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Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes

Year 2023, Volume: 9 Issue: 4, 923 - 930, 22.12.2023

Namuk Ergün Güray Akdogan Saime İkincikarakaya

https://doi.org/10.28979/jarnas.1286891

Abstract

The consumption of naked barley is significantly increasing as more people become aware of its benefi-cial role as a source of dietary fiber and β-glucan. As a result, breeding programs paid more attention to naked barley. Improvement of yield and quality of naked barley is hindered by the lack of available germplasm. Mutation breeding is an effective tool for generating variation for plant breeding. Chlorophyll mutations are often used as visual indicators in breeding research to determine the optimum mutagen dosage. The purpose of this study was to identify the types and frequency of chlorophyll mutations brought on by different gamma radiation doses in two genotypes of hulless barley and determine the effective dose (ED50) based on the mutation frequency. Seeds of naked barley line YAA7050-14 and cv. Yalin that have been irradiated with doses of 100, 150, 200, and 300 Gy gamma rays delivered by a Cobalt-60 source. Chlorophyll mutations were observed in 8-day-old M2 plants grown under greenhouse conditions. In the M2 plants of cv. Yalin, the highest mutagen frequency was observed at 250 and 300 Gy, while in line YAA7050-14, the highest mutation frequency was found at 300 Gy. The rate of chlorophyll mutation rose in both genotypes as the gamma ray doses increased. The albino type of chlorophyll muta-tion was found in the cv. Yalin at the greatest rate, whereas the xantha type was found in the line YAA7050-14. The most common chlorophyll mutation type was albino, while the least common type was viridis when both genotypes were considered together. Based on the mutation frequency, 250-300 Gy doses could be used to effectively in further research to create mutations in the naked barley genotypes.

Keywords

Chlorophyll mutations gamma irradiation M2 plants naked barley

Thanks

The authors are grateful to Dr Ali Senay from the Ankara Nuclear Research and Training Centre for his help with gamma irradiation. We are also grateful to CRIFC for allowing us to use their greenhouse. This study is based on Namuk ERGÜN's PhD thesis completed at Ankara University, Graduate Scholl of Natural and Applied Sciences under the supervision of Saime ÜNVER İKİNCİKARAKAYA.

