Research Article
Phylogenetic analysis of Luciobarbus Heckel, 1843 and Barbus Cuvier & Cloquet, 1816 species in the Euphrates River (Turkey) based on mtDNA COI gene sequences
Abstract
Natural fish species living in the Euphrates River System; It is subject to some pressures such as overfishing, competition with invasive species and habitat loss. As a result of these pressures, it leads to the decrease of endemic and native species. At the beginning of these species are the species belonging to the Barbus Cuvier & Cloquet, 1816 and Luciobarbus Heckel, 1843 genera, which have high economic importance. In this study, phylogenetic analysis of the species belonging to the genus Barbus Cuvier & Cloquet, 1816 and Luciobarbus Heckel, 1843, which live naturally in the Euphrates River, was carried out with mtDNA COI gene sequences. 17 fish samples belonging to five species from three localities belonging to the Euphrates river system (Turkey) were studied. Total DNA extraction was performed from muscle tissue using Commercial Kit. Then the mtDNA COI region was amplified by PCR and sequenced. Genetic distance values were calculated between 0.00201 and 0.15332, and it was determined that the closest species were L. xanthopterus and L. esocinus, and the most distant species were B. lacerta and A. grypus. In addition, phylogenetic analyzes of the target species were made and an phylogenetic tree was formed and the species were distinguished. In future studies, it is recommended to evaluate the data in this study, to determine the genetic characteristics of populations, and to carry out conservation studies at the population level.
Supporting Institution
Harran University (HUBAK)
Project Number
21070
References
- Cognetti, G., Maltagliati, F. (2000). Biodiversity and adaptive mechanisms in brackish water fauna. Marine Pollution Bulletin, 40, 7e14. https://doi.org/10.1016/S0025-326X(99)00173-3
- Darabi, A.R., Kashan, N., Fayazi, J., Aminafshar, M., Chamani, M. (2014). Investigation of phylogenetic relationship among two Barbus species (Cyprinidae) populations with mitochondrial DNA using PCR sequencing. International Journal of Biology, Pharmacy and Allied Sciences, 4 (2), 302-311.
- Frankham, R., Briscoe, D.A., Ballou, J.D. (2002). Introduction to Conservation Genetics. Cambridge University Press. https://doi.org/10.1017/CBO9780511808999
- Gozlan R.E., Britton J.R., Cowx I., Copp G.H. (2010). Current knowledge on non-native freshwater fish introductions. Journal of Fish Biology, 76, 751-786. https://doi.org/10.1111/j.1095- 8649.2010.02566.x
- Jackson M.C., Wasserman R.J., Grey J., Ricciardi A., Dick J.T.A., Alexander M.E. (2017). Novel and disrupted trophic links following invasion. Advances in Ecological Research, 57, 55–97. https://doi.org/10.1016/bs.aecr.2016.10.006
- Korkmaz, M. (2017). Türkiye'de yayılış gösteren Barbus spp. (Pisces: Cyprinidae) türlerinin coğrafik varyasyonlarının araştırılması. Doctor’s thesis, Hacettepe University, Graduate School of Natural and Applied Sciences, Department of Biology, pp: 125.
- Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547-1549. https://doi.org/10.1093/molbev/msy096
- Kuru, M. (1986). Dicle ve Fırat Nehirleri üzerinde kurulacak barajlarla soyu tehlikeye girecek balık türleri. VIII. Ulusal Biyoloji Kongresi, 3-5 Eylül 1986, İzmir. Cilt II Hidrobiyoloji Seksiyonu, 589-597.
