Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?

Sertaç Argun Kıvanç; Berna Akova; Merih Kıvanç

doi:10.26650/experimed.1172749

Research Article

Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?

Year 2022, Volume: 12 Issue: 3, 103 - 107, 31.12.2022

Sertaç Argun Kıvanç Berna Akova Merih Kıvanç

https://doi.org/10.26650/experimed.1172749

Abstract

Objective: Our aim was to investigate the formation of Staphylococcus (S.) epidermidis biofilm on hydrophobic acrylic lenses and whether the inhibition of the formed biofilm is possible with probiotic Lactobacillus (L.) rhamnosus 312 and parabiotic prepared from it.

Materials and Methods: The probiotic bacteria L. rhamnosus 312 and intercellular adhesion (ICA) gene-positive tested bacteria S. epidermidis KA15.8 were used in the study from stock. To obtain the parabiotic the cultures were developed in De Man Rogosa and Sharpe (MRS) broth for 48 hours and autoclaved at 121ºC for 15 minutes. Biofilms on hydrophobic acrylic intraocular lenses and the antibiofilm effects of parabiotic and probiotic L. rhamnosus were evaluated. Scanning electron microscopy photos of biofilms produced on intraocular lenses (IOLs) were taken.

Results: Probiotic L. rhamnosus 312 and the parabiotic test showed antibacterial activity on test bacteria, ICA positive S. epidermidis KA15.8. However, the probiotic L. rhamnosus 312 zone diameter was found to be wider. After the biofilm was formed, the addition of parabiotic inhibited the biofilm formed by S. epidermis KA15.8 by 58.29%. The number of S. epidermidis KA15.8 in the biofilm also decreased.

Conclusion: Parabiotic and probiotic L. rhamnosus 312 was found effective for its antibiofilm effect. However, further studies with different concentrations are needed

Keywords

Probiotic, parabiotic, ophthalmology, cataract, intraocular lens, biofilm

Supporting Institution

Anadolu University

Project Number

1404F202

Thanks

We would like to thank Associate Professor XXXX for his contribution in taking SEM photographs in the study.

References

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22. Mazoteras P, Quiles MG, Martins Bispo PJ, Höfling-Lima AL, Pignatari AC, Casaroli-Marano RP. Analysis of intraocular lens biofilms and fluids after long-term uncomplicated cataract surgery. Am J Ophthalmol 2016; 169: 46-57. [CrossRef] google scholar
23. Kıvanç SA, Kıvanç M, Bayramlar H. Microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. J Wound Care 2016; 25: 14-9. [CrossRef] google scholar
24. El-Ganiny AM, Shaker GH, Aboelazm AA, El-Dash HA. Prevention of bacterial biofilm formation on soft contact lenses using natural compounds. J Ophthalmic Inflamm Infect 2017; 7: 11. [CrossRef] google scholar
25. Mantziari A, Salminen S, Szajewska H, Malagon-Rojas JN. Postbiotics against pathogens commonly involved in pediatric infectious diseases. Microorganisms 2020; 8: 1510. [CrossRef] google scholar
26. Rescigno M, Penna, G. Postbiotic-based composition for treatment of ocular inflammation. Patent. 2019. Avaible from: URL: WO2018024833A1.pdf (storage.googleapis.com) google scholar
27. Leshem R, Maharshak I, Ben Jacob E, Ofek I, Kremer I. The effect of nondialyzable material (NDM) cranberry extract on formation of contact lens biofilm by Staphylococcus epidermidis. Invest Ophthalmol Vis Sci 2011; 52: 4929-34. [CrossRef] google scholar
28. Szczotka-Flynn LB, Imamura Y, Chandra J, Yu C, Mukherjee PK, Pearlman E, et al. Increased resistance of contact lens-related bacterial biofilms to antimicrobial activity of soft contact lens care solutions. Cornea 2009; 28: 918-26. [CrossRef] google scholar
29. Kilvington S, Lonnen J. A comparison of regimen methods for the removal and inactivation of bacteria, fungi and Acanthamoeba from two types of silicone hydrogel lenses. Cont Lens Anterior Eye 2009; 32: 73-7. [CrossRef] google scholar
30. Tsilingiri K, Rescigno M. Postbiotics: What else? Benef Microbes 2013; 4: 101-7. [CrossRef] google scholar

