Research Article
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Determination of Multi-Elemental Analysis and Antioxidant Activities of Helichrysum arenarium (L.) Moench Species

Year 2023, Volume: 43 Issue: 2, 128 - 141, 01.06.2023

Adil Umaz Kader Umaz Fırat Aydın Işıl Aydın

https://doi.org/10.52794/hujpharm.1118558
Cited By: 1

Abstract

In this study, multi-elemental analysis and antioxidant activity of samples belonging to two different populations of Helichrysum arenarium (L.) Moench known as the immortal flower was determined. The results obtained were compared among themselves. Ca, Mg, Al, Fe, Na, Li, Be, B, Ti, Cr, Ni, Zn, Mo, and Pb element contents of the sample belonging to the B population were determined that be higher than that of the other population. The K, V, Mn, Co, Cu, As, Se, Sn, and Ba element contents of the sample belonging to the G population were determined that be higher than that of the other population. When the antioxidant activity results of the species were evaluated; according to the CUPRAC (Cupric Reducing Antioxidant Capacity) method, the sample extracts of the B and G populations were determined that showed lower activity than the standard BHA, BHT, and Trolox values at 20 and 40 µg/mL concentrations, and showed close activity compared to the standards at 80 µg/mL concentrations. The species was determined that be antioxidant activity even at low concentrations. According to DPPH (2,2-diphenyl-1-picrylhydrazyl) method, the antioxidant activity of the extract of the B and G populations was determined as 22.95 and 23.76 mg TE/mL, respectively.