- Liu, G., Zhou, L. (2016). Population genetic structure and molecular diversity of the red swamp crayfish in China based on mtDNA COI gene sequences. Mitochondrial DNA Part A, 28(6), 860-866. https://doi.org/10.1080/24701394.2016.1199022
- Manel, S., Guerin, P.E., Mouillot, D., Blanchet, S., Velez, L., Albouy, C., Pellissier, L. (2020). Global determinants of freshwater and marine fish genetic diversity. Nature Communications, 11(1), 692. https://doi.org/10.1038/s41467-020-14409-7
- Mollot, G., Pantel, J.H., Romanuk, T.N. (2017). The effects of invasive species on the decline in species richness: a global meta-analysis. Advances in Ecological Research, 56, 61-83. https://doi.org/10.1016/bs.aecr.2016.10.002
- Parmaksız, A. (2020). Population genetic diversity of yellow barbell (Carasobarbus luteus) from Kueik, Euphrates and Tigris Rivers based on mitochondrial DNA D-loop sequences. Turkish Journal of Fisheries and Aquatic Sciences, 20(1), 79-86. https://doi.org/10.4194/1303-2712-v20_1_08
- Parmaksız, A. (2021). Determination of genetic variations by using mitochondrial DNA cyt b sequences in populations of Carasobarbus luteus (Cyprinidae). Aquatic Research, 4(4), 313-320. https://doi.org/10.3153/AR21026
- Rock, J., Costa, F.O., Walker, D.I., North, A.W., Hutchinson, W.F., Carvalho, G.R. (2008). DNA Barcodes of Fish of The Scotia Sea, Antarctica Indicate Priority Groups for Taxonomic and Systematics Focus. Antarctic Science, 20(3), 253-262. https://doi.org/10.1017/S0954102008001120
- Spielman, D., Brook, B.W., Frankham, R. (2004). Most species are not driven to extinction before genetic factors impact them. Proceedings of the National Academy of Sciences, 101, 15261e15264. https://doi.org/10.1073/pnas.0403809101
- Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D.N. (2005). DNA Barcoding Australia's Fish Species, Philosophical Transactions of the Royal Society B-Biological Sciences, 360, 1847-1857. https://doi.org/10.1098/rstb.2005.1716
- Ünlü, E., Özbay, C., Kilic, A., Coskun, Y., Şeşen, R. (1997). GAP’ın faunaya etkileri. Türkiye Çevre Vakfı Yayını. No: 125, 79‐102.
- Xu, Z.H., Chen, J.L., Cheng, D.F., Liu, Y., Eric, F. (2011). Genetic variation among the geographic population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. Agricultural Sciences in China, 10, 1041- 1048.
- Xu, Z.H., Chen, J.L., Cheng, D.F., Liu, Y., Eric, F. (2011). Genetic variation among the geographic population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. Agricultural Sciences in China, 10, 1041-1048. https://doi.org/10.1016/S1671-2927(11)60092-8
Year 2022,
Volume: 5 Issue: 2, 129 - 135, 01.04.2022
Abstract
Project Number
21070
References
- Cognetti, G., Maltagliati, F. (2000). Biodiversity and adaptive mechanisms in brackish water fauna. Marine Pollution Bulletin, 40, 7e14. https://doi.org/10.1016/S0025-326X(99)00173-3
- Darabi, A.R., Kashan, N., Fayazi, J., Aminafshar, M., Chamani, M. (2014). Investigation of phylogenetic relationship among two Barbus species (Cyprinidae) populations with mitochondrial DNA using PCR sequencing. International Journal of Biology, Pharmacy and Allied Sciences, 4 (2), 302-311.
- Frankham, R., Briscoe, D.A., Ballou, J.D. (2002). Introduction to Conservation Genetics. Cambridge University Press. https://doi.org/10.1017/CBO9780511808999
- Gozlan R.E., Britton J.R., Cowx I., Copp G.H. (2010). Current knowledge on non-native freshwater fish introductions. Journal of Fish Biology, 76, 751-786. https://doi.org/10.1111/j.1095- 8649.2010.02566.x
- Jackson M.C., Wasserman R.J., Grey J., Ricciardi A., Dick J.T.A., Alexander M.E. (2017). Novel and disrupted trophic links following invasion. Advances in Ecological Research, 57, 55–97. https://doi.org/10.1016/bs.aecr.2016.10.006
- Korkmaz, M. (2017). Türkiye'de yayılış gösteren Barbus spp. (Pisces: Cyprinidae) türlerinin coğrafik varyasyonlarının araştırılması. Doctor’s thesis, Hacettepe University, Graduate School of Natural and Applied Sciences, Department of Biology, pp: 125.
- Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547-1549. https://doi.org/10.1093/molbev/msy096
- Kuru, M. (1986). Dicle ve Fırat Nehirleri üzerinde kurulacak barajlarla soyu tehlikeye girecek balık türleri. VIII. Ulusal Biyoloji Kongresi, 3-5 Eylül 1986, İzmir. Cilt II Hidrobiyoloji Seksiyonu, 589-597.
- Liu, G., Zhou, L. (2016). Population genetic structure and molecular diversity of the red swamp crayfish in China based on mtDNA COI gene sequences. Mitochondrial DNA Part A, 28(6), 860-866. https://doi.org/10.1080/24701394.2016.1199022
- Manel, S., Guerin, P.E., Mouillot, D., Blanchet, S., Velez, L., Albouy, C., Pellissier, L. (2020). Global determinants of freshwater and marine fish genetic diversity. Nature Communications, 11(1), 692. https://doi.org/10.1038/s41467-020-14409-7
- Mollot, G., Pantel, J.H., Romanuk, T.N. (2017). The effects of invasive species on the decline in species richness: a global meta-analysis. Advances in Ecological Research, 56, 61-83. https://doi.org/10.1016/bs.aecr.2016.10.002
- Parmaksız, A. (2020). Population genetic diversity of yellow barbell (Carasobarbus luteus) from Kueik, Euphrates and Tigris Rivers based on mitochondrial DNA D-loop sequences. Turkish Journal of Fisheries and Aquatic Sciences, 20(1), 79-86. https://doi.org/10.4194/1303-2712-v20_1_08
- Parmaksız, A. (2021). Determination of genetic variations by using mitochondrial DNA cyt b sequences in populations of Carasobarbus luteus (Cyprinidae). Aquatic Research, 4(4), 313-320. https://doi.org/10.3153/AR21026
- Rock, J., Costa, F.O., Walker, D.I., North, A.W., Hutchinson, W.F., Carvalho, G.R. (2008). DNA Barcodes of Fish of The Scotia Sea, Antarctica Indicate Priority Groups for Taxonomic and Systematics Focus. Antarctic Science, 20(3), 253-262. https://doi.org/10.1017/S0954102008001120
- Spielman, D., Brook, B.W., Frankham, R. (2004). Most species are not driven to extinction before genetic factors impact them. Proceedings of the National Academy of Sciences, 101, 15261e15264. https://doi.org/10.1073/pnas.0403809101
- Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D.N. (2005). DNA Barcoding Australia's Fish Species, Philosophical Transactions of the Royal Society B-Biological Sciences, 360, 1847-1857. https://doi.org/10.1098/rstb.2005.1716
- Ünlü, E., Özbay, C., Kilic, A., Coskun, Y., Şeşen, R. (1997). GAP’ın faunaya etkileri. Türkiye Çevre Vakfı Yayını. No: 125, 79‐102.
- Xu, Z.H., Chen, J.L., Cheng, D.F., Liu, Y., Eric, F. (2011). Genetic variation among the geographic population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. Agricultural Sciences in China, 10, 1041- 1048.
- Xu, Z.H., Chen, J.L., Cheng, D.F., Liu, Y., Eric, F. (2011). Genetic variation among the geographic population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. Agricultural Sciences in China, 10, 1041-1048. https://doi.org/10.1016/S1671-2927(11)60092-8
There are 19 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Hydrobiology |
| Journal Section | Research Articles |
| Authors | |
| Project Number | 21070 |
| Early Pub Date | January 28, 2022 |
| Publication Date | April 1, 2022 |
| Submission Date | January 21, 2022 |
| Published in Issue | Year 2022Volume: 5 Issue: 2 |
is licensed under a CreativeCommons Attribtion-ShareAlike 4.0 International Licence
Diamond Open Access refers to a scholarly publication model in which journals and platforms do not charge fees to either authors or readers.
Open Access Statement:
This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.
Archiving Policy:
Archiving is done according to TÜBİTAK ULAKBİM "DergiPark" publication policy (LOCKSS).