Research Article
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Year 2022, Volume: 5 Issue: (Ek sayı 1), 135 - 143, 30.12.2022
https://doi.org/10.46239/ejbcs.1150114

Abstract

Supporting Institution

Mevcut değil

Project Number

Yok

Thanks

Yok

References

  • Ahangarzadeh M, Najafabadi MG, Peyghan R, Houshmand H, Rohani MS, Soltani M. 2022. Detection and distribution of virulence genes in Aeromonas hydrophila isolates causing infection in cultured carps. VRF, 13 (1): 55-60. doi: 10.30466/vrf.2020.115998.2761
  • Austin B, Austin DA. 2007. Isolation / Detection In: Dobbins P (ed) Bacterial fish pathogens Diseases of farmed and wild fish, 4th edn. Springer-Praxis Edinburgh
  • Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. AJCP. 45, 493-496
  • Conte D, Palmeiro JK, Bavaroski AA, Rodrigues LS, Cardozo D, Tomaz AP, Camargo JO, Dalla-Costa LM. 2020. Antimicrobial resistance in Aeromonas species isolated from aquatic environments in Brazil. JAM, 1-14. doi: 10.1111/jam.14965
  • CLSI M49. 2006. Methods for broth dilution susceptibility testing of bacteria isolated from aquatic animals: approved guideline. Clinical and Laboratory Standards Institute, USA.
  • Dhankar J, Kadian SS, Sharma A. 2012. Astaxanthin: a potential carotenoid. JPSR. 3 (5): 1246-1259
  • Dinos PG, Athanasspoulos CM, Missiri DA, Giannopoulou PC, Ulachogiannis IA, Papadopoulos GE, Papaiannou D, Kalpaxis DL. 2016. Chloramphenicol derivatives as antibacterial and anticancer agents: historic problems and current solutions. Antibiotics, 5(20): 1-21. doi: 10.3390/antibiotics5020020
  • Duman M. 2017. Gökkuşağı alabalıklarında görülen motil Aeromonas (Aeromonas hydrophila, A. sobria, A. caviae), Yersinia ruckeri ve Lactococcus garvieae bakterilerinin antimikrobiyal duyarlılıkları ve duyarlılıkta rol oynayan genlerin tespiti. Doktora tezi, Uludağ Üniversitesi, Sağlık Bilimleri Enstitüsü Su Ürünleri Hastalıkları ABD, Bursa, 126 sayfa
  • Ebied MA, Elebshehy EM, Sherif AH, Elgohary M, Turky HA. 2022. Prevalance of antibiotic-resistant Aeromonas hydrophila isolated from the farmed striped mullet Mugil cephalus. EJABF. 26(2). 383-398
  • Ehinmidu JO. 2003. Antibiotic susceptibility patterns of urine bacterial isolates in Zaria, Nigeria. TJPR. 2(2): 223-228
  • Eid HM, El-Mahallawy HS, Shalaby AM, Elsheshtawy HM, Shetewy MM, Eidaroos NH. 2022. Emergency of extensively drug-resistant Aeromonas hydrophila complex isolated from Mugil cephalus (stripped mullet) and Mediterranean seawater. Vet World, 15(1): 55-64.
  • El-Bahar MH, Ali GN, Aboyadak MI, Abd El Salam K, Ibrahim SM. 2019. Virulence genes contributing to Aeromonas hydrophila pathogenicity in Oreochromis niloticus. Int Microbiol 22: 479-490
  • El-Gohary FA, Zahran E, El-Gohary AH, Abdelhamid FM, El-Mleeh A, Elmahallawy EK, Elsayed MM. 2020. Investigation of the prevalence, virulence genes, and antibiogram of motile aeromonads isolated from Nile tilapia fish farmers in Egypt and assessment of their water quality. Ani. 10(1432): 1-16. doi: 10.3390/ani/0081432
  • Fernández-Bravo A., Figueras MJ. 2020. An update on the genus Aeromonas: Taxonomy, epidemiology, and pathogenicicty. Microorganisms. 8(129): 1-39. doi: 10.3390/microorganisms8010129
  • Gardenia L, Koesharyani I, Supriyadi H, Mufidah T. 2010. Aplikasi deteksi dengan menggunakan polymerase chain reaction (PCR). Prosiding FITA. 877-883.
  • Guz L, Nowakiewics A, Puk K, Zreba P, Gnat S, Matuszewski L. 2021. Virulence and antimicrobial resistance pattern of Aeromonas spp. colonizing European pond turtles Emys orbicularis and their natural envirronment. First study from Poland. Animals. 11(2772): 1-14. doi: 10.3390/ani11102772
