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ANTIOXIDANT AND PHYSIOLOGICAL ANALYSIS OF TRITICALE UNDER COLD ACCLIMATION CONDITIONS IN VITRO AND EX VITRO

Year 2020, Volume: 29 Issue: 2, 338 - 350, 31.12.2020

Abstract

Triticale is an artificial species that originated about 130 years ago from between wheat and rye, and the first commercially viable cultivars were released in the 1960s. The crop exhibits high yield, promising long term potential, elevated grain quality, and better resistance to pathogens, desirable amino acid content, and high adaptation ability to adverse climate conditions. Sudden decreases in climate can pose significant losses in many crops including Triticale. Understanding plant response to cold acclimation could help developing crops resilient to cold. In this study, we aim to compare the antioxidants and physiological content of Triticale under cold acclimation in vitro and ex vitro. In our study, five triticale cultivars, Ümran Hanım, Alper Bey, Mikham 2002, Tatlıcak, and Melez 2001 were used as the plant material. Triticale seeds were planted in 15 cm sand pods. They were maintained in 20/180C (day / night) greenhouse with a 12 h day length for 10 days to initiation germination. After 2 weeks the plants were transferred at 4±10C for cold acclimation for 30 days. Callus was transferred to a hormone-free MS medium for 1 month. All cultures were kept under fluorescent light with 15000 lux and 16 h/8 h light/dark cycle at 25±10C. The culture media was subsequently refreshed and kept under fluorescent light with 1500 lux and 16 h/8 h light/dark cycle at 4±10C under cold acclimation. Our results revealed that the cold acclimation changed the activities of APX (Ascorbate Peroxidase), SOD (Superoxide Dismutase), and CAT (Catalase) under both ex vitro and in vitro conditions. The highest correlation between enzyme activities and cold resistance was observed in the sugar content of in vitro stress callus. Our results indicated as closely related to proline, sugar content and antioxidant enzyme activities at cold acclimation in the evaluation of cold tolerance of Triticale cultivars.

