Research Article
Investigation on marine Staphylococcus spp. isolated from the Sinop coastal areas, the Black Sea in Türkiye serves as a reservoir for antibiotic resistance genes
Abstract
Epidemiological surveillance of the Staphylococci genus, which harbours strains with high morbidity and mortality rates, is a crucial step in detecting and preventing diseases and disease agents. This study aimed to isolate, characterise, and screen some antibiotic resistance genes of possible Staphylococcus spp. strains from seawater samples taken from three points in Sinop, which is suitable for swimming from almost every point. Classical microbiological techniques were used for the isolation and possible identification of the strains. A fragment of the 16S rRNA gene region (216 bp) was amplified and analysed by the SSCP technique to determine their diversity among themselves. For antibiotic resistance genes, both classical PCR and multiplex PCR techniques were used. As a result, 29 probable Staphylococcus spp. strains were isolated, and according to SSCP analysis, it was determined that the strains had a similarity rate of 50% or more among themselves and within the scope of different stations. In addition, mecA, ermA, ermB, ermC, tetK, tetM, and blaZ resistance genes of the strains were observed as 8 (27.5%), 3 (10.3%), 2 (6.8%), 2 (6.8%), 14 (48.2%), 27 (93.1%) and 29 (100%), respectively. Furthermore, mecA was positively correlated with ermB and ermB was positively correlated with ermC at the p < 0.05 significance level. In comparison, ermB was negatively correlated with tetM at the p < 0.05 significance level. In conclusion, the presence of Staphylococcus spp. strains, which are reservoirs of antibiotic resistance genes and have the potential to transfer these genes to other bacteria through gene transfer, have been shown in this study to be prevalent in marine environments, where they can be easily transmitted. The importance of taking precautions has been emphasised.
Ethical Statement
Ethics committee approval is not required for this study.
Project Number
No project support was received for the study.
References
- Ackers-Johnson, G., Pulmones, R., McLaughlan, D., Doyle, A., Lewis, J.M., Neal, T., Roberts, A.P. (2024). Investigating the changing taxonomy and antimicrobial resistance of bacteria isolated from door handles in a new infectious disease ward pre-and post-patient admittance. Microbiology Spectrum, 12(12), e01797-24. https://doi.org/10.1128/spectrum.01797-24
- Agredo-Campos, Á.S., Camussone, C., Akineden, Ö., Fernández-Silva, J.A., Ramírez-Vásquez, N.F. (2025). Pheno-and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia. International Dairy Journal, 160, 106088. https://doi.org/10.1016/j.idairyj.2024.106088
- Akanbi, O.E., Njom, H.A., Fri, J., Otigbu, A.C., Clarke, A.M. (2017). Antimicrobial susceptibility of Staphylococcus aureus isolated from recreational waters and beach sand in Eastern Cape Province of South Africa. International Journal of Environmental Research and Public Health, 14(9), 1001. https://doi.org/10.3390/ijerph14091001
- Alabbosh, K.F., Zmantar, T., Bazaid, A.S., Snoussi, M., Noumi, E. (2023). Antibiotics resistance and adhesive properties of clinical Staphylococcus aureus isolated from wound infections. Microorganisms, 11(5), 1353. https://doi.org/10.3390/microorganisms11051353
- Alkuraythi, D.M., Alkhulaifi, M.M., Binjomah, A.Z., Alarwi, M., Mujallad, M.I., Alharbi, S.A., Alajel, S.M. (2024). Comparative genomic analysis of antibiotic resistance and virulence genes in Staphylococcus aureus isolates from patients and retail meat. Frontiers in Cellular and Infection Microbiology, 13, 1339339. https://doi.org/10.3389/fcimb.2023.1339339
- Avşar, C., Koyuncu, H., Aras, E.S. (2017). Isolation and molecular characterization of Bacillus spp. isolated from soil for production of industrial enzymes. Biological and Chemical Research, 3(9), 72-86.
