Research Article
BibTex RIS Cite

Polyphenolic composition and Antioxidant Effect of Aerial Parts and Roots Extracts from Scorzonera veratrifolia

Year 2021, Volume: 8 Issue: 3, 284 - 299, 10.09.2021

Duygu Taşkın Mert Geçim Ahmet Doğan Ayfer Beceren

https://doi.org/10.21448/ijsm.943707
Cited By: 4

Abstract

Antioxidant activities of the different extracts (n-heptane, chloroform, methanol) from the roots and aerial parts of Scorzonera veratrifolia by maceration method, as well as total phenolic and flavonoid content were examined first time in this study. The findings revealed that the methanol extract from S. veratrifolia aerial parts exhibited greater DPPH radical scavenging (IC50: 0.62±0.60 mg/mL) and iron (III) reduction capacity (1.56±0.03 mM Fe2+/mg extract). Furthermore, aerial parts methanol extract has the highest concentration of total phenolic (46.3±1.1 mgGAE/g extract) and flavonoid (0.013±0.002 mg QE/mg extract) compounds. Based on these findings, the main phenolic content of aerial parts methanol extract was analyzed by LC-ESI-QTOF/MS, as this extract was found to contain the strongest antioxidant as well as the highest amount of phenolics and flavonoids as compared to the others. Quinic acid, chlorogenic acid, rutin, liquiritin, quercetin hexoside, luteolin-7-O-rutinoside, and di-O-caffeoylquinic acid compounds were identified as major compounds in methanol extract. The findings showed that aerial parts of S. veratrifolia, rather than its roots, could be used as a source of antioxidants.