  • Hoel S, Vadstein C, Jakobsen AN. 2017. Species distribution and prevalance of putative virulence factors in mesophilic Aeromonas spp. isolated from fresh retail sushi. Front. Microbiol. 8(531). doi: 10.3389/fmicb.2017.00931
  • Hossain S, Heo GJ. 2020. Ornamental fish: a potential source of pathogenic and multidrug resistant motile Aeromonas spp. Lett. Appl. Microbiol. 72: 1-12
  • Hossain S, De Silva BCJ, Dahanayake PS, Zoysa MD, Heo GJ. 2020. Phylogenetic characteristics virulence properties and antibiogram profile of motile Aeromonas spp. isolated from ornamental guppy (Poecilia reticulata). Arch. Microbiol. 202: 501-509
  • Joseph AV, Sasidharan RS, Nair NP, Bhat SG. 2013. Occurence of potential pathogenic Aeromonas species in tropical seafood, aquafarms and mangroves off Cochin coast in South India. Vet Worl. 6(6): 300-306. doi: 10.5455/vetworld2013
  • Krumperman PH. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol. 46: 165-170
  • Lotfi A, Soleimani M, Ghasemi N. 2021. Astaxanthin reduces demyelination and oligodendrocytes death in a rat model of multiple sclerosis. Cell J. 22(4): 565-571
  • Marinho YF, Malafaia CB, Araujo KS, da Silva TD, Santos APF, Moraes LB, Gálvaz AO. 2021. Evaluation of the influence of different culture media reon growth, life cycle, biochemical composition, and astaxanthin production in Haematococcus pluvialis. Aquac Int. 29: 757-778
  • Mohd-Aris A, Muhamed Sofie MHN, Zamri-Saad M, Daud HM, Ina-Salwany MY. 2019. Live vaccines against bacterial fish diseases: A review. Vet World. 12 (11): 1806-1815
  • Montaya JM, Mata SV, Acosta JL, Cabiera BEH, Valdez LGL, Reyes C, Cureňo HJB. 2021. Obtaining of astaxanthin from crab exosqueletons and shrimp head shells. Biointerface Res Appl Chem. 11(5): 13516-13523
  • Natarajan M, Kumar D, Mandal J, Biswal N, Stephen S. 2018. A study of virulence and antimicrobial resistance pattern in diarrhoeagenic Escherichia coli isolated from diarrhoeal stool specimens from children and adults in a tertiary hospital, Puducherry, India. J Health Popul Nutr. 37(17): 1-11
  • Odeyemi OA, Asmat A, Usup G. 2012. Antibiotic resistance and putative virulence factors of Aeromonas hydrophila isolated from estuary. J Microbiol Biotechnol Food Sci. 1 (6): 1339-1357
  • Olaizola M. 2017. The production and health benefits of astaxanthin. Nutraceutical Science and Technology. 321-342.
  • Pandey G. 2018. Fish pharmacology and toxicology. Day Publishing House, New Delhi, India
  • Robertson BK, Harden C, Selvaraju SB, Pradhan S, Yadav JS. 2014. Molecular detection, quantificcation, and toxicity profiling of Aeromonas spp. in source-and drinking-water. Open Microbiol J. 8: 32-39.
  • Saharia PK, Hussain IA, Pokhrel H, Kalita B, Borah G, Yasmin R. 2021. Prevalence of motile Aeromonas septicaemia (MAS) in fish culture systems of the central Brahmaputra Valley Zone of Assam, India. Aquac. 52: 1201-1214
  • Sreedharan K, Philip R, Singh ISB. 2012. Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health. Brz J Microbiol. 754-765.
  • Temizkan G, Arda N. 2004. Moleküler biyolojide kullanılan yöntemler. BİYOGEM Yayın no: 2. Nobel Tıp Kitapevleri.
  • Yadav S, Verma DK, Pradhan PK, Dobriyal AK, Sood N. 2014. Phenotypic and genomic identification of Aeromonas species from aquatic environment. Int J Fish Aquat Sci. 5(1): 3-20

Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp.