Supporting Institution

Erzurum Technical University Review Board

Project Number

Project no: 263 BAP 2015/10

References

  • Jha, A.C., Bohra, A., Jha, R., Breeding approaches and genomics technologies to increase crop yield under low-temperature stress, Plant Cell Rep, 36 (2017), 1–35.
  • Pearce, R. S., Plant freezing and damage, Ann Bot., 87 (2001) , 417–424.
  • Shepherd, T. G., Effects of a warming, Arctic. Science, 353 (2016), 989 990.
  • Theocharis, A., Clément, C., Barka, E.A., Physiological and molecular changesin plants grown at low temperatures, Planta, 235 (2012), 1091–1105.
  • Korner, C., Plant adaptation to cold climates, F1000Res, 5 (2016), 2769– 2774.
  • Maurya, J. P., Bhalerao, R. P., Photoperiod and temperature-mediated control of growth cessation and dormancy in trees a molecular perspective, Ann. Bot., 120 (2017), 351-360.
  • Lokhande, V.H., Nikam, T.D., Penna, S., Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L, Plant Cell Tissue Organ Culture, 102 (2010), 17-25.
  • Karimi, R., Ershadi, A., Abdolhossein, R. N., Khanizadeh, S., Abscisic acid alleviates the deleterious effects of cold stress on ‘Sultana’ grapevine (Vitis vinifera L.) plants by improving the anti-oxidant activity and photosynthetic capacity of leaves, J. Hortic. Sci. 301 Biotechnol, 91/2 (2016), 1-10.
  • Tasgin, E., Atıcı, Ö., Nalbantoglu, B., Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves, Plant Growth regulation, 41 (2003), 231–236.
  • Wise, R.R., Chilling-enhanced photooxidation the production, action and study of reactive oxygen species produced during chilling in the light, Photosynth Res., 45 (1995), 79–349 97.
  • Lee, D.H., Lee, C.B., Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber in gel enzyme activity assays, Plant Sci, 159 (2000), 75–85.
  • Hills, M.J., Hall, L.M., Messenger, D.F., Graf, R.J., Beres, B.L.,Eudes, F., Evaluation of crossability between Triticale (Triticosecale Wittmack) and common wheat, durum wheat and rye, Environ Biosaf Res., 6/4 (2007), 249–257.
  • Blum, A., The abiotic stress response and adaptation of Triticale a review, Cereal Res Commun., 42 (2014), 359–375.
  • Ramirez-Garcia, J., Gabriel, J.L., Alonso-Ayuso, M., Quemada, M., Quantitative characterization of five cover crop species, The Journal of Agricultural Sciences, 153(7) (2015), 1174-1185.
  • Dorosieve, L., Plant cell and tissue culture present state and future prospects, Genetical Selektasiya, 19(5) (1996), 356-362.
  • Karp, S. S. H., Parmar, S., Jones, M. G. K., Shewry, P. R., Breiman, A., Relative stability among barley plants regenerated from cultured immature embryos, Genome, 29 (1998), 405-412.
  • Upadhyaya, G., Sen, M., Roy, A., Comparative studies of in vitro and in vivo raised seedlings of Oryza sativa L, Indian J. Sci. Res., 5/2 (2014), 109-117.
  • Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant, 15 (1962), 473–497.
  • Bates, L., Waldren, R. P., Teare, D., Rapid determination of free proline for water stress studies, Plant Soil, 39 (1973), 205–207.
  • Ortiz-Marchane, M.I., Teresa Ruiz, M., Valverde, F., Romero, J. M., Determination of soluble sugars Arabidopsis thaliana leaves by anion exchange chromatography, Bio-protocol, 4 (2014), 23.
  • Gracjana, L., Kamil, T. F., Paulina, Z. P., Ireneusz, S., Beata, M. K., The activity of superoxide dismutases (SODs) at the early stages of wheat deetiolation, Research Article, 10 (2018), 1371.
  • Gong, Y., Toivonen, O., Lau, L., Wiersma, P. A., Antioxidant system level in ‘Braeburn’ apple is related to its broning disorder, Bot. Bull. Acad. Sin., 42 (2001), 259-264.
  • Andreia, C., Gisele, P., Silvia, B. R., Carolina, W. R., Fermanda, L., Marcia, M. P., Plant responses to stresses role of ascorbate peroxidase in the antioxidant protection, Genetics and Molecular Biology, 35 (2012), 1011-1019.
