculty of Veterinary Medicine of Dicle University, 1: 7–15.
  • Zareei S, Boojar MM, Amanlou M, 2017. Inhibition of Liver Alanine Aminotransferase and Aspartate Aminotransferase by Hesperidin and its Aglycone Hesperetin: An In vitro and In silico study. Life Sciences, 178: 49–55.
There are 24 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Biyoloji / Biology
Authors

Özgen Çelik This is me 0000-0001-7819-5598

Evren Koç 0000-0002-0022-9433

Publication Date December 1, 2019
Submission Date July 2, 2019
Acceptance Date July 23, 2019
Published in Issue Year 2019 Volume: 9 Issue: 4

Cite

APA Çelik, Ö., & Koç, E. (2019). Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats. Journal of the Institute of Science and Technology, 9(4), 1937-1943. https://doi.org/10.21597/jist.585885
AMA Çelik Ö, Koç E. Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats. J. Inst. Sci. and Tech. December 2019;9(4):1937-1943. doi:10.21597/jist.585885
Chicago Çelik, Özgen, and Evren Koç. “Rumex Crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats”. Journal of the Institute of Science and Technology 9, no. 4 (December 2019): 1937-43. https://doi.org/10.21597/jist.585885.
EndNote Çelik Ö, Koç E (December 1, 2019) Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats. Journal of the Institute of Science and Technology 9 4 1937–1943.
IEEE Ö. Çelik and E. Koç, “Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats”, J. Inst. Sci. and Tech., vol. 9, no. 4, pp. 1937–1943, 2019, doi: 10.21597/jist.585885.
ISNAD Çelik, Özgen - Koç, Evren. “Rumex Crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats”. Journal of the Institute of Science and Technology 9/4 (December 2019), 1937-1943. https://doi.org/10.21597/jist.585885.
JAMA Çelik Ö, Koç E. Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats. J. Inst. Sci. and Tech. 2019;9:1937–1943.
MLA Çelik, Özgen and Evren Koç. “Rumex Crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats”. Journal of the Institute of Science and Technology, vol. 9, no. 4, 2019, pp. 1937-43, doi:10.21597/jist.585885.
Vancouver Çelik Ö, Koç E. Rumex crispus Extract Exerts Anti-Diabetic Properties in Streptozotocin-Induced Diabetes in Rats. J. Inst. Sci. and Tech. 2019;9(4):1937-43.