Keywords

Helichrysum arenarium Antioxidant Bivariate analysis ICP-MS

References

  • Liu X, Jing X, Li G. A process to acquire essential oil by distillation concatenated liquid-liquid extraction and flavonoids by solid-liquid extraction simultaneously from Helichrysum arenarium (L.) Moench inflorescences under ionic liquid-microwave mediated. Sep Purif Technol. 2019;209(2019):164–74. https://doi.org/10.1016/j.seppur.2018.07.028
  • Czinner E, Lemberkovics É, Bihátsi-Karsai E, Vitányi G, Lelik L. Composition of the essential oil from the inflorescence of Helichrysum arenarium (L.) Moench. J Essent Oil Res. 2000;12(6):728–30. https://doi.org/10.1080/10412905.2000.9712202
  • Albayrak S, Aksoy A, Saǧdiç O, Budak Ü. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish J Biol. 2010;34(4):463–73. https://doi.org/10.3906/biy-0901-4
  • Eroǧlu HE, Hamzaoǧlu E, Budak Ü, Aksoy A, Albayrak S. Cytogenetic effects of Helichrysum arenarium in human lymphocytes cultures. Turkish J Biol. 2010;34(3):253–9. https://doi.org/10.3906/biy-0906-31
  • Figas A, Tomaszewska-Sowa M, Sawilska A, Keutgen AJ. Improvement of in vitro propagation and acclimation of Helichrysum arenarium L. Moench. Acta Sci Pol Hortorum Cultus. 2016;15(4):17–26.
  • Moghadam HD, Sani A mohamadi, Sangatash MM. Inhibitory effect of Helichrysum arenarium essential oil on the growth of food contaminated microorganisms. J Essent Oil-Bearing Plants. 2014;17(5):911–21. https://doi.org/10.1080/0972060X.2014.890073
  • Umaz A, Umaz K. İki farklı lokasyona ait altın otunun (Helichrysum arenarium) uçucu bileşenlerinin belirlenmesi ve karşılaştırılması. Gümüşhane Üniversitesi Fen Bilim Enstitüsü Derg. 2020;10(3):592–600. https://doi.org/10.17714/gumusfenbil.621772
  • Jarzycka A, Lewińska A, Gancarz R, Wilk KA. Assessment of extracts of Helichrysum arenarium, Crataegus monogyna, Sambucus nigra in photoprotective UVA and UVB; photostability in cosmetic emulsions. J Photochem Photobiol B Biol. 2013;128:50–7. https://doi.org/10.1016/j.jphotobiol.2013.07.029
  • Reidel RVB, Cioni PL, Ruffoni B, Cervelli C, Pistelli L. Aroma profile and essential oil composition of Helichrysum species. Nat Prod Commun. 2017;12(9):1507–12. https://doi.org/10.1177/1934578X1701200931
  • Kutluk I, Aslan M, Orhan IE, Özçelik B. Antibacterial, antifungal and antiviral bioactivities of selected Helichrysum species. South African J Bot. 2018;119:252–7. https://doi.org/10.1016/j.sajb.2018.09.009
  • Rančić A, Soković M, Vukojević J, Simić A, Marin P, Duletić-Laušević S, et al. Chemical composition and antimicrobial activities of essential oils of Myrrhis odorata (L.) Scop, Hypericum perforatum L. and Helichrysum arenarium (L.) Moench. J Essent Oil Res. 2005;17(3):341–5. https://doi.org/10.1080/10412905.2005.9698925
  • Kladar NV, Anačkov GT, Rat MM, Srđenović BU, Grujić NN, Šefer EI., Božin BN. Biochemical characterization of Helichrysum italicum (Roth) G . Don subsp . italicum (Asteraceae) from Montenegro : Phytochemical screening, chemotaxonomy, and antioxidant properties. Chem Biodivers. 2015;12:419–31. https://doi.org/10.1002/cbdv.201400174
  • Czinner E, Hagymási K, Blázovics A, Kéry Á, Szoke É, Lemberkovics É. In vitro antioxidant properties of Helichrysum arenarium (L.) Moench. J Ethnopharmacol. 2000;73(3):437–43. https://doi.org/10.1016/S0378-8741(00)00304-4
  • Tepe B, Sokmen M, Askin Akpulat H, Sokmen A. In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey. Food Chem. 2005;90(4):685–9. https://doi.org/10.1016/j.foodchem.2004.04.030
  • Mao Z, Gan C, Zhu J, Ma N, Wu L, Wang L, et al. Anti-atherosclerotic activities of flavonoids from the flowers of Helichrysum arenarium L. MOENCH through the pathway of anti-inflammation. Bioorganic Med Chem Lett. 2017;27(12):2812–7. http://dx.doi.org/10.1016/j.bmcl.2017.04.076
  • Gradinaru AC, Silion M, Trifan A, Miron A, Aprotosoaie AC. Helichrysum arenarium subsp. arenarium: Phenolic composition and antibacterial activity against lower respiratory tract pathogens. Nat Prod Res. 2014;28(22):2076–80. https://doi.org/10.1080/14786419.2014.924931
