Abstract
References
- AL-BATAINA, B.A., et al. (2003), Element analysis and biological studies on ten oriental spices using XRF and Ames test, J. Trace Elem, Med. Biol., s17(2), 85-90.
- ALBERTINI, R.J., et al. (2000), IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety, Mutat. Res., s463, s72-s111.
- AYDIN, S., et al. (2005), The effects of thyme volatiles on the induction of DNA damage by the heterocyclic amine IQ and mitomycin C, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, s581(1-2), 43-53.
- AZIRAK, S. (2007), Thymol ve Carvacrol’un İn Vivo Genotoksik Etkilerinin Araştırılması, Doktora Tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü.
- AZIZAN, A., and BLEVINS, R.D. (1995), Mutagenicity and antimutagenicity testing of six chemicals associated with the pungent properties of spesific spices as revealed by the Ames Salmonella/microsomal assay, Arch. Environ. Contam.Toxicol., s28(2), 248-58.
- DORMAN, H.J., et al. (2004), Antioxidant properties of aqueous extracts from selected lamiaceae species grown in Turkey, J. Agric. Food. Chem., s52(4), 762-770.
- ELIANA, A.V., et al. (2002), “Genotoxicity of Brosimum gaudichaudii measured by the salmonella/microsome assay and chromosomal aberrations in CHO cells”, J. Ethnopharm, s81, s257-s264.
- GÜL, S., et al. (2012), Chemical composition, and in vitro cytotoxic, genotoxic effects of essential oil from Urtica dioica L., J. B. Environ. Contam. Tox., s88(5), 666-671.
- GÜL, S., et al. (2009), Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro, J. Cytotechnology, s59(2), 113-119.
- GÜLEY, M., and VURAL, N. (1975), Toksikoloji Laboratuvar Kitabı, ISBN: 975-482-289-1, Ankara Eczacılık Fakültesi Yayınları, Ankara.
- KLAASSEN, CD., et al. (2001), Casarett and Doull's Toxicology: The Basic Science of Poisons, (6th ed.), New York, McGraw-Hill.
- LEAL-CARDOSO, J.H., and FONTELES, M.C. (1999), Pharmacological Effects of Essential Oils of Plants of the Northeast of Brazil, An Acad. Bras. Cienc., s71(2), s 207-s213.
- MILLARD, L.G. (1973), Contact sensitivity to toothpaste, Br. Med. J., 1(5854), s.676.
- MURUGAN, S.S., et al. (2007), Antimutagenic effect of broccoli flower head by the ames salmonella reverse mutation assay, Phytother. Res., s21(6), s545 -s547.
- PAZ-Y-MINO, C., et al. (2002), Cytogenetic monitoring in a population occupationally exposed to pesticides in ecuador, J. Environ. Health Perspective. s110, 1077-1080.
- SAVAGE, J.R.K. (1999), An introduction to chromosomal aberretions, Atlas of Genet. Cytogenet. İn Oncol. Haematol.
- SHAH, M., et al. (1996), Contact allergy in patients with oral symptoms: A study of 47 patients, Am. J. Contact Derm., s07, 146-151.
- STAMMATI. S., et al. (1999), Toxicity of selected plant volatiles in microbial and mammalian short-term assays, Food Chem Toxicol., s37(8), 813-23.
- ŞARER, E. (1991) “Uçucu Yağların Biyolojik Etkileri ve Tedavide Kullanımları”, 9. Bitkisel İlaç Hammaddeleri Toplantısı, Bildiriler Kitapçığı, Eskişehir.
- TÜYLÜ, B.A. (2001), Bazı ilaç öncül maddelerinin mutajenik etkilerinin bakteriyal ve hücre kültürü testleriyle araştırılması, Doktora Tezi, Anadolu Üniversitesi, Fen Bilimleri Enstitüsü, Eskişehir.
Investigation of effects of essential oils of Cumin (cuminum cyminum) and Wild Thyme (thymbra spicata) on human lymphocyte chromosomes
Abstract
The aim of this study was to investigate the genotoxic and cytotoxic effects of essential oils of T.spicata and C. cyminum on human peripheral blood lymphocytes. Human peripheral blood lymphocyte cells were treated with 0.05μl/ml, 0.10μl/ml, 0.15μl/ml, 0.20μl/ml concentrations of essential oils of T.spicata and C. cyminum for 24 h. A significant increase was observed for induction of chromosomal aberration frequency in all treatments of essential oils of T.spicata and C. Cyminum concentrations for 24 h comparing with the negative control and mitomycin C (MMC, 0.3µg/ml) which was used as positive control. Chromatid and chromosome breaks, sister chromatid union, fragment and chromatid exchange were observed in all treatment concentrations. Essential oils of T.spicata and C. Cyminum decreased the mitotic index (MI) in all the concentrations and treatment times when compared with control and solvent control. Our results suggest that essential oils of T.spicata and C. cyminum have clastogenic potential for human lymphocytes chromosomes in-vitro.
