Abstract
Objective: In traditional medicine, the Pulicaria species are used for various disorders such as inflamed wounds, skin diseases, and bronchitis. This study investigated the antibacterial effect of five Pulicaria species in Turkey; (Pulicaria (P) arabica (L.) Cass., P. dysen-terica (L.) Bernh., P. odora (L.) Reichb., P. sicula (L.) Moris, P. vulgaris (L.) Gaertn.) against certain significant pathogenic gram-negative and gram-positive reference bacteria.
Material and Method: Four extracts (decoction, infusion, aque-ous, and ethanol (EtOH) extracts) were prepared from the Pulicariaspecies. The antimicrobial activity of the samples was examined against reference organisms; Bacillus (B) subtilis, Enterococcus fae-calis, Escherichia coli, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus (S) aureus (MRSA), Proteus mirabilis, S. aureus andPseudomonas aeruginosa. The minimal bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) ranges of the extract samples were demonstrated based on a microbroth dilu-tion method.
Results: The EtOH extracts of the studied four Pulicaria species were found to be weakly active against gram-positive bacteria such as B. subtilis, MRSA and S. aureus but the EtOH extract of P. dy-senterica showed exceptionally good activity against the reference strains of S. aureus, MRSA. No antimicrobial activity was detected in the infusion, decoction, and aqueous extracts.
Conclusion: The Pulicaria species, especially P. dysenterica could be evaluated as antimicrobial agents. Further studies with the extracts and essential oils from Pulicaria sp. on other bacteria and pathogenic fungi should be performed.
Keywords
Supporting Institution
This study was funded by the Scientific Research Projects Coordination Unit of Istanbul University.
Project Number
Project number: 29555.
Thanks
The authors thank Scientific Research Projects Coordination Unit of Istanbul University and DÜAGEM (Altınbaş University Natural Products Research and Development Center).
References
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- 26. Touati N, Saidani K, Boudries H, Hammiche H, Ouazene N, Bedjou F. Antibacterial activity of phenolic compounds of Pulicaria odora, wild plant in northern Algeria. Int Food Res J 2018; 25(5): 2021-30. google scholar
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- 28. Zhanzhaxina AS, Seiilgazy M, Jalmakhanbetova RI, Ishmurato-va MY, Seilkhanov TM, Oyama M, et al. Flavonoids from Pulicaria vulgaris and their antimicrobial activity. Chem Nat Compd 2020; 56(5): 915-7. [CrossRef] google scholar
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- 32. Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 2005; 29(1): 41-7. google scholar
Abstract
Amaç: Geleneksel tıpta Pulicaria türleri iltihaplı yaralar, cilt hastalık-ları, bronşit gibi çeşitli rahatsızlıklarda kullanılmaktadır. Bu çalışma-da Türkiye'deki beş Pulicaria türünün (Pulicaria (P) arabica (L.) Cass., P. dysenterica (L.) Bernh., P. odora (L.) Reichb., P. sicula (L.) Moris, P. vul-garis (L.) Gaertn.), bazı önemli patojenik gram-negatif ve gram-po-zitif referans bakterilere karşı etkisi araştırılmıştır.
Gereç ve Yöntem:Pulicaria türlerinden dört ekstre (kaynatma, in-füzyon, sulu ve etanol (EtOH) ekstreleri) hazırlandı. Örneklerin, anti-mikrobiyal aktivitesi referans organizmalara karşı incelendi; Bacillus (B) subtilis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumo-niae, Metisiline dirençli Staphylococcus (S) aureus (MRSA), Proteus mirabilis, S. aureus ve Pseudomonas aeruginosa. Ekstre numuneleri-nin minimum bakterisidal konsantrasyon (MBC) ve minimum inhi-bitör konsantrasyon (MIC ) aralıkları, mikrobroth seyreltme yöntemi temel alınarak gösterilmiştir.
Bulgular: İncelenen dört Pulicaria türünün EtOH ekstrelerinin; B. subtilis, MRSA ve S. aureus gibi gram pozitif bakterilere karşı genel-likle zayıf aktif olduğu bulunmuştur, ancak P. dysenterica'nın EtOH ekstresi, S. aureus, MRSA referans suşlarına karşı çok iyi aktivite gös-termiştir. İnfüzyon, dekoksiyon ve sulu ekstrelerde antimikrobiyal aktivite tespit edilememiştir.
