Abstract
İntermediyer filamanlar kökeni ve hücre tipi farklı proteinler tarafından oluşturulan hücre iskeleti bileşenlerine ait proteinlerdir. Bu proteinlerin testiste; Sertoli, peritübüler miyoid ve Leydig hücrelerinde bulunduğu bilinmektedir. Bu çalışmanın amacı, testis fizyolojisi için önemli olan ve testisteki hücre iskeleti yapısına katılan sitokeratin 8 proteininin; İran, Ankara ve Van ırkı kedilerin testis dokusundaki lokalizasyonlarını immunohistokimya tekniğine göre ortaya koymaktır. Araştırmada sağlıklı yavru (dokuz çift) ve yetişkin (dokuz çift) kedilerden alınan doku örnekleri kullanıldı. Alınan doku örnekleri rutin histolojik işlemlerin ardından immunohistokimyasal streptavidin-biyotin-peroksidaz kompleks boyama metoduna tabi tutuldu. Elde edilen boyama sonuçlarında, yavru ve yetişkin kedilerde incelenen alanlarda sitokeratin 8 immunoreaktivitelesinin ırklara göre farklı olmadığı görüldü. Bununla birlikte intersitisyel dokuda ve Leydig hücrelerinde immunoreaktivitenin negatif olduğu fakat ertoli ve germ hücrelerinde ise çekirdek etrafında şekillendiği görüldü. Ayrıca epididimis ve deferens kanallarının epitellerinde de sitoplazma ve çekirdekte pozitif immunreaksiyonun olduğu belirlendi. Elde edilen bulgular sonucunda sitokeratin 8’in olgun kedilerde bir kısım farklılıkları türe özgü oluşturabildiği ve kedi testislerindeki bazı hücrelerin iskelet yapısına katılarak spermatogenezisin olgunlaşması, korunması ve spermlerin taşınması gibi önemli roller üstlenebileceği düşünülmüştür.
Keywords
References
- 1. Cheville JC, Rao S, Iczkowski KA, Lohse CM, V. Pankratz VS. (2000). Cytokeratin Expression in Seminoma of The Human Testis. Am J Clin Pathol. 113:583-588.
- 2. Tamai Y, Ishikawa T, Bösl MR et al. (2000). Cytokeratins 8 and 19 In The Mouse Placental Development. The Journal of Cell Biology. 151(3): 563-572
- 3. Yılmaz S. (2021). A Novel Epithelial and Fibroblastic Cell Isolation and Purification Method Using Primary Culture of Bovine Tongue Tissue. Kafkas Univ Vet Fak Derg. 27(6): 675-680.
- 4. Wagner MS, Wajner SM, Maia AL. (2008). The Role of Thyroid Hormone In Testicular Development and Function. J Endocrinol. 199(3): 351-365.
- 5. Mruk DD, Cheng CY. (2002). Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis. Endocrine Reviews. 25(5):747-806.
- 6. Topaloğlu U, Akbalık ME, Sağsöz H. (2021). Immunolocalization of Some HOX Proteins In Immature and Mature Feline Testes. Anatomia, Histologia, Embryologia. 50: 726-735.
- 7. Çelenk F, Saruhan BG, Akbalık ME, Topaloğlu, Aydın N, Bayram B. (2021). Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniv Vet Fak Derg. 14(2):83-88.
- 8. Beyaz F, Bayram GK, Alan E. (2009). Vimentin, Sitokeratin, α-SMA ve Desmin’in Yeni Zellanda Tavşanı Testis ve Epididimisindeki İmmunohistokimyasal Ekspresyonu. Erciyes Üniv Vet Fak Derg. 6(2): 111-119.
- 9. Miguel MP, Bethencourt FR, Arenas MI, Fraile B, Paniagua R. (1997). Intermediate Filaments In The Sertoli Cells of The Ageing Human Testis. Virchows Arch. 431:131-138.
- 10. Santiago J, Silva JV, Alves MG, Oliveira PF, Fardilha M. (2019). Testicular Aging: An Overview of Ultrastructural, Cellular, and Molecular Alterations. J Gerontol A Biol Sci Med Sci. 74 (6): 860-871.