References

  • Ahumada-Flores, S., Briceño-Zamora, M., García-Montoya, J., López-Cázarez, C., Pereo-Galvez, A., Parra-Cota, F. & de los Santos-Villalobos, S. (2020). Gamma radiosensitivity study on wheat (Triticum turgidum ssp. durum). Open Agriculture, 5(1), 558-562. https://doi.org/10.1515/opag-2020-0057
  • Ahumada-Flores, S., Pando, L. R. G., Cota, F. I. P., de la Cruz Torres, E., Sarsu, F., & de los Santos Villalobos, S. (2021). Gamma irradiation induces changes of phenotypic and agronomic traits in wheat (Triticum turgidum ssp. durum). Applied Radiation and Isotopes, 167, 109490. https://doi.org/10.1016/j.apradiso.2020.109490
  • Arain, A. G. (1974). The effect of ethyl methanesulfonate treatment on floret sterility and chlorophyll mutation rate in barley. Radiation Botany, 14(4), 347-350. https://doi.org/10.1016/S0033-7560(74)80027-X
  • Badr, A., Rabey, H. E., Effgen, S., Ibrahim, H. H., Pozzi, C., Rohde, W., & Salamini, F. (2000). On the origin and domestication history of barley (Hordeum vulgare). Molecular biology and evolution, 17(4), 499-510. https://doi.org/10.1093/oxfordjournals.molbev.a026330
  • Bhatty, R. S. (1999). The potential of hull‐less barley. Cereal Chemistry, 76(5), 589-599. https://doi.org/10.1094/CCHEM.1999.76.5.589
  • Chaudhary, J., Deshmukh, R., & Sonah, H. (2019). Mutagenesis Approaches and Their Role in Crop Improvement. Plants (Basel, Switzerland), 8(11), 467. https://doi.org/10.3390/plants8110467
  • Çiftçi C.Y. & Şenay, A. (2005) Effect of seperate and combined treatments of different doses of gamma rays and EMS on durum wheat (Tritium durum Desf.) in M1 generations. Journal of Field Crops Central Research Institute 14(1-2), 41-49. (in Turkish) https://www.biotechstudies.org/uploads/pdf_329.pdf
  • Dyulgerova, B., & Dyulgerov, N. (2022). Mutagenic effect of sodium azide on winter barley cultivars. Agriıcultural Science and Technology, 14(2), 27-33. https://doi: 10.15547/ast.2022.02.016
  • Gaul, H. (1964). Mutations in plant breeding. Radiation Botany, 4(3), 155–232. https://doi:10.1016/s0033-7560(64)80069-7
  • Goyal, S., Wani, M.R. & Khan, S. (2019) Frequency and Spectrum of Chlorophyll Mutations Induced by Single and Combination Treatments of Gamma Rays and EMS in Urdbean. Asian Journal of Biological Sciences, 12, 156-163. https://scialert.net/abstract/?doi=ajbs.2019.156.163
  • Gustafsson, Å. (1940). The Mutation system of the chlorophyll apparatus. Acta Universitatis Lunden-sis, 36(11), 1-40.
  • Gustafsson, Å. (1946). The origin of albina and xantha mutations in barley. Acta radiologica, 27(3-4), 300–307. https://doi.org/10.3109/00016924609135187
  • Ilhan, D. (2014) Mutagenicity tests used in plants. Kafkas University Institute of Natural and Applied Science Journal, 7(1), 29-47. (in Turkish) https://dergipark.org.tr/en/pub/kujs/issue/30901/334479
  • Kacar, B. (1996). Plant Physiology 4th Edition, Ankara University Faculty of Agriculture Publications, Publication No: 1447, 424, Ankara (in Turkish).
  • Kodym A., Afza, R., Forster, B.P., Ukai, Y & Nakagawa, H. (2012) Methodology for physical and chemical mutagenic treatments. In: Shu Q.Y., Forster, B.F. & Nakagawa, H. (eds), Plant muta-tion breeding and biotechnology. CABI, FAO, Oxfordshire, UK pp. 169–180
  • Lev-Yadun, S., Gopher, A., & Abbo, S. (2000). The cradle of agriculture. Science, 288(5471), 1602-1603. https://doi.org/10.1126/science.288.5471.1602
  • Liu, Z. L., Yuan, S., Liu, W. J., Du, J. B., Tian, W. J., Luo, M. H., & Lin, H. H. (2008). Mutation mechanism of chlorophyll-less barley mutant NYB. Photosynthetica, 46, 73-78. https://doi.org/10.1007/s11099-008-0013-0
  • Makowska, K. & Oleszczuk, S. (2013) Albinism in barley androgenesis. Plant Cell Reports, 33, 385-392. https://doi.org/10.1007/s00299-013-1543-x
  • Maluszynski, M., Szarejko, I., Bhatia, R., Nichterlein, K. & Lagoda, P.J.L. (2009) Mutation techniques. In: Guimares, E., Ceccarelli, S., Weltzein, E. & Rajendran, P.G. (eds) Plant breeding book. FAO, Rome, pp 159-194.
  • Marcu, D., Damian, G., Cosma, C., & Cristea, V. (2013). Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays). Journal of biological physics, 39, 625-634. https://doi.org/10.1007/s10867-013-9322-z
  • Meints, B., Vallejos, C., & Hayes, P. (2021). Multi-use naked barley: A new frontier. Journal of Ce-real Science, 102, 103370. https://doi.org/10.1016/j.jcs.2021.103370
  • Meints, B. & Hayes, P.M. (2019) Breeding naked barley for food, feed, and malt. In: Goldman, I. (ed) Plant Breeding Reviews, 43 (1). John Wiley & Sons, Inc., pp. 95-119.
  • Motoyoshi, F. (1967). Chlorophyll formation in several chlorophyll mutants of sand oats and barley cultured on nutrient media. The Japanese Journal of Genetics, 42(5), 291-297. https://doi.org/10.1266/jjg.42.291
  • Nazarenko, M. M., & Lykholat, T. Y. (2020). Variability at winter wheat varieties first generation which obtained mutagen action. Ecology and Noospherology, 31(2), 77-81. https://doi.org/10.15421/032012 Newman, R.K. & Newman, C.W. (2008) Barley for food and health: Science, technology, and products. John Wiley & Sons, Inc., 243, New Jersey.
  • Patial, M., Thakur, S. R., Singh, K. P., & Thakur, A. (2017). Frequency and spectrum of chlorophyll mutations and induced variability in ricebean (Vigna umbellata Thunb, Ohwi and Ohashi). Legume Research-An International Journal, 40(1), 39-46. https://doi.org/10.18805/lr.v0iOF. 10757
  • Sakin, M.A. & Sencar, Ö. (2001). Makarnalık buğday (Triticum durum Desf.) çeşitlerinin mutagenlere tepkileri. Journal of Agricultural Faculty of Gaziosmanpaşa University, 18(1), 89-93. https://dergipark.org.tr/tr/pub/gopzfd/issue/7349/96160 (in Turkish)
  • Simpson, D. J., Machold, O., Høyer-Hansen, G., & Von Wettstein, D. (1985). Chlorina mutants of barley (Hordeum vulgare L.). Carlsberg Research Communications, 50, 223-238. https://doi.org/10.1007/BF02907148 Singh, R. M., Singh, J., & Srivastava, A. N. (1977). Mutagenic effects of gamma rays, EMS, and HA in barley. Barley Genetics Newsletter, 7, 60.
  • Ullrich, S.E. (2011). Significance, adaptation, production, and trade of barley. In: Ullrich, S.E. (ed.). Barley, Blackwell Publishing Ltd., , Chichester, UK. pp. 3-13
  • Ünver, S. (1989). The effect of different doses of EMS and the temperatures and periods of post washing applied on the some characters in M1 and M2 plants of barley (Hordeum vulgare L.) (in Turkish) (Unpublished PhD thesis). Ankara University, Ankara, Turkey.
  • Wani, M. R., Khan, S., & Kozgar, M. I. (2011). Induced chlorophyll mutations. I. Mutagenic effectiveness and efficiency of EMS, HZ and SA in mungbean. Frontiers of Agriculture in China, 5, 514-518. https://doi.org/10.1007/s11703-011-1126-y
  • Wani, M.R. 2017. Induced chlorophyll mutations, comparative mutagenic effectiveness and efficiency of chemical mutagens in lentils (Lens culinaris Medik). Asian J. Plant Sci., 16: 221-226. https://doi.org/10.3923/ajps.2017.221.226
  • Yu, K., Lenz-Wiedemann, V., Chen, X., & Bareth, G. (2014). Estimating leaf chlorophyll of barley at different growth stages using spectral indices to reduce soil background and canopy structure effects. ISPRS Journal of Photogrammetry and Remote Sensing, 97, 58-77. https://doi.org/10.1016/j.isprsjprs.2014.08.005