Year 2022, Volume: 12 Issue: 3, 103 - 107, 31.12.2022

Sertaç Argun Kıvanç Berna Akova Merih Kıvanç

https://doi.org/10.26650/experimed.1172749

Abstract

Project Number

1404F202

References

1. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982; 37: 318-26. [CrossRef] google scholar
2. Peters G, Locci R, Pulverer G. Adherence and growth of coagulasenegative staphylococci on surfaces of intravenous catheters. J Infect Dis 1982; 146: 479-82. [CrossRef] google scholar
3. Kattan HM, Flynn HW Jr, Pflugfelder SC, Robertson C, Forster RK. Nosocomial endophthalmitis survey: current incidence of infection after intraocular surgery. Ophthalmology 1991; 98: 22738. [CrossRef] google scholar
4. Wong TY, Chee SP. The epidemiology of acute endophthalmitis after cataract surgery in an Asian population. Ophthalmology 2004; 111: 699-705. [CrossRef] google scholar
5. Miller JJ, Scott IU, Flynn HW Jr, Smiddy WE, Newton J, Miller D. Acute-onset endophthalmitis after cataract surgery (2000-2004): Incidence, clinical settings, and visual acuity outcomes after treatment. Am J Ophthalmol 2005; 139: 983-7. [CrossRef] google scholar
6. Aaberg TM Jr, Flynn HW Jr, Schiffman J, Newton J. Nosocomial acute-onset postoperative endophthalmitis survey. A 10-year review of incidence and outcomes. Ophthalmology 1998; 105: 1004-10. [CrossRef] google scholar
7. Griffiths PG, Elliot TSJ, McTaggart L. Adherence of Staphylococcus epidermidis to intraocular lenses. Br J Ophthalmol 1989; 73: 402- 6. [CrossRef] google scholar
8. Kıvanç SA, Kıvanç M, Kılıç V, Güllülü G, Özmen AT. Comparison of biofilm formation capacities of two clinical ısolates of Staphylococcus epidermidis with and without icaA and icaD genes on ıntraocular lenses. Turk J Ophthalmol 2017; 47: 68-73. [CrossRef] google scholar
9. Zegans ME, Becker HI, Budzik J, O'Toole G. The role of bacterial biofilms in ocular infections. DNA Cell Biol 2002; 21: 415-20. [CrossRef] google scholar
10. Pinna A, Sechi LA, Zanetti S, Delogu D, Carta F. Adherence of ocular isolates of Staphylococcus epidermidis to AcrySof intraocular lenses: a scanning electron microscopy and molecular biology study. Ophthalmology 2000; 107: 2162-6. [CrossRef] google scholar
11. Kodjikian L, Burillon C, Roques C, Pellon G, Freney J, Renaud FN. Bacterial adherence of Staphylococcus epidermidis to intraocular lenses: a bioluminescence and scanning electron microscopy study. Invest Ophthalmol Vis Sci 2003; 44: 4382-7. [CrossRef] google scholar
12. McKenney D, Hubner J, Muller E, Wang Y, Goldmann DA, Pier GB. The ica locus of Staphylococcus epidermidis encodes production of the capsular polysaccharide/adhesion. Infect Immun 1998; 66: 4711-20. [CrossRef] google scholar
13. Cramton SE, Gerke C, Schnell NF, Nichols WW, Gotz F. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun 1999; 67: 5427-33. [CrossRef] google scholar
14. von Eiff C, Heilmann C, Peters G. New aspects in the molecular basis of polymer-associated infections due to staphylococci. Eur J Clin Microbiol Infect Dis 1999; 18: 843-6. [CrossRef] google scholar
15. Cuevas-Gonzalez PF, Liceaga AM, Aguilar-Toala JE. Postbiotics and paraprobiotics: From concepts to applications. Food Res Int 2020; 136: 109502. [CrossRef] google scholar
16. Aguilar-Toala JE, Garcia-Varela R, Garcia HS, Mata-Haro V, Gonzalez Cordova AF,Vallejo-Cordoba B, et al. Postbiotics:an evolving term within the functional foods field. Trends Food Sci Technol 2018; 75: 105-14. [CrossRef] google scholar