- 11. Amlani S, Vogl AW. (1988). Changes in the Distribution of Microtubules and Intermediate Filaments in Mammalian Sertoli Cells During Spermatogenesis. The Anatomical Record. 220:143-160.
- 12. Steger K, Schimmel M, Wrobel KH. (1994). Immunohistochemical Demonstration of Cytoskelatal Proteins In Seminiferous Tubules of Adult Rams and Bulls. Arch Histol Cytol. 57(1): 17-28.
- 13. Wakui S, Furusato M, Shinichiro U, Kano Y. (1994). Coexpression of Different Cytokeratins, Vimentin and Desmin In The Rete Testis and Epididymis nn the Dog. J Anat. 184: 147-151.
- 14. Akbalik ME, Sagsoz H, Erdogan S. (2015). Osteopontin Epression in the Intestine of Chukar Partridge (Alectoris chukar, Gray, 1830). Animal Biology. 65(3-4): 287-298.
- 15. Dunleavy JEM, O’Bryan MK, Stanton PG, O’Donnell L. (2019). The Cytoskeleton in Spermatogenesis. Reproduction. 157 (2): R53-R72.
- 16. Stosiek P, Kasper M, Karsten U. (1990). Expression of Cytokeratins 8 and 18 in Human Sertoli Cells of Immature and Atrophic Seminiferous Tubules. Differentiation. 43(1): 66-70.
- 17. Lehtonen E. (1985). A Monoclonal Antibody Against Mouse Oocyte Cytoskeleton Recognizing Cytokeratin-Type Filaments. J Embryo1 Exp Morpho. l90:197-209.
- 18. Park YJ, Kim JH, Kim HY et al. (2020). The Expression and Localization of V-ATPase and Cytokeratin 5 during Postnatal Development of The Pig Epididymis. Asian-Australas J Anim Sci Vol. 33(7) :1077-1086.
- 19. Pecile A, Groppetti D, Pizzi G et al. (2021). Immunohistochemical Insights into a Hidden Pathology: Canine cryptorchidism. Theriogenology. 176: 43-53.
- 20. Rogatsch H, Jezek D, Mikuz AHG, Feichtinger H. (1996). Expression of Vimentin, Cytokeratin, and Desmin in Sertoli Cells of Human Fetal, Cryptorchid, and Tumour-Adjacent Testicular Tissue. Virchows Arch. 427:497-502.
- 21. Dinges HP, Zatloukal K, Schmid C, Mair S, Wirnsberger G. (1991). Co-Expression of Cytokeratin and Vimentin Filaments İn Rete Testis and Epididymis. Virchows Archiv A Pathol Anat. 418:119-127.
- 22. Kasper M, Stosiek P. (1989). Immunohistochemical Investigation of Different Cytokeratins and Vimentin İn The Human Epididymis from The Fetal Period up to Adulthood. Cell Tissue Res. 257:661-664.
- 23. Regadera J, España G, Roias MA, Recio JA, Nistal M, Suárez-Quian CA. (1997). Morphometric and Immunocytochemical Study of the Fetal, Infant, and Adult Human Vas Deferens. Journal of Andrology. 18(6): 623-636.
Abstract
Intermediate filaments are proteins belonging to cytoskeletal components formed by proteins of different origin and cell type. These proteins are in the testis; Sertoli, peritubular myoid and Leydig cells are known to exist. The aim of this study is to determine cytokeratin 8 protein, which is important for testicular physiology and participates in the cytoskeletal structure of the testis; To reveal the localization of Iranian, Ankara and Van breed cats in testicular tissue using immunohistochemistry technique. Tissue samples from healthy kittens (nine pairs) and adult (nine pairs) cats were used in the study. Tissue samples were subjected to immunohistochemical streptavidin-biotin-peroxidase complex staining method after routine histological procedures. In the staining results obtained, it was seen that cytokeratin 8 immunoreactivity in the examined areas in kittens and adult cats did not differ according to breeds. However, it was observed that immunoreactivity was negative in interstitial tissue and Leydig cells, but formed around the nucleus in Sertoli and germ cells. In addition, it was determined that there was a positive immunoreaction in the cytoplasm and nucleus in the epithelium of the epididymis and deferens ducts. As a result of the findings obtained, it was thought that cytokeratin 8 may cause some differences in mature cats and may play important roles such as the maturation of spermatogenesis, protection and transport of sperm by participating in the skeletal structure of some cells in the cat testicles.
