Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Elif Mutlu; Hasan H.s. Abuıyada

Review

Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Year 2021, Volume: 10 Issue: 1, 95 - 107, 10.06.2021

Elif Mutlu Hasan H.s. Abuıyada

Abstract

Organotipik beyin kesit kültürleri günümüzde nörobiyoloji araştırmalarında rutin bir protokol haline gelmiştir. Beyin kesit kültürü tekniklerindeki ilerleme sayesinde birçok beyin hastalığı patofizyolojisinin in vivo duruma çok yakın bir şekilde doku bağlamında incelenmesi fırsatı doğmuştur. In vivo çalışmalarda anesteziklerin ve kas gevşetici maddelerin uzun süreli kullanımına alternatif olması yanında hücre kültürlerinin ve homojenatlarının aksine yapısal bütünlüğünü sürdürebilmesi gibi avantajlar sunarken bu kültür ortamı bazı dezavantajları da beraberinde getirmektedir. Bu derleme çalışması boyunca beyin kesit kültürü teknolojisi avantajları ve dezavantajları ile birlikte ele alınarak nörobiyoloji çalışmalarındaki yeri incelenmiştir. Hücresel bütünlüğün korunduğu bu sistemlerin nörodejenerasyon, nörogenez, nörotoksisite gibi birçok alanda in vitro hücre kültürü ve in vivo deney hayvanı çalışmalarına kıyasla çok daha verimli ve kullanışlıdır.

Keywords

Beyin, Kesit kültürü, Organotipik beyin kesiti, Nörobiyoloji

References

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Use of Organotypic Brain Slices in Neurobiological Studies

Year 2021, Volume: 10 Issue: 1, 95 - 107, 10.06.2021

Elif Mutlu Hasan H.s. Abuıyada

Abstract

Organotypic brain slice cultures have now become a routine protocol in neurobiology researches. Thanks to the advancement in brain section culture techniques, the opportunity has emerged to examine the pathophysiology of many brain diseases in a tissue context very close to the in vivo situation. In addition to being an alternative to the long-term use of anesthetics and muscle relaxants in in vivo studies, it offers advantages such as maintaining the structural integrity of cell cultures and homogenates, while this culture environment also brings some disadvantages. Throughout this review study, brain slice culture technology was considered together with its advantages and disadvantages and its place in neurobiology studies was examined. These systems, in which cellular integrity is preserved, are much more efficient and useful in many areas such as neurodegeneration, neurogenesis, neurotoxicity compared to in vitro cell culture and in vivo experimental animal studies.

Keywords

Brain, Slice culture, Organotypic brain slice, Neurobiology

References

D. D. Clarke and L. Sokoloff, Circulation and energy metabolism of the brain. In: Sigel GJ, Agrano BW, Albers RW, Fisher SK and Uhler MD (eds.), Basic Neurochemistry: Molecular, Cellular and Medical Aspects. Philadelphia, Lippincott-Raven, 1999, pp. 637-669.
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G. N. Elston, R. Benavides-Piccione, and J. DeFelipe, “The Pyramidal Cell in Cognition: A Comparative Study in Human and Monkey,” Journal Neurosci, vol. 21, no. 17, pp. RC163–RC163, Sep. 2001, doi: https://doi.org/10.1523/JNEUROSCI.21-17-j0002.2001.
T. Branco and M. Häusser, “Synaptic Integration Gradients in Single Cortical Pyramidal Cell Dendrites,” Neuron, vol. 69, no. 5, pp. 885–892, Mar. 2011, doi: 10.1016/j.neuron. 2011.02.006.
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B. A. Bahr, “Long-term hippocampal slices: A model system for investigating synaptic mechanisms and pathologic processes,” J. Neurosci. Res, vol. 42, no. 3, pp. 294–305, Oct. 1995, doi: 10.1002/jnr.490420303.
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J. G. Mielke, T. Comas, J. Woulfe, R. Monette, B. Chakravarthy and G.A.R Mealing, “Cytoskeletal, synaptic, and nuclear protein changes associated with rat interface organotypic hippocampal slice culture development.” Brain Res. Dev. Brain Res, vol. 160, no. 2, pp. 275–86, 2005, doi: 10.1016/j.devbrainres.2005.09.009.
K. Duff, W. Noble, K. Gaynor, and Y. Matsuoka, “Organotypic Slice Cultures from Transgenic Mice as Disease Model Systems,” J Mol Neurosci, vol. 19, no. 3, pp. 317–320, 2002, doi: 10.1385/JMN:19:3:317.
J. Noraberg, B. W. Kristensen, and J. Zimmer, “Markers for neuronal degeneration in organotypic slice cultures,” Brain Res. Brain Res. Protoc, vol. 3, no. 3, pp. 278–290, Jan. 1999, doi: 10.1016/s1385-299x(98)00050-6.
C. L. Croft, H. S. Futch, B. D. Moore, and T. E. Golde, “Organotypic brain slice cultures to model neurodegenerative proteinopathies,” Mol. Neurodegener, vol. 14, no. 1, Dec. 2019, doi: 10.1186/s13024-019-0346-0.
M. Finley, D. Fairman, D. Liu, P. Li, A. Wood, and S. Cho, “Functional validation of adult hippocampal organotypic cultures as an in vitro model of brain injury,” Brain Research, vol. 1001, no. 1–2, pp. 125–132, Mar. 2004, doi: 10.1016/j.brainres.2003.12.009.
S. Cho, A. Wood, and M. Bowlby, “Brain Slices as Models for Neurodegenerative Disease and Screening Platforms to Identify Novel Therapeutics,” Curr Neuropharmacol, vol. 5, no. 1, pp. 19–33, Mar. 2007, doi: 10.2174/157015907780077105.
K.H. Adcock, F. Metzger and J.P. Kapfhammer, “Purkinje cell dendritic tree development in the absence of excitatory neurotransmission and of brain-derived neurotrophic factor in organotypic slice cultures,” Neuroscience, May 2004, doi: 10.1016/j.neuroscience. 2004.04.032.
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Primary Language	Turkish
Subjects	Structural Biology
Journal Section	Review Article
Authors	Elif Mutlu 0000-0002-2580-4043 Hasan H.s. Abuıyada 0000-0002-5525-779X
Publication Date	June 10, 2021
Submission Date	March 24, 2021
Published in Issue	Year 2021 Volume: 10 Issue: 1

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IEEE	E. Mutlu and H. H. Abuıyada, “Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri”, DUFED, vol. 10, no. 1, pp. 95–107, 2021.

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