17. Okajima Y, Kobayakawa S, Tsuji A, Tochikubo T. Biofilm formation by Staphylococcus epidermidis on intraocular lens material. Invest Ophthalmol Vis Sci 2006; 47: 2971-5. [CrossRef] google scholar
18. Moradi R, Molaei R, Guimaraes JT. A review on preparation and chemical analysis of postbiotics from lactic acid bacteria. Enzyme Microb Technol 2021; 143: 109722. [CrossRef] google scholar
19. Mohamed S, Elmohamady MN, Abdelrahman S, Amer MM, Abdelhamid AG. Antibacterial effects of antibiotics and cell-free preparations of probiotics against Staphylococcus aureus and Staphylococcus epidermidis associated with conjunctivitis. Saudi Pharm J 2020; 28: 1558-65. [CrossRef] google scholar
20. Hor YY, Liong MT. Use of extracellular extracts of lactic acid bacteria and bifidobacteria for the inhibition of dermatological pathogen Staphylococcus aureus. Dermatol Sin 2014; 32, 141-7. [CrossRef] google scholar
21. Negi YK, Pandey C, Saxena N, Sharma S, Garg FC, Garg SK. Isolation of antibacterial protein from Lactobacillus spp. and preparation of probiotic curd. J Food Sci Technol 2018; 55: 2011-20. [CrossRef] google scholar
22. Mazoteras P, Quiles MG, Martins Bispo PJ, Höfling-Lima AL, Pignatari AC, Casaroli-Marano RP. Analysis of intraocular lens biofilms and fluids after long-term uncomplicated cataract surgery. Am J Ophthalmol 2016; 169: 46-57. [CrossRef] google scholar
23. Kıvanç SA, Kıvanç M, Bayramlar H. Microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. J Wound Care 2016; 25: 14-9. [CrossRef] google scholar
24. El-Ganiny AM, Shaker GH, Aboelazm AA, El-Dash HA. Prevention of bacterial biofilm formation on soft contact lenses using natural compounds. J Ophthalmic Inflamm Infect 2017; 7: 11. [CrossRef] google scholar
25. Mantziari A, Salminen S, Szajewska H, Malagon-Rojas JN. Postbiotics against pathogens commonly involved in pediatric infectious diseases. Microorganisms 2020; 8: 1510. [CrossRef] google scholar
26. Rescigno M, Penna, G. Postbiotic-based composition for treatment of ocular inflammation. Patent. 2019. Avaible from: URL: WO2018024833A1.pdf (storage.googleapis.com) google scholar
27. Leshem R, Maharshak I, Ben Jacob E, Ofek I, Kremer I. The effect of nondialyzable material (NDM) cranberry extract on formation of contact lens biofilm by Staphylococcus epidermidis. Invest Ophthalmol Vis Sci 2011; 52: 4929-34. [CrossRef] google scholar
28. Szczotka-Flynn LB, Imamura Y, Chandra J, Yu C, Mukherjee PK, Pearlman E, et al. Increased resistance of contact lens-related bacterial biofilms to antimicrobial activity of soft contact lens care solutions. Cornea 2009; 28: 918-26. [CrossRef] google scholar
29. Kilvington S, Lonnen J. A comparison of regimen methods for the removal and inactivation of bacteria, fungi and Acanthamoeba from two types of silicone hydrogel lenses. Cont Lens Anterior Eye 2009; 32: 73-7. [CrossRef] google scholar
30. Tsilingiri K, Rescigno M. Postbiotics: What else? Benef Microbes 2013; 4: 101-7. [CrossRef] google scholar

There are 30 citations in total.

Details

Primary Language	English
Subjects	Clinical Sciences
Journal Section	Research Article
Authors	Sertaç Argun Kıvanç 0000-0002-0932-6977 Berna Akova 0000-0003-0995-5260 Merih Kıvanç 0000-0002-8647-3428
Project Number	1404F202
Publication Date	December 31, 2022
Submission Date	September 8, 2022
Published in Issue	Year 2022 Volume: 12 Issue: 3

Cite

Vancouver	Kıvanç SA, Akova B, Kıvanç M. Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?. Experimed. 2022;12(3):103-7.

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