Farklı LED ışıklar altında yetiştirilen köksüz su mercimeğinin (Wolffia arrhiza) Dumas yöntemi kullanılarak ham protein içeriğinin belirlenmesi

Beyhan Taş; Faruk Tolga Şengüllendi

doi:10.3153/AR23019

EN TR

Determination of crude protein content using the Dumas method of rootless duckweed (Wolffia arrhiza) grown under different LED lights

Abstract

Rootless duckweed (Wolffia spp.) has the potential high-protein food source for humans. Wolffia is a member of the Lemnaceae family and is the world's smallest spermatophyte, with reduced roots, stems, and leaves. Scientific research on Wolffia species is relatively new, and this species is believed to be rare in Turkey. The phytochemicals and nutritional composition of plants are significantly affected by environmental factors in the natural or cultivated medium. In this study, Wolffia arrhiza was collected and cultured from a local area in Yeşilırmak Delta (Samsun) grown under controlled conditions using different LED lights. The Dumas method was used to determine the amount of crude protein. The protein content, calculated by multiplying the nitrogen (%) content by the standard conversion factor 6.25, was also evaluated by using different conversion factors in the literature. The results showed that rootless duckweed can be successfully grown under artificial lighting conditions with different light spectrums. The protein content of rootless duckweed, which was around 10% in the natural environment, increased considerably under red LED light (41.6% protein). The effect of different lights on protein content of W. arrhiza was observed as red LED > blue LED > purple LED > fluorescent. With its high protein content, environmentally friendly and sustainable production, Wolffia has the potential to quickly take place in the plant-based protein and functional food market as an alternative to traditional crops in the near future.

Keywords

Farklı LED ışıklar altında yetiştirilen köksüz su mercimeğinin (Wolffia arrhiza) Dumas yöntemi kullanılarak ham protein içeriğinin belirlenmesi

Öz

Köksüz su mercimeği insanlar için potansiyel bir yüksek proteinli gıda kaynağıdır. Lemnaceae familyasının kök, gövde ve yaprakları indirgenmiş bir üyesi olan Wolffia, dünyanın en küçük spermatofitidir. Wolffia türleri ile yapılan bilimsel araştırmalar oldukça yeni olup, bu türün Türkiye’de nadir olduğu düşünülmektedir. Doğal ya da kültür ortamındaki çevresel faktörler, bitkilerin fitokimyasalları ve besinsel bileşimleri üzerinde önemli bir etki gösterir. Bu çalışmada, Yeşilırmak Deltası’nda (Samsun) lokal bir alandan toplanıp kültüre alınan Wolffia arrhiza, kontrollü koşullarda farklı LED ışıklar altında yetiştirilmiştir. Ham protein miktarının belirlenmesinde Dumas metodu kullanılmıştır. Azot (%) içeriğinin standart dönüşüm faktörü 6.25 ile çarpılmasıyla hesaplanan protein içeriği, literatürlerdeki farklı dönüşüm faktörleri kullanılarak da değerlendirilmiştir. Araştırma sonucunda, köksüz su mercimeğinin farklı ışık spektrumları ile yapay aydınlatma koşullarında başarılı bir şekilde yetiştirildiği kanıtlanmıştır. Doğal ortamdaki köksüz su mercimeğinin %10 civarında olan protein içeriği, kırmızı LED ışıkta oldukça yükselmiştir (%41.6 protein). Farklı ışıkların W. arrhiza protein içeriğine etkisi kırmızı LED > mavi LED > mor LED > floresan şeklinde gözlenmiştir. Yüksek protein içeriği, çevre dostu ve sürdürülebilir üretimi ile Wolffia, yakın gelecekte geleneksel mahsullere alternatif bir ürün olarak, bitki bazlı protein ve fonksiyonel gıda pazarında hızla yer alabilir potansiyele sahiptir.

Anahtar Kelimeler

Supporting Institution

Bu araştırma, Ordu Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir.

Project Number

Proje No: B-2310

Thanks

Türü tanımlayan ve doğal ortamdan köksüz su mercimeği örneklemesinde yardımcı olan Dr. Halim TOPALDEMİR’e teşekkür ederiz.

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Details

Primary Language

Turkish

Subjects

Hydrobiology

Journal Section

Research Article

Authors

Beyhan Taş ^*
0000-0001-6421-2561
Türkiye

Faruk Tolga Şengüllendi
0000-0002-2114-3778
Türkiye

Early Pub Date

June 27, 2023

Publication Date

July 17, 2023

Submission Date

April 11, 2023

Acceptance Date

June 3, 2023

Published in Issue

Year 1970 Volume: 6 Number: 3

DOI

https://doi.org/10.3153/AR23019

IZ

https://izlik.org/JA32RJ56KW

Cite

RIS / Bibtex

APA

Taş, B., & Şengüllendi, F. T. (2023). Farklı LED ışıklar altında yetiştirilen köksüz su mercimeğinin (Wolffia arrhiza) Dumas yöntemi kullanılarak ham protein içeriğinin belirlenmesi. Aquatic Research, 6(3), 189-200. https://doi.org/10.3153/AR23019