Sonuç:Pulicaria türleri, özellikle P. dysenterica antimikrobiyal ajan olarak değerlendirilebilir. Pulicaria sp.'den elde edilen ekstreler ve uçucu yağlar ile diğer bakteri ve bazı patojenik mantarlar üzerinde daha ileri çalışmalar yapılmalıdır.
Keywords
Project Number
Project number: 29555.
References
- 1. The Plant List. 2010; Version 1. Available from: URL: http://www. theplantlist.org/ google scholar
- 2. Tuzlacı E. Türkiye Bitkileri Geleneksel İlaç Rehberi. İstanbul: İstanbul Tıp Kitabevleri; 2016. google scholar
- 3. Al-Fatimi M. Ethnobotanical survey of medicinal plants in central Abyan governorate, Yemen. J Ethnopharmacol 2019; 241: 111973. [CrossRef] google scholar
- 4. Gonzalez-Tejero MR, Casares-Porcel M, Sanchez-Rojas CP, Ramiro-Gutierrez JM, Molero-Mesa J, Pieroni A, et al. Medicinal plants in the Mediterranean area: Synthesis of the results of the project Rubia. J Ethnopharmacol 2008; 116: 341-57. [CrossRef] google scholar
- 5. Vazquez FM, Suarez MA, Perez A. Medicinal plants used in the Barros Area, Badajoz Province (Spain). J Ethnopharmacol 1997; 55: 81-5. [CrossRef] google scholar
- 6. Yebouk C, Redouan FZ, Benı'tez G, Bouhbal M, Kadiri M, Boume-diana AI, et al. Ethnobotanical study of medicinal plants in the Adrar Province, Mauritania. J Ethnopharmacol 2020; 246: 112217. [CrossRef] google scholar
- 7. Viegi L, Pieroni A, Guarrera PM, Vangelisti R. A review of plants used in folk veterinary medicine in Italy as basis for a databank. J Ethnopharmacol 2003; 89: 221-44. [CrossRef] google scholar
- 8. Liu L, Yang J, Shi Y. Phytochemicals and biological activities of Pu-licaria species. Chem Biodivers 2010; 7(2): 327-49. [CrossRef] google scholar
- 9. Mohamed EAA, Muddathir AM, Osman MA. Antimicrobial activi-ty, phytochemical screening of crude extracts, and essential oils constituents of two Pulicaria spp. growing in Sudan. Sci Rep 2020; 10(1): 1-8. [CrossRef] google scholar
- 10. Hanbali FE, Akssira M, Ezoubeiri A, Mellouki F, Benherraf A, Blazquez AM, et al. Chemical composition and antibacterial ac-tivity of essential oil of Pulicaria odora L. J Ethnopharmacol 2005; 99(3): 399-401. [CrossRef] google scholar
- 11. Ali NAA, Sharopov FS, Alhaj M, Hill GM, Porzel A, Arnold N, et al. Chemical composition and biological activity of essential oil from Pulicaria undulata from Yemen. Nat Prod Commun 2012; 7(2): 25760. [CrossRef] google scholar
- 12. Fawzy GA, Al Ati HY, El Gamal AA. Chemical composition and bi-ological evaluation of essential oils of Pulicaria jaubertii. Pharma-cogn Mag 2013; 9(33): 28-32. [CrossRef] google scholar
- 13. Asghari G, Zahabi F, Eskandarian A, Yousefi H, Asghari M. Chemical composition and leishmanicidal activity of Pulicaria gnaphalodes essential oil. Res J Pharmacogn 2014; 1(4): 27-33. google scholar
- 14. Sharifi-Rad J, Miri A, Hoseini-Alfatemi SM, Sharifi-Rad M, Setzer WN, Hadjiakhoondi A. Chemical composition and biological activ-ity of Pulicaria vulgaris essential oil from Iran. Nat Prod Commun 2014; 9(11): 1633-6. [CrossRef] google scholar
- 15. Chaib F, Allali H, Bennaceur M, Flamini G. Chemical composition and antimicrobial activity of essential oils from the aerial parts of Asteriscus graveolens (Forssk.) Less. and Pulicaria incisa (Lam.) DC.: Two Asteraceae herbs growing wild in the Hoggar. Chem Bio-divers 2017; 14(8): e1700092. [CrossRef] google scholar
- 16. Zardi-Bergaoui A, Jelassi A, Daami-Remadi M, Harzallah-Skhiri F, Flamini G, Ascrizzi R, et al. Chemical composition and bioactivities of essential oils from Pulicaria vulgaris subsp. dentata (Sm.) Batt. growing in Tunisia. J Essent Oil Res 2020; 32(2): 111-20. [CrossRef] google scholar