Year 2022, Volume: 5 Issue: (Ek sayı 1), 135 - 143, 30.12.2022
https://doi.org/10.46239/ejbcs.1150114

Abstract

In the study, in addition to the antibacterial effect of astaxanthin on Aeromonas hydrophila and A. sobria strains, the presence of virulence genes (Aero, act, ast, and hylA) and antibiotic resistance genes (tetC and sulI) in the strains was investigated. Antibiotic profiles of the strains were also investigated as part of the study. Strains were identified by conventional biochemical tests and PCR assay using a 16S rDNA primer pair specific for A. hydrophila. According to the results of bacteriological and molecular studies, two of the six Aeromonas strains were identified as A. hydrophila and four of them as A. sobria. The Aero virulence gene and the act virulence gene were found in all strains, while the ast and hylA virulence genes were detected only in A. hydrophila strains. All strains were resistant to chloramphenicol, tetracycline, nalidixic acid, and ampicillin in the standard disk diffusion test. Although all strains showed resistance to tetracycline and moderate resistance to oxytetracycline in the antibiogram tests, tetC antibiotic resistance gene was not detected in the strains and sulI antibiotic resistance gene was not detected in the strains. In the study, acetone solutions containing 0.1 g and 0.5 g of astaxanthin were found to have an antibacterial effect on A. hydrophila strains. Acetone solutions containing 0.1 g, 0.5 g, and 1.0 g of astaxanthin showed antibacterial effects on A. sobria strains. It was found that 0.1 g, 0.5 g, and 1.0 g astaxanthin solutions prepared with methanol and distilled water had no antibacterial effects on the strains.

