Research Article
Pleurotus eryngii Mantarı Kullanılarak Elde Edilen Nanofungal Moleküllerin Antibakteriyal ve Antifungal Aktivitesi
Abstract
Nano düzeyde molekül sentezi, tedavi eksenli ve çözüm odaklı uygulamaları nedeniyle her geçen gün büyüyen bir bilim alanı haline gelmektedir. Özellikle bitkiler ve bakteriler nanoparçacık üretiminde önemli bir yere sahiptir. Son yıllarda nanofungal yapıların bilimsel çalışmalarda olumlu sonuçlar vermesi, bilim dünyasının mantar yapılı nanomolekül sentezine yönelmesine neden olmuştur. Gümüş kaplı nanofungal yapılar tıbbi ve endüstriyel uygulamalarda çok fazla tercih edilmektedir. Yapmış olduğumuz çalışmada Pleurotus eryngii (Heliz Mantarı) ve AgNO3 kullanılarak elde edilen nano moleküllerin Disk difüzyon metodu kullanılarak Antibakteriyal ve Antifungal aktivitesi araştırıldı. Çalışmada 10 farklı klinik suş kullanıldı. Elde edilen sonuçlara bakıldığında nano moleküllerin kullanılan patojen mikroorganizmalara karşı 9.1-15.0 arasında değişen oranlarda zonlar oluşturduğu belirlendi. Ayrıca nanoparçacıkların bazı patojenlere karşı pozitif kontrol olarak kullanılan antibiyotiklerden daha etkili zonlar oluşturduğu görüldü.
References
- Akyüz, M., Kırbağ, S., 2007. Ülkemizde sebze ve meyvelerin yanı sıra alternatif besin kaynağı: Yabani mantar (Pleurotus eryngii var. ferulae). Artvin Çoruh Üni Orman Fak Derg, 8(1), 26-36.
- Chaudhuri, R.G., Paria. S., 2012. A novel method for the templated synthesis of Ag2S hollow nanospheres in aqueous surfactant media. J. Colloid. Inter. Sci. 369, 117-122.
- Gregori, A., Švagelj, M., Pohleven, J., 2007. Cultivation techniques and medicinal properties of Pleurotus spp. Food Technol. Biotechnol. 45, 238-249.
- Gupta, K., Shabiha, N.H., Devabrata, S., Nima, D.N., Manabendra, M., 2014. One stepgreen synthesis and antimicrobial and antibiofilm properties of Psidium guajava L. leaf extract-mediated silver nanoparticles. Mater. Lett. 125, 67-70.
- Kavyashree, D., Ananda-Kumari, R., Nagabhushana, H., Sharma, S.C., Vidya, Y.S., Anantharaju, K.S., Daruka-Prasad, B., Prashantha, S.C., Lingaraju, K., Rajanaik, H., 2015. Orange red emitting Eu3 + doped zinc oxide nanophosphor material prepared using Guizotia abyssinica seed extract: structural and pho-toluminescence studies. J. Lumin. 167, 91-100.
- Kumar, H., Bhardwaj, K., Nepovimova, E., Kuča, K., Singh-Dhanjal, D., Bhardwaj, S., Bhatia, S.K., Verma, R., Kumar, D., 2020. Antioxidant Functionalized Nanoparticles: A Combat against Oxidative Stress. Nanomaterials (Basel), 10.
- Kumar, V., Yadav, S.K., 2009. Plant-mediated synthesis of silver and gold nanoparticles and their applications. J. Chem. Technol. Biotechnol. 84(2), 151-157.
- Lin, J.T., Liu, C.W., Chen, Y.C., Hu, C.C., Juang, L.D., Shiesh, C.C., Yang, D.J., 2014. Chemical composition, antioxidant and antiinflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time. LWT-Food Science and Technology. 55(1), 374-382.
- Mallmann EJJ, Cunha FA, Castro BNMF, Maciel AM, Menezes EA, Fechine PBA, (2015). Antifungal activity of silver nanoparticles obtained by green synthesis. Rev. Inst. Med. Trop. Sao Paulo, 57: 165-167.