- 17. Triana J, Lopez M, Perez FJ, Gonzalez-Platas J, Quintana J, Estevez F, et al. Sesquiterpenoids from Pulicaria canariensis and their cyto-toxic activities. J Nat Prod 2005; 68(4): 523-31. [CrossRef] google scholar
- 18. Yusufoglu HS. Analgesic, antipyretic, anti-inflammatory, hepato-protective and nephritic effects of the aerial parts of Pulicaria ar-abica (Family: Compositae) on rats. Asian Pac J Trop Med 2014; 7: S583-S590. [CrossRef] google scholar
- 19. Hussien TA, El-Toumy S, Hassan H, Hetta M. Cytotoxic and antioxi-dant activities of secondary metabolites from Pulicaria undulata. Int J Pharm Pharm Sci 2016; 8(9): 150-5. [CrossRef] google scholar
- 20. Ravandeh M, Valizadeh J, Noroozifar M, Khorasani-Motlagh M. Screening of chemical composition of essential oil, mineral ele-ments and antioxidant activity in Pulicaria undulata (L.) CA Mey from Iran. J Med Plant Res 2011; 5(10): 2035-40. google scholar
- 21. El-Kamali HH, Mahjoub SAT. Antibacterial activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo against seven standard pathogenic bacteria. Ethnobot Leafl 2009; 13: 722-33. google scholar
- 22. Ali NAA, Crouch RA, Al-Fatimi MA, Arnold N, Teichert A, Setzer WN, et al. Chemical composition, antimicrobial, antiradical and anticholinesterase activity of the essential oil of Pulicaria stephanocarpa from Soqotra. Nat Prod Commun 2012; 7(1): 1136. [CrossRef] google scholar
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- 25. Nickavar B, Mojab F. Antibacterial activity of Pulicaria dysenterica extracts. Fitoterapia 2003; 74(4): 390-3. [CrossRef] google scholar
- 26. Touati N, Saidani K, Boudries H, Hammiche H, Ouazene N, Bedjou F. Antibacterial activity of phenolic compounds of Pulicaria odora, wild plant in northern Algeria. Int Food Res J 2018; 25(5): 2021-30. google scholar
- 27. Naqvi SAR, Shah SMA, Kanwal L, Saeed M, Atta-ul-Haq, Ni-sar J, et al. Antimicrobial and antihypercholesterolemic ac-tivities of Pulicaria gnaphalodes. Dose-Response 2020; DOI: 10.1177/1559325820904858. [CrossRef] google scholar
- 28. Zhanzhaxina AS, Seiilgazy M, Jalmakhanbetova RI, Ishmurato-va MY, Seilkhanov TM, Oyama M, et al. Flavonoids from Pulicaria vulgaris and their antimicrobial activity. Chem Nat Compd 2020; 56(5): 915-7. [CrossRef] google scholar
- 29. Abdel Bar FM, Elsbaey M, Taha N, Elgaml A, Abdel-Fattah GM. Phytochemical, antimicrobial and antiquorum-sensing studies of Pulicaria undulata L.:Arevision on the structureof10, 2a, 30,19a, 23-pentahydroxy-urs-12-en-28-oic acid. Nat Prod Res 2020; 34(6): 804-9. [CrossRef] google scholar
- 30. Foudah AI, Alam A, Soliman GA, Salkini MA, Ahmed EI, Yusufog-lu HS. Pharmacognostical, antioxidant and antimicrobial studies of aerial part of Pulicaria crispa (Family: Asteraceae). Bull Environ Pharmacol Life Sci 2015; 4(12): 19-27. [CrossRef] google scholar
- 31. El-Shahaby O, El-Zayat M, Rabei R, Aldesuquy HS. Phytochemical constituents, antioxidant activity and antimicrobial potential of Pulicaria incisa (lam.) DC as a folk medicinal plant. Prog Chem Bio-chem Res 2019; 2(4): 222-7. [CrossRef] google scholar
- 32. Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 2005; 29(1): 41-7. google scholar
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Article |
| Authors | |
| Project Number | Project number: 29555. |
| Publication Date | December 8, 2021 |
| Submission Date | August 24, 2021 |
| Published in Issue | Year 2021 Volume: 11 Issue: 3 |