Project Number

Yok

References

  • Ahangarzadeh M, Najafabadi MG, Peyghan R, Houshmand H, Rohani MS, Soltani M. 2022. Detection and distribution of virulence genes in Aeromonas hydrophila isolates causing infection in cultured carps. VRF, 13 (1): 55-60. doi: 10.30466/vrf.2020.115998.2761
  • Austin B, Austin DA. 2007. Isolation / Detection In: Dobbins P (ed) Bacterial fish pathogens Diseases of farmed and wild fish, 4th edn. Springer-Praxis Edinburgh
  • Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. AJCP. 45, 493-496
  • Conte D, Palmeiro JK, Bavaroski AA, Rodrigues LS, Cardozo D, Tomaz AP, Camargo JO, Dalla-Costa LM. 2020. Antimicrobial resistance in Aeromonas species isolated from aquatic environments in Brazil. JAM, 1-14. doi: 10.1111/jam.14965
  • CLSI M49. 2006. Methods for broth dilution susceptibility testing of bacteria isolated from aquatic animals: approved guideline. Clinical and Laboratory Standards Institute, USA.
  • Dhankar J, Kadian SS, Sharma A. 2012. Astaxanthin: a potential carotenoid. JPSR. 3 (5): 1246-1259
  • Dinos PG, Athanasspoulos CM, Missiri DA, Giannopoulou PC, Ulachogiannis IA, Papadopoulos GE, Papaiannou D, Kalpaxis DL. 2016. Chloramphenicol derivatives as antibacterial and anticancer agents: historic problems and current solutions. Antibiotics, 5(20): 1-21. doi: 10.3390/antibiotics5020020
  • Duman M. 2017. Gökkuşağı alabalıklarında görülen motil Aeromonas (Aeromonas hydrophila, A. sobria, A. caviae), Yersinia ruckeri ve Lactococcus garvieae bakterilerinin antimikrobiyal duyarlılıkları ve duyarlılıkta rol oynayan genlerin tespiti. Doktora tezi, Uludağ Üniversitesi, Sağlık Bilimleri Enstitüsü Su Ürünleri Hastalıkları ABD, Bursa, 126 sayfa
  • Ebied MA, Elebshehy EM, Sherif AH, Elgohary M, Turky HA. 2022. Prevalance of antibiotic-resistant Aeromonas hydrophila isolated from the farmed striped mullet Mugil cephalus. EJABF. 26(2). 383-398
  • Ehinmidu JO. 2003. Antibiotic susceptibility patterns of urine bacterial isolates in Zaria, Nigeria. TJPR. 2(2): 223-228
  • Eid HM, El-Mahallawy HS, Shalaby AM, Elsheshtawy HM, Shetewy MM, Eidaroos NH. 2022. Emergency of extensively drug-resistant Aeromonas hydrophila complex isolated from Mugil cephalus (stripped mullet) and Mediterranean seawater. Vet World, 15(1): 55-64.
  • El-Bahar MH, Ali GN, Aboyadak MI, Abd El Salam K, Ibrahim SM. 2019. Virulence genes contributing to Aeromonas hydrophila pathogenicity in Oreochromis niloticus. Int Microbiol 22: 479-490
  • El-Gohary FA, Zahran E, El-Gohary AH, Abdelhamid FM, El-Mleeh A, Elmahallawy EK, Elsayed MM. 2020. Investigation of the prevalence, virulence genes, and antibiogram of motile aeromonads isolated from Nile tilapia fish farmers in Egypt and assessment of their water quality. Ani. 10(1432): 1-16. doi: 10.3390/ani/0081432
  • Fernández-Bravo A., Figueras MJ. 2020. An update on the genus Aeromonas: Taxonomy, epidemiology, and pathogenicicty. Microorganisms. 8(129): 1-39. doi: 10.3390/microorganisms8010129
  • Gardenia L, Koesharyani I, Supriyadi H, Mufidah T. 2010. Aplikasi deteksi dengan menggunakan polymerase chain reaction (PCR). Prosiding FITA. 877-883.
  • Guz L, Nowakiewics A, Puk K, Zreba P, Gnat S, Matuszewski L. 2021. Virulence and antimicrobial resistance pattern of Aeromonas spp. colonizing European pond turtles Emys orbicularis and their natural envirronment. First study from Poland. Animals. 11(2772): 1-14. doi: 10.3390/ani11102772
  • Hoel S, Vadstein C, Jakobsen AN. 2017. Species distribution and prevalance of putative virulence factors in mesophilic Aeromonas spp. isolated from fresh retail sushi. Front. Microbiol. 8(531). doi: 10.3389/fmicb.2017.00931
  • Hossain S, Heo GJ. 2020. Ornamental fish: a potential source of pathogenic and multidrug resistant motile Aeromonas spp. Lett. Appl. Microbiol. 72: 1-12
  • Hossain S, De Silva BCJ, Dahanayake PS, Zoysa MD, Heo GJ. 2020. Phylogenetic characteristics virulence properties and antibiogram profile of motile Aeromonas spp. isolated from ornamental guppy (Poecilia reticulata). Arch. Microbiol. 202: 501-509
  • Joseph AV, Sasidharan RS, Nair NP, Bhat SG. 2013. Occurence of potential pathogenic Aeromonas species in tropical seafood, aquafarms and mangroves off Cochin coast in South India. Vet Worl. 6(6): 300-306. doi: 10.5455/vetworld2013
  • Krumperman PH. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol. 46: 165-170
  • Lotfi A, Soleimani M, Ghasemi N. 2021. Astaxanthin reduces demyelination and oligodendrocytes death in a rat model of multiple sclerosis. Cell J. 22(4): 565-571
  • Marinho YF, Malafaia CB, Araujo KS, da Silva TD, Santos APF, Moraes LB, Gálvaz AO. 2021. Evaluation of the influence of different culture media reon growth, life cycle, biochemical composition, and astaxanthin production in Haematococcus pluvialis. Aquac Int. 29: 757-778
  • Mohd-Aris A, Muhamed Sofie MHN, Zamri-Saad M, Daud HM, Ina-Salwany MY. 2019. Live vaccines against bacterial fish diseases: A review. Vet World. 12 (11): 1806-1815
  • Montaya JM, Mata SV, Acosta JL, Cabiera BEH, Valdez LGL, Reyes C, Cureňo HJB. 2021. Obtaining of astaxanthin from crab exosqueletons and shrimp head shells. Biointerface Res Appl Chem. 11(5): 13516-13523
  • Natarajan M, Kumar D, Mandal J, Biswal N, Stephen S. 2018. A study of virulence and antimicrobial resistance pattern in diarrhoeagenic Escherichia coli isolated from diarrhoeal stool specimens from children and adults in a tertiary hospital, Puducherry, India. J Health Popul Nutr. 37(17): 1-11
  • Odeyemi OA, Asmat A, Usup G. 2012. Antibiotic resistance and putative virulence factors of Aeromonas hydrophila isolated from estuary. J Microbiol Biotechnol Food Sci. 1 (6): 1339-1357
  • Olaizola M. 2017. The production and health benefits of astaxanthin. Nutraceutical Science and Technology. 321-342.
  • Pandey G. 2018. Fish pharmacology and toxicology. Day Publishing House, New Delhi, India
  • Robertson BK, Harden C, Selvaraju SB, Pradhan S, Yadav JS. 2014. Molecular detection, quantificcation, and toxicity profiling of Aeromonas spp. in source-and drinking-water. Open Microbiol J. 8: 32-39.
  • Saharia PK, Hussain IA, Pokhrel H, Kalita B, Borah G, Yasmin R. 2021. Prevalence of motile Aeromonas septicaemia (MAS) in fish culture systems of the central Brahmaputra Valley Zone of Assam, India. Aquac. 52: 1201-1214
  • Sreedharan K, Philip R, Singh ISB. 2012. Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health. Brz J Microbiol. 754-765.
  • Temizkan G, Arda N. 2004. Moleküler biyolojide kullanılan yöntemler. BİYOGEM Yayın no: 2. Nobel Tıp Kitapevleri.
  • Yadav S, Verma DK, Pradhan PK, Dobriyal AK, Sood N. 2014. Phenotypic and genomic identification of Aeromonas species from aquatic environment. Int J Fish Aquat Sci. 5(1): 3-20
There are 34 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Research Articles
Authors