- Markowska, K., Grudniak, A.M., Wolska, K.I., 2013. Silver nanoparticles as an alternative strategy against bacterial biofilms. Acta Biochim. Pol. 60, 523-530.
- Maurya, S., Bhardwaj, A.K., Gupta, K.K., Agarwal, S., Kushwaha, A., et al. 2016. Green Synthesis of Silver Nanoparticles using Pluerotus and its Bactericidal Activity. Cell Mol. Biol. 62, 131.
- Meydan, İ., 2021. Investigation of Antioxidant, Antimicrobial and DNA Damage Prevention Activity of the Diplotaenia turcica Plant Stabilized Silver Nanoparticles. Fres Env Bull, 30(2), 1231-1239.
- Meydan, İ., Seckin, H., 2021. Green synthesis, characterization, antimicrobial and antioxidant activities of zinc oxide nanoparticles using Helichrysum arenarium extract. Int. J. of Agr. Env. and Food Sci. 5(1), 33-41.
- Mohanpuria, P., Rana, N.K., Yadav, S.K., 2008. Biosynthesis of nanoparticles: Technological concepts and future applications. J. Nanopart. Res. 10, 507-517.
- Moonmoon, M., Uddin, N.M., Ahmed, S., Shelly, N., 2010. Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on sawdust and rice straw in Bangladesh. Saudi Journal of Biological Sciences. 17(4), 341-345.
- Muszyńska, B., Grzywacz-Kisielewska, A., Kała, K., Gdula-Argasińska, J., 2018. Anti-inflammatory properties of edible mushrooms: A review. Food Chem. 243, 373-381.
- Prakash, P., Gnanaprakasam, P., Emmanuel, R., Arokiyaraj, S., Saravanan, M., 2013. Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf. B: Biointerfaces. 108, 255-259.
- Parashar, V., Parashar, R., Sharma, B., Pandey, A.C., 2009. Parthenium leaf extract mediated synthesis of silver nanoparticles: a novel approach towards weed utilization. Dig. J. Nanomater. Bios. 4, 45-50.
- Sun, Q., Xiang, C., Jiangwei, L., Zheng, M., Chen, Z., Chang-Ping, Y., 2014. Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids and Surfaces A: Physicochem. Eng. Aspects. 444, 226-231.
- Radhakrishnan, M.C., Peter, P., 2021. Biological Properties of Silver Nanoparticles from β (1-3) Glucan Isolated from the Edible Mushroom Pleurotus florida. Letters in Applied Nano. Bio. Science. 10(2), 2096-2106.
- Rodriguez Estrada, A.E., 2008. Molecular phylogeny and increases of yield and the antioxidants selenium and ergothioneine in Basidiomata of Pleurotus eryngii, PhD Thesis, Pennsylvania State University, Department of Plant Pathology. 237 p.
- Salem, W., Leitner. D.R., Zingl, F.G., Schratter. G., Prassl, R., Goessler, W., Reidl, J., Schild, S., 2015. Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoxic Escherichia coli. Int. J. Med. Microbiol. 305, 85-95.
- Seçkin, H., Meydan, İ., 2021. Synthesis and Characterization of Veronica beccabunga Green Synthesized Silver Nanoparticles for The Antioxidant and Antimicrobial Activity Turk J. Agric. Res. 8(1), 49-55.
- Selvi, K.V., Sivakumar, T., 2014. Synthesis and Characterization of Silver Nanoparticles from Fusarium oxysporum Research Article. International Journal of Advanced Research in Biological Sciences. 1(2), 115-123.
- Senthilkumar, S.R., Sivakumar, T., 2014. Green tea (Camellia sinensis) mediated synthesis of zinc oxide (ZnO) nanoparticles and studies on their antimicrobial activities. International Journal of Pharmacy and Pharmaceutical Sciences. 6(6), 461-465.
- Staji, M., Vukojevi, J., Duleti-Lauševi, S., 2009. Biology of Pleurotus eryngii and role in biotechnological processes: a review. Critical Reviews in Biotechnology. 29(1), 55-66.