  • Akin M, Saki N. Antimicrobial, DPPH scavenging and tyrosinase inhibitory activities of Thymus vulgaris, Helichrysum arenarium and Rosa damascena mill. ethanol extracts by using TLC bioautography and chemical screening methods. J Liq Chromatogr Relat Technol. 2019;42(7–8):204–16. https://doi.org/10.1080/10826076.2019.1591977
  • Lv H, Zhang Y, Sun Y, Duan Y. Multielement patterns of Danshen (Salvia miltiorrhiza) from origins in China. Microchem J. 2019;145(2019):273–9. https://doi.org/10.1016/j.microc.2018.10.055
  • Yener İ. Trace element analysis in some plants species by inductively coupled plasma optical emission spectrometry (ICP-OES). Iğdır Üniversitesi Fen Bilim Enstitüsü Derg. 2019;9(3):1492–502. https://doi.org/10.21597/jist. 517739
  • Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V. Multi-element analysis of mineral and trace elements in medicinal herbs and their infusions. Food Chem. 2012;135(2):494–501. http://dx.doi.org/10.1016/j.foodchem.2012.05.002
  • Szymczycha-Madeja A, Welna M, Zyrnicki W. Multi-Element analysis, bioavailability and fractionation of herbal tea products. J Braz Chem Soc,. 2013;24(5):777–87. http://dx.doi.org/10.5935/0103-5053.20130102
  • Zhang X, Ding W, Li J, Liu F, Zhou X, Tian S. Multi-elemental analysis of Ziziphora clinopodioides from different regions, periods and parts using atomic absorption spectrometry and chemometric approaches. Brazilian J Pharmacogn. 2015;25(5):465–72. http://dx.doi.org/10.1016/j.bjp.2015.07.021
  • Targan Ş, Yelboğa EG, Cittan M. Macro and trace element contents of some wild plants consumed as vegetable in Manisa District, Turkey. J Turkish Chem Soc Sect A Chem. 2018;5(2):751–62. http://dx.doi.org/10.18596/jotcsa.363151
  • Umaz A, Aydin F, Firat M, Ertas A. Bazı geofitlerin indüktif eşleşmiş plazmalı kütle spektrometresi (ICP - MS) ile makro ve mikro element analizi. Dicle Üniversitesi Fen Bilim Enstitüsü Derg. 2021;10(1):47–58.
  • Apak R, Güçlü K, Demirata B, Özyürek M, Çelik SE, Bektaşoǧlu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. J bra. 2007;12(7):1496–547. http://dx.doi.org/10.3390/12071496
  • Makhlouf-gafsi I, Krichen F, Mansour R Ben, Mokni A, Bougatef A, Blecher C, et al. Ultrafiltration and thermal processing effects on Maillard reaction products and biological properties of date palm sap syrups (Phoenix dactylifera L.). Food Chem. 2018;256(1):397–404. https://doi.org/10.1016/j.foodchem.2018.02.145
  • Lemberkovics É, Czinner E, Szentmihályi K, Balázs A, Szoke É. Comparative evaluation of helichrysi flos herbal extracts as dietary sources of plant polyphenols, and macro- and microelements. Food Chem. 2002;78(1):119–27. https://doi.org/10.1016/S0308-8146(02)00204-2
  • Vural A. Gold and silver content of plant Helichrysum Arenarium, popularly known as the golden flower, growing in Gümüşhane, NE Turkey. Acta Phys Pol A. 2017;132(3):978–80.
  • Vural A. Relationship between the geological environment and element accumulation capacity of Helichrysum arenarium. Arab J Geosci 2018;11(11):258. https://doi.org/10.1007/s12517-018-3609-0
  • Stankov S, Fidan H, Petkova N. Phytochemical composition of Нelichrysum arenarium (L.) Moench essential oil (aerial parts) from Turkey. Ukrainian Food J. 2020;9(3):503–512. https://doi.org/10.24263/2304-974X-2020-9-3-3
  • Kulisic-Bilusic T, Katalinic V, Dragovic-Uzelac V, Ljubenkov I, Krisko A, Dejanovic B, et al. Antioxidant and acetylcholinesterase inhibiting activity of several aqueous tea infusions in vitro. Food Technol Biotechnol. 2008;46(4):368–75.
  • Kramberger K, Pražnikar ZJ, Arbeiter AB, Petelin A, Bandelj D, Kenig S. A comparative study of the antioxidative effects of Helichrysum italicum and Helichrysum arenarium infusions. Antioxidants. 2021;10(1):380–95. https://doi.org/10.3390/antiox10030380
  • Babotă M, Mocan A, Vlase L, Crisan O, Ielciu I, Gheldiu AM, et al. Phytochemical analysis, antioxidant and antimicrobial activities of Helichrysum arenarium (L.) moench. and Antennaria dioica (L.) gaertn. flowers. Molecules. 2018;23(2):409–24. https://doi.org/10.3390/molecules23020409
  • Judzentiene A, Budiene J, Nedveckyte I, Garjonyte R. Antioxidant and toxic activity of Helichrysum arenarium (L.) Moench and Helichrysum italicum (Roth) G. Don essential oils and extracts. Molecules. 2022;27:1311–30. https://doi.org/10.3390/molecules27041311