  • Kosova, K., Prasil, I. T., Vitamvas, P., Dobrev, P., Motyka, V., Flokova, K., Complex phytohormone responses during the cold acclimation of two wheat cultivars differing in cold tolerance, winter Samanta and spring Sandra, J. Plant Physiol., 169 (2010), 567-576.
  • Cherian, S., Reddy, M. P., Evaluation of NaCl tolerance in the callus cultures of Suaeda nudiflora Moq, Biol. Plant., 46 (2003), 193-198.
  • Zhao, M. G., Chen, L., Zhang, L.L., Zhang, W.H., Nitric reductase-dependent nitric oxideproduction is involved in cold acclimation and freezing tolerance in Arabidopsis, Plant Physiol., 151 (2009), 55-767.
  • Wisniewski, M., Bassett, C., Gusta, L.V., An overview of cold hardiness in woody plants seeing the forest through the trees, Hort Science, 38 (2003), 952–959.
  • Liu, Y., Dang, P., Liu, L., He, C., Cold acclimation by the CBF–COR pathway in a changing climate lessons from Arabidopsis thaliana, Plant Cell Reports, 38 (2019), 511-519.
  • Karp, S. S. H., Parmar, S., Jones, M. G. K., Shewry, P. R., Breiman, A., Relative stability among barley plants regenerated from cultured immature embryos, Genome, 29 (1987), 405-412.
  • Alam, J., Imran, M., Hassan, L., Rubel, M.H., Shamsuddoha, M. In vitro regeneration of high yielding Indica Rice (Oryza sativa L.) varieties, J. Environ. Sci. 271 & Natural Resources, 5/1 (2012), 173-177.
  • İsmail, B., Response of five Triticale genotypes to salt stress in in vitro culture, Turkish of agriculture and forestry, 41/5 (2017), 372-380.
  • Vera Hernandez, F. P., Martineznunez, M., Ruiz Rivas, M., Vazuez Portillo, R., Bibbins, M.D., Martinez, S., Suarez, L., De, F., Rosas Cardenas, F., Reference genes for RT-PCR normalisation different tissues, developmental stages and stress conditions of amaranth, Plant biology, 1 (2018),1-12.
  • Esim, N., Atici, O., Nitric oxide improves chilling tolerance of maize by affecting apoplastic antioxidative enzymes in leaves, Plant Growth Regul., 72 (2014), 29–38.
  • Smirnoff, N., Cumbes, Q.J., Hydroxyl radical scavenging activity of compatible solutes, Phytochemistry, 28 (1989), 1057–1060.
  • Zhang, J., Jiang, F., Yang, P., Li, J., Yan, G., Hu, L., Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria, Acta Physiol. Plant., 37 (2015), 7.10.1007/s11738-014-1748-9.
  • Erdal, S., Genisel, M., Turk, H., Dumlupinar, R., Demir, Y., Modulation of alternative oxidase to enhance tolerance against cold stress of chickpea by chemical treatments, Journal of Plant Physiology, 175 (2015), 5-101.
  • Lugato, D., Simao, M.J., Garcia, R., Mansur, E., Pacheco, G., Determination of antioxidant activity and phenolic content of extracts from in vivo plants and in vitro materials of Passiflora Alata Curtis, Plant Cell, Tissue and Organ Culture (PCTOC), 118/2 (2014), 339-346.
  • Mittler, R., Oxidative stress, antioxidants and stress tolerance, Trends in Plant Science, 7 (2002), 405-410.
  • Joudmand, A., Roghieh, H., Silicon mitigates cold stress in barley plants via modifying the activity of apoplasmic enzymes and concentration of metabolites, Acta Physiologiae Plantarum, (2019), 41-29.
  • Ozkur, O., Ozdemir, F., Bor, M., Turkan, I., Physiochemical and antioxidant responses of the perennial xerophyte Capparis ovata Desf. to drought, Environmental and Experimental Botany, 66 (2009), 487-492.
  • Baek, K.H., Skinner, D.Z., Alternation of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines, Plant Science, 165 (2013), 1221-1227.
  • Balestrasse, K.B., Tomaro, M.L., Batlle, A., Noriega, G.O., The role of 5-aminolevulinic acid in the response to cold stress in soybean plants, Phytochemistry, 276 (71) (2010), 17-18.
  • Javadian, N., Karimzadeh, G., Mahfoozi, S., Ghanati, F., Cold-induced changes of enzymes, proline, carbohydrates, and chlorophyll in wheat, Russian Journal of Plant 294 Physiology, 57 (2010), 540-547.
Year 2020, Volume: 29 Issue: 2, 338 - 350, 31.12.2020