- Syed, I.Z., Shaikh, R., Bhende, P., 2019. Green synthesis of silver nanoparticles using root extracts of Cassia toral L. and its antimicrobial activities. Asian Journal of Green Chemistry, 3(1), 70-81.
Year 2021,
Volume: 4 Issue: 1, 16 - 23, 30.06.2021
Abstract
Molecular synthesis at nano level is becoming a growing field of science due to its treatment and solution-oriented applications. Plants and bacteria in particular have an important place in nanoparticle production. The positive results of nanofungal structures in scientific studies in recent years have led the scientific world to turn to fungal nanomolecule synthesis. Silver coated nanofungal structures are highly preferred in medical and industrial applications. In our study, the Antibacterial and Antifungal activity of nano molecules obtained by using Pleurotus eryngii (Heliz Mushroom) and AgNO3 was investigated by using Disk diffusion method. Ten different clinical strains were used in the study. Looking at the results obtained, it was determined that nano molecules formed zones varying between 9.1-15.0 against pathogenic microorganisms used. In addition, it was observed that nanoparticles formed zones more effective against some pathogens than antibiotics used as positive control.
References
- Akyüz, M., Kırbağ, S., 2007. Ülkemizde sebze ve meyvelerin yanı sıra alternatif besin kaynağı: Yabani mantar (Pleurotus eryngii var. ferulae). Artvin Çoruh Üni Orman Fak Derg, 8(1), 26-36.
- Chaudhuri, R.G., Paria. S., 2012. A novel method for the templated synthesis of Ag2S hollow nanospheres in aqueous surfactant media. J. Colloid. Inter. Sci. 369, 117-122.
- Gregori, A., Švagelj, M., Pohleven, J., 2007. Cultivation techniques and medicinal properties of Pleurotus spp. Food Technol. Biotechnol. 45, 238-249.
- Gupta, K., Shabiha, N.H., Devabrata, S., Nima, D.N., Manabendra, M., 2014. One stepgreen synthesis and antimicrobial and antibiofilm properties of Psidium guajava L. leaf extract-mediated silver nanoparticles. Mater. Lett. 125, 67-70.
- Kavyashree, D., Ananda-Kumari, R., Nagabhushana, H., Sharma, S.C., Vidya, Y.S., Anantharaju, K.S., Daruka-Prasad, B., Prashantha, S.C., Lingaraju, K., Rajanaik, H., 2015. Orange red emitting Eu3 + doped zinc oxide nanophosphor material prepared using Guizotia abyssinica seed extract: structural and pho-toluminescence studies. J. Lumin. 167, 91-100.
- Kumar, H., Bhardwaj, K., Nepovimova, E., Kuča, K., Singh-Dhanjal, D., Bhardwaj, S., Bhatia, S.K., Verma, R., Kumar, D., 2020. Antioxidant Functionalized Nanoparticles: A Combat against Oxidative Stress. Nanomaterials (Basel), 10.
- Kumar, V., Yadav, S.K., 2009. Plant-mediated synthesis of silver and gold nanoparticles and their applications. J. Chem. Technol. Biotechnol. 84(2), 151-157.
- Lin, J.T., Liu, C.W., Chen, Y.C., Hu, C.C., Juang, L.D., Shiesh, C.C., Yang, D.J., 2014. Chemical composition, antioxidant and antiinflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time. LWT-Food Science and Technology. 55(1), 374-382.
- Mallmann EJJ, Cunha FA, Castro BNMF, Maciel AM, Menezes EA, Fechine PBA, (2015). Antifungal activity of silver nanoparticles obtained by green synthesis. Rev. Inst. Med. Trop. Sao Paulo, 57: 165-167.
- Markowska, K., Grudniak, A.M., Wolska, K.I., 2013. Silver nanoparticles as an alternative strategy against bacterial biofilms. Acta Biochim. Pol. 60, 523-530.
- Maurya, S., Bhardwaj, A.K., Gupta, K.K., Agarwal, S., Kushwaha, A., et al. 2016. Green Synthesis of Silver Nanoparticles using Pluerotus and its Bactericidal Activity. Cell Mol. Biol. 62, 131.