Keywords
References
- 1. Cheville JC, Rao S, Iczkowski KA, Lohse CM, V. Pankratz VS. (2000). Cytokeratin Expression in Seminoma of The Human Testis. Am J Clin Pathol. 113:583-588.
- 2. Tamai Y, Ishikawa T, Bösl MR et al. (2000). Cytokeratins 8 and 19 In The Mouse Placental Development. The Journal of Cell Biology. 151(3): 563-572
- 3. Yılmaz S. (2021). A Novel Epithelial and Fibroblastic Cell Isolation and Purification Method Using Primary Culture of Bovine Tongue Tissue. Kafkas Univ Vet Fak Derg. 27(6): 675-680.
- 4. Wagner MS, Wajner SM, Maia AL. (2008). The Role of Thyroid Hormone In Testicular Development and Function. J Endocrinol. 199(3): 351-365.
- 5. Mruk DD, Cheng CY. (2002). Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis. Endocrine Reviews. 25(5):747-806.
- 6. Topaloğlu U, Akbalık ME, Sağsöz H. (2021). Immunolocalization of Some HOX Proteins In Immature and Mature Feline Testes. Anatomia, Histologia, Embryologia. 50: 726-735.
- 7. Çelenk F, Saruhan BG, Akbalık ME, Topaloğlu, Aydın N, Bayram B. (2021). Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniv Vet Fak Derg. 14(2):83-88.
- 8. Beyaz F, Bayram GK, Alan E. (2009). Vimentin, Sitokeratin, α-SMA ve Desmin’in Yeni Zellanda Tavşanı Testis ve Epididimisindeki İmmunohistokimyasal Ekspresyonu. Erciyes Üniv Vet Fak Derg. 6(2): 111-119.
- 9. Miguel MP, Bethencourt FR, Arenas MI, Fraile B, Paniagua R. (1997). Intermediate Filaments In The Sertoli Cells of The Ageing Human Testis. Virchows Arch. 431:131-138.
- 10. Santiago J, Silva JV, Alves MG, Oliveira PF, Fardilha M. (2019). Testicular Aging: An Overview of Ultrastructural, Cellular, and Molecular Alterations. J Gerontol A Biol Sci Med Sci. 74 (6): 860-871.
- 11. Amlani S, Vogl AW. (1988). Changes in the Distribution of Microtubules and Intermediate Filaments in Mammalian Sertoli Cells During Spermatogenesis. The Anatomical Record. 220:143-160.
- 12. Steger K, Schimmel M, Wrobel KH. (1994). Immunohistochemical Demonstration of Cytoskelatal Proteins In Seminiferous Tubules of Adult Rams and Bulls. Arch Histol Cytol. 57(1): 17-28.
- 13. Wakui S, Furusato M, Shinichiro U, Kano Y. (1994). Coexpression of Different Cytokeratins, Vimentin and Desmin In The Rete Testis and Epididymis nn the Dog. J Anat. 184: 147-151.
- 14. Akbalik ME, Sagsoz H, Erdogan S. (2015). Osteopontin Epression in the Intestine of Chukar Partridge (Alectoris chukar, Gray, 1830). Animal Biology. 65(3-4): 287-298.
- 15. Dunleavy JEM, O’Bryan MK, Stanton PG, O’Donnell L. (2019). The Cytoskeleton in Spermatogenesis. Reproduction. 157 (2): R53-R72.
- 16. Stosiek P, Kasper M, Karsten U. (1990). Expression of Cytokeratins 8 and 18 in Human Sertoli Cells of Immature and Atrophic Seminiferous Tubules. Differentiation. 43(1): 66-70.
- 17. Lehtonen E. (1985). A Monoclonal Antibody Against Mouse Oocyte Cytoskeleton Recognizing Cytokeratin-Type Filaments. J Embryo1 Exp Morpho. l90:197-209.