Year 2023, Volume: 9 Issue: 4, 923 - 930, 22.12.2023

Namuk Ergün Güray Akdogan Saime İkincikarakaya

https://doi.org/10.28979/jarnas.1286891

Abstract

References

  • Ahumada-Flores, S., Briceño-Zamora, M., García-Montoya, J., López-Cázarez, C., Pereo-Galvez, A., Parra-Cota, F. & de los Santos-Villalobos, S. (2020). Gamma radiosensitivity study on wheat (Triticum turgidum ssp. durum). Open Agriculture, 5(1), 558-562. https://doi.org/10.1515/opag-2020-0057
  • Ahumada-Flores, S., Pando, L. R. G., Cota, F. I. P., de la Cruz Torres, E., Sarsu, F., & de los Santos Villalobos, S. (2021). Gamma irradiation induces changes of phenotypic and agronomic traits in wheat (Triticum turgidum ssp. durum). Applied Radiation and Isotopes, 167, 109490. https://doi.org/10.1016/j.apradiso.2020.109490
  • Arain, A. G. (1974). The effect of ethyl methanesulfonate treatment on floret sterility and chlorophyll mutation rate in barley. Radiation Botany, 14(4), 347-350. https://doi.org/10.1016/S0033-7560(74)80027-X
  • Badr, A., Rabey, H. E., Effgen, S., Ibrahim, H. H., Pozzi, C., Rohde, W., & Salamini, F. (2000). On the origin and domestication history of barley (Hordeum vulgare). Molecular biology and evolution, 17(4), 499-510. https://doi.org/10.1093/oxfordjournals.molbev.a026330
  • Bhatty, R. S. (1999). The potential of hull‐less barley. Cereal Chemistry, 76(5), 589-599. https://doi.org/10.1094/CCHEM.1999.76.5.589
  • Chaudhary, J., Deshmukh, R., & Sonah, H. (2019). Mutagenesis Approaches and Their Role in Crop Improvement. Plants (Basel, Switzerland), 8(11), 467. https://doi.org/10.3390/plants8110467
  • Çiftçi C.Y. & Şenay, A. (2005) Effect of seperate and combined treatments of different doses of gamma rays and EMS on durum wheat (Tritium durum Desf.) in M1 generations. Journal of Field Crops Central Research Institute 14(1-2), 41-49. (in Turkish) https://www.biotechstudies.org/uploads/pdf_329.pdf
  • Dyulgerova, B., & Dyulgerov, N. (2022). Mutagenic effect of sodium azide on winter barley cultivars. Agriıcultural Science and Technology, 14(2), 27-33. https://doi: 10.15547/ast.2022.02.016
  • Gaul, H. (1964). Mutations in plant breeding. Radiation Botany, 4(3), 155–232. https://doi:10.1016/s0033-7560(64)80069-7
  • Goyal, S., Wani, M.R. & Khan, S. (2019) Frequency and Spectrum of Chlorophyll Mutations Induced by Single and Combination Treatments of Gamma Rays and EMS in Urdbean. Asian Journal of Biological Sciences, 12, 156-163. https://scialert.net/abstract/?doi=ajbs.2019.156.163
  • Gustafsson, Å. (1940). The Mutation system of the chlorophyll apparatus. Acta Universitatis Lunden-sis, 36(11), 1-40.
  • Gustafsson, Å. (1946). The origin of albina and xantha mutations in barley. Acta radiologica, 27(3-4), 300–307. https://doi.org/10.3109/00016924609135187
  • Ilhan, D. (2014) Mutagenicity tests used in plants. Kafkas University Institute of Natural and Applied Science Journal, 7(1), 29-47. (in Turkish) https://dergipark.org.tr/en/pub/kujs/issue/30901/334479
  • Kacar, B. (1996). Plant Physiology 4th Edition, Ankara University Faculty of Agriculture Publications, Publication No: 1447, 424, Ankara (in Turkish).
  • Kodym A., Afza, R., Forster, B.P., Ukai, Y & Nakagawa, H. (2012) Methodology for physical and chemical mutagenic treatments. In: Shu Q.Y., Forster, B.F. & Nakagawa, H. (eds), Plant muta-tion breeding and biotechnology. CABI, FAO, Oxfordshire, UK pp. 169–180
  • Lev-Yadun, S., Gopher, A., & Abbo, S. (2000). The cradle of agriculture. Science, 288(5471), 1602-1603. https://doi.org/10.1126/science.288.5471.1602
  • Liu, Z. L., Yuan, S., Liu, W. J., Du, J. B., Tian, W. J., Luo, M. H., & Lin, H. H. (2008). Mutation mechanism of chlorophyll-less barley mutant NYB. Photosynthetica, 46, 73-78. https://doi.org/10.1007/s11099-008-0013-0