Jale Korun

Aycan Ulutaş This is me 0000-0002-4892-6326

Project Number Yok
Publication Date December 30, 2022
Acceptance Date October 9, 2022
Published in Issue Year 2022 Volume: 5 Issue: (Ek sayı 1)

Cite

APA Korun, J., & Ulutaş, A. (2022). Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp. Eurasian Journal of Biological and Chemical Sciences, 5((Ek sayı 1), 135-143. https://doi.org/10.46239/ejbcs.1150114
AMA Korun J, Ulutaş A. Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp. Eurasian J. Bio. Chem. Sci. December 2022;5((Ek sayı 1):135-143. doi:10.46239/ejbcs.1150114
Chicago Korun, Jale, and Aycan Ulutaş. “Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas Spp”. Eurasian Journal of Biological and Chemical Sciences 5, no. (Ek sayı 1) (December 2022): 135-43. https://doi.org/10.46239/ejbcs.1150114.
EndNote Korun J, Ulutaş A (December 1, 2022) Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp. Eurasian Journal of Biological and Chemical Sciences 5 (Ek sayı 1) 135–143.
IEEE J. Korun and A. Ulutaş, “Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp”., Eurasian J. Bio. Chem. Sci., vol. 5, no. (Ek sayı 1), pp. 135–143, 2022, doi: 10.46239/ejbcs.1150114.
ISNAD Korun, Jale - Ulutaş, Aycan. “Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas Spp”. Eurasian Journal of Biological and Chemical Sciences 5/(Ek sayı 1) (December 2022), 135-143. https://doi.org/10.46239/ejbcs.1150114.
JAMA Korun J, Ulutaş A. Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp. Eurasian J. Bio. Chem. Sci. 2022;5:135–143.
MLA Korun, Jale and Aycan Ulutaş. “Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas Spp”. Eurasian Journal of Biological and Chemical Sciences, vol. 5, no. (Ek sayı 1), 2022, pp. 135-43, doi:10.46239/ejbcs.1150114.
Vancouver Korun J, Ulutaş A. Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp. Eurasian J. Bio. Chem. Sci. 2022;5((Ek sayı 1):135-43.