Keywords
T.spicata C. cyminum mitomycin C (MMC) lymphocyte cultures human chromosomes chromosome aberrations mitotic index
References
- AL-BATAINA, B.A., et al. (2003), Element analysis and biological studies on ten oriental spices using XRF and Ames test, J. Trace Elem, Med. Biol., s17(2), 85-90.
- ALBERTINI, R.J., et al. (2000), IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety, Mutat. Res., s463, s72-s111.
- AYDIN, S., et al. (2005), The effects of thyme volatiles on the induction of DNA damage by the heterocyclic amine IQ and mitomycin C, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, s581(1-2), 43-53.
- AZIRAK, S. (2007), Thymol ve Carvacrol’un İn Vivo Genotoksik Etkilerinin Araştırılması, Doktora Tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü.
- AZIZAN, A., and BLEVINS, R.D. (1995), Mutagenicity and antimutagenicity testing of six chemicals associated with the pungent properties of spesific spices as revealed by the Ames Salmonella/microsomal assay, Arch. Environ. Contam.Toxicol., s28(2), 248-58.
- DORMAN, H.J., et al. (2004), Antioxidant properties of aqueous extracts from selected lamiaceae species grown in Turkey, J. Agric. Food. Chem., s52(4), 762-770.
- ELIANA, A.V., et al. (2002), “Genotoxicity of Brosimum gaudichaudii measured by the salmonella/microsome assay and chromosomal aberrations in CHO cells”, J. Ethnopharm, s81, s257-s264.
- GÜL, S., et al. (2012), Chemical composition, and in vitro cytotoxic, genotoxic effects of essential oil from Urtica dioica L., J. B. Environ. Contam. Tox., s88(5), 666-671.
- GÜL, S., et al. (2009), Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro, J. Cytotechnology, s59(2), 113-119.
- GÜLEY, M., and VURAL, N. (1975), Toksikoloji Laboratuvar Kitabı, ISBN: 975-482-289-1, Ankara Eczacılık Fakültesi Yayınları, Ankara.
- KLAASSEN, CD., et al. (2001), Casarett and Doull's Toxicology: The Basic Science of Poisons, (6th ed.), New York, McGraw-Hill.
- LEAL-CARDOSO, J.H., and FONTELES, M.C. (1999), Pharmacological Effects of Essential Oils of Plants of the Northeast of Brazil, An Acad. Bras. Cienc., s71(2), s 207-s213.
- MILLARD, L.G. (1973), Contact sensitivity to toothpaste, Br. Med. J., 1(5854), s.676.
- MURUGAN, S.S., et al. (2007), Antimutagenic effect of broccoli flower head by the ames salmonella reverse mutation assay, Phytother. Res., s21(6), s545 -s547.
- PAZ-Y-MINO, C., et al. (2002), Cytogenetic monitoring in a population occupationally exposed to pesticides in ecuador, J. Environ. Health Perspective. s110, 1077-1080.
- SAVAGE, J.R.K. (1999), An introduction to chromosomal aberretions, Atlas of Genet. Cytogenet. İn Oncol. Haematol.
- SHAH, M., et al. (1996), Contact allergy in patients with oral symptoms: A study of 47 patients, Am. J. Contact Derm., s07, 146-151.
- STAMMATI. S., et al. (1999), Toxicity of selected plant volatiles in microbial and mammalian short-term assays, Food Chem Toxicol., s37(8), 813-23.
- ŞARER, E. (1991) “Uçucu Yağların Biyolojik Etkileri ve Tedavide Kullanımları”, 9. Bitkisel İlaç Hammaddeleri Toplantısı, Bildiriler Kitapçığı, Eskişehir.
- TÜYLÜ, B.A. (2001), Bazı ilaç öncül maddelerinin mutajenik etkilerinin bakteriyal ve hücre kültürü testleriyle araştırılması, Doktora Tezi, Anadolu Üniversitesi, Fen Bilimleri Enstitüsü, Eskişehir.
Details
| Primary Language | English |
|---|---|
| Journal Section | makaleler |
| Authors | |
| Publication Date | September 4, 2015 |
| Published in Issue | Year 2015 Volume: 1 Issue: 2 |