Keywords

Antioxidant LC-MS Scorzonera Phenolic

Supporting Institution

TÜBİTAK

Project Number

217S050

References

  • Acıkara, O.B., Cıtıoglu, G.S., & Ozkan, A.M.G. (2013). Qualitative and quantitative analysis of phenolic acids in Scorzonera tomentosa L. Turk J Pharm Sci., 10, 1-8.
  • Acıkara, O.B., Ergene, B., Bakar, F., Cıtoglu, G.S., & Nebioglu, S. (2017). Evaluation of Antioxidant Activities and Phenolic Compounds of Scorzonera latifolia (Fisch. & Mey.) DC. Collected from Different Geographic Origins in Turkey. Turk J Pharm Sci., 14, 179-184. http://dx.doi.org/10.4274/tjps.57070
  • Akyüz, E., Özyürek, M., Güçlü, K., & Apak, M.R. (2013). Novel pro-oxidant activity assay for polyphenols vitamins C and E using a modified CUPRAC method. Talanta., 115, 583–589. https://doi.org/10.1016/j.talanta.2013.06.006
  • Benzie, I.F., & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem., 239, 70 76. https://doi.org/10.1006/abio.1996.0292
  • Bursal, E., Taslimi, P., Goren, A.C., & Gulcin, I. (2020). Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua. Biocatal. Agric. Biotechnol., 28, 101711. https://doi.org/10.1016/j.bcab.2020.101711
  • Chedea, V.S., Braıcu, C., & Cocacıu, C. (2010). Antioxidant/prooxidant activity of a polyphenolic grape seed extract. Food Chemi., 121, 132 139. https://doi.org/10.1016/j.foodchem.2009.12.020
  • Erden, Y., Kırbag, S., & Yılmaz, O. (2013). Phytochemical Composition and Antioxidant Activity of Some Scorzonera Species. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci., 83, 271–276. http://dx.doi.org/10.1007/s40011-012-0129-7
  • Hallıwell, B. (2008). Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies?. Arch. Biochem. Biophys., 476, 107 112. https://doi.org/10.1016/j.abb.2008.01.028
  • Hofmann, T., Nebehaj, E., & Albert, L. (2016). Antioxidant properties and detailed polyphenol profiling of Europeanhornbeam (Carpinus betulus L.) leaves by multiple antioxidantcapacity assays and high performance liquid chromatography/multistage electrospray mass spectrometry. Ind Crop Prod., 87, 340 349. https://doi.org/10.1016/j.indcrop.2016.04.037
  • Huyut, Z., Beydemir, Ş., & Gülçin, İ. (2017). Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem Res Int., 2017, 10. https://doi.org/10.1155/2017/7616791
  • Karagöz, A., Artuna, F.T., Ozcan, G., Melikoglu, G., Anıl, S., Kultur, S., & Sutlupınar, N. (2015). In vitro evaluation of antioxidant activity of some plant methanol extracts. Biotechnol Biotec EQ., 29, 1184-1189. http://dx.doi.org/10.1080/13102818.2015.1080600
  • Nasseri, M.A., Bigy, S.S., Allahresani, A., & Malekaneh, M. (2015). Assessment of Antioxidant Activity, Chemical Characterization and Evaluation of Fatty Acid Compositions of Scorzonera paradoxa Fisch and C. A. Mey. Jundishapur J Nat Pharm Prod., 10, 19781. http://dx.doi.org/10.17795/jjnpp-19781
  • Papettı, A., Daglıa, M., Grısolı, P., Dacarro, C., Gregottı, C., & Gazzanı, G. (2006). Anti- and pro-oxidant activity of Cichorium genus vegetables and effect of thermal treatment in biological systems. Food Chem., 97, 157 165. https://doi.org/10.1016/j.foodchem.2005.03.036
  • Samatha, T., Shyamsundarachary, R., Srinivas, P., & Swamy, N.R. (2012). Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian J. Pharmaceut. Clin. Res., 5, 177–179.
  • Sarı, A. (2010). Two new 3-benzylphthalides from Scorzonera veratrifolia Fenzl. Nat Prod Res. 24, 56-62. http://dx.doi.org/10.1080/14786410902800699
  • Sarı, A., Ozbek, B., & Ozgokce, F. (2009). Antimicrobial activities of two Scorzonera species growing in Turkey. Asıan J Chem. Commun., 21, 4785-4788.
  • Schütz, K., Kammerer, D.R., Carle, R., & Schieber, A. (2005). Characterization of phenolic acids and flavonoids in dandelion (Taraxacum officinale WEB. ex WIGG.) root and herb by high‐performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom.,19, 179–186. https://doi.org/10.3390/10.1002/rcm.1767
  • Simirgiotis, M.J., Benites, J., Areche, C., & Sepulveda, B. (2015). Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by HPLC-PDA-ESI-MS. Molec., 20, 11490-11507. https://doi.org/10.3390/molecules200611490
  • Tsevegsuren, N., Edrada, R., Lin, W., Ebel, R., Torre, C., Ortlepp, S., Wray, V., & Proksch, P. (2007). Biologically active natural products from Mongolian medicinal plants Scorzonera divaricate and Scorzonera pseudodivaricata. J Nat Prod., 70, 962 967. http://dx.doi.org/10.1021/np070013r
  • Turumtay, E.A., Islamoglu, F., Cavus, D., Sahin, H., & Turumtay, H. (2014). Bartel Vanholme Correlation between phenolic compounds and antioxidant activity of Anzer tea (Thymus praecox Opiz subsp. Caucasicus var. caucasicus). Ind Crop Prod., 52, 687 694. https://doi.org/10.1016/j.indcrop.2013.11.042
  • Wang, Y., Edrada-Ebel, R.A., Tsevegsuren, N., Sendker, J., Braun, M., Wray, V., Lin, W., & Proksch, P. (2009). Dihydrostilbene Derivatives from the Mongolian Medicinal Plant Scorzonera radiata. J. Nat. Prod., 72, 671–675. http://dx.doi.org/10.1021/np800782f
  • Wei, F., Jinglou, C., Yaling, C., Yongfang, L., Liming, C., Lei, P., Zhou, D., Liang, X., & Ruan, J. (2010). Antioxidant, free radical scavenging, antiinflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch.et Sav.) Ching. J. Ethnopharmacol., 130, 521–528. https://doi.org/10.1016/j.jep.2010.05.039

Polyphenolic composition and Antioxidant Effect of Aerial Parts and Roots Extracts from Scorzonera veratrifolia