Abstract

Project Number

Project no: 263 BAP 2015/10

References

  • Jha, A.C., Bohra, A., Jha, R., Breeding approaches and genomics technologies to increase crop yield under low-temperature stress, Plant Cell Rep, 36 (2017), 1–35.
  • Pearce, R. S., Plant freezing and damage, Ann Bot., 87 (2001) , 417–424.
  • Shepherd, T. G., Effects of a warming, Arctic. Science, 353 (2016), 989 990.
  • Theocharis, A., Clément, C., Barka, E.A., Physiological and molecular changesin plants grown at low temperatures, Planta, 235 (2012), 1091–1105.
  • Korner, C., Plant adaptation to cold climates, F1000Res, 5 (2016), 2769– 2774.
  • Maurya, J. P., Bhalerao, R. P., Photoperiod and temperature-mediated control of growth cessation and dormancy in trees a molecular perspective, Ann. Bot., 120 (2017), 351-360.
  • Lokhande, V.H., Nikam, T.D., Penna, S., Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L, Plant Cell Tissue Organ Culture, 102 (2010), 17-25.
  • Karimi, R., Ershadi, A., Abdolhossein, R. N., Khanizadeh, S., Abscisic acid alleviates the deleterious effects of cold stress on ‘Sultana’ grapevine (Vitis vinifera L.) plants by improving the anti-oxidant activity and photosynthetic capacity of leaves, J. Hortic. Sci. 301 Biotechnol, 91/2 (2016), 1-10.
  • Tasgin, E., Atıcı, Ö., Nalbantoglu, B., Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves, Plant Growth regulation, 41 (2003), 231–236.
  • Wise, R.R., Chilling-enhanced photooxidation the production, action and study of reactive oxygen species produced during chilling in the light, Photosynth Res., 45 (1995), 79–349 97.
  • Lee, D.H., Lee, C.B., Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber in gel enzyme activity assays, Plant Sci, 159 (2000), 75–85.
  • Hills, M.J., Hall, L.M., Messenger, D.F., Graf, R.J., Beres, B.L.,Eudes, F., Evaluation of crossability between Triticale (Triticosecale Wittmack) and common wheat, durum wheat and rye, Environ Biosaf Res., 6/4 (2007), 249–257.
  • Blum, A., The abiotic stress response and adaptation of Triticale a review, Cereal Res Commun., 42 (2014), 359–375.
  • Ramirez-Garcia, J., Gabriel, J.L., Alonso-Ayuso, M., Quemada, M., Quantitative characterization of five cover crop species, The Journal of Agricultural Sciences, 153(7) (2015), 1174-1185.
  • Dorosieve, L., Plant cell and tissue culture present state and future prospects, Genetical Selektasiya, 19(5) (1996), 356-362.
  • Karp, S. S. H., Parmar, S., Jones, M. G. K., Shewry, P. R., Breiman, A., Relative stability among barley plants regenerated from cultured immature embryos, Genome, 29 (1998), 405-412.
  • Upadhyaya, G., Sen, M., Roy, A., Comparative studies of in vitro and in vivo raised seedlings of Oryza sativa L, Indian J. Sci. Res., 5/2 (2014), 109-117.
  • Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant, 15 (1962), 473–497.
  • Bates, L., Waldren, R. P., Teare, D., Rapid determination of free proline for water stress studies, Plant Soil, 39 (1973), 205–207.
  • Ortiz-Marchane, M.I., Teresa Ruiz, M., Valverde, F., Romero, J. M., Determination of soluble sugars Arabidopsis thaliana leaves by anion exchange chromatography, Bio-protocol, 4 (2014), 23.
  • Gracjana, L., Kamil, T. F., Paulina, Z. P., Ireneusz, S., Beata, M. K., The activity of superoxide dismutases (SODs) at the early stages of wheat deetiolation, Research Article, 10 (2018), 1371.
  • Gong, Y., Toivonen, O., Lau, L., Wiersma, P. A., Antioxidant system level in ‘Braeburn’ apple is related to its broning disorder, Bot. Bull. Acad. Sin., 42 (2001), 259-264.
  • Andreia, C., Gisele, P., Silvia, B. R., Carolina, W. R., Fermanda, L., Marcia, M. P., Plant responses to stresses role of ascorbate peroxidase in the antioxidant protection, Genetics and Molecular Biology, 35 (2012), 1011-1019.
  • Kosova, K., Prasil, I. T., Vitamvas, P., Dobrev, P., Motyka, V., Flokova, K., Complex phytohormone responses during the cold acclimation of two wheat cultivars differing in cold tolerance, winter Samanta and spring Sandra, J. Plant Physiol., 169 (2010), 567-576.