- Brauge, T., Bourdonnais, E., Trigueros, S., Cresson, P., Debuiche, S., Granier, S.A., Midelet, G. (2024). Antimicrobial resistance and geographical distribution of Staphylococcus sp. isolated from whiting (Merlangius merlangus) and seawater in the English Channel and the North Sea. Environmental Pollution, 345, 123434. https://doi.org/10.1016/j.envpol.2024.123434
- Contractor, S.S., Kulkarni, R.D., Arakera, S.B. (2024). Prevalence, antibiotic susceptibility pattern and the detection of mecA gene among Staphylococcus spp. isolates from a tertiary care hospital in North Karnataka. Indian Journal of Microbiology, 1-9. https://doi.org/10.1007/s12088-024-01403-6
- Cui, M., Wang, H., Li, Z., Han, N., Li, J., Peng, W., Wang, X. (2024). Phenotypic and molecular characterization of Staphylococcus aureus in dairy farms from Henan Province and the Inner Mongolia Autonomous Region, China. Microorganisms, 12(11), 2150. https://doi.org/10.3390/microorganisms12112150
- Desire, Z., Comoé, B., Donatien, K., Koua, A., Naka, T., Adjaratou, T., Dosso, M. (2024). Phenotypic and genotypic characterization of mecA gene in methicillin-resistant Staphylococcus aureus isolated from smoked fish. Advances in Microbiology, 14(12), 605-617. https://doi.org/10.4236/aim.2024.1412043
- Dewi, D.A.R., Khalifa, H.O., Khandar, H., Hisatsune, J., Kutuno, S., Yu, L., Matsumoto, T. (2024). Detection and genetic characterization of multidrug-resistant staphylococci isolated from public areas in an international airport. Scientific Reports, 14(1), 27738. https://doi.org/10.1038/s41598-024-79447-3
- Duran, N., Ozer, B., Duran, G. G., Onlen, Y., Demir, C. (2012). Antibiotic resistance genes & susceptibility patterns in staphylococci. Indian Journal of Medical Research, 135(3), 389-396.
- Faria, C., Vaz-Moreira, I., Serapicos, E., Nunes, O.C., Manaia, C.M. (2009). Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water. Science of the Total Environment, 407(12), 3876-3882. https://doi.org/10.1016/j.scitotenv.2009.02.034
- Fontana, C., Patrone, V., Lopez, C. M., Morelli, L., Rebecchi, A. (2021). Incidence of tetracycline and erythromycin resistance in meat-associated bacteria: Impact of different livestock management strategies. Microorganisms, 9(10), 2111. https://doi.org/10.3390/microorganisms9102111
- Gerken, T.J., Roberts, M.C., Dykema, P., Melville, P.A., de Mattos-Guaraldi, A.L., dos Santos, K.R.N. (2024). Prevalence and characterization of antimicrobial resistance genes in Staphylococcus spp. isolated from clinical and environmental sources. Microbial Drug Resistance, 30(1), 123-134. Goodwin, K.D., McNay, M., Cao, Y., Ebentier, D., Madison, M., Griffith, J.F. (2012). A multi-beach study of Staphylococcus aureus, MRSA, and enterococci in seawater and beach sand. Water Research, 46(13), 4195-4207. https://doi.org/10.1016/j.watres.2012.04.001
- Harakeh, S., Yassine, H., Hajjar, S., El-Fadel, M. (2006). Isolates of Staphylococcus aureus and Staphylococcus saprophyticus resistant to antimicrobials isolated from the Lebanese aquatic environment. Marine Pollution Bulletin, 52(8), 912-919. https://doi.org/10.1016/j.marpolbul.2005.12.008
- Heras, J., Domínguez, C., Mata, E., Pascual, V., Lozano, C., Torres, C., Zarazaga, M. (2015). GelJ–a tool for analyzing DNA fingerprint gel images. BMC Bioinformatics, 16, 270. https://doi.org/10.1186/s12859-015-0703-0
- Hnini, R., Silva, E., Pinho, L., Najimi, M., Thompson, G. (2024). Beta-lactam antimicrobials activity and the diversity of blaZ gene in Staphylococcus aureus isolates from bovine mastitis in the northwest of Portugal. Revista Brasileira de Zootecnia, 53, e20230024. https://doi.org/10.37496/rbz5320230024
- Lee, J.H. (2003). Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied and Environmental Microbiology, 69(11), 6489-6494. https://doi.org/10.1128/AEM.69.11.6489-6494.2003
- Li, B., Yu, M., Ma, S., Huang, Y., Zhang, N., Chen, S. (2025). Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification. Journal of Analytical Science and Technology, 16(1), 1. https://doi.org/10.1186/s40543-024-00473-x
- Michalik, M., Podbielska-Kubera, A., Dmowska-Koroblewska, A. (2025). Antibiotic resistance of Staphylococcus aureus strains—searching for new antimicrobial agents. Pharmaceuticals, 18(1), 81. https://doi.org/10.3390/ph18010081
- Olivo, G., Zakia, L.S., Ribeiro, M.G., de Souza, M.D.L.R., Riboli, D.F.M., Mello, P.L., Borges, A.S. (2024). Methicillin-resistant Staphylococcus spp. investigation in hospitalized horses and contacting personnel in a teaching veterinary hospital. Journal of Equine Veterinary Science, 134, 105031. https://doi.org/10.1016/j.jevs.2024.105031
- Oshamika, O., Sonowo, O., Akinbolagbe, Y., Odusote, O., Akinnola, O., Eni, A. (2024). Nasal carriage, antimicrobial susceptibility profile, and enterotoxin genes of Staphylococcus aureus isolated from children with asthma. Indian Journal of Microbiology, 64(1), 1-9. https://doi.org/10.1007/s12088-024-01272-z
- Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Edition). Cold Spring Harbor Laboratory, New York.