  • Makowska, K. & Oleszczuk, S. (2013) Albinism in barley androgenesis. Plant Cell Reports, 33, 385-392. https://doi.org/10.1007/s00299-013-1543-x
  • Maluszynski, M., Szarejko, I., Bhatia, R., Nichterlein, K. & Lagoda, P.J.L. (2009) Mutation techniques. In: Guimares, E., Ceccarelli, S., Weltzein, E. & Rajendran, P.G. (eds) Plant breeding book. FAO, Rome, pp 159-194.
  • Marcu, D., Damian, G., Cosma, C., & Cristea, V. (2013). Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays). Journal of biological physics, 39, 625-634. https://doi.org/10.1007/s10867-013-9322-z
  • Meints, B., Vallejos, C., & Hayes, P. (2021). Multi-use naked barley: A new frontier. Journal of Ce-real Science, 102, 103370. https://doi.org/10.1016/j.jcs.2021.103370
  • Meints, B. & Hayes, P.M. (2019) Breeding naked barley for food, feed, and malt. In: Goldman, I. (ed) Plant Breeding Reviews, 43 (1). John Wiley & Sons, Inc., pp. 95-119.
  • Motoyoshi, F. (1967). Chlorophyll formation in several chlorophyll mutants of sand oats and barley cultured on nutrient media. The Japanese Journal of Genetics, 42(5), 291-297. https://doi.org/10.1266/jjg.42.291
  • Nazarenko, M. M., & Lykholat, T. Y. (2020). Variability at winter wheat varieties first generation which obtained mutagen action. Ecology and Noospherology, 31(2), 77-81. https://doi.org/10.15421/032012 Newman, R.K. & Newman, C.W. (2008) Barley for food and health: Science, technology, and products. John Wiley & Sons, Inc., 243, New Jersey.
  • Patial, M., Thakur, S. R., Singh, K. P., & Thakur, A. (2017). Frequency and spectrum of chlorophyll mutations and induced variability in ricebean (Vigna umbellata Thunb, Ohwi and Ohashi). Legume Research-An International Journal, 40(1), 39-46. https://doi.org/10.18805/lr.v0iOF. 10757
  • Sakin, M.A. & Sencar, Ö. (2001). Makarnalık buğday (Triticum durum Desf.) çeşitlerinin mutagenlere tepkileri. Journal of Agricultural Faculty of Gaziosmanpaşa University, 18(1), 89-93. https://dergipark.org.tr/tr/pub/gopzfd/issue/7349/96160 (in Turkish)
  • Simpson, D. J., Machold, O., Høyer-Hansen, G., & Von Wettstein, D. (1985). Chlorina mutants of barley (Hordeum vulgare L.). Carlsberg Research Communications, 50, 223-238. https://doi.org/10.1007/BF02907148 Singh, R. M., Singh, J., & Srivastava, A. N. (1977). Mutagenic effects of gamma rays, EMS, and HA in barley. Barley Genetics Newsletter, 7, 60.
  • Ullrich, S.E. (2011). Significance, adaptation, production, and trade of barley. In: Ullrich, S.E. (ed.). Barley, Blackwell Publishing Ltd., , Chichester, UK. pp. 3-13
  • Ünver, S. (1989). The effect of different doses of EMS and the temperatures and periods of post washing applied on the some characters in M1 and M2 plants of barley (Hordeum vulgare L.) (in Turkish) (Unpublished PhD thesis). Ankara University, Ankara, Turkey.
  • Wani, M. R., Khan, S., & Kozgar, M. I. (2011). Induced chlorophyll mutations. I. Mutagenic effectiveness and efficiency of EMS, HZ and SA in mungbean. Frontiers of Agriculture in China, 5, 514-518. https://doi.org/10.1007/s11703-011-1126-y
  • Wani, M.R. 2017. Induced chlorophyll mutations, comparative mutagenic effectiveness and efficiency of chemical mutagens in lentils (Lens culinaris Medik). Asian J. Plant Sci., 16: 221-226. https://doi.org/10.3923/ajps.2017.221.226
  • Yu, K., Lenz-Wiedemann, V., Chen, X., & Bareth, G. (2014). Estimating leaf chlorophyll of barley at different growth stages using spectral indices to reduce soil background and canopy structure effects. ISPRS Journal of Photogrammetry and Remote Sensing, 97, 58-77. https://doi.org/10.1016/j.isprsjprs.2014.08.005
There are 32 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Makaleler
Authors