- 18. Park YJ, Kim JH, Kim HY et al. (2020). The Expression and Localization of V-ATPase and Cytokeratin 5 during Postnatal Development of The Pig Epididymis. Asian-Australas J Anim Sci Vol. 33(7) :1077-1086.
- 19. Pecile A, Groppetti D, Pizzi G et al. (2021). Immunohistochemical Insights into a Hidden Pathology: Canine cryptorchidism. Theriogenology. 176: 43-53.
- 20. Rogatsch H, Jezek D, Mikuz AHG, Feichtinger H. (1996). Expression of Vimentin, Cytokeratin, and Desmin in Sertoli Cells of Human Fetal, Cryptorchid, and Tumour-Adjacent Testicular Tissue. Virchows Arch. 427:497-502.
- 21. Dinges HP, Zatloukal K, Schmid C, Mair S, Wirnsberger G. (1991). Co-Expression of Cytokeratin and Vimentin Filaments İn Rete Testis and Epididymis. Virchows Archiv A Pathol Anat. 418:119-127.
- 22. Kasper M, Stosiek P. (1989). Immunohistochemical Investigation of Different Cytokeratins and Vimentin İn The Human Epididymis from The Fetal Period up to Adulthood. Cell Tissue Res. 257:661-664.
- 23. Regadera J, España G, Roias MA, Recio JA, Nistal M, Suárez-Quian CA. (1997). Morphometric and Immunocytochemical Study of the Fetal, Infant, and Adult Human Vas Deferens. Journal of Andrology. 18(6): 623-636.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research |
| Authors | |
| Publication Date | June 30, 2022 |
| Acceptance Date | May 18, 2022 |
| Published in Issue | Year 2022 Volume: 15 Issue: 1 |
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DANIŞMA KURULU
Prof. Dr. Abit AKTAŞ, İstanbul Üniversitesi-Cerrahpaşa
Prof. Dr. Yılmaz ARAL, Ankara Üniversitesi
Prof. Dr. Ali ARSLAN, Fırat Üniversitesi, Elazığ
Prof. Dr. M. Sedat BARAN, Dicle Üniversitesi, Diyarbakır
Prof. Dr. Cengiz CEYLAN, Balıkesir Üniversitesi
Prof. Dr. Ahmet ÇAKIR, Ankara Üniversitesi
Prof. Dr. Burhan ÇETİNKAYA, Fırat Üniversitesi, Elazığ
Prof. Dr. Ülker EREN, Adnan Menderes Üniversitesi, Aydın
Prof. Dr. Abdurrahman GÜL, Bingöl Üniversitesi
Prof. Dr. Hakki KARA, Cumhuriyet Üniversitesi, Sivas
Prof. Dr. Narin LİMAN, Erciyes Üniversitesi, Kayseri
Prof. Dr. Abdullah ÖZEN, Fırat Üniversitesi, Elazığ
Prof. Dr. Ayşe SERBEST, Uludağ Üniversitesi, Bursa
Prof. Dr. Kazım ŞAHİN, Fırat Üniversitesi, Elazığ
Prof. Dr. Tekin ŞAHİN, Siirt Üniversitesi
Prof. Dr. Nihat ŞINDAK, Siirt Üniversitesi
Prof. Dr. Muzaffer TAŞ, Namık Kemal Üniversitesi, Tekirdağ
Prof. Dr. Necati TİMURKAN Fırat Üniversitesi, Elazığ
Prof. Dr. Emine ÜNSALDI, Fırat Üniversitesi, Elazığ
Prof. Dr. Murat YILDIRIM, Kırıkkale Üniversitesi
Doç. Dr. M. Osman ATLI, Harran Üniversitesi, Şanlıurfa
Doç. Dr. Bülent ELİTOK, Afyon Kocatepe Üniversitesi
Doç. Dr. Serkan ERDOĞAN, Namık Kemal Üniversitesi, Tekirdağ
Dr. Öğr. Üyesi Zelal KARAKOÇ, Dicle Üniversitesi, Diyarbakır
Dr. Öğr. Üyesi Ersin UYSAL, Dicle Üniversitesi, Diyarbakır
*Not: Yayın ve Danışma Kurulu Üyeleri alfabetik sıraya göre dizilmiştir.