Determination of Multi-Elemental Analysis and Antioxidant Activities of Helichrysum arenarium (L.) Moench Species

Year 2023, Volume: 43 Issue: 2, 128 - 141, 01.06.2023

Adil Umaz Kader Umaz Fırat Aydın Işıl Aydın

https://doi.org/10.52794/hujpharm.1118558
Cited By: 1

Abstract

In this study, multi-elemental analysis and antioxidant activity of samples belonging to two different populations of Helichrysum arenarium (L.) Moench known as the immortal flower was determined. The results obtained were compared among themselves. Ca, Mg, Al, Fe, Na, Li, Be, B, Ti, Cr, Ni, Zn, Mo, and Pb element contents of the sample belonging to the B population were determined that be higher than that of the other population. The K, V, Mn, Co, Cu, As, Se, Sn, and Ba element contents of the sample belonging to the G population were determined that be higher than that of the other population. When the antioxidant activity results of the species were evaluated; according to the CUPRAC (Cupric Reducing Antioxidant Capacity) method, the sample extracts of the B and G populations were determined that showed lower activity than the standard BHA, BHT, and Trolox values at 20 and 40 µg/mL concentrations, and showed close activity compared to the standards at 80 µg/mL concentrations. The species was determined that be antioxidant activity even at low concentrations. According to DPPH (2,2-diphenyl-1-picrylhydrazyl) method, the antioxidant activity of the extract of the B and G populations was determined as 22.95 and 23.76 mg TE/mL, respectively.