Namuk Ergün 0000-0002-6491-4149

Güray Akdogan 0000-0002-1192-3615

Saime İkincikarakaya 0000-0001-8561-9002

Early Pub Date December 8, 2023
Publication Date December 22, 2023
Submission Date April 24, 2023
Published in Issue Year 2023 Volume: 9 Issue: 4

Cite

APA Ergün, N., Akdogan, G., & İkincikarakaya, S. (2023). Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes. Journal of Advanced Research in Natural and Applied Sciences, 9(4), 923-930. https://doi.org/10.28979/jarnas.1286891
AMA Ergün N, Akdogan G, İkincikarakaya S. Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes. JARNAS. December 2023;9(4):923-930. doi:10.28979/jarnas.1286891
Chicago Ergün, Namuk, Güray Akdogan, and Saime İkincikarakaya. “Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes”. Journal of Advanced Research in Natural and Applied Sciences 9, no. 4 (December 2023): 923-30. https://doi.org/10.28979/jarnas.1286891.
EndNote Ergün N, Akdogan G, İkincikarakaya S (December 1, 2023) Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes. Journal of Advanced Research in Natural and Applied Sciences 9 4 923–930.
IEEE N. Ergün, G. Akdogan, and S. İkincikarakaya, “Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes”, JARNAS, vol. 9, no. 4, pp. 923–930, 2023, doi: 10.28979/jarnas.1286891.
ISNAD Ergün, Namuk et al. “Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes”. Journal of Advanced Research in Natural and Applied Sciences 9/4 (December 2023), 923-930. https://doi.org/10.28979/jarnas.1286891.
JAMA Ergün N, Akdogan G, İkincikarakaya S. Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes. JARNAS. 2023;9:923–930.
MLA Ergün, Namuk et al. “Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes”. Journal of Advanced Research in Natural and Applied Sciences, vol. 9, no. 4, 2023, pp. 923-30, doi:10.28979/jarnas.1286891.
Vancouver Ergün N, Akdogan G, İkincikarakaya S. Investigation of Chlorophyll Mutations in Gamma Irradiated Naked Barley Genotypes. JARNAS. 2023;9(4):923-30.
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