  • Cherian, S., Reddy, M. P., Evaluation of NaCl tolerance in the callus cultures of Suaeda nudiflora Moq, Biol. Plant., 46 (2003), 193-198.
  • Zhao, M. G., Chen, L., Zhang, L.L., Zhang, W.H., Nitric reductase-dependent nitric oxideproduction is involved in cold acclimation and freezing tolerance in Arabidopsis, Plant Physiol., 151 (2009), 55-767.
  • Wisniewski, M., Bassett, C., Gusta, L.V., An overview of cold hardiness in woody plants seeing the forest through the trees, Hort Science, 38 (2003), 952–959.
  • Liu, Y., Dang, P., Liu, L., He, C., Cold acclimation by the CBF–COR pathway in a changing climate lessons from Arabidopsis thaliana, Plant Cell Reports, 38 (2019), 511-519.
  • Karp, S. S. H., Parmar, S., Jones, M. G. K., Shewry, P. R., Breiman, A., Relative stability among barley plants regenerated from cultured immature embryos, Genome, 29 (1987), 405-412.
  • Alam, J., Imran, M., Hassan, L., Rubel, M.H., Shamsuddoha, M. In vitro regeneration of high yielding Indica Rice (Oryza sativa L.) varieties, J. Environ. Sci. 271 & Natural Resources, 5/1 (2012), 173-177.
  • İsmail, B., Response of five Triticale genotypes to salt stress in in vitro culture, Turkish of agriculture and forestry, 41/5 (2017), 372-380.
  • Vera Hernandez, F. P., Martineznunez, M., Ruiz Rivas, M., Vazuez Portillo, R., Bibbins, M.D., Martinez, S., Suarez, L., De, F., Rosas Cardenas, F., Reference genes for RT-PCR normalisation different tissues, developmental stages and stress conditions of amaranth, Plant biology, 1 (2018),1-12.
  • Esim, N., Atici, O., Nitric oxide improves chilling tolerance of maize by affecting apoplastic antioxidative enzymes in leaves, Plant Growth Regul., 72 (2014), 29–38.
  • Smirnoff, N., Cumbes, Q.J., Hydroxyl radical scavenging activity of compatible solutes, Phytochemistry, 28 (1989), 1057–1060.
  • Zhang, J., Jiang, F., Yang, P., Li, J., Yan, G., Hu, L., Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria, Acta Physiol. Plant., 37 (2015), 7.10.1007/s11738-014-1748-9.
  • Erdal, S., Genisel, M., Turk, H., Dumlupinar, R., Demir, Y., Modulation of alternative oxidase to enhance tolerance against cold stress of chickpea by chemical treatments, Journal of Plant Physiology, 175 (2015), 5-101.
  • Lugato, D., Simao, M.J., Garcia, R., Mansur, E., Pacheco, G., Determination of antioxidant activity and phenolic content of extracts from in vivo plants and in vitro materials of Passiflora Alata Curtis, Plant Cell, Tissue and Organ Culture (PCTOC), 118/2 (2014), 339-346.
  • Mittler, R., Oxidative stress, antioxidants and stress tolerance, Trends in Plant Science, 7 (2002), 405-410.
  • Joudmand, A., Roghieh, H., Silicon mitigates cold stress in barley plants via modifying the activity of apoplasmic enzymes and concentration of metabolites, Acta Physiologiae Plantarum, (2019), 41-29.
  • Ozkur, O., Ozdemir, F., Bor, M., Turkan, I., Physiochemical and antioxidant responses of the perennial xerophyte Capparis ovata Desf. to drought, Environmental and Experimental Botany, 66 (2009), 487-492.
  • Baek, K.H., Skinner, D.Z., Alternation of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines, Plant Science, 165 (2013), 1221-1227.
  • Balestrasse, K.B., Tomaro, M.L., Batlle, A., Noriega, G.O., The role of 5-aminolevulinic acid in the response to cold stress in soybean plants, Phytochemistry, 276 (71) (2010), 17-18.
  • Javadian, N., Karimzadeh, G., Mahfoozi, S., Ghanati, F., Cold-induced changes of enzymes, proline, carbohydrates, and chlorophyll in wheat, Russian Journal of Plant 294 Physiology, 57 (2010), 540-547.
There are 43 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Büşra Yazıcılar This is me 0000-0003-2465-7579

Serap Karaman 0000-0003-1216-0012

İsmail Bezirganoglu 0000-0003-4079-5998

Doğan İlhan 0000-0003-2805-1638

Project Number Project no: 263 BAP 2015/10
Publication Date December 31, 2020
Acceptance Date September 30, 2020
Published in Issue Year 2020 Volume: 29 Issue: 2

Cite

Communications Faculty of Sciences University of Ankara Series C-Biology.

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