Keywords

Helichrysum arenarium Antioxidant Bivariate analysis ICP-MS

References

  • Liu X, Jing X, Li G. A process to acquire essential oil by distillation concatenated liquid-liquid extraction and flavonoids by solid-liquid extraction simultaneously from Helichrysum arenarium (L.) Moench inflorescences under ionic liquid-microwave mediated. Sep Purif Technol. 2019;209(2019):164–74. https://doi.org/10.1016/j.seppur.2018.07.028
  • Czinner E, Lemberkovics É, Bihátsi-Karsai E, Vitányi G, Lelik L. Composition of the essential oil from the inflorescence of Helichrysum arenarium (L.) Moench. J Essent Oil Res. 2000;12(6):728–30. https://doi.org/10.1080/10412905.2000.9712202
  • Albayrak S, Aksoy A, Saǧdiç O, Budak Ü. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish J Biol. 2010;34(4):463–73. https://doi.org/10.3906/biy-0901-4
  • Eroǧlu HE, Hamzaoǧlu E, Budak Ü, Aksoy A, Albayrak S. Cytogenetic effects of Helichrysum arenarium in human lymphocytes cultures. Turkish J Biol. 2010;34(3):253–9. https://doi.org/10.3906/biy-0906-31
  • Figas A, Tomaszewska-Sowa M, Sawilska A, Keutgen AJ. Improvement of in vitro propagation and acclimation of Helichrysum arenarium L. Moench. Acta Sci Pol Hortorum Cultus. 2016;15(4):17–26.
  • Moghadam HD, Sani A mohamadi, Sangatash MM. Inhibitory effect of Helichrysum arenarium essential oil on the growth of food contaminated microorganisms. J Essent Oil-Bearing Plants. 2014;17(5):911–21. https://doi.org/10.1080/0972060X.2014.890073
  • Umaz A, Umaz K. İki farklı lokasyona ait altın otunun (Helichrysum arenarium) uçucu bileşenlerinin belirlenmesi ve karşılaştırılması. Gümüşhane Üniversitesi Fen Bilim Enstitüsü Derg. 2020;10(3):592–600. https://doi.org/10.17714/gumusfenbil.621772
  • Jarzycka A, Lewińska A, Gancarz R, Wilk KA. Assessment of extracts of Helichrysum arenarium, Crataegus monogyna, Sambucus nigra in photoprotective UVA and UVB; photostability in cosmetic emulsions. J Photochem Photobiol B Biol. 2013;128:50–7. https://doi.org/10.1016/j.jphotobiol.2013.07.029
  • Reidel RVB, Cioni PL, Ruffoni B, Cervelli C, Pistelli L. Aroma profile and essential oil composition of Helichrysum species. Nat Prod Commun. 2017;12(9):1507–12. https://doi.org/10.1177/1934578X1701200931
  • Kutluk I, Aslan M, Orhan IE, Özçelik B. Antibacterial, antifungal and antiviral bioactivities of selected Helichrysum species. South African J Bot. 2018;119:252–7. https://doi.org/10.1016/j.sajb.2018.09.009
  • Rančić A, Soković M, Vukojević J, Simić A, Marin P, Duletić-Laušević S, et al. Chemical composition and antimicrobial activities of essential oils of Myrrhis odorata (L.) Scop, Hypericum perforatum L. and Helichrysum arenarium (L.) Moench. J Essent Oil Res. 2005;17(3):341–5. https://doi.org/10.1080/10412905.2005.9698925
  • Kladar NV, Anačkov GT, Rat MM, Srđenović BU, Grujić NN, Šefer EI., Božin BN. Biochemical characterization of Helichrysum italicum (Roth) G . Don subsp . italicum (Asteraceae) from Montenegro : Phytochemical screening, chemotaxonomy, and antioxidant properties. Chem Biodivers. 2015;12:419–31. https://doi.org/10.1002/cbdv.201400174
  • Czinner E, Hagymási K, Blázovics A, Kéry Á, Szoke É, Lemberkovics É. In vitro antioxidant properties of Helichrysum arenarium (L.) Moench. J Ethnopharmacol. 2000;73(3):437–43. https://doi.org/10.1016/S0378-8741(00)00304-4
  • Tepe B, Sokmen M, Askin Akpulat H, Sokmen A. In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey. Food Chem. 2005;90(4):685–9. https://doi.org/10.1016/j.foodchem.2004.04.030
  • Mao Z, Gan C, Zhu J, Ma N, Wu L, Wang L, et al. Anti-atherosclerotic activities of flavonoids from the flowers of Helichrysum arenarium L. MOENCH through the pathway of anti-inflammation. Bioorganic Med Chem Lett. 2017;27(12):2812–7. http://dx.doi.org/10.1016/j.bmcl.2017.04.076
  • Gradinaru AC, Silion M, Trifan A, Miron A, Aprotosoaie AC. Helichrysum arenarium subsp. arenarium: Phenolic composition and antibacterial activity against lower respiratory tract pathogens. Nat Prod Res. 2014;28(22):2076–80. https://doi.org/10.1080/14786419.2014.924931
  • Akin M, Saki N. Antimicrobial, DPPH scavenging and tyrosinase inhibitory activities of Thymus vulgaris, Helichrysum arenarium and Rosa damascena mill. ethanol extracts by using TLC bioautography and chemical screening methods. J Liq Chromatogr Relat Technol. 2019;42(7–8):204–16. https://doi.org/10.1080/10826076.2019.1591977
  • Lv H, Zhang Y, Sun Y, Duan Y. Multielement patterns of Danshen (Salvia miltiorrhiza) from origins in China. Microchem J. 2019;145(2019):273–9. https://doi.org/10.1016/j.microc.2018.10.055