Year 2021, Volume: 8 Issue: 3, 284 - 299, 10.09.2021

Duygu Taşkın Mert Geçim Ahmet Doğan Ayfer Beceren

https://doi.org/10.21448/ijsm.943707
Cited By: 4

Abstract

Antioxidant activities of the different extracts (n-heptane, chloroform, methanol) from the roots and aerial parts of Scorzonera veratrifolia by maceration method, as well as total phenolic and flavonoid content were examined first time in this study. The findings revealed that the methanol extract from S. veratrifolia aerial parts exhibited greater DPPH radical scavenging (IC50: 0.62±0.60 mg/mL) and iron (III) reduction capacity (1.56±0.03 mM Fe2+/mg extract). Furthermore, aerial parts methanol extract has the highest concentration of total phenolic (46.3±1.1 mgGAE/g extract) and flavonoid (0.013±0.002 mg QE/mg extract) compounds. Based on these findings, the main phenolic content of aerial parts methanol extract was analyzed by LC-ESI-QTOF/MS, as this extract was found to contain the strongest antioxidant as well as the highest amount of phenolics and flavonoids as compared to the others. Quinic acid, chlorogenic acid, rutin, liquiritin, quercetin hexoside, luteolin-7-O-rutinoside, and di-O-caffeoylquinic acid compounds were identified as major compounds in methanol extract. The findings showed that aerial parts of S. veratrifolia, rather than its roots, could be used as a source of antioxidants.

Keywords

Antioxidant LC-MS Scorzonera phenolic

Project Number

217S050

References

  • Acıkara, O.B., Cıtıoglu, G.S., & Ozkan, A.M.G. (2013). Qualitative and quantitative analysis of phenolic acids in Scorzonera tomentosa L. Turk J Pharm Sci., 10, 1-8.
  • Acıkara, O.B., Ergene, B., Bakar, F., Cıtoglu, G.S., & Nebioglu, S. (2017). Evaluation of Antioxidant Activities and Phenolic Compounds of Scorzonera latifolia (Fisch. & Mey.) DC. Collected from Different Geographic Origins in Turkey. Turk J Pharm Sci., 14, 179-184. http://dx.doi.org/10.4274/tjps.57070
  • Akyüz, E., Özyürek, M., Güçlü, K., & Apak, M.R. (2013). Novel pro-oxidant activity assay for polyphenols vitamins C and E using a modified CUPRAC method. Talanta., 115, 583–589. https://doi.org/10.1016/j.talanta.2013.06.006
  • Benzie, I.F., & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem., 239, 70 76. https://doi.org/10.1006/abio.1996.0292
  • Bursal, E., Taslimi, P., Goren, A.C., & Gulcin, I. (2020). Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua. Biocatal. Agric. Biotechnol., 28, 101711. https://doi.org/10.1016/j.bcab.2020.101711
  • Chedea, V.S., Braıcu, C., & Cocacıu, C. (2010). Antioxidant/prooxidant activity of a polyphenolic grape seed extract. Food Chemi., 121, 132 139. https://doi.org/10.1016/j.foodchem.2009.12.020
  • Erden, Y., Kırbag, S., & Yılmaz, O. (2013). Phytochemical Composition and Antioxidant Activity of Some Scorzonera Species. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci., 83, 271–276. http://dx.doi.org/10.1007/s40011-012-0129-7
  • Hallıwell, B. (2008). Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies?. Arch. Biochem. Biophys., 476, 107 112. https://doi.org/10.1016/j.abb.2008.01.028
  • Hofmann, T., Nebehaj, E., & Albert, L. (2016). Antioxidant properties and detailed polyphenol profiling of Europeanhornbeam (Carpinus betulus L.) leaves by multiple antioxidantcapacity assays and high performance liquid chromatography/multistage electrospray mass spectrometry. Ind Crop Prod., 87, 340 349. https://doi.org/10.1016/j.indcrop.2016.04.037
  • Huyut, Z., Beydemir, Ş., & Gülçin, İ. (2017). Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem Res Int., 2017, 10. https://doi.org/10.1155/2017/7616791
  • Karagöz, A., Artuna, F.T., Ozcan, G., Melikoglu, G., Anıl, S., Kultur, S., & Sutlupınar, N. (2015). In vitro evaluation of antioxidant activity of some plant methanol extracts. Biotechnol Biotec EQ., 29, 1184-1189. http://dx.doi.org/10.1080/13102818.2015.1080600
  • Nasseri, M.A., Bigy, S.S., Allahresani, A., & Malekaneh, M. (2015). Assessment of Antioxidant Activity, Chemical Characterization and Evaluation of Fatty Acid Compositions of Scorzonera paradoxa Fisch and C. A. Mey. Jundishapur J Nat Pharm Prod., 10, 19781. http://dx.doi.org/10.17795/jjnpp-19781
  • Papettı, A., Daglıa, M., Grısolı, P., Dacarro, C., Gregottı, C., & Gazzanı, G. (2006). Anti- and pro-oxidant activity of Cichorium genus vegetables and effect of thermal treatment in biological systems. Food Chem., 97, 157 165. https://doi.org/10.1016/j.foodchem.2005.03.036
  • Samatha, T., Shyamsundarachary, R., Srinivas, P., & Swamy, N.R. (2012). Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian J. Pharmaceut. Clin. Res., 5, 177–179.
  • Sarı, A. (2010). Two new 3-benzylphthalides from Scorzonera veratrifolia Fenzl. Nat Prod Res. 24, 56-62. http://dx.doi.org/10.1080/14786410902800699
  • Sarı, A., Ozbek, B., & Ozgokce, F. (2009). Antimicrobial activities of two Scorzonera species growing in Turkey. Asıan J Chem. Commun., 21, 4785-4788.
  • Schütz, K., Kammerer, D.R., Carle, R., & Schieber, A. (2005). Characterization of phenolic acids and flavonoids in dandelion (Taraxacum officinale WEB. ex WIGG.) root and herb by high‐performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom.,19, 179–186. https://doi.org/10.3390/10.1002/rcm.1767
  • Simirgiotis, M.J., Benites, J., Areche, C., & Sepulveda, B. (2015). Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by HPLC-PDA-ESI-MS. Molec., 20, 11490-11507. https://doi.org/10.3390/molecules200611490
  • Tsevegsuren, N., Edrada, R., Lin, W., Ebel, R., Torre, C., Ortlepp, S., Wray, V., & Proksch, P. (2007). Biologically active natural products from Mongolian medicinal plants Scorzonera divaricate and Scorzonera pseudodivaricata. J Nat Prod., 70, 962 967. http://dx.doi.org/10.1021/np070013r
  • Turumtay, E.A., Islamoglu, F., Cavus, D., Sahin, H., & Turumtay, H. (2014). Bartel Vanholme Correlation between phenolic compounds and antioxidant activity of Anzer tea (Thymus praecox Opiz subsp. Caucasicus var. caucasicus). Ind Crop Prod., 52, 687 694. https://doi.org/10.1016/j.indcrop.2013.11.042
  • Wang, Y., Edrada-Ebel, R.A., Tsevegsuren, N., Sendker, J., Braun, M., Wray, V., Lin, W., & Proksch, P. (2009). Dihydrostilbene Derivatives from the Mongolian Medicinal Plant Scorzonera radiata. J. Nat. Prod., 72, 671–675. http://dx.doi.org/10.1021/np800782f
  • Wei, F., Jinglou, C., Yaling, C., Yongfang, L., Liming, C., Lei, P., Zhou, D., Liang, X., & Ruan, J. (2010). Antioxidant, free radical scavenging, antiinflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch.et Sav.) Ching. J. Ethnopharmacol., 130, 521–528. https://doi.org/10.1016/j.jep.2010.05.039
There are 22 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Duygu Taşkın 0000-0002-5279-0900