- Meydan, İ., 2021. Investigation of Antioxidant, Antimicrobial and DNA Damage Prevention Activity of the Diplotaenia turcica Plant Stabilized Silver Nanoparticles. Fres Env Bull, 30(2), 1231-1239.
- Meydan, İ., Seckin, H., 2021. Green synthesis, characterization, antimicrobial and antioxidant activities of zinc oxide nanoparticles using Helichrysum arenarium extract. Int. J. of Agr. Env. and Food Sci. 5(1), 33-41.
- Mohanpuria, P., Rana, N.K., Yadav, S.K., 2008. Biosynthesis of nanoparticles: Technological concepts and future applications. J. Nanopart. Res. 10, 507-517.
- Moonmoon, M., Uddin, N.M., Ahmed, S., Shelly, N., 2010. Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on sawdust and rice straw in Bangladesh. Saudi Journal of Biological Sciences. 17(4), 341-345.
- Muszyńska, B., Grzywacz-Kisielewska, A., Kała, K., Gdula-Argasińska, J., 2018. Anti-inflammatory properties of edible mushrooms: A review. Food Chem. 243, 373-381.
- Prakash, P., Gnanaprakasam, P., Emmanuel, R., Arokiyaraj, S., Saravanan, M., 2013. Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf. B: Biointerfaces. 108, 255-259.
- Parashar, V., Parashar, R., Sharma, B., Pandey, A.C., 2009. Parthenium leaf extract mediated synthesis of silver nanoparticles: a novel approach towards weed utilization. Dig. J. Nanomater. Bios. 4, 45-50.
- Sun, Q., Xiang, C., Jiangwei, L., Zheng, M., Chen, Z., Chang-Ping, Y., 2014. Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids and Surfaces A: Physicochem. Eng. Aspects. 444, 226-231.
- Radhakrishnan, M.C., Peter, P., 2021. Biological Properties of Silver Nanoparticles from β (1-3) Glucan Isolated from the Edible Mushroom Pleurotus florida. Letters in Applied Nano. Bio. Science. 10(2), 2096-2106.
- Rodriguez Estrada, A.E., 2008. Molecular phylogeny and increases of yield and the antioxidants selenium and ergothioneine in Basidiomata of Pleurotus eryngii, PhD Thesis, Pennsylvania State University, Department of Plant Pathology. 237 p.
- Salem, W., Leitner. D.R., Zingl, F.G., Schratter. G., Prassl, R., Goessler, W., Reidl, J., Schild, S., 2015. Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoxic Escherichia coli. Int. J. Med. Microbiol. 305, 85-95.
- Seçkin, H., Meydan, İ., 2021. Synthesis and Characterization of Veronica beccabunga Green Synthesized Silver Nanoparticles for The Antioxidant and Antimicrobial Activity Turk J. Agric. Res. 8(1), 49-55.
- Selvi, K.V., Sivakumar, T., 2014. Synthesis and Characterization of Silver Nanoparticles from Fusarium oxysporum Research Article. International Journal of Advanced Research in Biological Sciences. 1(2), 115-123.
- Senthilkumar, S.R., Sivakumar, T., 2014. Green tea (Camellia sinensis) mediated synthesis of zinc oxide (ZnO) nanoparticles and studies on their antimicrobial activities. International Journal of Pharmacy and Pharmaceutical Sciences. 6(6), 461-465.
- Staji, M., Vukojevi, J., Duleti-Lauševi, S., 2009. Biology of Pleurotus eryngii and role in biotechnological processes: a review. Critical Reviews in Biotechnology. 29(1), 55-66.
- Syed, I.Z., Shaikh, R., Bhende, P., 2019. Green synthesis of silver nanoparticles using root extracts of Cassia toral L. and its antimicrobial activities. Asian Journal of Green Chemistry, 3(1), 70-81.
There are 27 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | June 30, 2021 |
| Submission Date | March 30, 2021 |
| Acceptance Date | June 3, 2021 |
| Published in Issue | Year 2021 Volume: 4 Issue: 1 |