  • Yener İ. Trace element analysis in some plants species by inductively coupled plasma optical emission spectrometry (ICP-OES). Iğdır Üniversitesi Fen Bilim Enstitüsü Derg. 2019;9(3):1492–502. https://doi.org/10.21597/jist. 517739
  • Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V. Multi-element analysis of mineral and trace elements in medicinal herbs and their infusions. Food Chem. 2012;135(2):494–501. http://dx.doi.org/10.1016/j.foodchem.2012.05.002
  • Szymczycha-Madeja A, Welna M, Zyrnicki W. Multi-Element analysis, bioavailability and fractionation of herbal tea products. J Braz Chem Soc,. 2013;24(5):777–87. http://dx.doi.org/10.5935/0103-5053.20130102
  • Zhang X, Ding W, Li J, Liu F, Zhou X, Tian S. Multi-elemental analysis of Ziziphora clinopodioides from different regions, periods and parts using atomic absorption spectrometry and chemometric approaches. Brazilian J Pharmacogn. 2015;25(5):465–72. http://dx.doi.org/10.1016/j.bjp.2015.07.021
  • Targan Ş, Yelboğa EG, Cittan M. Macro and trace element contents of some wild plants consumed as vegetable in Manisa District, Turkey. J Turkish Chem Soc Sect A Chem. 2018;5(2):751–62. http://dx.doi.org/10.18596/jotcsa.363151
  • Umaz A, Aydin F, Firat M, Ertas A. Bazı geofitlerin indüktif eşleşmiş plazmalı kütle spektrometresi (ICP - MS) ile makro ve mikro element analizi. Dicle Üniversitesi Fen Bilim Enstitüsü Derg. 2021;10(1):47–58.
  • Apak R, Güçlü K, Demirata B, Özyürek M, Çelik SE, Bektaşoǧlu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. J bra. 2007;12(7):1496–547. http://dx.doi.org/10.3390/12071496
  • Makhlouf-gafsi I, Krichen F, Mansour R Ben, Mokni A, Bougatef A, Blecher C, et al. Ultrafiltration and thermal processing effects on Maillard reaction products and biological properties of date palm sap syrups (Phoenix dactylifera L.). Food Chem. 2018;256(1):397–404. https://doi.org/10.1016/j.foodchem.2018.02.145
  • Lemberkovics É, Czinner E, Szentmihályi K, Balázs A, Szoke É. Comparative evaluation of helichrysi flos herbal extracts as dietary sources of plant polyphenols, and macro- and microelements. Food Chem. 2002;78(1):119–27. https://doi.org/10.1016/S0308-8146(02)00204-2
  • Vural A. Gold and silver content of plant Helichrysum Arenarium, popularly known as the golden flower, growing in Gümüşhane, NE Turkey. Acta Phys Pol A. 2017;132(3):978–80.
  • Vural A. Relationship between the geological environment and element accumulation capacity of Helichrysum arenarium. Arab J Geosci 2018;11(11):258. https://doi.org/10.1007/s12517-018-3609-0
  • Stankov S, Fidan H, Petkova N. Phytochemical composition of Нelichrysum arenarium (L.) Moench essential oil (aerial parts) from Turkey. Ukrainian Food J. 2020;9(3):503–512. https://doi.org/10.24263/2304-974X-2020-9-3-3
  • Kulisic-Bilusic T, Katalinic V, Dragovic-Uzelac V, Ljubenkov I, Krisko A, Dejanovic B, et al. Antioxidant and acetylcholinesterase inhibiting activity of several aqueous tea infusions in vitro. Food Technol Biotechnol. 2008;46(4):368–75.
  • Kramberger K, Pražnikar ZJ, Arbeiter AB, Petelin A, Bandelj D, Kenig S. A comparative study of the antioxidative effects of Helichrysum italicum and Helichrysum arenarium infusions. Antioxidants. 2021;10(1):380–95. https://doi.org/10.3390/antiox10030380
  • Babotă M, Mocan A, Vlase L, Crisan O, Ielciu I, Gheldiu AM, et al. Phytochemical analysis, antioxidant and antimicrobial activities of Helichrysum arenarium (L.) moench. and Antennaria dioica (L.) gaertn. flowers. Molecules. 2018;23(2):409–24. https://doi.org/10.3390/molecules23020409
  • Judzentiene A, Budiene J, Nedveckyte I, Garjonyte R. Antioxidant and toxic activity of Helichrysum arenarium (L.) Moench and Helichrysum italicum (Roth) G. Don essential oils and extracts. Molecules. 2022;27:1311–30. https://doi.org/10.3390/molecules27041311
There are 34 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences, Analytical Chemistry
Journal Section Research Articles
Authors

Adil Umaz 0000-0003-2438-5454

Kader Umaz

Fırat Aydın 0000-0002-0868-2769

Işıl Aydın 0000-0001-6571-6032

Publication Date June 1, 2023
Acceptance Date September 22, 2022
Published in Issue Year 2023 Volume: 43 Issue: 2

Cite

Vancouver Umaz A, Umaz K, Aydın F, Aydın I. Determination of Multi-Elemental Analysis and Antioxidant Activities of Helichrysum arenarium (L.) Moench Species. HUJPHARM. 2023;43(2):128-41.

Cited By

Molehiya (Corchorus olitorius L.) ve Altın otu (Helichrysum arenarium L.) ekstraktlarının fitokimyasal içerikleri, antioksidan ve antibakteriyel özellikleri ile fenolik bileşenlerinin belirlenmesi
Harran Tarım ve Gıda Bilimleri Dergisi
https://doi.org/10.29050/harranziraat.1327041