Mert Geçim This is me 0000-0002-9575-7801

Ahmet Doğan 0000-0003-0603-5100

Ayfer Beceren 0000-0002-6770-6089

Project Number 217S050
Publication Date September 10, 2021
Submission Date May 29, 2021
Published in Issue Year 2021 Volume: 8 Issue: 3

Cite

APA Taşkın, D., Geçim, M., Doğan, A., Beceren, A. (2021). Polyphenolic composition and Antioxidant Effect of Aerial Parts and Roots Extracts from Scorzonera veratrifolia. International Journal of Secondary Metabolite, 8(3), 284-299. https://doi.org/10.21448/ijsm.943707

Cited By

Natural Antioxidant Extracts as Anti-browning Agents from Algerian Date Palm Fruit
Current Enzyme Inhibition
https://doi.org/10.2174/1573408019666230807161244
Comparative phytochemical and bioactivity studies on two related Scorzonera L. species: A chemotaxonomic contribution
Biochemical Systematics and Ecology
https://doi.org/10.1016/j.bse.2023.104743
Biological Activity Evaluation of Scorzonera tomentosa L.
Türkiye Tarımsal Araştırmalar Dergisi
https://doi.org/10.19159/tutad.1278795
The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia
International Journal of Secondary Metabolite
https://doi.org/10.21448/ijsm.1084743
International Journal of Secondary Metabolite

e-ISSN: 2148-6905