- Szemraj, M., Glajzner, P., Olszowiec, K., Sienkiewicz, M. (2025). The prevalence of multidrug resistance in Staphylococcus hominis isolated from clinical materials. Scientific Reports, 15(1), 414. https://doi.org/10.1038/s41598-024-84500-2
- Soge, O.O., Meschke, J.S., No, D.B., Roberts, M.C. (2006). Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches. Journal of Antimicrobial Chemotherapy, 64(6), 1148-1155. https://doi.org/10.1093/jac/dkp368
- Takahashi, T., Kim, H., Kim, H. S., Kim, H.S., Song, W., Kim, J.S. (2024). Comparative genomic analysis of Staphylococcal Cassette Chromosome mec Type V Staphylococcus aureus strains and estimation of the emergence of SCCmec V clinical isolates in Korea. Annals of Laboratory Medicine, 44(1), 47-55. https://doi.org/10.3343/alm.2024.44.1.47
- Vittorakis, E., Vica, M.L., Zervaki, C.O., Vittorakis, E., Maraki, S., Mavromanolaki, V. E., Junie, L.M. (2024). A comparative analysis of MRSA: Epidemiology and antibiotic resistance in Greece and Romania. International Journal of Molecular Sciences, 25(14), 7535. https://doi.org/10.3390/ijms25147535
- Wan, L., Feng, L., Wang, M., Yang, Y., Pan, P., Gao, S. (2025). Proximity ligation-triggered DNAzyme for selective fluorescent aptasensing of methicillin-resistant Staphylococcus aureus. Analytical Biochemistry, 696, 115683. https://doi.org/10.1016/j.ab.2024.115683
- Yang, T., Zhang, X., Yan, Y., Liu, Y., Lin, X., Li, W. (2025). Artificial intelligence-driven quantification of antibiotic-resistant bacteria in food by color-encoded multiplex hydrogel digital LAMP. Food Chemistry, 468, 142304. https://doi.org/10.1016/j.foodchem.2024.142304
- Zhang, S., Wang, S., Sun, B., Chen, S., Ma, Q., Han, K., Jiang, H. (2025). A smartphone-based electrochemical/visible light dual-readout biosensor for sensitive detection of MRSA by self-assembly AuNPs@Nisin. Sensors and Actuators B: Chemical, 425, 137000. https://doi.org/10.1016/j.snb.2024.137000
- Zhao, X., Zeng, Y., Yan, B., Liu, Y., Qian, Y., Zhu, A., Wan, Z. (2024). A novel extraction-free dual HiFi-LAMP assay for detection of methicillin-sensitive and methicillin-resistant Staphylococcus aureus. Microbiology Spectrum, 12(4), e04133-23. https://doi.org/10.1128/spectrum.04133-23
There are 32 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Pollution and Contamination (Other) |
| Journal Section | Research Articles |
| Authors | |
| Project Number | No project support was received for the study. |
| Early Pub Date | June 25, 2025 |
| Publication Date | |
| Submission Date | February 21, 2025 |
| Acceptance Date | April 12, 2025 |
| Published in Issue | Year 2025Volume: 8 Issue: 3 |
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